Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,062,355 T→G 6.4% intergenic (+20/+36) asnV → / ← ldtA tRNA‑Asn/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,062,3550TG 6.4% 388.2 / 14.5 110intergenic (+20/+36)asnV/ldtAtRNA‑Asn/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base T (42/61);  new base G (5/2);  total (47/63)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 112 total aligned reads displayed.

ACCCTTTGACATCACCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATAAACGCCCGCA  >  NC_000913/2062208‑2062503
                                                                                                                                                   |                                                                                                                                                    
aCCCTTTGACATCACCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCt                                                                                                                                                    >  1:316263/1‑150 (MQ=255)
        aCATCACCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTAGAAAGCAGGCt                                                                                                                                            <  1:1660357/150‑1 (MQ=255)
        aCATCACCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTAGAAAGCAGGCt                                                                                                                                            <  1:785911/150‑1 (MQ=255)
             aCCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTg                                                                                                                                       <  2:127411/150‑1 (MQ=255)
             aCCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTg                                                                                                                                       <  1:1364475/150‑1 (MQ=255)
             aCCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGACCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTg                                                                                                                                       <  1:365416/150‑1 (MQ=255)
               cctGCACTGCCATTAATCTGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCt                                                                                                                                     <  2:1416148/148‑1 (MQ=255)
               cATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGTCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCt                                                                                                                                     <  2:1112792/150‑1 (MQ=255)
               cATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCt                                                                                                                                     <  2:414661/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  2:177430/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  2:177410/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  2:1552611/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  1:1477873/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  1:1085638/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  1:465597/150‑1 (MQ=255)
                 tGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCttt                                                                                                                                   <  2:450548/150‑1 (MQ=255)
                     cTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTaa                                                                                                                               <  2:371949/150‑1 (MQ=255)
                        ccATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTa                                                                                                                            >  1:1033780/1‑150 (MQ=255)
                         cATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTAc                                                                                                                           <  1:1643429/150‑1 (MQ=255)
                           ttAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCa                                                                                                                         >  1:175437/1‑150 (MQ=255)
                              atatGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACg                                                                                                                      >  1:42843/1‑150 (MQ=255)
                                    gcCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTa                                                                                                                >  1:89819/1‑150 (MQ=255)
                                    gcCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCATGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACAAAAGCTCTTa                                                                                                                >  1:675982/1‑150 (MQ=255)
                                       ccGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTaaaa                                                                                                             <  1:629032/150‑1 (MQ=255)
                                          ttCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACAt                                                                                                          >  1:364067/1‑150 (MQ=255)
                                          ttCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACAt                                                                                                          <  1:37015/150‑1 (MQ=255)
                                           tCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATc                                                                                                         <  2:204831/150‑1 (MQ=255)
                                            cacaCTATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCt                                                                                                        <  2:1406718/150‑1 (MQ=255)
                                               acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGGGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTc                                                                                                     >  2:736281/1‑150 (MQ=255)
                                               acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTc                                                                                                     >  1:655848/1‑150 (MQ=255)
                                               acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTc                                                                                                     >  2:736373/1‑150 (MQ=255)
                                               acGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTc                                                                                                     >  2:736372/1‑150 (MQ=255)
                                                  aTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTg                                                                                                  <  2:1182871/150‑1 (MQ=255)
                                                       tctGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCa                                                                                             >  1:827357/1‑150 (MQ=255)
                                                          ggaGTTCAGTCGGGAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCACCGCTCTTAAAACATCTGTCTTGAACCAGaa                                                                                          <  1:453388/148‑1 (MQ=255)
                                                          gTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGaa                                                                                          <  2:457328/150‑1 (MQ=255)
                                                              ttCAGTCGGTAGAACGGCTGACTGTGAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTaa                                                                                      <  2:600288/150‑1 (MQ=255)
                                                                  gTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTg                                                                                  >  2:1664463/1‑150 (MQ=255)
                                                                   tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGc                                                                                 >  1:742940/1‑150 (MQ=255)
                                                                   tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATATGc                                                                                 >  1:1598456/1‑150 (MQ=255)
                                                                       tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAg                                                                             <  2:344993/150‑1 (MQ=255)
                                                                       tAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAg                                                                             <  2:488021/150‑1 (MQ=255)
                                                                        aGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTATCAGGCTTTTTGCTTTCTTTTTACCACCGCTCTTAAAACATCTGTCTTGAACCAGATCTAATTTGCACAgg                                                                            <  2:821376/150‑1 (MQ=37)
                                                                           aCGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCAt                                                                         <  2:1033780/150‑1 (MQ=255)
                                                                            cggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCAtt                                                                        <  2:651286/150‑1 (MQ=255)
                                                                             ggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAg                                                                             <  2:589211/144‑1 (MQ=255)
                                                                             ggcggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAg                                                                             >  1:589211/1‑144 (MQ=255)
                                                                               cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTccc                                                                     <  1:1465866/150‑1 (MQ=255)
                                                                               cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTccc                                                                     >  1:1478850/1‑150 (MQ=255)
                                                                               cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTccc                                                                     >  1:1478914/1‑150 (MQ=255)
                                                                               cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTccc                                                                     >  1:1478925/1‑150 (MQ=255)
                                                                                  aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGAt                                                                  <  1:176693/150‑1 (MQ=255)
                                                                                    tGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCg                                                                >  2:621955/1‑150 (MQ=255)
                                                                                     gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGa                                                               >  2:187306/1‑150 (MQ=255)
                                                                                     gTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAAGCATTCCCGATCGa                                                               >  1:40499/1‑150 (MQ=255)
                                                                                      ttAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGAc                                                              <  2:192513/150‑1 (MQ=255)
                                                                                         aTCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGtt                                                           >  1:1155277/1‑150 (MQ=255)
                                                                                          tCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTg                                                          <  2:639774/150‑1 (MQ=255)
                                                                                            cgaaTGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCa                                                        <  2:149105/147‑1 (MQ=255)
                                                                                            cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCa                                                        <  2:870366/150‑1 (MQ=255)
                                                                                            cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCa                                                        <  2:1275314/150‑1 (MQ=255)
                                                                                            cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTATTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCa                                                        <  2:1021840/150‑1 (MQ=255)
                                                                                             gTATGTCACTGGTTCGAGTACCGTCAGAGGTGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCaa                                                       <  2:478965/150‑1 (MQ=255)
                                                                                                tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACgc                                                    >  1:1181272/1‑150 (MQ=255)
                                                                                                tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCAGTCCCGATCGACGTTGCAACgc                                                    >  1:1181297/1‑150 (MQ=255)
                                                                                                     cTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCAt                                               >  1:273500/1‑150 (MQ=255)
                                                                                                          tCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCAAAGGCATTCCCGATCGACGTTGCAACGCAGCATTTgcg                                          >  2:472740/1‑150 (MQ=255)
                                                                                                               tCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGAtt                                     >  1:819917/1‑150 (MQ=255)
                                                                                                               tCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGAtt                                     <  1:453995/150‑1 (MQ=255)
                                                                                                               tCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGAtt                                     >  1:1690633/1‑150 (MQ=255)
                                                                                                                 cAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTa                                   >  1:512824/1‑150 (MQ=255)
                                                                                                                 cAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTa                                   >  1:1373866/1‑150 (MQ=255)
                                                                                                                  aGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTAc                                  <  1:202677/150‑1 (MQ=255)
                                                                                                                  aGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTAc                                  <  1:1436664/150‑1 (MQ=255)
                                                                                                                   gTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACa                                 >  1:330853/1‑150 (MQ=255)
                                                                                                                   gTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACa                                 >  1:330899/1‑150 (MQ=255)
                                                                                                                    tCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACAt                                <  1:1426631/150‑1 (MQ=255)
                                                                                                                     cAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATc                               <  1:241502/150‑1 (MQ=255)
                                                                                                                     cAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATc                               <  2:81676/150‑1 (MQ=255)
                                                                                                                       gaggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCaa                             <  1:1399346/150‑1 (MQ=255)
                                                                                                                       gaggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCaa                             <  2:1410538/150‑1 (MQ=255)
                                                                                                                       gaggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCaa                             <  1:549224/150‑1 (MQ=255)
                                                                                                                       gaggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCaa                             <  1:972751/150‑1 (MQ=255)
                                                                                                                       gaggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCaa                             <  1:977660/150‑1 (MQ=255)
                                                                                                                         ggagCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGACCTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAAct                           >  1:1010222/1‑150 (MQ=255)
                                                                                                                              cAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTg                      <  2:293975/150‑1 (MQ=255)
                                                                                                                              cAAATACAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTg                      <  2:933789/150‑1 (MQ=255)
                                                                                                                               aaaTTCAAAAAAGCCTGCTTTCTAGCAGGCTGTGTGCTTTCTAATTACCAACGCTCTTAAAACAGCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGAGCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGc                     >  2:1174082/1‑150 (MQ=255)
                                                                                                                                 aaagcaaaaaGCCTGCTAGAAAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGGACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGccc                   >  1:1084247/6‑150 (MQ=255)
                                                                                                                                  ttCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCg                  >  1:834992/1‑150 (MQ=255)
                                                                                                                                  aagcaaaaaGCCTGCTAGAAAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCg                  >  2:1507156/5‑150 (MQ=255)
                                                                                                                                   tCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGt                 <  2:1153618/150‑1 (MQ=255)
                                                                                                                                    cAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATGACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGtt                <  1:301297/150‑1 (MQ=255)
                                                                                                                                      aaaaaGCCTGCTAGAAAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGa              >  2:638614/1‑150 (MQ=255)
                                                                                                                                      aaaaaGCCTGCTAGAAAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGa              >  2:646901/1‑150 (MQ=255)
                                                                                                                                         aaGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATaa           <  2:42843/150‑1 (MQ=255)
                                                                                                                                         aaGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATCTGCGCGATTTACATCAACTTCTTGCCCGTTGATaa           >  1:781262/1‑150 (MQ=255)
                                                                                                                                             cTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATAAACGc       <  2:1624174/150‑1 (MQ=255)
                                                                                                                                               gCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATAAACGccc     <  2:827357/150‑1 (MQ=255)
                                                                                                                                                  ttCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATAAACGCCCGCa  >  2:510079/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACCCTTTGACATCACCATGCACTGCCATTAATATGCGCCCCGTTCACACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCAAAAAAGCCTGCTTTCTAGCAGGCTTTTTGCTTTCTAATTACCAACGCTCTTAAAACATCTGTCTTGAACCAGAACTAATTTGCACAGGCATTCCCGATCGACGTTGCAACGCAGCATTTGCGCGATTTACATCAACTTCTTGCCCGTTGATAAACGCCCGCA  >  NC_000913/2062208‑2062503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: