Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,864,609 G→T 5.9% K237N (AAG→AAT yidP → UTRA domain‑containing GntR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,864,6090GT 5.9% 228.6 / 2.1 68K237N (AAG→AATyidPUTRA domain‑containing GntR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base T (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

GCGGAACACACGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGATTATTAGCAAACGCC  >  NC_000913/3864472‑3864744
                                                                                                                                         |                                                                                                                                       
gCGGAACACACGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAagag                                                                                                                                       <  2:20478/140‑1 (MQ=255)
     acacacGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCTCCCGGAAAAGAGTTAAc                                                                                                                                  <  2:1397959/140‑1 (MQ=255)
      cacacGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGGGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAAcc                                                                                                                                 >  1:784244/1‑140 (MQ=255)
        cacGCCACTGCTGCGGATCACCTCGTTGTCATATCGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGa                                                                                                                               <  1:932370/140‑1 (MQ=255)
        cacGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGa                                                                                                                               <  1:220379/140‑1 (MQ=255)
        cacGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGa                                                                                                                               <  2:433549/140‑1 (MQ=255)
        cacGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGa                                                                                                                               <  2:433556/140‑1 (MQ=255)
             cACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTc                                                                                                                          <  1:677069/140‑1 (MQ=255)
                tgctgcGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCag                                                                                                                       >  2:182255/1‑140 (MQ=255)
                       gATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCa                                                                                                                >  1:1403675/1‑140 (MQ=255)
                       gATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCa                                                                                                                >  2:92019/1‑140 (MQ=255)
                       gATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCa                                                                                                                >  2:1485045/1‑140 (MQ=255)
                          cACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTCACCGATACTCCAGAAGAGCACCg                                                                                                             >  2:826316/1‑140 (MQ=255)
                          cACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTAGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCg                                                                                                             >  2:823916/1‑140 (MQ=255)
                            ccTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGcc                                                                                                           <  2:43080/140‑1 (MQ=255)
                            ccTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGcc                                                                                                           <  2:44680/140‑1 (MQ=255)
                            ccTCGTTGTAATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCATAAGAGCACCGca                                                                                                           >  2:852329/1‑139 (MQ=255)
                               cGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGt                                                                                                        >  1:565522/1‑140 (MQ=255)
                                    tCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTg                                                                                                   <  2:687707/140‑1 (MQ=255)
                                         taGCGAGAGCGGGGAGTATTTGACTTATACAGTGATGAACAGCACTGCCAGCGATTACCAGGAGGAGGACCTTTTACGGCGACTCCACCCGGAACCGAGATAACCGATACTCCAGAAGAGCACCGCCTGTAATTgggggg                                                                                              <  2:1319285/140‑1 (MQ=255)
                                            cGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGa                                                                                           >  1:354554/1‑140 (MQ=255)
                                            cGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGa                                                                                           <  2:1335461/140‑1 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGTAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  2:1433912/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTCATTGGGGGGTGAtc                                                                                         >  1:1671002/1‑139 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:1108954/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  2:1570770/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  2:477463/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:115288/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:1129046/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:1538353/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:321244/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  1:350128/1‑140 (MQ=255)
                                              agagCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCAACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATa                                                                                         >  2:1436351/1‑140 (MQ=255)
                                                agCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGACATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCACAAGAGCACCGCCAGTCATTGGCGGGTGATata                                                                                       >  2:298939/1‑138 (MQ=255)
                                                                attattCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTAcc                                                                       <  2:1474045/140‑1 (MQ=255)
                                                                  tattCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGTGCAGGAACATTACCaa                                                                     <  1:1180648/140‑1 (MQ=255)
                                                                  tattCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCaa                                                                     <  1:349620/140‑1 (MQ=255)
                                                                  tattCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCaa                                                                     <  2:1229521/140‑1 (MQ=255)
                                                                  tattCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCaa                                                                     <  2:446319/140‑1 (MQ=255)
                                                                   attCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAATAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAAc                                                                    >  2:811966/1‑140 (MQ=255)
                                                                   attCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAATAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAAc                                                                    >  2:234012/1‑140 (MQ=255)
                                                                     tCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCTCCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACgg                                                                  <  2:775380/140‑1 (MQ=255)
                                                                           aTGTACAGAAATGCCAGCGAATACCAGGTGGATTAACATTTACGGCGAATACACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATACTTCGCAGGAACATTACCAACGGATAGAc                                                            <  2:320201/140‑1 (MQ=255)
                                                                             gTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  1:1335508/140‑1 (MQ=255)
                                                                             gTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  1:1336053/140‑1 (MQ=255)
                                                                             gTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  1:535951/140‑1 (MQ=255)
                                                                             gTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  1:666323/140‑1 (MQ=255)
                                                                             gTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  2:460453/140‑1 (MQ=255)
                                                                                       gCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAaga                                                >  1:838589/1‑140 (MQ=255)
                                                                                       gCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAaga                                                >  1:838588/1‑140 (MQ=255)
                                                                                          aGCGAATACCCGGTGGAGTACCACTTACGGCGACTCCACCCGGAAAAGAGATAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAagagag                                             <  2:831201/140‑1 (MQ=255)
                                                                                                  ccAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAATAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGcgccgccg                                     <  1:234012/140‑1 (MQ=255)
                                                                                                  ccAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAATAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGcgccgccg                                     <  1:811966/140‑1 (MQ=255)
                                                                                                   cAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCATCGGATAGACAGTGGCGTAAGAGAGcgccgccgc                                    >  1:1689016/1‑140 (MQ=255)
                                                                                                       tGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCg                                <  1:1619111/140‑1 (MQ=255)
                                                                                                       tGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCg                                <  1:739734/140‑1 (MQ=255)
                                                                                                           gaaCCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACACCAGAAGAGCACCGCCAGTAAATGGGGGGTGATATTGCGCAGGAACATTACCAACGCAGAGACAGTGGCGTAAGAGAGCGCCGCCGCACCTCTgg                            >  2:726599/3‑140 (MQ=255)
                                                                                                            tACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGt                           >  2:1245333/1‑140 (MQ=255)
                                                                                                                 ttgaCGGCGACTCCACCCGGACAAGAGTTACGCGGTACTCCAGAAGAGCACCGCCTGTAAGTCGGGGGAGATACAGCGCAGGACCAGACCCAACCGAGAGACAGGCCCGGATGCGAGCGCCGCCGCACCGCTGGGTggcg                      >  2:1596180/4‑138 (MQ=255)
                                                                                                                   tACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGa                    <  1:1550675/140‑1 (MQ=255)
                                                                                                                   tACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGa                    <  2:969011/140‑1 (MQ=255)
                                                                                                                   gccgtcGACTACACCCGGAAAAGAGATAACCGATACTCCAGACGAGCTCCGACACTAATTGGTGTGTGTTAATGATCAGGAACATTACCAACGGATAGCAAGTGCCGTAAGATAGCGCCGCCGCACCGCTGGTTGGATGa                    <  2:877069/135‑1 (MQ=255)
                                                                                                                      ggCGACTCCACCCGGAAAAGAGTCAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGa                 >  1:1010432/1‑140 (MQ=255)
                                                                                                                        cGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGatt               >  2:1283299/1‑140 (MQ=255)
                                                                                                                        cGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGatt               >  2:1211956/1‑140 (MQ=255)
                                                                                                                        cGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGatt               >  1:715016/1‑140 (MQ=255)
                                                                                                                                cccGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          >  2:351230/1‑89 (MQ=255)
                                                                                                                                cccGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAg                                                          <  1:351230/89‑1 (MQ=255)
                                                                                                                                     aaaaGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGATTATTAGCAAACGcc  <  2:1215047/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCGGAACACACGCCACTGCTGCGGATCACCTCGTTGTCATATAGCGAGAGCGGGGAGTTTTTGAATTATTCAGTGATGTTCAGAAATGCCAGCGAATACCAGGTGGAGTACCATTTACGGCGACTCCACCCGGAAAAGAGTTAACCGATACTCCAGAAGAGCACCGCCAGTAATTGGGGGGTGATAATGCGCAGGAACATTACCAACGGATAGACAGTGGCGTAAGAGAGCGCCGCCGCACCGCTGGTTGGATGAAGATTATTAGCAAACGCC  >  NC_000913/3864472‑3864744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: