Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 222,965 (A)8→7 coding (133/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913222,9580A.88.1% 307.4 / 43.7 84coding (126/576 nt)gmhBD,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (7/3);  new base . (33/41);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGAT  >  NC_000913/222822‑223093
                                                                                                                                        |                                                                                                                                       
aGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaa                                                                                                                                      >  1:863545/1‑140 (MQ=255)
aGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaa                                                                                                                                      >  2:178957/1‑140 (MQ=255)
    ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                  >  1:787102/1‑139 (MQ=255)
    ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                  >  1:1447429/1‑139 (MQ=255)
    ataAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                  <  2:30679/140‑2 (MQ=255)
    ataAAAGGGGGCGAAGAGCGTACCCGCAATTTTACTTGACCGTTATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACATCTTTGATTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaat                                                                                                                                  <  2:30359/140‑2 (MQ=255)
     taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaatg                                                                                                                                 >  1:369780/1‑138 (MQ=255)
         agttgtcGAAGAGCTTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCt                                                                                                                            <  1:563523/134‑1 (MQ=255)
          ggTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCtt                                                                                                                           >  1:26951/1‑140 (MQ=255)
            tGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTg                                                                                                                         >  1:1233878/1‑140 (MQ=255)
             ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgc                                                                                                                        >  1:257333/1‑140 (MQ=255)
              gctatgagCGAACCCGCAATTTTTCTTTACCGTTTTGGAACAATTATTTGGGATAACGGATTTGTCAATGTGATCTAAAAATTTTAATTTATCGACGGTTTTTTTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcg                                                                                                                       <  2:619583/135‑1 (MQ=255)
              gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcg                                                                                                                       <  1:1439097/140‑1 (MQ=255)
              gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcg                                                                                                                       <  1:1560549/140‑1 (MQ=255)
               cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTgcg                                                                                                                       >  2:1043622/1‑140 (MQ=255)
               cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcgc                                                                                                                      >  1:532995/1‑140 (MQ=255)
               cGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTgcgc                                                                                                                      >  2:68782/1‑140 (MQ=255)
                  agagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtgg                                                                                                                   <  1:533363/140‑1 (MQ=255)
                  agagCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtgg                                                                                                                   <  1:730144/140‑1 (MQ=255)
                     gCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCtggtgg                                                                                                                >  2:1288636/1‑140 (MQ=255)
                      cGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTggtggt                                                                                                               <  2:637368/140‑1 (MQ=255)
                              cAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGGTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTaacc                                                                                                        >  1:223755/1‑140 (MQ=255)
                                aTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCTCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaac                                                                                                     <  2:144695/140‑1 (MQ=255)
                                aTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaac                                                                                                     <  2:336646/140‑1 (MQ=255)
                                aTTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTaaccaac                                                                                                     <  2:422844/140‑1 (MQ=255)
                                  ttttCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAAATTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAaccaacca                                                                                                   <  2:924278/140‑1 (MQ=255)
                                          aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAACAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCa                                                                                           <  2:1117854/140‑1 (MQ=255)
                                          aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCa                                                                                           <  2:1117425/140‑1 (MQ=255)
                                           ccGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCTGTCTGGCAt                                                                                          >  2:856292/1‑140 (MQ=255)
                                                   gCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgc                                                                                   <  1:1032663/140‑1 (MQ=255)
                                                               tggttgaCGGCTATGTCCATGAGATCGACAACCTTGAATTGATCGACGGTGTTATTGCCGCCATGCGCGAGCTAAAAATATGGGCTCTGCGCTGGTGGTAGTAACCAACCTGTCGGGCATTGCTCGCGGTAAATTTACCg                                                                      <  2:1014282/134‑1 (MQ=255)
                                                               tCGATCCCGGCTATGTCCATGAGATCGACAACTTTGTCTTTATCGTCGGTGTTATTGCCGCCATGCGCGAGCTTTAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTGGCGGTAATTTTACCg                                                                      <  2:1013863/140‑1 (MQ=255)
                                                                 gATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGa                                                                     >  1:938068/1‑140 (MQ=255)
                                                                 gATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGa                                                                     >  1:57795/1‑140 (MQ=255)
                                                                 gATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGa                                                                     >  1:57930/1‑140 (MQ=255)
                                                                  aTCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAg                                                                   >  1:1041269/1‑140 (MQ=255)
                                                                       ggCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGcaca                                                               <  1:1340756/140‑1 (MQ=255)
                                                                         cTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGtt                                                            >  1:227578/1‑140 (MQ=255)
                                                                         cTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGtt                                                            >  1:358824/1‑140 (MQ=255)
                                                                         cTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGtt                                                            >  1:469230/1‑140 (MQ=255)
                                                                            tGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGa                                                         >  1:928941/1‑140 (MQ=255)
                                                                            tGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGa                                                         >  1:928953/1‑140 (MQ=255)
                                                                              tCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGaaa                                                       <  2:301989/140‑1 (MQ=255)
                                                                                cATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACg                                                     <  2:863545/140‑1 (MQ=255)
                                                                                 aTGAGATCGACAACTTTTAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACg                                                     >  1:894977/1‑140 (MQ=255)
                                                                                 aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGc                                                    >  2:37626/1‑140 (MQ=255)
                                                                                 aTGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGc                                                    >  2:284826/1‑140 (MQ=255)
                                                                                   gaggTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTg                                                  >  2:322374/1‑140 (MQ=255)
                                                                                   gagaTGGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATTCGCGTGCTAAAATAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGATACGCTg                                                  >  2:996825/1‑140 (MQ=255)
                                                                                   gagaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCt                                                   >  1:117384/1‑140 (MQ=255)
                                                                                   gagaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTg                                                  >  1:686875/1‑140 (MQ=255)
                                                                                       tagACAACTTTGACTTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAATAGGGGCTTGGCGCTGGTGGTAGTAACCAACCAGTCTGGAATTGCTCGCGTTAAATTTACCGAAGCACAGTTTGAAACGCTGACCg                                              <  2:218184/138‑1 (MQ=255)
                                                                                       tCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAACCAATGGGCTTTGCGCTGGTGGTAGTCACCAACCAGTCTGGCATTGCTCGCGGTAACTTTACCGAAGCACAGTTTGCAACGCTGACCg                                              <  2:207935/140‑1 (MQ=255)
                                                                                       tCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCg                                              <  2:1405298/140‑1 (MQ=255)
                                                                                       tCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCg                                              <  2:591264/140‑1 (MQ=255)
                                                                                        cGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGa                                             <  1:15841/140‑1 (MQ=255)
                                                                                        cGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGa                                             >  1:272002/1‑140 (MQ=255)
                                                                                        cGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGa                                             >  1:948198/1‑140 (MQ=255)
                                                                                             aCTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtgga                                        <  2:1545300/140‑1 (MQ=255)
                                                                                                 tGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTTCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgga                                    <  1:1252800/140‑1 (MQ=255)
                                                                                                 tGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatgga                                    <  2:1447429/140‑1 (MQ=255)
                                                                                                     tttATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTgg                                >  2:171552/1‑140 (MQ=255)
                                                                                                      ttATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGt                               >  2:1473064/1‑140 (MQ=255)
                                                                                                        aTCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCg                             <  2:928953/140‑1 (MQ=255)
                                                                                                        aTCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCg                             <  2:928941/140‑1 (MQ=255)
                                                                                                        aTCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCg                             >  1:441696/1‑140 (MQ=255)
                                                                                                        aTCGACGGTGTTATTGACGCCATGCGCGAGCT‑‑AAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGc                            >  1:1379317/1‑140 (MQ=255)
                                                                                                            aCGGTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTgg                         <  2:787102/140‑1 (MQ=255)
                                                                                                              ggTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGACACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                       <  2:697463/140‑1 (MQ=255)
                                                                                                              ggTGTTATTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                       <  1:441101/140‑1 (MQ=255)
                                                                                                                   tactGACGCCATGCGCGAGCAAAACAAATCGGCTTTGCGCTGGAGGTAGTAACCAACCAGTCTGGCAGTGCTCTCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCg                  <  2:1144315/137‑1 (MQ=255)
                                                                                                                    aTTGACGCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCga                 <  2:369780/140‑1 (MQ=255)
                                                                                                                          gCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc           >  1:1165498/1‑140 (MQ=255)
                                                                                                                          gCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc           >  1:1113378/1‑140 (MQ=255)
                                                                                                                          gCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc           >  1:414011/1‑140 (MQ=255)
                                                                                                                          gCCATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGGTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc           >  1:23619/1‑140 (MQ=255)
                                                                                                                            cATGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGa         >  1:196269/1‑140 (MQ=255)
                                                                                                                             ctGCGCGAGCTAAAACAATGGGCTTTGCGCTGGTGGTAGTACCCTACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGACACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAt        <  2:228290/139‑1 (MQ=255)
                                                                                                                             aTGCGCGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGACTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTATGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGAt        <  2:228308/140‑1 (MQ=255)
                                                                                                                              tGCGCGAGCTAAAAACATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATc       <  2:338355/140‑1 (MQ=255)
                                                                                                                               gcgcGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt      >  1:667422/1‑140 (MQ=255)
                                                                                                                               gcgcGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt      >  2:1204616/1‑140 (MQ=255)
                                                                                                                               gcgcGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCt      >  2:1204586/1‑140 (MQ=255)
                                                                                                                               gcgcGAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTATGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGATTACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATTTCGATCt      >  2:1204632/1‑140 (MQ=255)
                                                                                                                                  cgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACTCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa   <  1:1577611/140‑1 (MQ=255)
                                                                                                                                  cgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa   <  1:642421/140‑1 (MQ=255)
                                                                                                                                  cgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa   <  2:441696/140‑1 (MQ=255)
                                                                                                                                  cgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa   <  1:111597/140‑1 (MQ=255)
                                                                                                                                  cgAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGa   <  1:643510/140‑1 (MQ=255)
                                                                                                                                   gAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGAt  <  2:257333/140‑1 (MQ=255)
                                                                                                                                   gAGCTAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGAt  <  2:1165638/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGAT  >  NC_000913/222822‑223093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: