Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,219,312 T→C 7.8% intergenic (+111/‑289) ymgF → / → ycgH inner membrane division septum protein/pseudogene; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,219,3120TC 7.8% 245.1 / 8.7 80intergenic (+111/‑289)ymgF/ycgHinner membrane division septum protein/pseudogene; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (39/33);  new base C (3/3);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

TTAAGAGAAAGACTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGCCGACTTATTT  >  NC_000913/1219163‑1219460
                                                                                                                                                     |                                                                                                                                                    
ttAAGAGAAAGACTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTAt                                                                                                                                                      >  2:157563/1‑150 (MQ=255)
    gagaAAGACTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACg                                                                                                                                                  <  2:946467/150‑1 (MQ=255)
       aaaGACTGGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGgtt                                                                                                                                               <  2:1412929/150‑1 (MQ=255)
           aCTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTGTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGttt                                                                                                                                           <  2:1175885/150‑1 (MQ=255)
            cTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGtttt                                                                                                                                          <  1:696530/150‑1 (MQ=255)
            cTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGtttt                                                                                                                                          <  1:1520741/150‑1 (MQ=255)
             tCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGttttt                                                                                                                                         <  1:1466265/150‑1 (MQ=255)
              cGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTta                                                                                                                                        <  1:1317726/150‑1 (MQ=255)
               gTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTtat                                                                                                                                       <  1:977374/150‑1 (MQ=255)
               gTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTtat                                                                                                                                       <  1:977390/150‑1 (MQ=255)
               gTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTtat                                                                                                                                       <  1:333012/150‑1 (MQ=255)
                     cacaGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCa                                                                                                                                 >  1:1284146/1‑150 (MQ=255)
                     cacaGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCa                                                                                                                                 >  1:1556558/1‑150 (MQ=255)
                     cacaGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCa                                                                                                                                 >  2:1698099/1‑150 (MQ=255)
                       caGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCAcc                                                                                                                               >  1:1608250/1‑150 (MQ=255)
                       caGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCAcc                                                                                                                               >  1:340554/1‑150 (MQ=255)
                       caGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCAcc                                                                                                                               >  2:1084454/1‑150 (MQ=255)
                       caGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCAcc                                                                                                                               >  2:1084456/1‑150 (MQ=255)
                       caGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCAcc                                                                                                                               >  2:1084487/1‑150 (MQ=255)
                          ggATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGaa                                                                                                                            >  2:1288057/1‑150 (MQ=255)
                               aGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAaataat                                                                                                                       <  1:607305/150‑1 (MQ=255)
                                    taatCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCa                                                                                                                  >  2:506030/1‑150 (MQ=255)
                                         tttCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCa                                                                                                             >  1:1116800/1‑150 (MQ=255)
                                         tttCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCa                                                                                                             >  2:1375979/1‑150 (MQ=255)
                                         tttCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCa                                                                                                             >  2:1375987/1‑150 (MQ=255)
                                         tttCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCa                                                                                                             >  2:1376029/1‑150 (MQ=255)
                                         tttCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCa                                                                                                             >  2:400409/1‑150 (MQ=255)
                                            cAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACt                                                                                                          >  2:494090/1‑150 (MQ=255)
                                              gTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAt                                                                                                        <  1:1164861/150‑1 (MQ=255)
                                              gTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAt                                                                                                        <  1:539909/150‑1 (MQ=255)
                                              gTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAt                                                                                                        <  1:843391/150‑1 (MQ=255)
                                              gTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAt                                                                                                        <  1:821041/150‑1 (MQ=255)
                                               tttCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAtt                                                                                                       >  2:1454513/1‑150 (MQ=255)
                                               tttCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTAtt                                                                                                       >  2:1454515/1‑150 (MQ=255)
                                                 tctcACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTaa                                                                                                     <  2:1556558/150‑1 (MQ=255)
                                                  ctcACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAAc                                                                                                    >  1:1392303/1‑150 (MQ=255)
                                                  ctcACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAAc                                                                                                    >  1:1257611/1‑150 (MQ=255)
                                                      cTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGTTCATAGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCATGGTGTTAGCCCGGTTGTTTTTATATATCACCGAAAGAATCCTCATCGCGCCTATTAACAAtt                                                                                                >  2:734971/1‑150 (MQ=255)
                                                       tGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAAttt                                                                                               >  1:660484/1‑150 (MQ=255)
                                                        gTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAAtttt                                                                                              <  2:1122707/150‑1 (MQ=255)
                                                                  ggCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAag                                                                                    >  1:1199774/1‑150 (MQ=255)
                                                                            gTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGt                                                                          >  1:586895/1‑150 (MQ=255)
                                                                             ttGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGtt                                                                         <  1:1104199/150‑1 (MQ=255)
                                                                             ttGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGtt                                                                         >  1:1560978/1‑150 (MQ=255)
                                                                             ttGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGtt                                                                         >  1:1560968/1‑150 (MQ=255)
                                                                             ttGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGtt                                                                         <  1:1104224/150‑1 (MQ=255)
                                                                                cTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTaaa                                                                      <  1:1691226/150‑1 (MQ=255)
                                                                                  ggCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACa                                                                    >  1:1590342/1‑150 (MQ=255)
                                                                                   gCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACaa                                                                   >  1:22688/1‑150 (MQ=255)
                                                                                     aGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACaaaa                                                                 >  1:866763/1‑150 (MQ=255)
                                                                                       tGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATc                                                               >  2:1021278/1‑150 (MQ=255)
                                                                                       tGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATACTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATc                                                               >  2:1212843/1‑150 (MQ=255)
                                                                                         aaCGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGt                                                             <  1:566498/150‑1 (MQ=255)
                                                                                            gCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCAc                                                          >  1:487905/1‑150 (MQ=255)
                                                                                            gCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAAAAAAATCGTCAc                                                          >  1:646199/1‑150 (MQ=255)
                                                                                              cTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCt                                                        >  2:80601/1‑150 (MQ=255)
                                                                                              cTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCt                                                        >  2:20694/1‑150 (MQ=255)
                                                                                                  aTCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCaaa                                                    <  1:1422121/150‑1 (MQ=255)
                                                                                                  aTCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCaaa                                                    <  1:187845/150‑1 (MQ=255)
                                                                                                  aTCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCaaa                                                    <  1:181249/150‑1 (MQ=255)
                                                                                                      ttGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGTTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATAGTCACCTCAAAGTGa                                                >  2:145393/1‑150 (MQ=255)
                                                                                                      ttGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGa                                                >  2:145305/1‑150 (MQ=255)
                                                                                                       tGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGAt                                               >  2:1070503/1‑150 (MQ=255)
                                                                                                          aGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCaa                                            >  1:1059612/1‑150 (MQ=255)
                                                                                                                 gCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATg                                     >  2:295051/1‑150 (MQ=255)
                                                                                                                     tagtGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGaaaa                                 <  2:1724380/147‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  2:1608250/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  1:547663/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  1:759564/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  2:340554/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  1:1084454/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  1:1084456/150‑1 (MQ=255)
                                                                                                                         gcgcAAATAGTTGTCAACCCTGGTGTTACCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAg                             <  1:1084487/150‑1 (MQ=255)
                                                                                                                          cgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAgg                            >  2:217172/1‑150 (MQ=255)
                                                                                                                          cgcAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAgg                            >  2:308684/1‑150 (MQ=255)
                                                                                                                                          cccTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGc            <  2:1392303/150‑1 (MQ=255)
                                                                                                                                          cccTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGc            <  2:1257611/150‑1 (MQ=255)
                                                                                                                                          cccTGCTGATAACACGGATGTATTTATATAACACCTAAATAATCCTCATCGCAACTAATAACAATTTTCATCTCGAAGAGTTAATTGATAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGc            <  2:1705945/150‑1 (MQ=255)
                                                                                                                                               gtgtTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGTTAATATGTTGTAAATTGATAATGcgtagt       >  2:1588617/1‑145 (MQ=255)
                                                                                                                                                    aTCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGCCGACttattt  <  2:1560978/150‑1 (MQ=255)
                                                                                                                                                    aTCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGCCGACttattt  <  2:1560968/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTAAGAGAAAGACTCGTCAACCACAGGGATCAGCAATAATCTTTCAGTTTCTCACTGTCAGTATGCGGCTGAATGGGTTGCTGGCAGTGAACGCCTGGATCATTGAAGGAAAGGCATTATTGCGCAAATAGTTGTCAACCCTGGTGTTATCACGGTTGTTTTTATATATCACCGAAATAATCCTCATCGCAACTATTAACAATTTTGATGTCGAAGAGTTATTTGTTAAACAAAATCGTCACCTCAAAGTGATCAATGTCATGAAAATAAGGTGAAAAATGATAATGCCGACTTATTT  >  NC_000913/1219163‑1219460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: