Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 749,648 A→T 5.7% A20A (GCT→GCA ybgP ← putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913749,6480AT 5.7% 341.1 / 9.1 97A20A (GCT→GCAybgPputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base A (41/50);  new base T (4/2);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

CCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTGCTCA  >  NC_000913/749500‑749797
                                                                                                                                                    |                                                                                                                                                     
ccAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCGCTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCgcag                                                                                                                                                      <  1:626086/150‑1 (MQ=255)
 cAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCgcagc                                                                                                                                                     <  1:877647/150‑1 (MQ=255)
 cAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCgcagc                                                                                                                                                     <  1:691125/150‑1 (MQ=255)
    cagagCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCAtt                                                                                                                                                  <  1:1578681/149‑1 (MQ=255)
    aagaGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCAtt                                                                                                                                                  <  1:511133/150‑1 (MQ=255)
    aagaGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCAtt                                                                                                                                                  <  1:65413/150‑1 (MQ=255)
              cTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCAATGCTGCGGTGCAAcccgat                                                                                                                                        <  2:1426655/150‑4 (MQ=255)
               ttttCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGc                                                                                                                                       <  2:214890/150‑1 (MQ=255)
               ttttCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGc                                                                                                                                       >  2:304484/1‑150 (MQ=255)
               ttttCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCACAGCATTGCAACCCAAGc                                                                                                                                       >  2:1053870/1‑150 (MQ=255)
                   ctcCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGAt                                                                                                                                   <  1:525662/150‑1 (MQ=255)
                    tcCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATg                                                                                                                                  >  1:1646389/1‑150 (MQ=255)
                     ccccTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATgg                                                                                                                                 >  2:135871/1‑150 (MQ=255)
                     ccccTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATgg                                                                                                                                 >  2:708272/1‑150 (MQ=255)
                      cccTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGt                                                                                                                                >  1:428673/1‑150 (MQ=255)
                        cTTTTTCAATTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTa                                                                                                                              <  1:1309515/150‑1 (MQ=255)
                                  ttCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTaa                                                                                                                    >  1:1337959/1‑150 (MQ=255)
                                  ttCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTaa                                                                                                                    <  2:1431330/150‑1 (MQ=255)
                                  ttCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTaa                                                                                                                    <  2:187967/150‑1 (MQ=255)
                                         cAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAAt                                                                                                             >  1:262698/1‑150 (MQ=255)
                                          aGGAATAAGCAAGATAAGGCAGTTTGTCGCTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATc                                                                                                            >  1:154283/1‑150 (MQ=255)
                                                 aGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTtaat                                                                                                     >  2:701972/1‑150 (MQ=255)
                                                  gCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTtaata                                                                                                    >  1:19254/1‑150 (MQ=255)
                                                    aaGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATaaa                                                                                                  <  1:115177/150‑1 (MQ=255)
                                                    aaGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATaaa                                                                                                  <  1:115236/150‑1 (MQ=255)
                                                    aaGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATaaa                                                                                                  <  1:102243/150‑1 (MQ=255)
                                                     aGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAAt                                                                                                 <  1:935315/150‑1 (MQ=255)
                                                     aGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAAt                                                                                                 <  2:819637/150‑1 (MQ=255)
                                                       aTAAGGCAGTTTGACACTTAGGTTTTCGATACGAAGGCTGGTGTCATTGTAATTGTCGTAAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTTATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGt                                                                                               >  2:795894/1‑150 (MQ=255)
                                                           ggCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCAtt                                                                                           <  1:712163/150‑1 (MQ=255)
                                                           ggCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCAtt                                                                                           >  2:1517234/1‑150 (MQ=255)
                                                            gCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATtc                                                                                          >  2:290122/1‑150 (MQ=255)
                                                            gCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAAAGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCATTAATAAATGTCATtc                                                                                          >  1:453004/1‑150 (MQ=255)
                                                                 ttGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCttt                                                                                     <  1:538016/150‑1 (MQ=255)
                                                                  tGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCtttt                                                                                    >  2:20765/1‑150 (MQ=255)
                                                                   gTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACACGGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGCCATTCTCTTGTc                                                                                   <  2:390462/150‑1 (MQ=255)
                                                                   gTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTc                                                                                   <  2:859621/150‑1 (MQ=255)
                                                                   gTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTc                                                                                   <  1:877382/150‑1 (MQ=255)
                                                                   gTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTc                                                                                   <  2:520223/150‑1 (MQ=255)
                                                                   gTCACTTTGGTTTTCGATACGCAGGCTGGTGGCATTGTCATTGGCGTTACTCACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCATTTAATAAATGTCATTCTCTTTTc                                                                                   <  2:259136/150‑1 (MQ=255)
                                                                   gTCACTTTGGTTGTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACTATGCGGTTGCGATCGGGTTGCACCGCAGCATTGGAACCCAAGATGATGGTTAACAAGATTAAGTGTAATCCTTTAATATATGTCAGTCTCTTTTc                                                                                   <  2:1238238/150‑1 (MQ=255)
                                                                        tttGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCttaa                                                                              <  2:1201109/150‑1 (MQ=255)
                                                                        tttGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCttaa                                                                              >  1:630059/1‑150 (MQ=255)
                                                                        tttGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCttaa                                                                              <  2:1273908/150‑1 (MQ=255)
                                                                            gTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACATCATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCttaattaa                                                                          <  2:1467277/150‑1 (MQ=255)
                                                                              tttCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTtaattaatt                                                                        >  1:1640122/1‑149 (MQ=255)
                                                                              tttCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATg                                                                        >  1:1042596/1‑150 (MQ=255)
                                                                              tttCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATg                                                                        >  1:1042581/1‑150 (MQ=255)
                                                                               ttCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGa                                                                       <  1:480688/150‑1 (MQ=255)
                                                                                  gATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACaa                                                                    >  1:1305107/1‑150 (MQ=255)
                                                                                  gATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACaa                                                                    >  1:1096832/1‑150 (MQ=255)
                                                                                  gATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCAATCTCTTTTCCTtaattaattaaaa                                                                    >  1:1317226/1‑145 (MQ=255)
                                                                                   aTACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAg                                                                   >  2:207131/1‑150 (MQ=255)
                                                                                   aTACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAg                                                                   <  1:1540319/150‑1 (MQ=255)
                                                                                   aTACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAg                                                                   <  1:846410/150‑1 (MQ=255)
                                                                                   aTACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAg                                                                   <  2:783730/150‑1 (MQ=255)
                                                                                     aCGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGt                                                                 <  1:835035/150‑1 (MQ=255)
                                                                                       gCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTaa                                                               >  1:127128/1‑150 (MQ=255)
                                                                                          ggCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAAt                                                            >  1:1207490/1‑150 (MQ=255)
                                                                                            cTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCa                                                          >  1:1156967/1‑150 (MQ=255)
                                                                                            cTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCa                                                          >  1:1214174/1‑150 (MQ=255)
                                                                                               gtggCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGc                                                       <  2:656166/150‑1 (MQ=255)
                                                                                               gtggCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGc                                                       >  1:1210238/1‑150 (MQ=255)
                                                                                                 ggCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGt                                                     <  1:53999/150‑1 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    >  1:743643/1‑150 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    <  1:1364971/150‑1 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    >  1:1700835/1‑150 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    <  1:1364771/150‑1 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    <  2:1310137/150‑1 (MQ=255)
                                                                                                  gCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGtt                                                    >  1:689121/1‑150 (MQ=255)
                                                                                                    tttGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTg                                                  <  1:1637250/150‑1 (MQ=255)
                                                                                                     ttGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTgg                                                 >  2:1721846/1‑150 (MQ=255)
                                                                                                     ttGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTgg                                                 >  2:1721850/1‑150 (MQ=255)
                                                                                                     ttGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTgg                                                 <  2:693874/150‑1 (MQ=255)
                                                                                                       gTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTc                                               <  2:80517/150‑1 (MQ=255)
                                                                                                       gTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTc                                               <  2:31089/150‑1 (MQ=255)
                                                                                                               cGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAATTTCAGGCGTTTGGTCGTGTCTTc                                       >  2:818584/1‑150 (MQ=255)
                                                                                                               cGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTc                                       <  1:1527681/150‑1 (MQ=255)
                                                                                                                 ttACACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCa                                     <  2:89797/150‑1 (MQ=255)
                                                                                                                 ttACACACAAGGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCa                                     <  2:781180/150‑1 (MQ=255)
                                                                                                                 ttAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCa                                     <  2:346609/150‑1 (MQ=255)
                                                                                                                 ttAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCa                                     <  2:69345/150‑1 (MQ=255)
                                                                                                                    aaCACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGAc                                  >  2:302764/1‑150 (MQ=255)
                                                                                                                    aaCACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTGCCAGAc                                  >  2:1224575/1‑150 (MQ=255)
                                                                                                                          ctGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTg                            <  1:1380295/149‑1 (MQ=255)
                                                                                                                          aTGCGTGTGCGATCTGGTTGCACCGCTTCATTGCAACCCTAGCTGATGGTTAACTACATTAAGGGTAATCCTGTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTTAAATCAGGCTTTTGGTCGTGTCTTCCAGACGTTCTg                            <  1:1622115/150‑1 (MQ=255)
                                                                                                                             cGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCa                         <  2:1451267/150‑1 (MQ=255)
                                                                                                                                gtgCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGAt                      <  2:791909/150‑1 (MQ=255)
                                                                                                                                gtgCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGAt                      <  2:127015/150‑1 (MQ=255)
                                                                                                                                 tgCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATg                     >  1:560616/1‑150 (MQ=255)
                                                                                                                                    gcttgggTTGCAATGCTGCGGTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGaat                  >  1:495335/5‑150 (MQ=255)
                                                                                                                                    gcttgggTTGCAATGCTGCGGTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGaat                  >  1:495348/5‑150 (MQ=255)
                                                                                                                                    gcttgggTTGCAATGCTGCGGTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGaat                  >  1:557985/5‑150 (MQ=255)
                                                                                                                                    gcttgggTTGCAATGCTGCGGTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGaat                  >  1:1418297/5‑150 (MQ=255)
                                                                                                                                          gTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCa            <  1:1533186/150‑1 (MQ=255)
                                                                                                                                          gTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCa            >  2:330998/1‑150 (MQ=255)
                                                                                                                                                ccGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTg      <  2:127128/150‑1 (MQ=255)
                                                                                                                                                 cGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTGc     <  2:785953/150‑1 (MQ=255)
                                                                                                                                                  gcagcaTTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTGCt    <  1:1504888/150‑1 (MQ=255)
                                                                                                                                                    agcaTTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTGCTCa  <  2:1473619/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCATTCTCTTTTCCTTAATTAATGACAAGGTAAAATCAGGCGTTTGGTCGTGTCTTCCAGACGTTCTGGCAGATGAATAATGCAGCTTTGCTCA  >  NC_000913/749500‑749797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: