Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,096,379 C→T 5.5% A187V (GCG→GTG)  yggT → putative inner membrane protein; compensates for loss in K+ uptake

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,096,3790CT 5.5% 369.8 / 7.8 109A187V (GCG→GTG) yggTputative inner membrane protein; compensates for loss in K+ uptake
Reads supporting (aligned to +/- strand):  ref base C (29/73);  new base T (3/3);  total (33/76)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 109 total aligned reads displayed.

CTGCGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACGGCCAGGCCAA  >  NC_000913/3096231‑3096525
                                                                                                                                                    |                                                                                                                                                  
ctgcGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCg                                                                                                                                                   <  1:309241/150‑1 (MQ=255)
ctgcGCCCGAGTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCg                                                                                                                                                   <  1:309278/150‑1 (MQ=255)
 tgcGCCCGATTCGCCGCCTGCTACCGGCACTGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCACTATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGt                                                                                                                                                  <  1:1586470/150‑1 (MQ=255)
 tgcGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCATAAGTATTACAGGCAACCGGAAACATGCTGCTGCCAGGGCTGTGGATGGCGt                                                                                                                                                  <  1:1422426/150‑1 (MQ=255)
 tgcGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGt                                                                                                                                                  <  2:503997/150‑1 (MQ=255)
 tgcGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGt                                                                                                                                                  >  1:735647/1‑150 (MQ=255)
 tgcGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAACATGCTGCTGCCAGGGCTGTGGATGGCGt                                                                                                                                                  <  1:1422478/150‑1 (MQ=255)
   cgcCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTGCAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTa                                                                                                                                                <  2:172361/150‑1 (MQ=255)
     cccGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATg                                                                                                                                              >  1:1455891/1‑150 (MQ=255)
      ccGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGa                                                                                                                                             >  1:559706/1‑150 (MQ=255)
        gATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCACCCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAAt                                                                                                                                           >  2:355968/1‑150 (MQ=255)
        gATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAAt                                                                                                                                           >  2:1518196/1‑150 (MQ=255)
          ttCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGc                                                                                                                                         <  2:134925/150‑1 (MQ=255)
          ttCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGc                                                                                                                                         <  2:187194/150‑1 (MQ=255)
          ttCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGc                                                                                                                                         <  2:187164/150‑1 (MQ=255)
          ttCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGc                                                                                                                                         <  2:374525/150‑1 (MQ=255)
            cgccgcCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCg                                                                                                                                       <  2:1424149/150‑1 (MQ=255)
            cgccgcCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCg                                                                                                                                       <  2:1424164/150‑1 (MQ=255)
            cgccgcCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCg                                                                                                                                       <  2:1424162/150‑1 (MQ=255)
                gccTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAAc                                                                                                                                   <  2:1116507/150‑1 (MQ=255)
                gccTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAAc                                                                                                                                   <  2:1451345/150‑1 (MQ=255)
                  cTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAg                                                                                                                                 <  2:1111888/150‑1 (MQ=255)
                   tgcgtccGGCTATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATACGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGt                                                                                                                                <  2:931575/145‑1 (MQ=255)
                   tGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCTTCCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGt                                                                                                                                <  2:931549/150‑1 (MQ=255)
                   tGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGTGTTATGAATGCCGTAACAGt                                                                                                                                >  1:64743/1‑150 (MQ=255)
                   tGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGTGTTATGAATGCCGTAACAGt                                                                                                                                >  1:1267839/1‑150 (MQ=255)
                   tGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGTGTTATGAATGCCGTAACAGt                                                                                                                                >  1:1267829/1‑150 (MQ=255)
                      tACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTaa                                                                                                                             <  2:1294755/150‑1 (MQ=255)
                      tACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTaa                                                                                                                             <  2:1294720/150‑1 (MQ=255)
                      tACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTaa                                                                                                                             <  2:1294653/150‑1 (MQ=255)
                       aCCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAat                                                                                                                            <  1:795529/150‑1 (MQ=255)
                        ccGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAatg                                                                                                                           >  2:559470/1‑150 (MQ=255)
                        ccGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAatg                                                                                                                           >  2:1338160/1‑150 (MQ=255)
                        ccGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAatg                                                                                                                           >  1:577302/1‑150 (MQ=255)
                            cAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAAtgatga                                                                                                                       <  2:1330240/150‑1 (MQ=255)
                                 ggtgggATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTc                                                                                                                  <  1:1680312/150‑1 (MQ=255)
                                     gtattgattTCTCGCCGATGATCCTCGTTCTGCTGCTTTATTTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATTCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCTTAACATTTAATGATGACTGTCTgtt                                                                                                              <  2:1007510/148‑3 (MQ=255)
                                        ttgattTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGtttt                                                                                                           >  2:1055673/1‑150 (MQ=255)
                                         tgattTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTa                                                                                                          <  1:273695/150‑1 (MQ=255)
                                            tttCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCTATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGTTGGCGTTATGAATGCCGTAACAGTTAATGATGACTGTCTGGTTTTACgg                                                                                                       <  2:1095876/150‑1 (MQ=255)
                                            tttCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACgg                                                                                                       <  2:782002/150‑1 (MQ=255)
                                             ttCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGc                                                                                                      <  1:1585194/150‑1 (MQ=255)
                                                   ccGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCtata                                                                                                >  2:1297485/1‑150 (MQ=255)
                                                   ccGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCtata                                                                                                >  2:1297503/1‑150 (MQ=255)
                                                   ccGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGAGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCtata                                                                                                >  2:213650/1‑150 (MQ=255)
                                                       tgatCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCa                                                                                            <  2:629317/150‑1 (MQ=255)
                                                       tgatCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCa                                                                                            <  2:300008/150‑1 (MQ=255)
                                                           ccTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCg                                                                                        >  2:180893/1‑150 (MQ=255)
                                                             tCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGaa                                                                                      >  1:20697/1‑150 (MQ=255)
                                                             tCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGaa                                                                                      <  1:839391/150‑1 (MQ=255)
                                                                ttCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAagc                                                                                   <  2:1178586/150‑1 (MQ=255)
                                                                  ctgctgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAagcca                                                                                 <  2:1238797/150‑1 (MQ=255)
                                                                  ctgctgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAagcca                                                                                 <  1:38512/150‑1 (MQ=255)
                                                                  ctgctgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAagcca                                                                                 <  2:637136/150‑1 (MQ=255)
                                                                     ctgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAagccagcc                                                                              >  2:1343432/1‑150 (MQ=255)
                                                                      tgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCg                                                                             <  1:602260/150‑1 (MQ=255)
                                                                      tgctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCg                                                                             <  2:627788/150‑1 (MQ=255)
                                                                       gctgTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGt                                                                            <  2:1271066/150‑1 (MQ=255)
                                                                           tATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGAtt                                                                        <  1:123442/150‑1 (MQ=255)
                                                                                  tcaATATGGGTGACGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGt                                                                 >  1:717876/1‑150 (MQ=255)
                                                                                      tatGTGTGTCTAAGAAGTATTAAAGTCAACCTTAAATATTCTTCTTCAAGTTCTGTGGATGGCGTTATGAATGCCTTAACATTTAATGATGAAGTTCTTGTTTTACGTATCTATATTCAGCCGAAAGCAATACGTGATTCTATTTTCgtt                                                             <  2:1010744/150‑3 (MQ=255)
                                                                                      tatGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGt                                                             <  2:1490007/150‑1 (MQ=255)
                                                                                         gggTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTa                                                          <  1:559470/150‑1 (MQ=255)
                                                                                           gtgtCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACa                                                        >  2:1129662/1‑150 (MQ=255)
                                                                                              tCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATgg                                                     <  1:303091/150‑1 (MQ=255)
                                                                                              tCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATgg                                                     <  2:167720/150‑1 (MQ=255)
                                                                                                gCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGcg                                                   >  2:310966/1‑150 (MQ=255)
                                                                                                 cAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGcga                                                  <  2:846127/150‑1 (MQ=255)
                                                                                                  aGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGcgac                                                 >  1:1034617/1‑150 (MQ=255)
                                                                                                  aGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGATGTGGATGACGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGcgac                                                 >  1:240923/1‑150 (MQ=255)
                                                                                                       tATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGt                                            <  2:20697/150‑1 (MQ=255)
                                                                                                       tATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGAGTCTATTGTCGGTTTACATGGCGACGAAGt                                            >  2:1540815/1‑150 (MQ=255)
                                                                                                         ttACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTa                                          >  1:205223/1‑150 (MQ=255)
                                                                                                         ttACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTa                                          >  1:205211/1‑150 (MQ=255)
                                                                                                           aCAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTaaa                                        <  2:375699/150‑1 (MQ=255)
                                                                                                               gCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCg                                    >  2:1588639/1‑150 (MQ=255)
                                                                                                               gCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCg                                    >  2:794616/1‑150 (MQ=255)
                                                                                                                cAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGc                                   <  2:926485/150‑1 (MQ=255)
                                                                                                                      gAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTAc                             >  2:206883/1‑150 (MQ=255)
                                                                                                                      gAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTAc                             >  1:443746/1‑150 (MQ=255)
                                                                                                                        aaTATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACcg                           <  1:1113388/150‑1 (MQ=255)
                                                                                                                         atatGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACcgc                          <  2:962847/150‑1 (MQ=255)
                                                                                                                         atatGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACcgc                          <  1:160481/150‑1 (MQ=255)
                                                                                                                         atatGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACcgc                          <  1:160570/150‑1 (MQ=255)
                                                                                                                         atatGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACcgc                          <  2:1392920/150‑1 (MQ=255)
                                                                                                                               tgctgcCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCgccgcc                    >  2:922775/1‑150 (MQ=255)
                                                                                                                                   gcCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGtt                <  1:95762/150‑1 (MQ=255)
                                                                                                                                     cAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGa              <  2:1047180/150‑1 (MQ=255)
                                                                                                                                     cAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGa              <  2:735647/150‑1 (MQ=255)
                                                                                                                                       gggCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACg            <  2:860912/150‑1 (MQ=255)
                                                                                                                                       gggCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACg            <  2:578824/150‑1 (MQ=255)
                                                                                                                                       gggCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACg            <  2:578713/150‑1 (MQ=255)
                                                                                                                                           tgtgGATGGTGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggcca        <  2:1267829/150‑1 (MQ=255)
                                                                                                                                           tgtgGATGGTGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggcca        <  2:64743/150‑1 (MQ=255)
                                                                                                                                           tgtgGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggcca        >  1:338562/1‑150 (MQ=255)
                                                                                                                                              tgATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTATTGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggacaggc     <  2:794743/149‑1 (MQ=255)
                                                                                                                                              ggATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggccaggc     <  2:559348/150‑1 (MQ=255)
                                                                                                                                              ggATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACggccaggc     <  2:794723/150‑1 (MQ=255)
                                                                                                                                                 tGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACGGCCAGGCCaa  <  2:1521054/150‑1 (MQ=255)
                                                                                                                                                 tGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACGGCCAGGCCaa  <  2:1521202/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGCGCCCGATTCGCCGCCTGCTACCGGCAATGGGTGGGATTGATTTCTCGCCGATGATCCTCGTTCTGCTGCTGTATGTCATCAATATGGGTGTCGCAGAAGTATTACAGGCAACCGGAAATATGCTGCTGCCAGGGCTGTGGATGGCGTTATGAATGCCGTAACAGTTAATGATGACGGTCTGGTTTTACGGCTCTATATTCAGCCGAAAGCCAGCCGTGATTCTATTGTCGGTTTACATGGCGACGAAGTTAAAGTCGCCATTACCGCGCCGCCGGTTGACGGCCAGGCCAA  >  NC_000913/3096231‑3096525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: