Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 551,489 A→C 5.3% intergenic (+157/+38) ybcF → / ← purK putative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913551,4890AC 5.3% 539.7 / 15.6 151intergenic (+157/+38)ybcF/purKputative carbonate kinase/N5‑carboxyaminoimidazole ribonucleotide synthase
Reads supporting (aligned to +/- strand):  ref base A (77/66);  new base C (2/6);  total (79/72)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 157 total aligned reads displayed.

CATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAA  >  NC_000913/551340‑551638
                                                                                                                                                     |                                                                                                                                                     
  ttAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAgg                                                                                                                                                     >  1:1011383/1‑150 (MQ=255)
  ttAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAgg                                                                                                                                                     >  1:1011366/1‑150 (MQ=255)
  ttAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAgg                                                                                                                                                     >  1:1350723/1‑150 (MQ=255)
  ttAGACATATGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTCCAATGGGTACCGGATCGGTAGGCCGGATAAgg                                                                                                                                                     >  2:1598444/1‑150 (MQ=255)
    aGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCg                                                                                                                                                   >  1:1552803/1‑150 (MQ=255)
     gACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGt                                                                                                                                                  <  2:489718/150‑1 (MQ=255)
     gACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGt                                                                                                                                                  <  2:56597/150‑1 (MQ=255)
      tcATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGtt                                                                                                                                                 <  1:1329312/149‑1 (MQ=255)
          ttGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTAcg                                                                                                                                             >  1:390188/1‑150 (MQ=255)
           tgtgCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                            >  2:142979/1‑150 (MQ=255)
            gtgCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTAcgcc                                                                                                                                           <  1:1179674/150‑1 (MQ=255)
             tgCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  1:1140730/150‑1 (MQ=255)
             tgCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          >  2:1236701/1‑150 (MQ=255)
                cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                       <  1:1450831/150‑5 (MQ=37)
                cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                       <  1:205093/150‑5 (MQ=37)
                cTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATGCGGCGTAAAcgccttat                                                                                                                                       <  2:850219/150‑5 (MQ=37)
                 tGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                      >  2:294460/1‑150 (MQ=255)
                   aTGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                    <  2:1706144/150‑1 (MQ=255)
                   aTGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                    <  1:535818/150‑1 (MQ=255)
                    agCGACGCTTGACGCGTCTTATCAGGCCAACAACCGGTGCCGCATCCGGCAAGTGGTGCACCATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTCCAAGGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                   <  1:638840/149‑1 (MQ=38)
                     gCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                  <  2:252137/150‑1 (MQ=37)
                       gACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCac                                                                                                                                >  2:1598937/1‑149 (MQ=255)
                       gACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                                >  2:117121/1‑150 (MQ=255)
                       gACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaa                                                                                                                                <  2:383395/150‑1 (MQ=255)
                         cGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGa                                                                                                                              <  1:1458041/150‑1 (MQ=255)
                          gCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGaa                                                                                                                             >  2:1115535/1‑150 (MQ=255)
                               aCGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACGAATagag                                                                                                                        >  1:1495833/1‑150 (MQ=255)
                               aCGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACGAATagag                                                                                                                        >  2:258864/1‑150 (MQ=255)
                               aCGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATagag                                                                                                                        <  2:1114053/150‑1 (MQ=255)
                                 gcgTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCa                                                                                                                      <  1:99545/150‑1 (MQ=255)
                                   gTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCAcc                                                                                                                    <  2:444095/150‑1 (MQ=255)
                                           aGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACGAATAGAGCACCCGTTAAcc                                                                                                            >  2:513684/1‑150 (MQ=255)
                                           aGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAAcc                                                                                                            >  2:874024/1‑150 (MQ=255)
                                           aGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAAcc                                                                                                            >  2:6910/1‑150 (MQ=255)
                                              ccTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGaa                                                                                                         <  2:1350723/150‑1 (MQ=255)
                                              ccTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGATTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGaa                                                                                                         <  2:1011383/150‑1 (MQ=255)
                                               cTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAAc                                                                                                        >  2:959515/1‑150 (MQ=255)
                                               cTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAAc                                                                                                        >  2:1632950/1‑150 (MQ=255)
                                               cTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAAc                                                                                                        >  2:898411/1‑150 (MQ=255)
                                                  cAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTa                                                                                                     <  1:172519/150‑1 (MQ=255)
                                                  cAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTa                                                                                                     <  2:1124488/150‑1 (MQ=255)
                                                     ccGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTActc                                                                                                  <  1:909222/150‑1 (MQ=255)
                                                       ggTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGTCAAGAATAGAGCACCAGTTAACCGAACTTACTCTg                                                                                                >  2:592009/1‑150 (MQ=255)
                                                          gCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTgcgc                                                                                             <  1:1127290/150‑1 (MQ=255)
                                                          gCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTgcgc                                                                                             <  1:405432/150‑1 (MQ=255)
                                                          gCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTgcgc                                                                                             <  1:405601/150‑1 (MQ=255)
                                                                 ccGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATc                                                                                      <  1:1318427/150‑1 (MQ=255)
                                                                   ggCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCAc                                                                                    <  2:845723/150‑1 (MQ=255)
                                                                     cAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCAcgc                                                                                  >  2:204082/1‑150 (MQ=255)
                                                                           gTGCACAATGCCTGTTGCGATGCTTGACGCATCTTATCAGGCCTACATTGGGTACCGGTTGGGTTGGCCGGATAAGGCGTTTACGCCGCATCCGGAAAGAATAGAGCACCTGTTAACCTAACTTACTCTGCGCCCAAATCACGCCGCTgg                                                                            <  1:520780/150‑1 (MQ=37)
                                                                           gTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTgg                                                                            >  2:357666/1‑150 (MQ=255)
                                                                           gTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTgg                                                                            >  2:163444/1‑150 (MQ=255)
                                                                           gTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAAACGAACTTAATCTTCTCCAAAATCACGCCGCTgg                                                                            >  2:1452857/1‑150 (MQ=38)
                                                                            tGCACAAGGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGc                                                                           <  1:1641348/150‑1 (MQ=255)
                                                                                   ggCCTGATAAGATGCGTCAAGCATCGCATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTcc                                                                    >  1:1302484/2‑150 (MQ=38)
                                                                                      cTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCcggc                                                                 >  1:1605406/1‑150 (MQ=255)
                                                                                         atgcgatgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCggcggc                                                              >  2:1195484/1‑150 (MQ=255)
                                                                                            cgatgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAATTCACGCCGCTGGCATATTCCGGCGgcaac                                                           >  2:611259/1‑148 (MQ=255)
                                                                                            cgatgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGgcagc                                                           >  1:684052/1‑150 (MQ=255)
                                                                                             gatgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGgcagca                                                          >  2:1330056/1‑150 (MQ=255)
                                                                                             gatgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGgcagca                                                          >  2:1169802/1‑150 (MQ=255)
                                                                                              atgcTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGcagcag                                                         >  2:356421/1‑150 (MQ=255)
                                                                                                 cTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCgg                                                      <  1:1468820/150‑1 (MQ=255)
                                                                                                  ttGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCggg                                                     >  2:416876/1‑150 (MQ=255)
                                                                                                  ttGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCggg                                                     >  2:944408/1‑150 (MQ=255)
                                                                                                       gCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTaa                                                <  1:1576417/150‑1 (MQ=255)
                                                                                                        cATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGTGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAg                                               <  2:1280006/150‑1 (MQ=255)
                                                                                                        cATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAg                                               <  1:1151062/150‑1 (MQ=255)
                                                                                                             tATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTc                                          >  2:197197/1‑150 (MQ=255)
                                                                                                             tATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTc                                          <  2:1036761/150‑1 (MQ=255)
                                                                                                               tCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCa                                        <  1:574667/150‑1 (MQ=255)
                                                                                                               tCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCa                                        <  1:74728/150‑1 (MQ=255)
                                                                                                                    ccTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTc                                   <  2:1222264/150‑1 (MQ=255)
                                                                                                                     cTACACTGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGACTAGAGCACCAGTTAACCGAACTTACTGTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCg                                  <  2:308336/150‑1 (MQ=255)
                                                                                                                     cTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCg                                  >  1:630485/1‑150 (MQ=255)
                                                                                                                     cTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCg                                  <  1:659337/150‑1 (MQ=255)
                                                                                                                      tACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCAACCAGCGTCGc                                 >  1:1087614/1‑150 (MQ=255)
                                                                                                                            gggTCCCGGATCGGTAGGCCGGATAAGGCGTTTACGACGCATCCGGCAAGAATAGAGCACCAGGTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGAGGGATTAAGGCTTCCAGCGTCGCAGTCAg                           >  2:1511534/1‑150 (MQ=255)
                                                                                                                             ggTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGa                          >  1:1356025/1‑150 (MQ=255)
                                                                                                                             ggTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGa                          >  2:1277753/1‑150 (MQ=255)
                                                                                                                              gTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATCTTCCGGCGGCAGCAGCGGGAGTAAGGCTTCCAGCGTCGCAGTCAGAc                         >  1:877977/1‑150 (MQ=255)
                                                                                                                              gTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGAc                         >  1:1109205/1‑150 (MQ=255)
                                                                                                                                  cGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGa                     <  1:959515/150‑1 (MQ=255)
                                                                                                                                   ggATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGAt                    >  1:430057/1‑150 (MQ=255)
                                                                                                                                    gATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGAtg                   >  1:1156108/1‑150 (MQ=255)
                                                                                                                                    gATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGAtg                   >  2:1720351/1‑150 (MQ=255)
                                                                                                                                    gATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGAAGCGGGATTAAGGCTTCAAGCGTCGCTGTCAGACGCGAtg                   >  2:1161542/1‑150 (MQ=255)
                                                                                                                                      tCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGAtgtg                 <  1:1195484/150‑1 (MQ=255)
                                                                                                                                      tCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGAtgtg                 <  1:1195490/150‑1 (MQ=255)
                                                                                                                                         ggaGGCCGGAGAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTTCTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCg              <  1:1598937/148‑1 (MQ=255)
                                                                                                                                         gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCg              >  2:202681/1‑150 (MQ=255)
                                                                                                                                             gCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGt          >  2:703072/1‑150 (MQ=255)
                                                                                                                                              ccGGATGCGGCGTAAACGCCTTATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTc         >  2:425019/1‑150 (MQ=255)
                                                                                                                                              ccGGATGCGGCGTAAACGCCTTATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTACGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTc         >  1:779444/1‑150 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTc         <  1:715071/150‑1 (MQ=255)
                                                                                                                                              ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTc         >  2:943454/1‑150 (MQ=255)
                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCg        <  2:1156108/150‑1 (MQ=255)
                                                                                                                                                     aGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCaaa  >  1:934945/1‑150 (MQ=255)
                                                                                                                                                     aGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCaaa  >  1:934965/1‑150 (MQ=255)
                                                                                                                                                     aGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCaaa  <  1:729834/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTACCGGATCGGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAATAGAGCACCAGTTAACCGAACTTACTCTGCGCCCAAATCACGCCGCTGGCATATTCCGGCGGCAGCAGCGGGATTAAGGCTTCCAGCGTCGCAGTCAGACGCGATGTGTCGCTGTCGGTCAAA  >  NC_000913/551340‑551638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: