Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 16.3% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA16.3% 218.3 / 4.9 93intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (30/44);  new base A (10/8);  total (41/52)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGC  >  NC_000913/2177134‑2177425
                                                                                                                                                 |                                                                                                                                                  
ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                                                <  1:826338/150‑1 (MQ=255)
  gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                                                              <  1:971117/150‑1 (MQ=255)
   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                             <  1:1192998/150‑1 (MQ=255)
      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                          <  2:443930/150‑1 (MQ=255)
      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                          <  2:443922/150‑1 (MQ=255)
      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCATGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATGGAGATAACGATCACAAAAAcca                                                                                                                                          >  1:1630182/1‑148 (MQ=255)
       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                         <  2:860350/150‑1 (MQ=255)
       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                         <  2:860356/150‑1 (MQ=255)
         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAATAAATTAATTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACaa                                                                                                                                       >  1:909212/1‑150 (MQ=255)
         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACaa                                                                                                                                       >  2:1510639/1‑150 (MQ=255)
         ggAACCGCATAACCGCCGCGCTGGGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTGTTCAATATCGCGGTAACGATCACAAAAACGACac                                                                                                                                       >  1:1292152/1‑149 (MQ=255)
               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAAAAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATAACAAAAACCACAAAATGaa                                                                                                                                 >  2:1026924/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                                                <  2:1623522/150‑1 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATTTAATTATTTTTTTTTAAATTTAGATATAAAGATAACAAAAAAGACAatatttaa                                                                                                                                >  2:207806/1‑146 (MQ=255)
                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTATAAATATAAAGAAATAATATTTTTTTAAAATACAATAGAACGAACAAAAAAACGACAACATGtaaa                                                                                                                               >  1:1444083/1‑150 (MQ=255)
                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTAAAAATAAAATGTAATTATTTTTTTTTTATTATGGGGATAACGATTAAACAAAAGACCAAATGgaaa                                                                                                                               >  1:1228855/1‑150 (MQ=255)
                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAAAATAATGTAAATATTTTTTTTTTAATATAGAGATAAAGATCACAAAAAACACAAAATGaaaa                                                                                                                               >  1:585351/1‑150 (MQ=255)
                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAATATTTTTTTTTTAAAATAGGGATAACGATAAAAAAAAAGACAAAAAGAAAat                                                                                                                              >  1:1489471/1‑150 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTc                                                                                                                         >  2:1549720/1‑150 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                                                         >  2:1549692/1‑150 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAAGACAATATGAAAattattg                                                                                                                         >  1:1116864/1‑149 (MQ=255)
                        ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAAAATGTAAATTATTCg                                                                                                                        >  2:565354/1‑150 (MQ=255)
                          gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATAAAATGAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAACACAATATGAAAAATATTagag                                                                                                                      >  1:1098099/1‑150 (MQ=255)
                            gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                                                    <  2:1427878/150‑1 (MQ=255)
                                 gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATTTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                                                               <  2:646737/150‑1 (MQ=255)
                                 gCGTTGTTCAGCACCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                                                               <  2:1362681/150‑1 (MQ=255)
                                  cttttttCAGAATCTTCTTTTTCGATACCACTTAATTTTTAATATCGTTTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                              <  1:1614370/145‑1 (MQ=255)
                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACCAAATGAAAATTATTAGAGGAGTGaaa                                                                                                              >  2:1670917/1‑150 (MQ=255)
                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATTAAAATAATTTTTTTTAAATATAGAGATAACCATAAAAAAAACGACAAAAAGAAAATTATTCGAGGAGTGaaa                                                                                                              >  2:1670909/1‑150 (MQ=255)
                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAAAATAAATAAATTTTTTTTTTTGATATATTGTGATAACGATAACCAAAAACACAAAATTTAAATTATTCGAGGGGTGGAAGGcccaa                                                                                                       >  1:1719841/1‑146 (MQ=255)
                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAAAAATAAATTATTTGTTTTTTAATATAGAAATAAAGATAAAAAAAAACACAATAATTAAATTATTAGGGGGGTGGAAAGCaaaa                                                                                                       >  1:1290443/1‑150 (MQ=255)
                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                                                                      >  2:1726721/1‑150 (MQ=255)
                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                                                                      >  2:532886/1‑150 (MQ=255)
                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAAATAAATTTTTTTTTTTTTAATTTATAGATAAACGTAACAAAAAACACAATATTAAAAATATTAGCTGAGTGTTAGGGaaaaa                                                                                                      >  1:346621/1‑150 (MQ=255)
                                            aaTCTTCTTTGTCGATACCACTTAAATTTCCATATCCTGTCTTTTTTTTTCTTTTTAAAAATATAATGAAATTTTTTTTTTTTAAATATCGATATAACGAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                    <  1:95719/149‑1 (MQ=255)
                                            aaTCGGCTTTTTCTATACCACTTTCTTTTTCATTTCCTGTCTTTTTTTTTCGATTTCAAAATATTTTTAATTTTTTTTTTTTTAAATATCTATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                    <  1:1464699/149‑1 (MQ=255)
                                             aTCTGGTTTGTAGATCCCACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                   <  1:1163842/150‑1 (MQ=255)
                                             aTCTGCTTTGTCTACACCAGGTAATTTTAAATTTCCTGTTTTTTGTTTTCTTTTAAAAAATATAATGAAATTTTTTGTTTTAAAATATCTAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                   <  1:752294/150‑1 (MQ=255)
                                             aTCTGCTTTGTCGATACCAATTAATTTTTCATATCCTGTCGTTTTTTTTATTTTTAAAAATATATTTAAATTTTTTTTTTTTAAATATCGATATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                   <  1:752439/150‑1 (MQ=255)
                                              aatGCTTTGTCGTTACCACGTACTTTTTCATATCCTTTGGTTTTTTTTCTATTTCAAAATTTAATGAAATTTTTTTTTTTTAAATATCTTTATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                                                  <  2:592639/148‑1 (MQ=255)
                                                       tCGATACCACTTACATTTTCATATCCTTTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                         <  2:1083745/150‑1 (MQ=255)
                                                       tCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                         <  2:755048/150‑1 (MQ=255)
                                                       tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                         <  2:1083835/150‑1 (MQ=255)
                                                           tACCACTTAAATTTTCATATCCTGTCTTTTGTTTTCGATTAAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                                                     <  1:942536/150‑1 (MQ=255)
                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCGATAGGGa                                                                                   >  2:1484591/1‑150 (MQ=255)
                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAAAGGGa                                                                                   >  2:672114/1‑150 (MQ=255)
                                                              cACTTACATTTTATTATCCTGTGTTTTTTTTTCGATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCTAGATAACGATAAAAAAAACTACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                  <  1:1144804/150‑1 (MQ=255)
                                                              cACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                  <  2:1370567/150‑1 (MQ=255)
                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                  <  2:578467/150‑1 (MQ=255)
                                                              cACGTAAATTTTCATATCCTGTCTTTTGTTTTCTATTAAAAAATATATTGAAATTTTTTTTTTTTAAATATCTATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                  <  1:1423029/150‑1 (MQ=255)
                                                              cACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATATTGAATTTATTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                                                  <  1:1144788/150‑1 (MQ=255)
                                                               aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAg                                                                                 >  2:255889/1‑150 (MQ=255)
                                                                cttACTTTTACTTATCTTGTCTTTTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATATCTATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                                                <  1:1404960/148‑1 (MQ=255)
                                                                cGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATCATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                                                <  2:1725395/150‑1 (MQ=255)
                                                                    attttaCATATCCTTTGTTTTTTTTTCTTTTAAAAAATATATTAAATTTTTTTTTTTTAAAATATCTATATCCCCATCACAACACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                            <  1:640769/148‑1 (MQ=255)
                                                                    aaTTTTACTATCCTGGCGTTTTTTTTCTTTTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                            <  1:640795/149‑1 (MQ=255)
                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                                                           <  2:1489471/150‑1 (MQ=255)
                                                                               ccTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                 <  1:694452/150‑1 (MQ=255)
                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAAAGTGcgcg                                                                >  2:252188/1‑150 (MQ=255)
                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAAAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                                                                >  2:252187/1‑150 (MQ=255)
                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                               >  2:1258264/1‑150 (MQ=255)
                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                               >  2:1183232/1‑150 (MQ=255)
                                                                                   tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                                                             >  2:1684502/1‑150 (MQ=255)
                                                                                         tttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                       <  1:639028/149‑1 (MQ=255)
                                                                                         tttttCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                       <  1:868325/149‑1 (MQ=255)
                                                                                           tttCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                     <  1:1559146/150‑1 (MQ=255)
                                                                                           tttCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                     <  1:1559143/150‑1 (MQ=255)
                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGCCGGa                                                 >  2:1538582/1‑150 (MQ=255)
                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                               >  1:1323189/1‑150 (MQ=255)
                                                                                                 tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                                               >  1:164516/1‑150 (MQ=255)
                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                              >  1:606169/1‑150 (MQ=255)
                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc                                              >  1:606173/1‑150 (MQ=255)
                                                                                                    cAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                            <  1:1505106/150‑1 (MQ=255)
                                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                           >  1:807832/1‑150 (MQ=255)
                                                                                                       aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                         <  1:1123693/150‑1 (MQ=255)
                                                                                                         tataATTAATTTATTTGTTTTAAAATATAGATATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                       <  1:629877/150‑1 (MQ=255)
                                                                                                         tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                       <  1:1236790/150‑1 (MQ=255)
                                                                                                         tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                       <  1:1236738/150‑1 (MQ=255)
                                                                                                         tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                       <  2:1098099/150‑1 (MQ=255)
                                                                                                              tGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                  <  2:187270/150‑1 (MQ=255)
                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                  >  2:1508766/1‑150 (MQ=255)
                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                  >  2:55407/1‑150 (MQ=255)
                                                                                                                 aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                               <  2:1399454/150‑1 (MQ=255)
                                                                                                                 aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                               <  2:1399427/150‑1 (MQ=255)
                                                                                                                   ttattTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                             <  1:565354/150‑1 (MQ=255)
                                                                                                                        tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                        <  2:1116864/150‑1 (MQ=255)
                                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                     >  2:1710390/1‑150 (MQ=255)
                                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                     >  2:857875/1‑150 (MQ=255)
                                                                                                                                   atCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGCGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa             <  2:346283/150‑1 (MQ=255)
                                                                                                                                         aTAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa       <  2:1553068/150‑1 (MQ=255)
                                                                                                                                         aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa       <  2:1553008/150‑1 (MQ=255)
                                                                                                                                         aTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATaa       <  2:1553071/150‑1 (MQ=255)
                                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc  >  2:274311/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGC  >  NC_000913/2177134‑2177425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: