Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,710,531 C→T 5.3% intergenic (‑56/+268) eptB ← / ← yhjX KDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,710,5310CT 5.3% 261.3 / 3.1 76intergenic (‑56/+268)eptB/yhjXKDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter
Reads supporting (aligned to +/- strand):  ref base C (33/39);  new base T (2/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCGGTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTA  >  NC_000913/3710383‑3710680
                                                                                                                                                    |                                                                                                                                                     
gcggTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGccc                                                                                                                                                      >  2:648557/1‑150 (MQ=255)
 cggTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCg                                                                                                                                                     <  1:1660604/150‑1 (MQ=255)
  ggTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCgg                                                                                                                                                    <  1:997141/150‑1 (MQ=255)
  ggTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCgg                                                                                                                                                    <  1:997144/150‑1 (MQ=255)
    tAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCa                                                                                                                                                  >  1:1087308/1‑150 (MQ=255)
      aaaaaCCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAgg                                                                                                                                                <  1:1537442/150‑1 (MQ=255)
        aaaCCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGa                                                                                                                                              <  2:885886/150‑1 (MQ=255)
        aaaCCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGa                                                                                                                                              >  2:53295/1‑150 (MQ=255)
            cgcgCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGaaaaa                                                                                                                                          <  2:1057624/150‑1 (MQ=255)
            cgcgCCATTCATAAAAAGGCCAAGCGAGATGGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGaaaaa                                                                                                                                          <  2:191856/150‑1 (MQ=255)
              cgcCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGt                                                                                                                                        <  1:141683/150‑1 (MQ=255)
                    tCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGATTAGAGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAg                                                                                                                                  >  2:1678790/1‑150 (MQ=255)
                      aTAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGt                                                                                                                                <  1:853502/150‑1 (MQ=255)
                         aaaaGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTCATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCCGGGACCAAGTGCGGAGGTAGt                                                                                                                             >  2:466134/1‑150 (MQ=255)
                              gCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGAt                                                                                                                        >  2:1034821/1‑150 (MQ=255)
                               ccAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATa                                                                                                                       <  1:1409206/150‑1 (MQ=255)
                               ccAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATa                                                                                                                       <  1:965963/150‑1 (MQ=255)
                                cAATATAGAGTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATaa                                                                                                                      <  2:1514473/150‑1 (MQ=255)
                                    ataGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaa                                                                                                                  <  1:1629074/150‑1 (MQ=255)
                                    ataGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaa                                                                                                                  >  1:1533020/1‑150 (MQ=255)
                                    ataGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaa                                                                                                                  >  1:964429/1‑150 (MQ=255)
                                     taGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaaa                                                                                                                 <  1:351817/150‑1 (MQ=255)
                                     taGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaaa                                                                                                                 <  2:1087308/150‑1 (MQ=255)
                                      aGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCTCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaaaa                                                                                                                >  2:387117/1‑150 (MQ=255)
                                      aGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCTCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaaaa                                                                                                                >  2:387120/1‑150 (MQ=255)
                                      aGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTaaaa                                                                                                                >  2:968771/1‑150 (MQ=255)
                                       gATTGCAAGCAAAACGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTTGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAAt                                                                                                               <  1:1589208/150‑1 (MQ=255)
                                         ttGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCt                                                                                                             <  1:70930/150‑1 (MQ=255)
                                           gcaagcaaAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTac                                                                                                           <  1:41358/150‑1 (MQ=255)
                                              agcaaAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACAcg                                                                                                        <  2:837118/150‑1 (MQ=255)
                                              agcaaAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACAcg                                                                                                        >  2:1648826/1‑150 (MQ=255)
                                               gcaaAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACAcgc                                                                                                       <  1:1346399/150‑1 (MQ=255)
                                                    aaGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGaa                                                                                                  <  1:695706/150‑1 (MQ=255)
                                                       cTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGACTaa                                                                                               >  2:80597/1‑150 (MQ=255)
                                                       cTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATaa                                                                                               >  2:630303/1‑150 (MQ=255)
                                                        tCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAg                                                                                              <  2:616742/150‑1 (MQ=255)
                                                                     tgtAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCaa                                                                                 >  2:1079718/1‑150 (MQ=255)
                                                                       tAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAg                                                                               >  2:569310/1‑150 (MQ=255)
                                                                         aTCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAggg                                                                             <  2:809677/150‑1 (MQ=255)
                                                                          tCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGa                                                                            >  2:712348/1‑150 (MQ=255)
                                                                                tGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAgt                                                                      <  1:409599/150‑1 (MQ=255)
                                                                                    gTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGTGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTgg                                                                  <  2:635658/150‑1 (MQ=255)
                                                                                     tATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTggg                                                                 <  1:18902/150‑1 (MQ=255)
                                                                                     tATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTggg                                                                 <  1:18773/150‑1 (MQ=255)
                                                                                     tATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTggg                                                                 <  1:256170/150‑1 (MQ=255)
                                                                                     tATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTggg                                                                 <  2:1272574/150‑1 (MQ=255)
                                                                                       tctcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGa                                                               >  2:637081/1‑150 (MQ=255)
                                                                                       tctcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGa                                                               >  2:1213108/1‑150 (MQ=255)
                                                                                       tctcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGa                                                               >  2:270684/1‑150 (MQ=255)
                                                                                         tcATGCAAACAAACCCTTGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGaaa                                                             <  1:618848/150‑1 (MQ=255)
                                                                                         tcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAATGTGTGGGGaaa                                                             >  2:485718/1‑150 (MQ=255)
                                                                                         tcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGaaa                                                             >  2:687871/1‑150 (MQ=255)
                                                                                         tcATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGaaa                                                             >  2:1393719/1‑150 (MQ=255)
                                                                                            tGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAgtgt                                                                    <  1:551610/140‑1 (MQ=255)
                                                                                            tGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAgtgt                                                                    >  2:551610/1‑140 (MQ=255)
                                                                                            tGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAagag                                                          >  2:610563/1‑150 (MQ=255)
                                                                                               aaacaaacCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAgtgt                                                       <  1:666251/150‑1 (MQ=255)
                                                                                                           gACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGcaca                                           <  1:236260/150‑1 (MQ=255)
                                                                                                                aCAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAAttt                                      >  2:531646/1‑150 (MQ=255)
                                                                                                                 cAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTa                                     <  1:53295/150‑1 (MQ=255)
                                                                                                                 cAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTa                                     <  1:768532/150‑1 (MQ=255)
                                                                                                                  aGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTAc                                    >  2:1650182/1‑150 (MQ=255)
                                                                                                                  aGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTAc                                    <  1:1648826/150‑1 (MQ=255)
                                                                                                                    gTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaa                                  >  2:181033/1‑150 (MQ=255)
                                                                                                                     tGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaaa                                 >  2:713487/1‑150 (MQ=255)
                                                                                                                     tGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaaa                                 >  2:713517/1‑150 (MQ=255)
                                                                                                                            acCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCaa                          <  1:413383/150‑1 (MQ=255)
                                                                                                                              ccTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAGGCACAAATTTACAAAAATGCAAGt                        >  2:142412/1‑150 (MQ=255)
                                                                                                                              ccTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGt                        >  2:515662/1‑150 (MQ=255)
                                                                                                                              ccTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGt                        >  2:142421/1‑150 (MQ=255)
                                                                                                                                   ttAGTGGCTTTTCAGGCTCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaa                   <  1:387117/150‑1 (MQ=255)
                                                                                                                                   ttAGTGGCTTTTCAGGCTCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaa                   <  1:387120/150‑1 (MQ=255)
                                                                                                                                   ttAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaa                   <  1:968771/150‑1 (MQ=255)
                                                                                                                                    tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaa                  <  1:630303/150‑1 (MQ=255)
                                                                                                                                         gCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAgaga             >  2:831052/1‑150 (MQ=255)
                                                                                                                                           ttttCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACCCGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACt           >  2:443641/1‑150 (MQ=255)
                                                                                                                                           ttttCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACt           >  2:443639/1‑150 (MQ=255)
                                                                                                                                                    ccGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa  <  1:1044413/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCGGTAAAAAACCGCGCCATTCATAAAAAGGCCAATATAGATTGCAAGCAAAAAGCTCAGCTTCTGCTGTGTAATCGATTTGATGTATCTCATGCAAACAAACCCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTA  >  NC_000913/3710383‑3710680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: