Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,258,350 G→T 7.9% M203I (ATG→ATT dinF → oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,258,3500GT 7.9% 255.7 / 5.9 76M203I (ATG→ATTdinFoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base G (44/26);  new base T (3/3);  total (47/29)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGAAA  >  NC_000913/4258212‑4258484
                                                                                                                                          |                                                                                                                                      
aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                                                       <  1:901615/140‑1 (MQ=255)
aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                                                       <  1:901250/140‑1 (MQ=255)
aCCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGa                                                                                                                                       >  1:474003/1‑140 (MQ=255)
 ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                                                      >  2:456265/1‑140 (MQ=255)
 ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                                                      >  1:931102/1‑140 (MQ=255)
 ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                                                      >  2:247672/1‑140 (MQ=255)
 ccGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACAGTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGaa                                                                                                                                      >  2:1148604/1‑140 (MQ=255)
  cGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAAc                                                                                                                                     >  1:1473543/1‑140 (MQ=255)
  cGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAAc                                                                                                                                     >  2:1591990/1‑140 (MQ=255)
       tCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCa                                                                                                                                <  1:1608041/140‑1 (MQ=255)
            ggCGCATCTTGAAGTCCTCCGTTGTCTACTCCGCGTGCACTATGCCCGTGCGCCAGTAATGTCGGCAGTGGGCGGCAAGATCCACCACAGTGAGCTGGATGTCTGTCTGGTGATGGGTCTGCATGTGCACGTGCATggct                                                                                                                           <  2:496054/140‑2 (MQ=255)
            ggCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGggcg                                                                                                                           <  2:42429/140‑1 (MQ=255)
                              cGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTa                                                                                                         <  1:543669/140‑1 (MQ=255)
                              cGGTTGGCTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTa                                                                                                         <  1:1081624/140‑1 (MQ=255)
                                gttggttACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTAtt                                                                                                       >  2:129087/1‑140 (MQ=255)
                                gttggttACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTAtt                                                                                                       >  1:230317/1‑140 (MQ=255)
                                       aCTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAat                                                                                                >  1:284230/1‑140 (MQ=255)
                                       aCTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAat                                                                                                >  1:284212/1‑140 (MQ=255)
                                        cTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               <  2:1201901/140‑1 (MQ=255)
                                           ggCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGc                                                                                            >  2:540114/1‑140 (MQ=255)
                                             cGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCac                                                                                          >  2:11007/1‑139 (MQ=255)
                                             cGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCaa                                                                                          >  2:10929/1‑140 (MQ=255)
                                             cGTGCAATATGCCCGTGCGCCAGTAATTTTGTGAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATCTGAACGTGCTGGGCGCGGCGCTGGCGACGGTTATTGCGGAATTTGCaa                                                                                          >  2:132681/1‑140 (MQ=255)
                                               tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACgg                                                                                                            <  2:144685/120‑1 (MQ=255)
                                               tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACgg                                                                                                            >  1:144685/1‑120 (MQ=255)
                                               tGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCACCa                                                                                        >  1:1034060/1‑140 (MQ=255)
                                                       gCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGAt                                                                                >  1:1088073/1‑140 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:315200/140‑1 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:1311391/140‑1 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:481729/140‑1 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:481844/140‑1 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:59530/140‑1 (MQ=255)
                                                          cGTGCGCCAGTAATTTTGTTAGTGGACGTCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTgg                                                                             <  1:313529/140‑1 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               >  2:1610106/1‑120 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               >  2:610572/1‑120 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               >  2:1610020/1‑120 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               <  1:610572/120‑1 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               <  1:1610020/120‑1 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTTCTGGATGTCTGGCTGGTGATGGGGCTGCATATTAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAata                                                                                               <  1:1610106/120‑1 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                                                           >  2:1417477/1‑140 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                                                           >  1:270767/1‑140 (MQ=255)
                                                            tGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTc                                                                           >  1:352612/1‑140 (MQ=255)
                                                              cgcCAGTAATTTTGTTGGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTg                                                                         >  2:1267259/1‑140 (MQ=255)
                                                              cgcCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTg                                                                         >  2:189219/1‑140 (MQ=255)
                                                                   gTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAAt                                                                    >  2:293649/1‑140 (MQ=255)
                                                                            ttAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTAGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTaa                                                           <  1:1591990/140‑1 (MQ=255)
                                                                              aGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTaaaa                                                         >  2:1570662/1‑140 (MQ=255)
                                                                                  gTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCt                                                     >  2:1032129/1‑140 (MQ=255)
                                                                                            tCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTAcgc                                           <  2:1291905/140‑1 (MQ=255)
                                                                                                      tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg                                 >  1:1520851/1‑140 (MQ=255)
                                                                                                      tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg                                 >  1:348426/1‑140 (MQ=255)
                                                                                                      tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg                                 >  1:34109/1‑140 (MQ=255)
                                                                                                      tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCGAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCg                                 >  1:1126341/1‑140 (MQ=255)
                                                                                                      tgtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGGCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATATCCg                                 >  1:192102/1‑140 (MQ=255)
                                                                                                       gtgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCgg                                >  1:1001307/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  2:842722/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  2:1653629/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  2:915588/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  1:1640099/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  1:847617/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  1:1640297/1‑140 (MQ=255)
                                                                                                        tgCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGc                               >  1:195734/1‑140 (MQ=255)
                                                                                                           tGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGaa                            <  2:1280417/140‑1 (MQ=255)
                                                                                                             gATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAAt                          >  1:626996/1‑140 (MQ=255)
                                                                                                             gATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAAt                          >  1:625855/1‑140 (MQ=255)
                                                                                                                   tggctggTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCCGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGaa                    <  2:128282/140‑1 (MQ=255)
                                                                                                                     gctggTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGaaaa                  <  1:1303606/140‑1 (MQ=255)
                                                                                                                     gctggTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGaaaa                  <  1:70778/140‑1 (MQ=255)
                                                                                                                     gctggTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGaaaa                  <  1:428734/140‑1 (MQ=255)
                                                                                                                     gatggTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGaaaa                  >  1:430454/3‑140 (MQ=255)
                                                                                                                              ggggCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGc         <  2:444561/140‑1 (MQ=255)
                                                                                                                               gggCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCg        <  1:581174/140‑1 (MQ=255)
                                                                                                                                   tGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGa    >  1:893291/1‑140 (MQ=255)
                                                                                                                                   tGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGa    >  1:99253/1‑140 (MQ=255)
                                                                                                                                     cATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGaaa  <  1:1210572/140‑1 (MQ=255)
                                                                                                                                     cATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGaaa  <  1:1210358/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                      
ACCGGCGTCGCTGGCGAATCTGGTATTACTCGGTTGGTTACTCGGCGTGCAATATGCCCGTGCGCCAGTAATTTTGTTAGTGGTCGGCAATATCCTCAACATTGTGCTGGATGTCTGGCTGGTGATGGGGCTGCATATGAACGTGCAGGGCGCGGCGCTGGCGACGGTTATTGCGGAATATGCAACATTGCTGATTGGTCTGCTAATGGTGCGTAAAATCCTCAAACTACGCGGAATTTCCGGCGAAATGCTGAAAACTGCCTGGCGAGGAAA  >  NC_000913/4258212‑4258484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: