Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,518 A→G 5.2% intergenic (+22/+84) yiiM → / ← cpxA 6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,5180AG 5.2% 386.5 / 8.6 115intergenic (+22/+84)yiiM/cpxA6‑N‑hydroxylaminopurine resistance protein/sensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (57/52);  new base G (3/3);  total (60/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 117 total aligned reads displayed.

GACGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGC  >  NC_000913/4103374‑4103667
                                                                                                                                                |                                                                                                                                                     
gaCGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACg                                                                                                                                                  >  2:821341/1‑150 (MQ=255)
gaCGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACg                                                                                                                                                  >  2:299928/1‑150 (MQ=255)
gaCGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACg                                                                                                                                                  >  2:1516355/1‑150 (MQ=255)
gaCGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACg                                                                                                                                                  >  2:1289434/1‑150 (MQ=255)
    aTCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGACTGATAAGACGCTAt                                                                                                                                              >  1:1595127/1‑150 (MQ=255)
      cAATCTCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCGGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                            <  2:174516/150‑1 (MQ=255)
      cAATATCCCCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                            <  1:645363/150‑1 (MQ=255)
      cAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                            <  2:408353/150‑1 (MQ=255)
      cAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCa                                                                                                                                            <  2:1392601/150‑1 (MQ=255)
          atCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGt                                                                                                                                        <  1:1516219/150‑1 (MQ=255)
           tCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCACAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGACAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTCTCAGCGTc                                                                                                                                       >  1:463046/1‑150 (MQ=255)
           tCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTc                                                                                                                                       >  1:332234/1‑150 (MQ=255)
           tCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTc                                                                                                                                       >  1:332250/1‑150 (MQ=255)
              ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATGCGACGCTGATAGCGTctta                                                                                                                                    <  1:1469780/150‑4 (MQ=255)
              ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATGCGACGCTGATAGCGTctta                                                                                                                                    <  1:1403160/150‑4 (MQ=255)
              ccGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATGCGACGCTGATAGCGTctta                                                                                                                                    <  1:1403099/150‑4 (MQ=255)
               cGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCAt                                                                                                                                   >  1:191839/1‑150 (MQ=255)
                 tttACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCa                                                                                                                                 >  2:126247/1‑150 (MQ=255)
                  ttACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAg                                                                                                                                <  1:352125/150‑1 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  2:1442944/1‑150 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  1:862394/1‑150 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  1:1340235/1‑150 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  1:1340113/1‑150 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  1:624616/1‑150 (MQ=255)
                      tctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAt                                                                                                                            >  1:1340097/1‑150 (MQ=255)
                       ctcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATc                                                                                                                           <  2:1034130/150‑1 (MQ=255)
                        tcCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATcc                                                                                                                          <  1:329906/150‑1 (MQ=255)
                          cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTg                                                                                                                        <  1:696227/150‑1 (MQ=255)
                          cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTg                                                                                                                        <  1:189028/150‑1 (MQ=255)
                          cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTg                                                                                                                        >  2:1373349/1‑150 (MQ=255)
                          cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTg                                                                                                                        >  2:1679213/1‑150 (MQ=255)
                          cGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCATCGCATCAGGCATCCTg                                                                                                                        >  2:338372/1‑150 (MQ=255)
                                   gTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGc                                                                                                               <  2:996132/150‑1 (MQ=255)
                                         gAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATg                                                                                                         >  2:1040848/1‑150 (MQ=255)
                                         gAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAGCCTGCTCAAATGCCGGATg                                                                                                         >  2:1649959/1‑150 (MQ=255)
                                          aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgc                                                                                                        >  1:866328/1‑150 (MQ=255)
                                          aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgc                                                                                                        >  1:866318/1‑150 (MQ=255)
                                          aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgc                                                                                                        >  2:1503784/1‑150 (MQ=255)
                                          aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgc                                                                                                        >  2:1503706/1‑150 (MQ=255)
                                          aaaaGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATgc                                                                                                        >  1:1346398/1‑150 (MQ=255)
                                                  gACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaaa                                                                                                <  1:67266/150‑1 (MQ=255)
                                                  gACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTaaa                                                                                                <  1:957599/150‑1 (MQ=255)
                                                     gcgGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGc                                                                                             >  2:777473/1‑150 (MQ=255)
                                                     gcgGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGc                                                                                             >  1:105739/1‑150 (MQ=255)
                                                       ggACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCt                                                                                           <  2:1529548/150‑1 (MQ=255)
                                                        gACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCtt                                                                                          >  2:800178/1‑150 (MQ=255)
                                                        gACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCtt                                                                                          >  2:800200/1‑150 (MQ=255)
                                                          cGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTAt                                                                                        <  1:1524750/150‑1 (MQ=255)
                                                               cAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgc                                                                                   <  1:899204/150‑1 (MQ=255)
                                                               cAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgc                                                                                   <  1:1398152/150‑1 (MQ=255)
                                                                aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcc                                                                                  >  1:54600/1‑150 (MQ=255)
                                                                aaaaGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATcctgcc                                                                                  >  2:1224740/1‑150 (MQ=255)
                                                                       ccGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCATATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCACAt                                                                           >  2:914109/1‑150 (MQ=255)
                                                                        cGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCGTATCCGGCCGGCacgtg                                                                          >  1:1477027/1‑146 (MQ=255)
                                                                         gACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCGTATCCTGCCTGCAAATGc                                                                         >  2:958576/1‑150 (MQ=255)
                                                                          aCTGAGCGGCAAGATCGAAGATTGTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCg                                                                        <  2:1161314/150‑1 (MQ=255)
                                                                            tGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGaa                                                                      <  2:2636/150‑1 (MQ=255)
                                                                                 ggCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTGTa                                                                 >  2:1408513/1‑150 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:1289533/150‑1 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:1072629/150‑1 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:1289434/150‑1 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:299928/150‑1 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:1516355/150‑1 (MQ=255)
                                                                                  gCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTaa                                                                <  1:821341/150‑1 (MQ=255)
                                                                                    aaGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACt                                                              >  1:757495/1‑150 (MQ=255)
                                                                                    aaGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACt                                                              >  1:757481/1‑150 (MQ=255)
                                                                                        tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                          <  2:786515/150‑1 (MQ=255)
                                                                                        tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                          <  2:405244/150‑1 (MQ=255)
                                                                                        tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                          >  1:187969/1‑150 (MQ=255)
                                                                                        tCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                          <  2:1497505/150‑1 (MQ=255)
                                                                                        acGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGc                                                          <  2:60269/149‑1 (MQ=255)
                                                                                                  ttCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCg                                                <  1:493916/150‑1 (MQ=255)
                                                                                                      cgccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCaa                                            >  1:1568223/1‑150 (MQ=255)
                                                                                                       gccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAAc                                           <  2:967932/150‑1 (MQ=255)
                                                                                                                  ggAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAg                                <  1:1593275/150‑1 (MQ=255)
                                                                                                                      aaTAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGt                            <  1:126247/150‑1 (MQ=255)
                                                                                                                           cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAcc                       <  1:317264/150‑1 (MQ=255)
                                                                                                                           cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCAACCGGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAAcc                       <  2:1274205/150‑1 (MQ=255)
                                                                                                                            ccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCg                      <  1:1224740/150‑1 (MQ=255)
                                                                                                                              ccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGcc                    >  1:636850/1‑150 (MQ=255)
                                                                                                                                   gtgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCg               <  2:132915/150‑1 (MQ=255)
                                                                                                                                   gtgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCg               <  2:132956/150‑1 (MQ=255)
                                                                                                                                    tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg              >  1:1242635/1‑150 (MQ=255)
                                                                                                                                    tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg              >  2:702059/1‑150 (MQ=255)
                                                                                                                                    tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg              <  1:1518252/150‑1 (MQ=255)
                                                                                                                                    tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCgg              >  1:720552/1‑150 (MQ=255)
                                                                                                                                     gtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAATTGCCGGATGCGGCGTATACGCCTTATCCTGCCTGCATATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCGCAGCgtg             <  2:132150/150‑3 (MQ=255)
                                                                                                                                       aGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCt           <  1:1711009/150‑1 (MQ=255)
                                                                                                                                         gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGt         >  1:1047219/1‑150 (MQ=255)
                                                                                                                                         gCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGt         >  1:1390676/1‑150 (MQ=255)
                                                                                                                                         gCCTGATAAGACGCTATCAGCGACGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGt         >  2:1272241/1‑150 (MQ=255)
                                                                                                                                          ccTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtc        >  2:1717777/1‑150 (MQ=38)
                                                                                                                                           cTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct       >  1:980433/1‑150 (MQ=38)
                                                                                                                                           cTGATGCGACGCTGATAGCGTCTTATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtct       >  1:980425/1‑150 (MQ=38)
                                                                                                                                            tGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAACCCGCCCAGCGGGCTGtctt      >  2:734804/1‑150 (MQ=255)
                                                                                                                                             gATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttc     <  1:188177/150‑1 (MQ=255)
                                                                                                                                              ctAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttct    <  1:768160/149‑1 (MQ=255)
                                                                                                                                              aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGtcttct    <  1:804789/150‑1 (MQ=255)
                                                                                                                                                aaGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGc  >  2:444319/1‑150 (MQ=255)
                                                                                                                                                aaGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGc  <  2:1593063/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GACGATCAATATCACCGTTTACTCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCCGACTGAGCGGCAAGATCGAAGATTTTTCCCGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTCAAATGCCGGATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACAGCGGCAACCAAATCACCAGCCGTAAACCGCCCAGCGGGCTGTCTTCTGC  >  NC_000913/4103374‑4103667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: