Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,578,495 A→G 5.1% F78L (TTC→CTC)  gntR ← d‑gluconate inducible gluconate regulon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,578,4950AG 5.1% 373.5 / 8.6 118F78L (TTC→CTC) gntRd‑gluconate inducible gluconate regulon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (62/50);  new base G (3/3);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 120 total aligned reads displayed.

GCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAAC  >  NC_000913/3578361‑3578633
                                                                                                                                      |                                                                                                                                          
gcagTCGATATTCCAGGACAGCATGGATTCGACGCCTTCGTGCACCAATTCCGGTTTATAACCGAAGTGCCCCAGCAAGGTCTGATAACCGTGCGCGACGGTGACGCATTAGAATACGCGTAAAACTTCCGCGAGAACCt                                                                                                                                       >  1:212416/4‑140 (MQ=255)
gCCGTCGATATTCCAGGAGTGCATGGATTCGAGGCGTTCTTGCTCCTTTTCCGGTTTTTAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTTATTCTTCCGCGAAAAcat                                                                                                                                       >  2:1089241/1‑138 (MQ=255)
gCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTATTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCt                                                                                                                                       >  1:1295771/1‑140 (MQ=255)
gCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAGAACCt                                                                                                                                       >  1:212388/1‑140 (MQ=255)
gCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAGAACCt                                                                                                                                       >  1:212459/1‑140 (MQ=255)
gCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCGTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCt                                                                                                                                       >  1:158295/1‑140 (MQ=255)
  cGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCtgg                                                                                                                                     <  2:736243/140‑1 (MQ=255)
    tCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCtggtt                                                                                                                                   >  1:1243923/1‑140 (MQ=255)
         atTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTga                                                                                                                              <  2:146649/140‑1 (MQ=255)
           tCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTgaga                                                                                                                            <  2:165434/140‑1 (MQ=255)
           tCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTgaga                                                                                                                            >  2:1558171/1‑140 (MQ=255)
           tCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTgaga                                                                                                                            >  2:554085/1‑140 (MQ=255)
            ccAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTgagag                                                                                                                           <  1:12006/140‑1 (MQ=255)
               ggAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAg                                                                                                                        >  2:2605/1‑140 (MQ=255)
                gagaGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAgg                                                                                                                       >  1:832818/1‑140 (MQ=255)
                gagaGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAgg                                                                                                                       >  1:40504/1‑140 (MQ=255)
                gagaGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAgg                                                                                                                       >  2:1596120/1‑140 (MQ=255)
                  gagCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTa                                                                                                                     >  1:1049335/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCTGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:866881/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATTGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATACCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:165133/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:992291/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:693008/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:518725/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  2:1436946/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCGTTCGATTCCGCGTAATACTTCCGCGAAATCCTGGGTGGTGAGAGAAGGTAACa                                                                                                                  >  1:992607/1‑140 (MQ=255)
                     cATGGATTCGAGGCGTTCTTGCTACATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGTCGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACa                                                                                                                  >  1:1170232/1‑140 (MQ=255)
                        ggATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGa                                                                                                               <  2:1390241/140‑1 (MQ=255)
                          aTTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGTAGGTAACAGGACg                                                                                                             >  1:1613101/1‑140 (MQ=255)
                            tCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGcc                                                                                                           <  2:1249410/140‑1 (MQ=255)
                               aGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTTATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACTCCAAt                                                                                                        >  2:750095/1‑140 (MQ=255)
                               aGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAAt                                                                                                        >  2:1663096/1‑140 (MQ=255)
                               aGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAAt                                                                                                        >  2:1473877/1‑140 (MQ=255)
                               aGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGACTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAAt                                                                                                        >  2:1663573/1‑140 (MQ=255)
                               aGGCGTTCTTGCTCAATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGAGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACATCCGCGAAAACCTGGTTGGAGAGAGAAGGTAAAAGGACGCCAAt                                                                                                        >  2:750081/1‑140 (MQ=255)
                                    ttCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGccc                                                                                                   >  2:1549724/1‑140 (MQ=255)
                                          gatcATTACCGGTATATATCCGTAGTGCGCCTTCATGCTCAGATAACCGTGCGCGTCGGTGCCGCTTTCGATTGCGCGTATTACTTCCGCGTAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCtgg                                                                                             <  2:1675776/137‑1 (MQ=255)
                                          cTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAGAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCtgg                                                                                             <  2:212416/140‑1 (MQ=255)
                                          cTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAGAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCtgg                                                                                             <  2:212459/140‑1 (MQ=255)
                                           tCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCtggt                                                                                            >  2:870509/1‑140 (MQ=255)
                                               tttCCGGTTTTTAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAATGGTGGTTGGTGAGTGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCg                                                                                        >  2:383796/1‑140 (MQ=255)
                                               tttCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCg                                                                                        >  1:89204/1‑140 (MQ=255)
                                               tttCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCg                                                                                        >  2:615694/1‑140 (MQ=255)
                                               tttCCGGATTATAACCGTAGACCGCCAGCATGGAATGATAACCGTCCGCGTAGCTGACGCTTTCGAATCCGCGTAATACAGCCGCGAAAACCTGGTTGGTGAGAGAAGGTAAAAGGACCCCAATCGCCCGGCTAGTGGCg                                                                                        >  2:615967/1‑140 (MQ=255)
                                                     gTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGTAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTagag                                                                                  <  2:1616194/140‑1 (MQ=255)
                                                     gTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTagag                                                                                  >  1:721530/1‑140 (MQ=255)
                                                     gTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTagag                                                                                  <  2:659241/140‑1 (MQ=255)
                                                     gTTTATAACCGTAGTGCGCCAGCATGGTCAGATAACCGTGCGCGTCGGTGAAGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTagat                                                                                  >  1:685165/1‑139 (MQ=255)
                                                                tAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCggg                                                                       >  1:412679/1‑140 (MQ=255)
                                                                tAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCggg                                                                       >  2:906308/1‑140 (MQ=255)
                                                                tAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCggg                                                                       >  1:471940/1‑140 (MQ=255)
                                                                tAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCggg                                                                       >  2:13719/1‑140 (MQ=255)
                                                                tAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTGGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCggg                                                                       >  1:1115300/1‑140 (MQ=255)
                                                                    gcgcCAGCATTGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATCCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCa                                                                   <  2:165133/140‑1 (MQ=255)
                                                                    gcgcCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCa                                                                   <  1:203769/140‑1 (MQ=255)
                                                                    gcgcCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCa                                                                   <  1:920275/140‑1 (MQ=255)
                                                                      gcCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACg                                                                 <  1:1284320/140‑1 (MQ=255)
                                                                        cAGCATGGTCGGATAACCGTGCGCGTCGGTGACGCTATCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGTAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGAt                                                               <  2:381905/140‑1 (MQ=255)
                                                                         aGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGAtt                                                              <  1:743923/140‑1 (MQ=255)
                                                                          gCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCACTCGCCCGGCTGGTGGCGTTCGAGTGGAGATCGGGCGCACGAGTg                                                             >  2:353811/1‑140 (MQ=255)
                                                                           cATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTgg                                                            <  1:1538631/140‑1 (MQ=255)
                                                                           cATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTgg                                                            <  1:759372/140‑1 (MQ=255)
                                                                           cATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTgg                                                            <  1:464983/140‑1 (MQ=255)
                                                                               gTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACTGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGTTTGGGTat                                                        >  1:1359491/1‑140 (MQ=255)
                                                                               gTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAat                                                        >  1:1359483/1‑140 (MQ=255)
                                                                               gTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTGAGAGAGGATATCGGGCGCACGATTGGGAat                                                        >  1:1358966/1‑140 (MQ=255)
                                                                                tCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAata                                                       <  1:194350/140‑1 (MQ=255)
                                                                                tCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAata                                                       <  1:792345/140‑1 (MQ=255)
                                                                                tCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAata                                                       <  1:49930/140‑1 (MQ=255)
                                                                                     tAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGcc                                                  >  1:793021/1‑140 (MQ=255)
                                                                                     tAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGcc                                                  <  2:606557/140‑1 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  2:255192/1‑140 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  1:748205/1‑140 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  2:1310871/1‑140 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  1:1544704/1‑140 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  1:1029504/1‑140 (MQ=255)
                                                                                          gTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGtt                                             >  1:1033597/1‑140 (MQ=255)
                                                                                           tGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTtc                                            <  2:1011518/140‑1 (MQ=255)
                                                                                           tGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGATAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTtc                                            <  1:1409889/140‑1 (MQ=255)
                                                                                               cgTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTtcatca                                        <  2:928091/140‑1 (MQ=255)
                                                                                                    gTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCTCGATTGGGAATATAGCCCAGTTCATCAAGAGc                                   <  2:1478443/140‑1 (MQ=255)
                                                                                                    gTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGc                                   <  2:1422742/140‑1 (MQ=255)
                                                                                                    gTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGc                                   <  2:690706/140‑1 (MQ=255)
                                                                                                      gACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCcg                                 <  2:1455996/140‑1 (MQ=255)
                                                                                                       aCGCTTTCTAGTCATCGGAATACTTCCCCGAACACCAGGTTGGTTTTAGAACGTTTCAGGACGCCAAACGACAGGCTTGTGGCGTTAGAGAGGATATCGGGCGCTCGATTGGGAATATATGCCAGTTCATCAAGAGCcgc                                <  2:1673850/140‑1 (MQ=255)
                                                                                                       aCGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCcgc                                <  1:1387974/140‑1 (MQ=255)
                                                                                                       aCGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCcgc                                <  2:1135580/140‑1 (MQ=255)
                                                                                                       aCGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCcgc                                <  2:770131/140‑1 (MQ=255)
                                                                                                       aCGATTTCGACTGCGCGGAATACGTCCGCGAAAACCTGGTTGGTGAGCGAATGTAACTGTTCGCCAATCGCCGGGCTGGAGGCGTTAGTGACGTTCTCGTTCGCACGCTTGGGCATATAGCCCTGTTAATCAAGAGgcgc                                <  2:303090/140‑1 (MQ=255)
                                                                                                         gCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCgg                              >  1:58593/1‑140 (MQ=255)
                                                                                                           tttCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCa                            <  2:1052289/140‑1 (MQ=255)
                                                                                                            ttCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCaa                           >  2:790477/1‑140 (MQ=255)
                                                                                                                      cgTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCcgcg                 <  2:89204/140‑1 (MQ=255)
                                                                                                                           tACTTCCGCGAACACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATAGAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTagag            <  2:188504/140‑1 (MQ=255)
                                                                                                                           tACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTagag            <  2:866881/140‑1 (MQ=255)
                                                                                                                                ccGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACg       >  1:39982/1‑140 (MQ=255)
                                                                                                                                ccGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACg       >  1:39983/1‑140 (MQ=255)
                                                                                                                                ccGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACg       <  2:1049335/140‑1 (MQ=255)
                                                                                                                                ccGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACg       >  1:1143159/1‑140 (MQ=255)
                                                                                                                                ccGCGAAAACCTGGTTGGGGAGAGAAGCTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACg       >  2:443185/1‑140 (MQ=255)
                                                                                                                                     aaaaCCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAAc  >  1:39479/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                          
GCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATAACCGTGCGCGTCGGTGACGCTTTCGATTCCGCGTAATACTTCCGCGAAAACCTGGTTGGTGAGAGAAGGTAACAGGACGCCAATCGCCCGGCTGGTGGCGTTAGAGAGGATATCGGGCGCACGATTGGGAATATAGCCCAGTTCATCAAGAGCCGCGGCAATCTTGCCGCGTAGAGCGACGGAAAC  >  NC_000913/3578361‑3578633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: