Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,632,584 A→T 5.1% intergenic (‑49/+20) guaA ← / ← guaB GMP synthetase (glutamine aminotransferase)/IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,632,5840AT 5.1% 410.0 / 7.4 117intergenic (‑49/+20)guaA/guaBGMP synthetase (glutamine aminotransferase)/IMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (50/61);  new base T (4/2);  total (54/63)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 118 total aligned reads displayed.

AGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAG  >  NC_000913/2632436‑2632733
                                                                                                                                                    |                                                                                                                                                     
aGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACAt                                                                                                                                                      >  1:337291/1‑150 (MQ=255)
aGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACAt                                                                                                                                                      >  1:337304/1‑150 (MQ=255)
    aaCACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAg                                                                                                                                                  >  2:371731/1‑150 (MQ=255)
     acacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGt                                                                                                                                                 >  2:524301/1‑150 (MQ=255)
     acacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGt                                                                                                                                                 >  2:134014/1‑150 (MQ=255)
     acacCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGt                                                                                                                                                 >  2:134016/1‑150 (MQ=255)
       acCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCg                                                                                                                                               >  1:1217717/1‑150 (MQ=255)
       acCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCg                                                                                                                                               >  1:351532/1‑150 (MQ=255)
         ccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCTAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCggg                                                                                                                                             <  1:777247/150‑1 (MQ=255)
         ccAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACTTCATGTCggg                                                                                                                                             <  2:709840/150‑1 (MQ=255)
                acgcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAagag                                                                                                                                      >  2:1158967/1‑150 (MQ=255)
                acgcacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAagag                                                                                                                                      >  2:337528/1‑150 (MQ=255)
                   cacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                                                                   <  2:251708/150‑1 (MQ=255)
                   cacgcGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAAt                                                                                                                                   <  2:251726/150‑1 (MQ=255)
                    acgcGGCGCGCAACCTTTTGTGTTTACTGAGAACCGAAGTCCATATTTAGGATGCGATGCTTATGAATGTTTTCCGTCTTTGACGCTTATTCCGAGGCAAGTTAAACATATAATATAAATCGCCCGACATGATGTCGGGCGAAGAGAATc                                                                                                                                  <  1:1725385/150‑1 (MQ=255)
                     cgcgGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCa                                                                                                                                 <  2:1362956/150‑1 (MQ=255)
                        ggcgCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGa                                                                                                                              <  2:839290/150‑1 (MQ=255)
                                     ttGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAg                                                                                                                 >  1:774611/1‑150 (MQ=255)
                                       gAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGtt                                                                                                               <  2:513484/150‑1 (MQ=255)
                                           gtACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                                                                           <  1:1623571/150‑1 (MQ=255)
                                           gtACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                                                                           <  1:153678/150‑1 (MQ=255)
                                           gtACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCggg                                                                                                           <  1:1417504/150‑1 (MQ=255)
                                            tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                          >  1:601004/1‑150 (MQ=255)
                                            tACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCgggg                                                                                                          >  1:875370/1‑150 (MQ=255)
                                                gagaACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGActc                                                                                                      >  1:1577208/1‑150 (MQ=255)
                                                  gaACCGAAGTCCCGACTGAGGATGCGATGCTTATGACTGTTTTCCGTCATTGACGCTTATTCCGCGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCtt                                                                                                    <  1:218035/150‑1 (MQ=255)
                                                    aCCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTa                                                                                                  <  2:1227816/150‑1 (MQ=255)
                                                         aGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAAt                                                                                             <  2:1339027/150‑1 (MQ=255)
                                                         aGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAAt                                                                                             <  2:1339039/150‑1 (MQ=255)
                                                         aGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAAt                                                                                             <  2:1371197/150‑1 (MQ=255)
                                                          gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                            <  1:1567094/150‑1 (MQ=255)
                                                          gTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATg                                                                                            <  1:1518399/150‑1 (MQ=255)
                                                              aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                                                                        >  2:828768/1‑150 (MQ=255)
                                                              aGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCa                                                                                        <  1:447817/150‑1 (MQ=255)
                                                               gAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAc                                                                                       >  2:40575/1‑150 (MQ=255)
                                                               gAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAc                                                                                       >  2:248737/1‑150 (MQ=255)
                                                                  tGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtc                                                                                    <  1:236711/150‑1 (MQ=255)
                                                                    aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                                  >  1:1489398/1‑150 (MQ=255)
                                                                    aGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCAcgtcgt                                                                                  <  1:1585999/150‑1 (MQ=255)
                                                                      gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                <  1:634313/150‑1 (MQ=255)
                                                                      gatgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGa                                                                                >  1:1603722/1‑150 (MQ=255)
                                                                        tgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                                                                              <  2:351532/150‑1 (MQ=255)
                                                                        tgcgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAAc                                                                              <  2:1217717/150‑1 (MQ=255)
                                                                          cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTACTGGTCACGTCGTGAACGt                                                                            <  1:1039514/150‑1 (MQ=255)
                                                                          cgatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGt                                                                            <  1:1608093/150‑1 (MQ=255)
                                                                           gatgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTg                                                                           >  2:1429266/1‑150 (MQ=255)
                                                                            atgcTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTgg                                                                          <  1:1708821/150‑1 (MQ=255)
                                                                               cTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCtt                                                                       >  1:308662/1‑150 (MQ=255)
                                                                                 tATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATACATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTc                                                                     <  1:368655/150‑1 (MQ=255)
                                                                                  aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCTTGAACGTGGCTTTcc                                                                    <  2:1465042/150‑1 (MQ=255)
                                                                                  aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTcc                                                                    <  2:607115/150‑1 (MQ=255)
                                                                                  aTGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTcc                                                                    <  2:1413953/150‑1 (MQ=255)
                                                                                     aaTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGa                                                                 >  1:1710119/1‑150 (MQ=255)
                                                                                      aTGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGaa                                                                <  1:1312376/150‑1 (MQ=255)
                                                                                            tCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCg                                                          <  1:78277/150‑1 (MQ=255)
                                                                                              cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGATTGCCCGct                                                        >  2:665179/1‑149 (MQ=255)
                                                                                              cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCa                                                        >  2:527620/1‑150 (MQ=255)
                                                                                              cGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCa                                                        >  1:1709385/1‑150 (MQ=255)
                                                                                                tCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCAcc                                                      >  2:1625183/1‑150 (MQ=255)
                                                                                                   ttGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCt                                                   >  1:1508652/1‑150 (MQ=255)
                                                                                                   ttGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCt                                                   >  1:263991/1‑150 (MQ=255)
                                                                                                   ttGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCt                                                   >  1:1016283/1‑150 (MQ=255)
                                                                                                    tGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTg                                                  >  2:1252311/1‑150 (MQ=255)
                                                                                                       cGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATa                                               <  2:774611/150‑1 (MQ=255)
                                                                                                        gCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                                              <  2:826943/150‑1 (MQ=255)
                                                                                                        gCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATAc                                              <  2:827106/150‑1 (MQ=255)
                                                                                                          ttATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGt                                            >  1:925397/1‑150 (MQ=255)
                                                                                                          ttATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGt                                            >  2:880620/1‑150 (MQ=255)
                                                                                                               ccGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACaaa                                       >  1:1494709/1‑150 (MQ=255)
                                                                                                                 gAGGCAAGTGAAACAGATACTATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGACCGTGGCTTTCCTGACTGCCCGCACCGCTGATACGTACAAACt                                     >  2:30364/1‑150 (MQ=255)
                                                                                                                       aGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCtt                               >  1:1036142/1‑150 (MQ=255)
                                                                                                                       aGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCtt                               >  1:494094/1‑150 (MQ=255)
                                                                                                                         tGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTa                             >  2:356398/1‑150 (MQ=255)
                                                                                                                           aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGTACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           <  2:1650558/150‑1 (MQ=255)
                                                                                                                           aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           <  2:609166/150‑1 (MQ=255)
                                                                                                                           aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           <  2:156389/150‑1 (MQ=255)
                                                                                                                           aaaCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGt                           <  2:571609/150‑1 (MQ=255)
                                                                                                                             aCAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTAc                         <  2:523628/150‑1 (MQ=255)
                                                                                                                               aGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                        <  2:225240/149‑1 (MQ=255)
                                                                                                                               aGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACg                        >  1:225240/1‑149 (MQ=255)
                                                                                                                                gATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCa                      <  2:337291/150‑1 (MQ=255)
                                                                                                                                gATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCa                      <  2:857547/150‑1 (MQ=255)
                                                                                                                                gATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCa                      <  2:875370/150‑1 (MQ=255)
                                                                                                                                  taataTAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGt                    <  2:623335/150‑1 (MQ=255)
                                                                                                                                      ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt                <  1:200848/150‑1 (MQ=255)
                                                                                                                                      ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt                <  1:200859/150‑1 (MQ=255)
                                                                                                                                      ataAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTtcgt                >  1:1162773/1‑150 (MQ=255)
                                                                                                                                              cccGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        >  2:875022/1‑150 (MQ=255)
                                                                                                                                              cccGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        >  2:898914/1‑150 (MQ=255)
                                                                                                                                              cccGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        >  2:1505241/1‑150 (MQ=255)
                                                                                                                                              cccGACTTCATGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTGTAGTACGCAGTTCGTCGATAGTa        >  2:268342/1‑150 (MQ=255)
                                                                                                                                              cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        >  2:1163152/1‑150 (MQ=255)
                                                                                                                                              cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        <  2:543711/150‑1 (MQ=255)
                                                                                                                                              cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        <  2:543642/150‑1 (MQ=255)
                                                                                                                                              cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTa        <  2:1724520/150‑1 (MQ=255)
                                                                                                                                              cccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCGTTAGTACGCAGTTCGTCGAGAGTa        >  2:1163205/1‑150 (MQ=255)
                                                                                                                                               ccGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTAc       >  1:73358/1‑150 (MQ=255)
                                                                                                                                                 gACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACca     >  2:1725470/1‑150 (MQ=255)
                                                                                                                                                  aCATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACATACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACcac    >  1:461337/1‑150 (MQ=255)
                                                                                                                                                    aTGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAg  >  1:1490456/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTAAACACCCAGCTCACGCACGCGGCGCGCAACCAGTTGAGTGTACTGAGAACCGAAGTCCAGAATGAGGATGCGATGCTTATGAATGTTTTCCGTCATTGACGCTTATTCCGAGGCAAGTGAAACAGATAATATAAATCGCCCGACATGAAGTCGGGCGAAGAGAATCAGGAGCCCAGACGGTAGTTCGGGGACTCTTTAGTAATGGTCACGTCGTGAACGTGGCTTTCCTGAATGCCCGCACCGCTGATACGTACAAACTCCGCTTTAGTACGCAGTTCGTCGATAGTACCACAG  >  NC_000913/2632436‑2632733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: