Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,580 G→A 9.3% intergenic (+320/+100) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,5800GA 9.3% 349.8 / 16.0 115intergenic (+320/+100)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (63/38);  new base A (5/5);  total (68/47)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACC  >  NC_000913/4614433‑4614725
                                                                                                                                                   |                                                                                                                                                 
tCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTAGGCGCAAAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACATGTATGTGCCGAACCGTAGGCCGGATACGGCGTgcgcc                                                                                                                                                 >  2:19278/1‑149 (MQ=11)
  aTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc                                                                                                                                               >  1:1462323/1‑150 (MQ=255)
  aTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc                                                                                                                                               >  1:1462329/1‑150 (MQ=255)
  aTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTAAAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc                                                                                                                                               >  2:1655122/1‑150 (MQ=255)
   tGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCAAAATGCCTGAAGCGACGCTTCTCGCGTCTTATCATGCCTACAGCTTTGTGCCGAACCGTAGGCAGGATAAGGCGTTCGcgccg                                                                                                                                              >  2:132643/1‑150 (MQ=14)
    gCCTACAGGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgccgc                                                                                                                                             <  1:616447/150‑1 (MQ=255)
       tACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATc                                                                                                                                          >  1:1682814/1‑150 (MQ=255)
         cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCg                                                                                                                                        <  2:262658/150‑1 (MQ=255)
          ctgTCTGTTCCGATCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCgg                                                                                                                                       <  2:313053/148‑1 (MQ=255)
          aaGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCgg                                                                                                                                       <  1:11724/150‑1 (MQ=255)
           aGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGc                                                                                                                                      >  2:1428122/1‑150 (MQ=255)
               tgtgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGtt                                                                                                                                  <  1:432975/150‑1 (MQ=255)
                 tgCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTgg                                                                                                                                >  2:314613/1‑150 (MQ=255)
                  aacgtgccGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCAGAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGcgcc                                                                                                                                               >  2:445862/7‑134 (MQ=255)
                     gAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGca                                                                                                                            >  1:157544/1‑150 (MQ=255)
                       cccGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCACAATGCCTGATGCGACGCTTTTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGcaca                                                                                                                          <  2:1662774/149‑1 (MQ=21)
                       cccGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCAGAATGCCTGATGCGACGCTTCTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGcaca                                                                                                                          <  2:1662779/149‑1 (MQ=255)
                             ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                                                    <  1:1046165/150‑1 (MQ=255)
                             cgCCGGAGAAGGCGATCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGATTGTGCAGATCAGTAGTCCGGATATGTCTTTCGCTCCGCATCCGGCAGTTGGCGCACAATGCCt                                                                                                                    <  1:1046166/149‑1 (MQ=14)
                              gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTg                                                                                                                   >  1:1392976/1‑150 (MQ=255)
                                  gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACCGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                <  2:1369789/149‑1 (MQ=255)
                                  gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                >  1:1369789/1‑149 (MQ=255)
                                  gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                >  1:1369784/1‑149 (MQ=255)
                                  gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                <  2:1369784/149‑1 (MQ=255)
                                  gATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATg                                                                                                                <  2:1369844/149‑1 (MQ=255)
                                   aTAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCg                                                                                                              >  1:1341561/1‑150 (MQ=255)
                                       ggCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGc                                                                                                          <  2:1483409/150‑1 (MQ=255)
                                           ttCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGa                                                                                                      <  2:357281/150‑1 (MQ=255)
                                                gccgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGt                                                                                                 >  2:366792/1‑150 (MQ=255)
                                                gccgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGt                                                                                                 >  1:239869/1‑150 (MQ=255)
                                                    cATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTa                                                                                             >  2:258981/1‑150 (MQ=255)
                                                    cATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTa                                                                                             >  1:424723/1‑150 (MQ=255)
                                                         ggCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAgg                                                                                        >  1:17031/1‑150 (MQ=255)
                                                                        aTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGc                                                                         <  2:1682814/150‑1 (MQ=255)
                                                                           ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGa                                                                      >  1:728495/1‑150 (MQ=255)
                                                                            cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGaa                                                                     >  1:219589/1‑150 (MQ=255)
                                                                            cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGaa                                                                     >  1:1568167/1‑150 (MQ=255)
                                                                             tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAAc                                                                    >  2:1517090/1‑150 (MQ=255)
                                                                              gATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAATGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAAcc                                                                   >  1:1361333/1‑150 (MQ=255)
                                                                                tGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTGGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGt                                                                 >  1:736171/1‑150 (MQ=255)
                                                                                tGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGt                                                                 >  2:1518928/1‑150 (MQ=255)
                                                                                 gCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTa                                                                >  2:261498/1‑150 (MQ=255)
                                                                                   gACTCTTGTCTCGTCTTATCATGGCTACAGTTTTTTTCCGATCCGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAAGTATGTGCCGAACCGTAgg                                                              <  2:612140/150‑1 (MQ=11)
                                                                                             gcgTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCgg                                                    <  2:1435668/150‑1 (MQ=255)
                                                                                               gTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCa                                                  <  2:522115/150‑1 (MQ=255)
                                                                                                tcgtctCATGCCTACAGGTTTGGGCCGCCCCGTAGGCCGGATACGGCGTTCGCGCCGCCTCCGGCAGTTGGCGCACCATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCAt                                                 <  2:1700215/145‑1 (MQ=14)
                                                                                                tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCtgt                                                                           <  2:1192840/124‑1 (MQ=38)
                                                                                                tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCAt                                                 >  1:1205135/1‑150 (MQ=255)
                                                                                                tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCtgt                                                                           >  1:1192840/1‑124 (MQ=17)
                                                                                                tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCtgt                                                                           <  2:160490/124‑1 (MQ=17)
                                                                                                tCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCtgt                                                                           <  2:160500/124‑1 (MQ=17)
                                                                                                   tATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTc                                              <  2:657855/150‑1 (MQ=12)
                                                                                                    aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTca                                             <  1:467099/150‑1 (MQ=255)
                                                                                                     tCATGCCTACAGGTTTGTGCCTAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                            >  2:775097/1‑150 (MQ=255)
                                                                                                     tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                            >  2:450099/1‑150 (MQ=255)
                                                                                                     tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                            >  2:1125902/1‑150 (MQ=255)
                                                                                                     tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                            >  2:271637/1‑150 (MQ=255)
                                                                                                        tGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACaaa                                         >  1:762869/1‑150 (MQ=255)
                                                                                                          cgttcAGGTTTGTGCCGATCCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATa                                       <  1:774062/146‑1 (MQ=255)
                                                                                                          ccTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATa                                       <  2:799042/150‑1 (MQ=255)
                                                                                                           cTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATag                                      <  1:414824/150‑1 (MQ=255)
                                                                                                            tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGcc                                                                                                                     <  2:775324/70‑1 (MQ=35)
                                                                                                            tACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATaga                                     >  2:1201544/1‑150 (MQ=255)
                                                                                                               aGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAgcg                                  >  2:1004717/1‑150 (MQ=255)
                                                                                                               aGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAgcg                                  >  1:44546/1‑150 (MQ=255)
                                                                                                                 gTTTGTGCCGAACCGTAGGCCGGATAAGGTGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGcc                                >  1:1627497/1‑150 (MQ=255)
                                                                                                                 gTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGcc                                <  1:987271/150‑1 (MQ=255)
                                                                                                                  tttGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCg                               >  2:359758/1‑150 (MQ=255)
                                                                                                                       gCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCg                                                                  <  1:401693/110‑1 (MQ=40)
                                                                                                                        ccGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAata                         >  1:135809/1‑150 (MQ=255)
                                                                                                                        ccGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAata                         >  1:232553/1‑150 (MQ=255)
                                                                                                                         cGATCCGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAatat                        <  2:1502645/150‑1 (MQ=255)
                                                                                                                         cGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAatat                        <  1:750488/150‑1 (MQ=255)
                                                                                                                             ccGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCAGGCATGACACAAAAATAGCGCCGGaaaaaaaaac                    >  2:565714/1‑143 (MQ=14)
                                                                                                                              cGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                 <  2:862674/88‑1 (MQ=255)
                                                                                                                                    ccGGATGCGGCGTGAACGCCTTATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCa             >  2:889628/1‑150 (MQ=18)
                                                                                                                                      ggATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAcc           >  2:1650435/1‑150 (MQ=255)
                                                                                                                                       gATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCAccc          >  1:898844/1‑150 (MQ=255)
                                                                                                                                       gATAAGGAGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGAGTCTTATCAGGCCTACAAGTCTGTGCCGAAACGTAGGCCTTATCCGGCATGTCACAAATAGAGCGCCGGAAATATAAAACGCCTCAccc          >  1:1178846/1‑150 (MQ=21)
                                                                                                                                         tAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACATGTCTGTGCCGAACCGTAGGCCTTATCCGGCATGTCACAAATAGAGCGCCGTAAATATCAACCGGCTCACCCcg        <  1:565714/150‑1 (MQ=255)
                                                                                                                                         tAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCcg        <  1:1358495/150‑1 (MQ=255)
                                                                                                                                          acggCGTTCGCGCCGCATCCGGAAGTTGGCGCACAATGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACACGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGTAAATATCAACCGGCTCACCCcgc       <  1:577860/148‑1 (MQ=255)
                                                                                                                                          aaGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCcgc       <  1:577835/150‑1 (MQ=255)
                                                                                                                                            ggCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACACATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgc     >  2:1486580/1‑150 (MQ=255)
                                                                                                                                            ggCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgc     >  1:933552/1‑150 (MQ=255)
                                                                                                                                            ggCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgc     >  2:278416/1‑150 (MQ=255)
                                                                                                                                            ggCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgc     >  1:1103251/1‑150 (MQ=255)
                                                                                                                                            ggCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgc     >  1:1075914/1‑150 (MQ=255)
                                                                                                                                             gCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCgcgcc    >  1:1095450/1‑149 (MQ=255)
                                                                                                                                             gCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCa    >  2:333457/1‑150 (MQ=255)
                                                                                                                                             gCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCa    >  1:1383242/1‑150 (MQ=255)
                                                                                                                                             gCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCa    >  1:521753/1‑150 (MQ=255)
                                                                                                                                             gCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCa    >  1:521760/1‑150 (MQ=255)
                                                                                                                                               gTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCAGAAATATCAACCGGCTCACCCCGCGCAcc  >  1:321662/1‑150 (MQ=255)
                                                                                                                                                ttCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCgg                 <  1:119967/134‑1 (MQ=255)
                                                                                                                                                ttCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCgg                 >  2:119964/1‑134 (MQ=255)
                                                                                                                                                ttCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCgg                 <  1:119964/134‑1 (MQ=255)
                                                                                                                                                ttCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCgg                 >  2:119967/1‑134 (MQ=255)
                                                                                                                                                ttCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACAAGTCTGTGcc                                                                        >  1:1023441/1‑79 (MQ=255)
                                                                                                                                                ttCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACAAGTCTGTGcc                                                                        <  2:1023441/79‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACC  >  NC_000913/4614433‑4614725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: