Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,598,529 A→T 5.0% intergenic (‑542/+88) yneO ← / ← lsrK pseudogene, AidA homolog/autoinducer‑2 (AI‑2) kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,598,5290AT 5.0% 354.1 / 7.8 99intergenic (‑542/+88)yneO/lsrKpseudogene, AidA homolog/autoinducer‑2 (AI‑2) kinase
Reads supporting (aligned to +/- strand):  ref base A (42/52);  new base T (2/3);  total (44/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 101 total aligned reads displayed.

AAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGC  >  NC_000913/1598381‑1598674
                                                                                                                                                    |                                                                                                                                                 
aaGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTaa                                                                                                                                                  <  2:142759/150‑1 (MQ=255)
aaGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTaa                                                                                                                                                  <  2:981625/150‑1 (MQ=255)
   tttATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACaa                                                                                                                                               <  2:895965/150‑1 (MQ=255)
     tattaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGc                                                                                                                                             <  2:298412/150‑1 (MQ=255)
     taataAACCCCTTTTGCATACATTAGTGACTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGc                                                                                                                                             <  2:1269742/147‑1 (MQ=255)
       ttaAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCa                                                                                                                                           <  1:78935/150‑1 (MQ=255)
           aCCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTGTTAAAAGCCAGctt                                                                                                                                       <  1:211564/150‑2 (MQ=255)
           aCCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTGTTAAAAGCCAGctt                                                                                                                                       <  1:211611/150‑2 (MQ=255)
           aCCCCATTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTGTTAAAAGCCAGctt                                                                                                                                       <  1:211569/150‑2 (MQ=255)
            ccccTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGctct                                                                                                                                      >  1:1697933/1‑150 (MQ=255)
            ccccTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGctct                                                                                                                                      >  1:164645/1‑150 (MQ=255)
              ccTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaa                                                                                                                                    >  2:1054406/1‑150 (MQ=255)
               cTTTTGCATCCATTAGTGTCTTTATGAATATTCGCTATAAAATAAGGGTTGTGCTTAATTCTTTATGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaaa                                                                                                                                   <  1:1203279/150‑1 (MQ=255)
               cTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaaa                                                                                                                                   <  1:1073147/150‑1 (MQ=255)
                ttttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaaaa                                                                                                                                  <  2:559355/150‑1 (MQ=255)
                ttttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTaaaa                                                                                                                                  <  1:890220/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  1:568310/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  2:342701/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  1:1653753/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  1:1552792/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  1:227959/150‑1 (MQ=255)
                  ttGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaaga                                                                                                                                <  1:887768/150‑1 (MQ=255)
                   tGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAaagaa                                                                                                                               <  1:1190009/150‑1 (MQ=255)
                     cATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAgg                                                                                                                             <  1:446970/150‑1 (MQ=255)
                     cATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAgg                                                                                                                             <  1:446888/150‑1 (MQ=255)
                      aTCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAggg                                                                                                                            <  2:1348361/150‑1 (MQ=255)
                       tCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGa                                                                                                                           >  2:894899/1‑150 (MQ=255)
                          aTTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                                                                                        >  2:1100351/1‑150 (MQ=255)
                          aTTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAAt                                                                                                                        <  2:977038/150‑1 (MQ=255)
                           ttAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGTAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATa                                                                                                                       >  1:722809/1‑150 (MQ=255)
                                  cTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATaa                                                                                                                <  1:464213/150‑1 (MQ=255)
                                  cTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATaa                                                                                                                <  1:464258/150‑1 (MQ=255)
                                   tttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAAc                                                                                                               <  2:1481127/150‑1 (MQ=255)
                                    ttAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACt                                                                                                              >  2:698937/1‑150 (MQ=255)
                                     tAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACta                                                                                                             >  1:633415/1‑150 (MQ=255)
                                        gAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATAc                                                                                                          >  2:814474/1‑150 (MQ=255)
                                                gCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAAc                                                                                                  >  1:1111959/1‑150 (MQ=255)
                                                   ataAAATAAGTGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTTGATTTTAACAAGCCAGCTCTAAAAGAAGGTAAATAAGAATAACTATACTCAAAAACTaa                                                                                               <  2:1704917/150‑1 (MQ=255)
                                                    taAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAAc                                                                                              >  1:426658/1‑150 (MQ=255)
                                                    taAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAAc                                                                                              >  1:426638/1‑150 (MQ=255)
                                                      aaaTAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAg                                                                                            >  1:1090366/1‑150 (MQ=255)
                                                      aaaTAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAg                                                                                            >  1:1090363/1‑150 (MQ=255)
                                                      aaaTAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAg                                                                                            >  2:243041/1‑150 (MQ=255)
                                                                  tgCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                                                                                >  1:1620232/1‑150 (MQ=255)
                                                                  tgCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                                                                                >  1:1354660/1‑150 (MQ=255)
                                                                  tgCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                                                                                >  1:1354680/1‑150 (MQ=255)
                                                                  tgCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGACAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGtcatca                                                                                >  1:170368/1‑150 (MQ=255)
                                                                     ttAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCatga                                                                             >  1:1075107/1‑150 (MQ=255)
                                                                      tAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCatgat                                                                            >  1:558239/1‑150 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  1:671337/150‑1 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  2:1496564/150‑1 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  1:698937/150‑1 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  2:1115032/150‑1 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  2:1115083/150‑1 (MQ=255)
                                                                          gCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTgg                                                                        <  1:405991/150‑1 (MQ=255)
                                                                              tAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGGCATCATGATGTGGCTGt                                                                    >  2:1236138/1‑150 (MQ=255)
                                                                                  aaaaaTAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAAt                                                                <  1:158667/150‑1 (MQ=255)
                                                                                  aaaaaTAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAAt                                                                <  1:158679/150‑1 (MQ=255)
                                                                                   aaaaTAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATg                                                               >  1:660588/1‑150 (MQ=255)
                                                                                     aaTAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGaa                                                             <  1:268927/150‑1 (MQ=255)
                                                                                      aTAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGaaa                                                            <  1:243041/150‑1 (MQ=255)
                                                                                          cAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACtat                                                        >  1:477136/1‑150 (MQ=255)
                                                                                           aaTTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAGGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCGGTCAATGTAAGtatg                                                       >  2:1364639/1‑149 (MQ=255)
                                                                                              tttCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAcc                                                    <  2:99602/150‑1 (MQ=255)
                                                                                              tttCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAcc                                                    <  2:99598/150‑1 (MQ=255)
                                                                                              tttCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAcc                                                    <  1:655052/150‑1 (MQ=255)
                                                                                                  ccTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTCTAACCCAgg                                                >  2:349907/1‑150 (MQ=255)
                                                                                                   cTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgc                                               <  1:176537/150‑1 (MQ=255)
                                                                                                   cTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgc                                               <  1:176530/150‑1 (MQ=255)
                                                                                                    ttGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgcg                                              >  2:1615300/1‑150 (MQ=255)
                                                                                                         tatCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                         <  1:814474/150‑1 (MQ=255)
                                                                                                            cGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                      <  1:487873/150‑1 (MQ=255)
                                                                                                            cGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                      <  1:1638189/150‑1 (MQ=255)
                                                                                                            cGTACGGGTGATGGAACGATGAATCTGCAATCTGGCTTTTAACAAGACAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                      <  1:487912/150‑1 (MQ=255)
                                                                                                                 tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacga                                 >  2:1471664/1‑150 (MQ=255)
                                                                                                                     gATGGAACGATGAATCTGCAAGCTGGCTTTTAACACGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCGGTCCATAACGAcgtc                             <  2:660588/150‑1 (MQ=255)
                                                                                                                     gATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtc                             <  2:558239/150‑1 (MQ=255)
                                                                                                                       tGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtcgt                           >  2:127350/1‑150 (MQ=255)
                                                                                                                       tGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCGTTCCATAACGAcgtcgt                           <  2:1278817/150‑1 (MQ=255)
                                                                                                                        ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACgtcgtc                          >  1:596539/1‑150 (MQ=255)
                                                                                                                            cGATGAATCTGTAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTc                      <  2:722809/150‑1 (MQ=255)
                                                                                                                                gAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATg                  >  2:30300/1‑150 (MQ=255)
                                                                                                                                  aTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAt                >  2:27521/1‑150 (MQ=255)
                                                                                                                                      gCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAc            >  2:630426/1‑150 (MQ=255)
                                                                                                                                      gCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAc            >  1:596950/1‑150 (MQ=255)
                                                                                                                                      gCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAc            >  1:476666/1‑150 (MQ=255)
                                                                                                                                      gCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAc            >  1:880125/1‑150 (MQ=255)
                                                                                                                                      gCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAc            >  1:111917/1‑150 (MQ=255)
                                                                                                                                        gaGCTGGCTTGTTAAAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCa          >  1:72588/2‑150 (MQ=255)
                                                                                                                                         aGCTGGCTTGTTAAAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAg         >  1:897064/1‑150 (MQ=255)
                                                                                                                                            tgtcTTTTAACAAGCCATCTCTAAAAGAAGGTAAATAAGAATTACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTTTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGccc      <  2:110387/147‑1 (MQ=255)
                                                                                                                                             ggCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGcccc     <  1:1004080/150‑1 (MQ=255)
                                                                                                                                              gCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCa    <  2:408645/150‑1 (MQ=255)
                                                                                                                                              gCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCa    <  2:408603/150‑1 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGTGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  >  1:1452760/1‑150 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  >  1:67626/1‑150 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  >  1:67643/1‑150 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  >  1:67733/1‑150 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  <  2:1472217/150‑1 (MQ=255)
                                                                                                                                                ttttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGc  >  1:67754/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAGTTTATTAAACCCCTTTTGCATCCATTAGTGTCTTTAGGAATATTCGCTATAAAATAAGGGTTGTGCTTAATGCTTTAAGAAAAATAGCAATTTTCCCTTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGC  >  NC_000913/1598381‑1598674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: