Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,258,317 G→T 9.2% V192V (GTG→GTT dinF → oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,258,3170GT 9.2% 205.5 / 7.2 65V192V (GTG→GTTdinFoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base T (3/3);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

TCTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGC  >  NC_000913/4258188‑4258455
                                                                                                                                 |                                                                                                                                          
tCTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTct                                                                                                                                  <  2:1085869/140‑1 (MQ=255)
tCTTGAAATCAGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTct                                                                                                                                  <  2:1085866/140‑1 (MQ=255)
 cTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTctg                                                                                                                                 <  2:1027039/140‑1 (MQ=255)
 cTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTctg                                                                                                                                 <  2:1026969/140‑1 (MQ=255)
   tGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTctggc                                                                                                                               <  2:307321/140‑1 (MQ=255)
   tGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTctggc                                                                                                                               <  2:307774/140‑1 (MQ=255)
         ccGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGa                                                                                                                         <  2:52027/140‑1 (MQ=255)
            cTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTGTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATgg                                                                                                                      >  2:1237560/1‑140 (MQ=255)
            cTGGTTAAGCGCACCGGAGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATgg                                                                                                                      >  1:339098/1‑140 (MQ=255)
              ggTTAAGCGCCCCGGCGTCGCTGGCGAAGCAGGTATTACTCGGGTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATgggg                                                                                                                    <  2:1483845/140‑1 (MQ=255)
              ggTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCCATATGCCCGTGCGCCAGTAATTTTGGTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCGGGTGATgggg                                                                                                                    <  2:1070869/140‑1 (MQ=255)
              ggTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATgggg                                                                                                                    <  2:878768/140‑1 (MQ=255)
              ggTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATgggg                                                                                                                    <  1:145195/140‑1 (MQ=255)
                ttAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCt                                                                                                                  <  2:704830/140‑1 (MQ=255)
                        aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                          <  1:901615/140‑1 (MQ=255)
                        aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                          <  1:901250/140‑1 (MQ=255)
                        aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                          >  1:474003/1‑140 (MQ=255)
                         ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                         >  2:456265/1‑140 (MQ=255)
                         ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                         >  1:931102/1‑140 (MQ=255)
                         ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                         >  2:247672/1‑140 (MQ=255)
                         ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACAGTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                         >  2:1148604/1‑140 (MQ=255)
                          cGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAAc                                                                                                        >  2:1591990/1‑140 (MQ=255)
                          cGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAAc                                                                                                        >  1:1473543/1‑140 (MQ=255)
                               tCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCa                                                                                                   <  1:1608041/140‑1 (MQ=255)
                                    ggCGCATCTTGAAGTCCTCCGTTGTCTACTCCGCGTGCACTATGCCCGTGCGCCAGTAATGTCGGCAGTGGGCGGCAAGATCCACCACAGTGAGCTGGATGTCTGTCTGGTGATGGGTCTGCATGTGCACGTGCATggct                                                                                              <  2:496054/140‑2 (MQ=255)
                                    ggCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGggcg                                                                                              <  2:42429/140‑1 (MQ=255)
                                                      cGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTa                                                                            <  1:543669/140‑1 (MQ=255)
                                                      cGGTTGGCTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTa                                                                            <  1:1081624/140‑1 (MQ=255)
                                                        gttggttACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTAtt                                                                          >  2:129087/1‑140 (MQ=255)
                                                        gttggttACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTAtt                                                                          >  1:230317/1‑140 (MQ=255)
                                                               aCTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAat                                                                   >  1:284230/1‑140 (MQ=255)
                                                               aCTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAat                                                                   >  1:284212/1‑140 (MQ=255)
                                                                cTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  <  2:1201901/140‑1 (MQ=255)
                                                                   ggCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGc                                                               >  2:540114/1‑140 (MQ=255)
                                                                     cGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCac                                                             >  2:11007/1‑139 (MQ=255)
                                                                     cGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCaa                                                             >  2:10929/1‑140 (MQ=255)
                                                                     cGTGCAATATGCCCGTGCGCCAGTAATTTTGTGAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATCTGAACGTGCTGGGCGCGGCGCTGGCGACGGTTATTGCGGAATTTGCaa                                                             >  2:132681/1‑140 (MQ=255)
                                                                      gTGCAATATGCCCGTTCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCa                                                                                                               >  1:27430/1‑89 (MQ=255)
                                                                      gTGCAATATGCCCGTTCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCa                                                                                                               <  2:27430/89‑1 (MQ=255)
                                                                       tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACgg                                                                               <  2:144685/120‑1 (MQ=255)
                                                                       tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACgg                                                                               >  1:144685/1‑120 (MQ=255)
                                                                       tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCACCa                                                           >  1:1034060/1‑140 (MQ=255)
                                                                               gCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGAt                                                   >  1:1088073/1‑140 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:315200/140‑1 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:481729/140‑1 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:481844/140‑1 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:1311391/140‑1 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:59530/140‑1 (MQ=255)
                                                                                  cGTGCGCCAGTAATTTTGTTAGTGGACGTCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                <  1:313529/140‑1 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  <  1:1610020/120‑1 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  <  1:1610106/120‑1 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  >  2:610572/1‑120 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  >  2:1610020/1‑120 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  >  2:1610106/1‑120 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                  <  1:610572/120‑1 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                              >  2:1417477/1‑140 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                              >  1:352612/1‑140 (MQ=255)
                                                                                    tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                              >  1:270767/1‑140 (MQ=255)
                                                                                      cgcCAGTAATTTTGTTGGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTg                                            >  2:1267259/1‑140 (MQ=255)
                                                                                      cgcCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTg                                            >  2:189219/1‑140 (MQ=255)
                                                                                           gTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAAt                                       >  2:293649/1‑140 (MQ=255)
                                                                                                    ttAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTAGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTaa                              <  1:1591990/140‑1 (MQ=255)
                                                                                                      aGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTaaaa                            >  2:1570662/1‑140 (MQ=255)
                                                                                                          gTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCt                        >  2:1032129/1‑140 (MQ=255)
                                                                                                                    tCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTAcgc              <  2:1291905/140‑1 (MQ=255)
                                                                                                                              tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg    >  1:34109/1‑140 (MQ=255)
                                                                                                                              tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg    >  1:348426/1‑140 (MQ=255)
                                                                                                                              tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg    >  1:1520851/1‑140 (MQ=255)
                                                                                                                              tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCGAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg    >  1:1126341/1‑140 (MQ=255)
                                                                                                                              tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGGCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATATCCg    >  1:192102/1‑140 (MQ=255)
                                                                                                                               gtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCgg   >  1:1001307/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  2:842722/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  2:915588/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  1:1640099/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  1:1640297/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  2:1653629/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  1:195734/1‑140 (MQ=255)
                                                                                                                                tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc  >  1:847617/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                          
TCTTGAAATCCGCTGGTTAAGCGCACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGC  >  NC_000913/4258188‑4258455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: