Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,439,963 T→C 7.7% N13D (AAC→GAC)  rplQ ← 50S ribosomal subunit protein L17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,439,9630TC 7.7% 407.0 / 18.6 129N13D (AAC→GAC) rplQ50S ribosomal subunit protein L17
Reads supporting (aligned to +/- strand):  ref base T (63/56);  new base C (5/5);  total (68/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 132 total aligned reads displayed.

ATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGC  >  NC_000913/3439814‑3440112
                                                                                                                                                     |                                                                                                                                                     
atgaGCAACGCTATCAGTCTTGGCACGAGTACTCAGCGGCTCACCTACGCGGCGCAGCTCTTTCGCGTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGACCCTGCCATATTGCGGAACCTAGCCTGGCGATGGCTGCTGtt                                                                                                                                                       <  2:380199/147‑1 (MQ=255)
 ttAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTg                                                                                                                                                      <  2:976825/150‑1 (MQ=255)
    gCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCgg                                                                                                                                                   >  2:544960/1‑150 (MQ=255)
       aCGCTATCAGTCTTGGCAAGAGAAATCAGCGGCTCAACTACGCGGCGCAGCTCATTCGCTTTAGGCATAGTCGTCTTGATGATTTCATGACGAACCACTGAACCTGCCATATTGCGGAACAAAGCCAGGCGAGGGCTGCTGTTGCGGTTc                                                                                                                                                >  2:1061191/1‑150 (MQ=255)
         gCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAg                                                                                                                                              >  1:639681/1‑150 (MQ=255)
             tCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTga                                                                                                                                          <  2:141816/150‑1 (MQ=255)
              cAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTgac                                                                                                                                         <  1:1351334/150‑1 (MQ=255)
              cAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTgac                                                                                                                                         <  1:1301853/150‑1 (MQ=255)
               aGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCAGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTgacg                                                                                                                                        >  1:1146957/1‑150 (MQ=255)
                 tCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTgacgac                                                                                                                                      <  1:1493867/150‑1 (MQ=255)
                 tCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTACCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTgacgac                                                                                                                                      >  1:720463/1‑150 (MQ=255)
                     ggCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCAct                                                                                                                                  <  1:1681687/150‑1 (MQ=255)
                       cAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCActct                                                                                                                                >  1:1582297/1‑150 (MQ=255)
                       cAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCActct                                                                                                                                <  2:517483/150‑1 (MQ=255)
                            gTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGa                                                                                                                           <  1:1674700/150‑1 (MQ=255)
                                 cAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGgcg                                                                                                                      <  2:507761/150‑1 (MQ=255)
                                  aGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGgcgc                                                                                                                     >  2:528153/1‑150 (MQ=255)
                                  aGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGgcgc                                                                                                                     >  2:883931/1‑150 (MQ=255)
                                      gCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGa                                                                                                                 <  2:817668/150‑1 (MQ=255)
                                       cTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGGTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGAc                                                                                                                >  2:1567229/1‑150 (MQ=255)
                                        tCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGAcc                                                                                                               <  1:10838/150‑1 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTGTTGATGATTTAATGACGAACCAGTGAACCTGCCATATTGGGGAACATAGCCTGGCGATGGCTGGTGTTGCGGTTCAGTTTTCGACCACTCTTAAGATTGCGCATgtactt                                                                                                             >  1:1536755/1‑145 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCtt                                                                                                             >  1:382246/1‑150 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCtt                                                                                                             >  2:155474/1‑150 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCtt                                                                                                             <  2:822532/150‑1 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCtt                                                                                                             >  1:258742/1‑150 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCAGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCATACGATGGCGCATGACCtt                                                                                                             >  1:1580574/1‑150 (MQ=255)
                                          aaCTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGAAGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCtt                                                                                                             >  1:382240/1‑150 (MQ=255)
                                             tACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATc                                                                                                          <  2:1377875/150‑1 (MQ=255)
                                             tACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATc                                                                                                          <  2:1377739/150‑1 (MQ=255)
                                               cgcgGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGTTGATTTCATGAAGAACCAGTGAACCTGCCATATTTCGGAACATAGCCAGGCGATGGCTGCTGTTGCGGTTCAGTTGAAGACCACGCTTACGATGGCGCATGAACTTATccg                                                                                                        >  1:840500/1‑149 (MQ=255)
                                                gcggcgCAGCTCATTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGCACCTGCCATATTGCGGAACAGAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCtt                                                                                                       <  1:818368/150‑1 (MQ=255)
                                                 cggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTtc                                                                                                      >  2:1124378/1‑150 (MQ=255)
                                                 cggcgCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTtc                                                                                                      <  1:1166563/150‑1 (MQ=255)
                                                   gcgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTtctc                                                                                                    <  2:1010033/150‑1 (MQ=255)
                                                    cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                   <  1:1424068/150‑1 (MQ=255)
                                                    cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                   <  1:1178174/150‑1 (MQ=255)
                                                    cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                   <  2:443587/150‑1 (MQ=255)
                                                    cgcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCa                                                                                                   <  1:105082/150‑1 (MQ=255)
                                                     gcAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAg                                                                                                  >  2:951245/1‑150 (MQ=255)
                                                      cAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGt                                                                                                 >  2:114318/1‑150 (MQ=255)
                                                         ctctTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTaaa                                                                                              >  2:1139517/1‑150 (MQ=255)
                                                            tttCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAAcc                                                                                           >  2:926156/1‑150 (MQ=255)
                                                            tttCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAAcc                                                                                           >  2:926150/1‑150 (MQ=255)
                                                              tCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCtt                                                                                         <  1:1048040/150‑1 (MQ=255)
                                                                 cTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                      >  2:1630912/1‑150 (MQ=255)
                                                                 cTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                      >  2:1630909/1‑150 (MQ=255)
                                                                 cTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAc                                                                                      >  2:1630900/1‑150 (MQ=255)
                                                                  tttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGTCATATTGCGGAACATAGCCTGGCGATGGCAGCTGTTGCGGTTAAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAcc                                                                                     >  2:816160/1‑150 (MQ=255)
                                                                  tttAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAAcc                                                                                     >  2:608617/1‑150 (MQ=255)
                                                                        cAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGAt                                                                               >  2:800390/1‑150 (MQ=255)
                                                                          gagTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACAAAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCAGTGATcc                                                                             >  2:517541/1‑150 (MQ=255)
                                                                                  ttGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTc                                                                     <  2:1277364/150‑1 (MQ=255)
                                                                                    gatgatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGt                                                                   >  1:281871/1‑150 (MQ=255)
                                                                                      tgatTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCa                                                                 >  2:950445/1‑150 (MQ=255)
                                                                                      tgatTTCAGGACGAACCAGTGAACCTGCCAGAGTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCGTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGGTACTCGTCa                                                                 <  2:583615/150‑1 (MQ=255)
                                                                                            cATGACGAACCAGTGAACCTGCCAGATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTCTCCTTCTCAGTCAAACCTTAACCTGTGATCCGGGTACTCGTCAGCGATg                                                           >  2:971017/1‑150 (MQ=255)
                                                                                             aTGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGc                                                          <  1:1376629/150‑1 (MQ=255)
                                                                                                 cGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGc                                                      <  2:516981/150‑1 (MQ=255)
                                                                                                  gAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGcc                                                     >  1:1430444/1‑150 (MQ=255)
                                                                                                     ccAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGt                                                  <  2:89987/150‑1 (MQ=255)
                                                                                                     ccAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGt                                                  <  2:935483/150‑1 (MQ=255)
                                                                                                      cAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTg                                                 >  1:1498/1‑150 (MQ=255)
                                                                                                        gTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGc                                               >  2:508375/1‑150 (MQ=255)
                                                                                                        gTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGc                                               >  1:1014359/1‑150 (MQ=255)
                                                                                                        gTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGc                                               >  1:1487098/1‑150 (MQ=255)
                                                                                                            aCCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGt                                           >  1:888696/1‑150 (MQ=255)
                                                                                                               tGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                        >  1:1253890/1‑150 (MQ=255)
                                                                                                               tGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                        >  1:531314/1‑150 (MQ=255)
                                                                                                               tGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGCTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGtttt                                        >  1:1160857/1‑150 (MQ=255)
                                                                                                                gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTc                                       <  2:792675/150‑1 (MQ=255)
                                                                                                                gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTc                                       <  2:419109/150‑1 (MQ=255)
                                                                                                                gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTc                                       <  2:122605/150‑1 (MQ=255)
                                                                                                                gCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTc                                       <  1:839370/150‑1 (MQ=255)
                                                                                                                  cATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCa                                     <  1:1071065/150‑1 (MQ=255)
                                                                                                                    tatTGCGGAGCATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAgg                                   >  1:1576400/1‑150 (MQ=255)
                                                                                                                    tatTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAgg                                   <  1:354335/150‑1 (MQ=255)
                                                                                                                        gCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCa                               >  2:856750/1‑150 (MQ=255)
                                                                                                                         cGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCAt                              >  1:1144471/1‑150 (MQ=255)
                                                                                                                          ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                             >  1:1387089/1‑150 (MQ=255)
                                                                                                                          ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                             >  1:1716657/1‑150 (MQ=255)
                                                                                                                          ggAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATg                             >  1:1387085/1‑150 (MQ=255)
                                                                                                                           gAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGc                            <  2:1649667/150‑1 (MQ=255)
                                                                                                                            aaCATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGcc                           >  2:789804/1‑150 (MQ=255)
                                                                                                                               aTAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCag                        <  2:1224468/150‑1 (MQ=255)
                                                                                                                                tAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGATGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCaga                       <  1:1100130/150‑1 (MQ=255)
                                                                                                                                 aGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCagag                      >  2:96632/1‑150 (MQ=255)
                                                                                                                                    cTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACa                   <  2:908584/150‑1 (MQ=255)
                                                                                                                                      ggCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGt                 <  1:902307/150‑1 (MQ=255)
                                                                                                                                      ggCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGt                 <  1:1023564/150‑1 (MQ=255)
                                                                                                                                               tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        <  2:281871/150‑1 (MQ=255)
                                                                                                                                               tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        >  2:1712256/1‑150 (MQ=255)
                                                                                                                                               tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        >  1:108454/1‑150 (MQ=255)
                                                                                                                                               tgctgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        >  1:265621/1‑150 (MQ=255)
                                                                                                                                               tgctgTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        <  1:1630894/150‑1 (MQ=255)
                                                                                                                                               tgctgTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        <  1:1630909/150‑1 (MQ=255)
                                                                                                                                               tgctgTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        <  1:1630912/150‑1 (MQ=255)
                                                                                                                                               tgctgTCGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGaa        <  1:1631003/150‑1 (MQ=255)
                                                                                                                                                  tgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGcc     >  2:1574521/1‑150 (MQ=255)
                                                                                                                                                     tGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGAGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGc  >  2:381842/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATTAGCAACGCTATCAGTCTTGGCAAGAGTAATCAGCGGCTCAACTACGCGGCGCAGCTCTTTCGCTTTAGGCAGAGTCGTCTTGATGATTTCATGACGAACCAGTGAACCTGCCATATTGCGGAACATAGCCTGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGC  >  NC_000913/3439814‑3440112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: