Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 17.3% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA17.3% 204.1 / 8.7 97intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (33/42);  new base A (9/11);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383
                                                                                                                                                    |                                                                                                                                                     
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      <  1:209044/150‑1 (MQ=255)
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      >  1:1267101/1‑150 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                   <  1:1674291/150‑1 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                   <  1:908402/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:406040/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAatat                                                                                                                                                >  1:760276/1‑150 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  1:1080233/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               >  2:752274/1‑150 (MQ=255)
               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       >  2:1123693/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     <  2:1327220/150‑1 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     <  2:461614/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:1008710/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:1008698/150‑1 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATtttttttt                                                                                                                                >  1:1606988/1‑150 (MQ=255)
                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAAAATGAAATTATTTGttttt                                                                                                                             >  1:1551704/1‑150 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  2:50407/150‑1 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAATAAATTATTTTTTTTTaaa                                                                                                                          >  1:346283/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                          <  2:592514/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  2:23203/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTCAAAATATAATTAAATTATTTTTTTTTAAaaat                                                                                                                       >  1:187270/1‑150 (MQ=255)
                               tgtCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  2:1089636/148‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:451716/150‑1 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                  <  2:765525/150‑1 (MQ=255)
                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                               <  1:1474752/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATaaag                                                                                                             >  1:619516/1‑148 (MQ=255)
                                                ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  1:826338/150‑1 (MQ=255)
                                                  gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                    <  1:971117/150‑1 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:1192998/150‑1 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:443922/150‑1 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:443930/150‑1 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCATGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATGGAGATAACGATCACAAAAAcca                                                                                                >  1:1630182/1‑148 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                               <  2:860350/150‑1 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                               <  2:860356/150‑1 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAATAAATTAATTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACaa                                                                                             >  1:909212/1‑150 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACaa                                                                                             >  2:1510639/1‑150 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGGGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTGTTCAATATCGCGGTAACGATCACAAAAACGACac                                                                                             >  1:1292152/1‑149 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAAAAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATAACAAAAACCACAAAATGaa                                                                                       >  2:1026924/1‑150 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                      <  2:1623522/150‑1 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATTTAATTATTTTTTTTTAAATTTAGATATAAAGATAACAAAAAAGACAatatttaa                                                                                      >  2:207806/1‑146 (MQ=255)
                                                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTATAAATATAAAGAAATAATATTTTTTTAAAATACAATAGAACGAACAAAAAAACGACAACATGtaaa                                                                                     >  1:1444083/1‑150 (MQ=255)
                                                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTAAAAATAAAATGTAATTATTTTTTTTTTATTATGGGGATAACGATTAAACAAAAGACCAAATGgaaa                                                                                     >  1:1228855/1‑150 (MQ=255)
                                                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAAAATAATGTAAATATTTTTTTTTTAATATAGAGATAAAGATCACAAAAAACACAAAATGaaaa                                                                                     >  1:585351/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAATATTTTTTTTTTAAAATAGGGATAACGATAAAAAAAAAGACAAAAAGAAAat                                                                                    >  1:1489471/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTc                                                                               >  2:1549720/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               >  2:1549692/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAAGACAATATGAAAattattg                                                                               >  1:1116864/1‑149 (MQ=255)
                                                                        ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAAAATGTAAATTATTCg                                                                              >  2:565354/1‑150 (MQ=255)
                                                                          gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATAAAATGAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAACACAATATGAAAAATATTagag                                                                            >  1:1098099/1‑150 (MQ=255)
                                                                            gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:1427878/150‑1 (MQ=255)
                                                                                 gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATTTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:646737/150‑1 (MQ=255)
                                                                                 gCGTTGTTCAGCACCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:1362681/150‑1 (MQ=255)
                                                                                  cttttttCAGAATCTTCTTTTTCGATACCACTTAATTTTTAATATCGTTTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  1:1614370/145‑1 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACCAAATGAAAATTATTAGAGGAGTGaaa                                                                    >  2:1670917/1‑150 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATTAAAATAATTTTTTTTAAATATAGAGATAACCATAAAAAAAACGACAAAAAGAAAATTATTCGAGGAGTGaaa                                                                    >  2:1670909/1‑150 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAAAATAAATAAATTTTTTTTTTTGATATATTGTGATAACGATAACCAAAAACACAAAATTTAAATTATTCGAGGGGTGGAAGGcccaa                                                             >  1:1719841/1‑146 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAAAAATAAATTATTTGTTTTTTAATATAGAAATAAAGATAAAAAAAAACACAATAATTAAATTATTAGGGGGGTGGAAAGCaaaa                                                             >  1:1290443/1‑150 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                            >  2:1726721/1‑150 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                            >  2:532886/1‑150 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAAATAAATTTTTTTTTTTTTAATTTATAGATAAACGTAACAAAAAACACAATATTAAAAATATTAGCTGAGTGTTAGGGaaaaa                                                            >  1:346621/1‑150 (MQ=255)
                                                                                            aaTCTTCTTTGTCGATACCACTTAAATTTCCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATGAAATTTTTTTTTTTTAAATATCGATATAACGAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:95719/149‑1 (MQ=255)
                                                                                            aaTCGGCTTTTTCTATACCACTTTCTTTTTCATTTCCTGTCTTTTTTTTTCGATTTCAAAATATTTTTAATTTTTTTTTTTTTAAATATCTATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:1464699/149‑1 (MQ=255)
                                                                                             aTCTGGTTTGTAGATCCCACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:1163842/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCTACACCAGGTAATTTTAAATTTCCTGTTTTTTGTTTTCTTTTAAAAAATATAATGAAATTTTTTGTTTTAAAATATCTAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:752294/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCAATTAATTTTTCATATCCTGTCGTTTTTTTTATTTTTAAAAATATATTTAAATTTTTTTTTTTTAAATATCGATATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:752439/150‑1 (MQ=255)
                                                                                              aatGCTTTGTCGTTACCACGTACTTTTTCATATCCTTTGGTTTTTTTTCTATTTCAAAATTTAATGAAATTTTTTTTTTTTAAATATCTTTATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                        <  2:592639/148‑1 (MQ=255)
                                                                                                       tCGATACCACTTACATTTTCATATCCTTTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:1083745/150‑1 (MQ=255)
                                                                                                       tCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:755048/150‑1 (MQ=255)
                                                                                                       tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:1083835/150‑1 (MQ=255)
                                                                                                           tACCACTTAAATTTTCATATCCTGTCTTTTGTTTTCGATTAAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  1:942536/150‑1 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCGATAGGGa                                         >  2:1484591/1‑150 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAAAGGGa                                         >  2:672114/1‑150 (MQ=255)
                                                                                                              cACTTACATTTTATTATCCTGTGTTTTTTTTTCGATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCTAGATAACGATAAAAAAAACTACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:1144804/150‑1 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  2:1370567/150‑1 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  2:578467/150‑1 (MQ=255)
                                                                                                              cACGTAAATTTTCATATCCTGTCTTTTGTTTTCTATTAAAAAATATATTGAAATTTTTTTTTTTTAAATATCTATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:1423029/150‑1 (MQ=255)
                                                                                                              cACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATATTGAATTTATTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:1144788/150‑1 (MQ=255)
                                                                                                               aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAg                                       >  2:255889/1‑150 (MQ=255)
                                                                                                                cttACTTTTACTTATCTTGTCTTTTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATATCTATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                      <  1:1404960/148‑1 (MQ=255)
                                                                                                                cGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATCATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                      <  2:1725395/150‑1 (MQ=255)
                                                                                                                    attttaCATATCCTTTGTTTTTTTTTCTTTTAAAAAATATATTAAATTTTTTTTTTTTAAAATATCTATATCCCCATCACAACACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:640769/148‑1 (MQ=255)
                                                                                                                    aaTTTTACTATCCTGGCGTTTTTTTTCTTTTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:640795/149‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                 <  2:1489471/150‑1 (MQ=255)
                                                                                                                               ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:694452/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAAAGTGcgcg                      >  2:252188/1‑150 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAAAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      >  2:252187/1‑150 (MQ=255)
                                                                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:1258264/1‑150 (MQ=255)
                                                                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:1183232/1‑150 (MQ=255)
                                                                                                                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   >  2:1684502/1‑150 (MQ=255)
                                                                                                                                         tttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:639028/149‑1 (MQ=255)
                                                                                                                                         tttttCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:868325/149‑1 (MQ=255)
                                                                                                                                           tttCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:1559146/150‑1 (MQ=255)
                                                                                                                                           tttCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:1559143/150‑1 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGCCGGa       >  2:1538582/1‑150 (MQ=255)
                                                                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     >  1:164516/1‑150 (MQ=255)
                                                                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     >  1:1323189/1‑150 (MQ=255)
                                                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    >  1:606173/1‑150 (MQ=255)
                                                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    >  1:606169/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  1:1505106/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: