Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,437,910 A→T 5.3% intergenic (+16/‑114) trkA → / → mscL NAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,437,9100AT 5.3% 330.8 / 7.8 94intergenic (+16/‑114)trkA/mscLNAD‑binding component of TrK potassium transporter/mechanosensitive channel protein, high conductance
Reads supporting (aligned to +/- strand):  ref base A (41/48);  new base T (3/2);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437761‑3438058
                                                                                                                                                     |                                                                                                                                                    
gTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTaa                                                                                                                                                      >  2:58222/1‑150 (MQ=255)
   gAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGa                                                                                                                                                   >  2:1193446/1‑150 (MQ=255)
       cgacgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGcc                                                                                                                                               <  2:1284782/150‑1 (MQ=255)
         acgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCtt                                                                                                                                             <  2:716504/150‑1 (MQ=255)
          cgTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCtaa                                                                                                                                            <  1:9117/150‑3 (MQ=255)
            tgatgaTTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCttatt                                                                                                                                          <  2:1331077/150‑1 (MQ=255)
                  ttGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTcc                                                                                                                                    <  2:726590/150‑1 (MQ=255)
                   tGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCATTAGACGCCTTATTAattaca                                                                                                                                   <  1:473414/150‑4 (MQ=255)
                    gCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCt                                                                                                                                  >  1:684530/1‑150 (MQ=255)
                     ccAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCtt                                                                                                                                 >  1:1043999/1‑150 (MQ=255)
                      cAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGACAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCttt                                                                                                                                <  2:1692453/150‑1 (MQ=255)
                       aaTGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAGAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTg                                                                                                                               >  2:1448679/1‑150 (MQ=255)
                              aTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCa                                                                                                                        >  1:744581/1‑150 (MQ=255)
                              aTCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCa                                                                                                                        >  1:744563/1‑150 (MQ=255)
                               tCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCaa                                                                                                                       <  1:1354509/150‑1 (MQ=255)
                                cTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAg                                                                                                                      <  1:1189679/150‑1 (MQ=255)
                                 tGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAgg                                                                                                                     >  1:1252901/1‑150 (MQ=255)
                                    gcATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTATTTTAATTAATAAGGCGTCTAATGAAGCCTTATTATTTCCCTTTGATTATCaattatt                                                                                                                  >  2:1594062/1‑145 (MQ=255)
                                     cATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                 <  2:490225/150‑1 (MQ=255)
                                     cATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                 <  2:1329063/150‑1 (MQ=255)
                                     cATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGatta                                                                                                                 <  2:766613/150‑1 (MQ=255)
                                      aTTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaa                                                                                                                <  2:1255193/150‑1 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               <  1:589266/150‑1 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               >  2:786304/1‑150 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               <  1:125767/150‑1 (MQ=255)
                                       ttGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaat                                                                                                               >  2:1534383/1‑150 (MQ=255)
                                         gAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGattaatta                                                                                                             <  1:1115579/150‑1 (MQ=255)
                                            cAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTATTTAAat                                                                                                          >  2:1150430/1‑150 (MQ=255)
                                            cAAGGCGATCACGTAAATATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTGTGATTATCAAGGATTAATTAAat                                                                                                          >  2:566346/1‑150 (MQ=255)
                                               ggCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattca                                                                                                       <  2:299961/150‑1 (MQ=255)
                                                  gATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAattcattc                                                                                                    <  2:298498/150‑1 (MQ=255)
                                                    tCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCt                                                                                                  <  1:363390/150‑1 (MQ=255)
                                                    tCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCt                                                                                                  <  1:1454080/150‑1 (MQ=255)
                                                    tCACGTAATTATGGTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCt                                                                                                  <  1:647557/150‑1 (MQ=255)
                                                        gTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCa                                                                                              >  2:583015/1‑150 (MQ=255)
                                                         tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAg                                                                                             >  1:92277/1‑150 (MQ=255)
                                                         tAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTAGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCACTGTGATTATCAAGGATTAATTAAATTCATTCCTGGAAg                                                                                             >  2:1616393/1‑150 (MQ=255)
                                                              aTGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGaaaa                                                                                        <  2:696210/150‑1 (MQ=255)
                                                               tGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAAt                                                                                       <  2:1252901/150‑1 (MQ=255)
                                                               tGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAAt                                                                                       <  1:1534383/150‑1 (MQ=255)
                                                                 ttCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATgg                                                                                     <  2:1308983/150‑1 (MQ=255)
                                                                  tCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGc                                                                                    <  2:919350/150‑1 (MQ=255)
                                                                     tCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTa                                                                                 >  1:1408090/1‑150 (MQ=255)
                                                                      cacaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTaa                                                                                <  2:684530/150‑1 (MQ=255)
                                                                       acaGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAAc                                                                               >  1:900583/1‑150 (MQ=255)
                                                                        caGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACa                                                                              <  1:1074407/150‑1 (MQ=255)
                                                                           aTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAttt                                                                           <  1:1461161/150‑1 (MQ=255)
                                                                           aTAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAttt                                                                           <  1:441103/150‑1 (MQ=255)
                                                                             aaaaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGt                                                                         <  2:692114/150‑1 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                       <  2:223799/150‑1 (MQ=255)
                                                                               aaaGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTa                                                                       <  2:1201000/150‑1 (MQ=255)
                                                                                  gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGAc                                                                    >  2:1109934/1‑150 (MQ=255)
                                                                                  gTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACAGTTGTTAGAc                                                                    >  2:1503262/1‑150 (MQ=255)
                                                                                           cGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTCGACTTAGGGTTg                                                           >  2:1660488/1‑150 (MQ=255)
                                                                                           cGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTg                                                           >  1:1336309/1‑150 (MQ=255)
                                                                                           cGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTg                                                           >  2:1507941/1‑150 (MQ=255)
                                                                                           cGACGTCGAAAGACTCTACCAGCCAAGCCCTTTCTTCTTGTAATTAAAAAGGCGTCTAAAGACGCCTTATTATTTCCCGGGGATTATCAAGGATTAATTAAATTCAATCCTGGCAGGACCATGGCTTAACAGTTGGTAGACTTATGGTTg                                                           >  1:1542108/1‑150 (MQ=255)
                                                                                                  gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAATTTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                    >  2:266656/1‑150 (MQ=255)
                                                                                                  gAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCtt                                                    <  2:901044/150‑1 (MQ=255)
                                                                                                   aaaGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTc                                                   >  2:948926/1‑150 (MQ=255)
                                                                                                      gACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATa                                                <  1:1448679/150‑1 (MQ=255)
                                                                                                                gacAAGACCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAATGATTAATTAAATTCATTCCTTGCAGGATAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaa                                      <  1:1510883/148‑1 (MQ=255)
                                                                                                                gCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaa                                      >  1:1390901/1‑150 (MQ=255)
                                                                                                                gCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATaa                                      <  1:1510896/150‑1 (MQ=255)
                                                                                                                     gCCCTTTCTTCTTGTAATTAATTAGGCGTATAATGACGCATTATTATTTCCCTTTGATTATAAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGTTTGTCGGCTTCATAGGGAGAATAACATGa                                 <  2:1408481/150‑1 (MQ=255)
                                                                                                                     gCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                                 <  1:858223/150‑1 (MQ=255)
                                                                                                                     gCCCTTTCTTATTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGa                                 <  2:425456/150‑1 (MQ=255)
                                                                                                                       ccTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGc                               <  1:1193446/150‑1 (MQ=255)
                                                                                                                         tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCat                             >  1:768842/1‑150 (MQ=255)
                                                                                                                         tttCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCat                             >  1:768909/1‑150 (MQ=255)
                                                                                                                           tcttctTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCatga                           >  1:1576505/1‑148 (MQ=255)
                                                                                                                             ttcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCattatt                         <  1:583015/150‑1 (MQ=255)
                                                                                                                              tcttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCAttatta                        >  2:200071/1‑150 (MQ=255)
                                                                                                                                ttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTACTTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                      <  2:852454/150‑1 (MQ=255)
                                                                                                                                ttGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTaaa                      >  1:1249603/1‑150 (MQ=255)
                                                                                                                                   taattaatAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGaa                   <  1:270936/150‑1 (MQ=255)
                                                                                                                                   gaaataataaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTCACAGTTGTTAGACTTATGGTTGTCGGCTTCAGAGGGAGACTAACCTGAGCCTGATTCAAGaa                   >  1:1163592/5‑150 (MQ=255)
                                                                                                                                        aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg              >  1:164360/1‑150 (MQ=255)
                                                                                                                                        aataaGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcg              >  1:164272/1‑150 (MQ=255)
                                                                                                                                          taaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg            >  2:273943/1‑150 (MQ=255)
                                                                                                                                          taaGGCGTCATTAGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTcgcg            >  2:1057623/1‑150 (MQ=255)
                                                                                                                                              gCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAtt        >  2:585265/1‑150 (MQ=255)
                                                                                                                                              gCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAtt        >  2:961899/1‑150 (MQ=255)
                                                                                                                                               cGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAAttt       >  2:1184387/1‑150 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      >  2:361442/1‑150 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      >  2:1577245/1‑150 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  2:1567408/150‑1 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  1:850904/150‑1 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  1:678004/150‑1 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  1:304360/150‑1 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  1:304357/150‑1 (MQ=255)
                                                                                                                                                gTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTg      <  1:1285560/150‑1 (MQ=255)
                                                                                                                                                 tCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGc     >  2:381003/1‑150 (MQ=255)
                                                                                                                                                 tCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGACTAACATGAGCATTATTAAAGAATTTCGCGAATTTGct    >  2:1359471/1‑149 (MQ=255)
                                                                                                                                                   tAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGa   >  2:1128990/1‑150 (MQ=255)
                                                                                                                                                    aaTGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAt  >  2:1349198/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTGGAAACGACGTGATGATTGCCAATGACAATCTGCGCATTGAGCAAGGCGATCACGTAATTATGTTCCTCACAGATAAAAAGTTTATTACCGACGTCGAAAGACTCTTCCAGCCAAGCCCTTTCTTCTTGTAATTAATAAGGCGTCTAATGACGCCTTATTATTTCCCTTTGATTATCAAGGATTAATTAAATTCATTCCTGGCAGGAAAATGGCTTAACATTTGTTAGACTTATGGTTGTCGGCTTCATAGGGAGAATAACATGAGCATTATTAAAGAATTTCGCGAATTTGCGAT  >  NC_000913/3437761‑3438058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: