Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,087,867 G→A 5.1% S664L (TCA→TTA)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,087,8670GA 5.1% 271.2 / 3.1 79S664L (TCA→TTA) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (42/32);  new base A (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTC  >  NC_000913/1087719‑1088016
                                                                                                                                                    |                                                                                                                                                     
aTGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGa                                                                                                                                                      >  2:1521581/1‑150 (MQ=255)
aTGGACATAAAAAACGGCCAGAAGAAAACGAACATCATCATCAGATCACCAGAGTGAAAGAACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGa                                                                                                                                                      >  1:1605125/1‑150 (MQ=255)
       tAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCa                                                                                                                                               >  2:1519176/1‑150 (MQ=255)
       tAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTAAAAACTCa                                                                                                                                               >  2:1096283/1‑150 (MQ=255)
       tAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTAAAAACTCa                                                                                                                                               >  2:1096254/1‑150 (MQ=255)
        aaaaaaCGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAg                                                                                                                                              <  1:1427205/150‑1 (MQ=255)
                  cAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAg                                                                                                                                    >  1:928835/1‑150 (MQ=255)
                  cAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAg                                                                                                                                    >  1:928852/1‑150 (MQ=255)
                      agaaAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCt                                                                                                                                >  1:1060994/1‑150 (MQ=255)
                          aaCGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTAttt                                                                                                                            >  1:221290/1‑150 (MQ=255)
                           aCGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTc                                                                                                                           >  1:644555/1‑150 (MQ=255)
                                ccTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggtt                                                                                                                      >  1:340249/1‑150 (MQ=255)
                                 cTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGTTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggttg                                                                                                                     <  2:1186812/150‑1 (MQ=255)
                                 cTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggttg                                                                                                                     <  2:1552016/150‑1 (MQ=255)
                                  tcatcatcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggttgg                                                                                                                    <  1:9883/150‑1 (MQ=255)
                                   catcatcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggttggt                                                                                                                   >  2:773057/1‑150 (MQ=255)
                                   catcatcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAggttggt                                                                                                                   <  2:155802/150‑1 (MQ=255)
                                     tcatcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCACACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTa                                                                                                                 <  2:600334/150‑1 (MQ=255)
                                        tcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGATTAAGGTCTATTTCAGGTTGGTTATGc                                                                                                              <  2:1034811/150‑1 (MQ=255)
                                        tcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGc                                                                                                              >  2:180172/1‑150 (MQ=255)
                                        tcaGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGc                                                                                                              <  2:162158/150‑1 (MQ=255)
                                           gTTCGCCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGa                                                                                                           >  1:366808/1‑150 (MQ=255)
                                           gTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGa                                                                                                           >  2:1245993/1‑150 (MQ=255)
                                           gTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGa                                                                                                           >  1:366815/1‑150 (MQ=255)
                                            ttCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGaa                                                                                                          <  2:1511756/150‑1 (MQ=255)
                                            ttCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGaa                                                                                                          <  2:1511751/150‑1 (MQ=255)
                                                         aaGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGata                                                                                             <  2:910838/150‑1 (MQ=255)
                                                            tacgctacgcATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGataata                                                                                          >  1:1472380/1‑150 (MQ=255)
                                                                             tATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTc                                                                         >  1:157729/1‑150 (MQ=255)
                                                                             tATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTc                                                                         >  1:940361/1‑150 (MQ=255)
                                                                                 cATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCAtt                                                                     >  1:487782/1‑150 (MQ=255)
                                                                                 cATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCAtt                                                                     >  1:971690/1‑150 (MQ=255)
                                                                                     acacCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGt                                                                 <  1:1532595/150‑1 (MQ=255)
                                                                                       acCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTg                                                               >  1:83506/1‑150 (MQ=255)
                                                                                       acCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTg                                                               >  1:923875/1‑150 (MQ=255)
                                                                                        ccAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGt                                                              >  2:359183/1‑150 (MQ=255)
                                                                                           gACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAAt                                                           >  1:1622556/1‑150 (MQ=255)
                                                                                            aCATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATa                                                          <  2:1622464/150‑1 (MQ=255)
                                                                                              ataataTAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAgg                                                        >  1:763719/1‑150 (MQ=255)
                                                                                                  tataAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCa                                                    >  1:680073/1‑150 (MQ=255)
                                                                                                  tataAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCa                                                    >  1:679937/1‑150 (MQ=255)
                                                                                                  tataAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCa                                                    >  1:539183/1‑150 (MQ=255)
                                                                                                      aaaaaCGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCa                                                <  2:273281/150‑1 (MQ=255)
                                                                                                      aaaaaCGATACGATGCGATTAATCATTTTTCGGAGACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCa                                                <  1:381980/150‑1 (MQ=255)
                                                                                                       aaaaCGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAg                                               <  1:505122/150‑1 (MQ=255)
                                                                                                       aaaaCGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAg                                               >  2:1148660/1‑150 (MQ=255)
                                                                                                        aaaCGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGt                                              <  2:221290/150‑1 (MQ=255)
                                                                                                        aaaCGAGACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGt                                              <  1:1377565/150‑1 (MQ=255)
                                                                                                                cgatGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAgt                                      <  1:451295/150‑1 (MQ=255)
                                                                                                                     cGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGttgttg                                 <  1:180172/150‑1 (MQ=255)
                                                                                                                        ttAATCATTTTTCGGATACCATGCTGTTTAAAACTCAGGACGAATAAGGTCTATTTCAGGTTTGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCga                              <  2:1203271/150‑1 (MQ=255)
                                                                                                                        ttAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCga                              <  2:646171/150‑1 (MQ=255)
                                                                                                                         tAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaa                             <  1:1519176/150‑1 (MQ=255)
                                                                                                                         tAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaa                             <  1:773057/150‑1 (MQ=255)
                                                                                                                         tAATCATTTTTCGGATACCAGGCTGTTAAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaa                             <  1:1096254/150‑1 (MQ=255)
                                                                                                                         tAATCATTTTTCGGATACCAGGCTGTTAAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaa                             <  1:1096283/150‑1 (MQ=255)
                                                                                                                          aaTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaag                            <  2:1060994/150‑1 (MQ=255)
                                                                                                                          aaTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaag                            >  2:1703657/1‑150 (MQ=255)
                                                                                                                           aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaaga                           >  1:1169945/1‑150 (MQ=255)
                                                                                                                           aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaaga                           >  1:1503128/1‑150 (MQ=255)
                                                                                                                           aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaaga                           >  1:806530/1‑150 (MQ=255)
                                                                                                                           aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaaga                           <  2:157729/150‑1 (MQ=255)
                                                                                                                           aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaaga                           >  1:19468/1‑150 (MQ=255)
                                                                                                                            tCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaagaa                          <  2:433479/150‑1 (MQ=255)
                                                                                                                            tCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaagaa                          >  2:374005/1‑150 (MQ=255)
                                                                                                                            tCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCgaagaa                          >  2:373899/1‑150 (MQ=255)
                                                                                                                               tttttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATa                       <  2:644555/150‑1 (MQ=255)
                                                                                                                                 tttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGc                     >  2:1506155/1‑150 (MQ=255)
                                                                                                                                 tttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGc                     >  2:994999/1‑150 (MQ=255)
                                                                                                                                        tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGc              >  1:1547067/1‑150 (MQ=255)
                                                                                                                                        tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGc              >  1:997800/1‑150 (MQ=255)
                                                                                                                                           cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCGGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGa           <  2:246228/150‑1 (MQ=255)
                                                                                                                                           cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGa           >  1:155760/1‑150 (MQ=255)
                                                                                                                                           cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGa           <  1:67495/150‑1 (MQ=255)
                                                                                                                                           cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGa           <  2:1300932/150‑1 (MQ=255)
                                                                                                                                           cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATGCAGTGGTAATAGGCGAATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGa           >  2:828374/1‑150 (MQ=255)
                                                                                                                                              gCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCa        >  1:1112269/1‑150 (MQ=255)
                                                                                                                                              gCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCa        <  2:1385731/150‑1 (MQ=255)
                                                                                                                                                 gTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCAttt     <  1:1694079/150‑1 (MQ=255)
                                                                                                                                                    gAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTc  <  1:38980/150‑1 (MQ=255)
                                                                                                                                                    gAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTc  >  2:1222154/1‑150 (MQ=255)
                                                                                                                                                    gAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTc  >  2:1222155/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGGACATAAAAAACGGCCAGAAGAAAACGAACCTCATCATCAGTTCACCAGAGTGAAAGTACGCTACGCATAGGGGTATGCATAACACCAGACATAATATAAAAAACGATACGATGCGATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTC  >  NC_000913/1087719‑1088016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: