Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 360,266 T→G 5.2% L92R (CTT→CGT)  cynX → putative cyanate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913360,2660TG 5.2% 338.3 / 5.6 100L92R (CTT→CGT) cynXputative cyanate transporter
Reads supporting (aligned to +/- strand):  ref base T (62/31);  new base G (4/2);  total (67/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCA  >  NC_000913/360118‑360415
                                                                                                                                                    |                                                                                                                                                     
ctGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCtt                                                                                                                                                      <  1:1481697/150‑1 (MQ=255)
   ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTagc                                                                                                                                                   >  1:1428194/1‑150 (MQ=255)
   ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTagc                                                                                                                                                   >  1:133239/1‑150 (MQ=255)
   ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGATGCTTagc                                                                                                                                                   >  1:1428012/1‑150 (MQ=255)
   ccGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCAGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTagc                                                                                                                                                   >  1:1023902/1‑150 (MQ=255)
   ccGGTGGTAACCATGGGCCGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGGGAGCTTTACCCGCAAAGTGCGCTGCTGCTTagc                                                                                                                                                   >  2:1044965/1‑150 (MQ=255)
            aCCTTGGGCGGGCTGGGGCTGGCCGGAAGCTGGCTTCATCAGCTTGTCTGCGAAGGTGGCAGTGTCGCCTTCAGTGTGTTGCTGATTGCGGTGGGTGCTTTGATGCGTTCGCTTTACCCGCAATGTGCGCTGCTAAGCAGCAGCGCACtt                                                                                                                                          <  1:940538/150‑2 (MQ=255)
             ccATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGc                                                                                                                                         >  1:1313589/1‑150 (MQ=255)
               aTGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTAAGCAGCAGCGCACtttgc                                                                                                                                       <  2:179697/150‑5 (MQ=255)
                  ggcgggcTGGCGCTGGCCGGAAGCAGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGAATGCCGTCGGTGCATTGATGAGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCAtggt                                                                                                                                    >  1:762740/1‑150 (MQ=255)
                   gcgggcTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGACCGTCGCAGTGTCGCCATCAGTCTGTAGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTAAGCAGCGCACTGCTgggtg                                                                                                                                   >  2:815819/1‑150 (MQ=255)
                       gctggcgcgggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGTTTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTggtggggt                                                                                                                               <  2:183855/150‑1 (MQ=255)
                        ctggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTggtgggggg                                                                                                                              >  1:1612002/1‑148 (MQ=255)
                        ctggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCGTAGCAGCGCACTGCTTggtgggggg                                                                                                                              >  1:1612005/1‑148 (MQ=255)
                          ggcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGgtggggtggg                                                                                                                            <  2:830425/150‑1 (MQ=255)
                           gcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGtggggtgggg                                                                                                                           >  2:131666/1‑150 (MQ=255)
                           gcgctggcCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGtggggtgggg                                                                                                                           >  2:549576/1‑150 (MQ=255)
                                  ccGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGca                                                                                                                    >  1:494826/1‑150 (MQ=255)
                                  ccGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGca                                                                                                                    >  2:40601/1‑150 (MQ=255)
                                  ccGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGca                                                                                                                    >  1:494654/1‑150 (MQ=255)
                                   cGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcat                                                                                                                   >  1:557238/1‑150 (MQ=255)
                                   cGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcat                                                                                                                   >  1:1232840/1‑150 (MQ=255)
                                   cGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcat                                                                                                                   >  1:897929/1‑150 (MQ=255)
                                   cGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcat                                                                                                                   >  2:1623508/1‑150 (MQ=255)
                                    ggAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGcatc                                                                                                                  >  1:558125/1‑150 (MQ=255)
                                       cgCTGGGTTCATCAGCATGTCCGCGAACGCCGCCGTGTCGCCATCTGTCTGTTGCTGATTGCGGTGGGTGCTTGGAGGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCCGCGCACTGCTTGGTGGGGTGGGGATCGGCATCAtt                                                                                                               <  2:832743/149‑1 (MQ=255)
                                            gCTTCATCAGCTTGTCAGCGACCGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGTATTGAGGCGGGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCCGGc                                                                                                          <  2:554292/150‑1 (MQ=255)
                                                  tcaGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGAt                                                                                                    >  1:1534893/1‑150 (MQ=255)
                                                  tcaGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGAt                                                                                                    >  1:1425589/1‑150 (MQ=255)
                                                  tcaGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCGTGGGGGGGTGGGGATCGGCATCATTCAGGCGGTGAt                                                                                                    >  1:677959/1‑150 (MQ=255)
                                                      cATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCt                                                                                                >  2:790902/1‑150 (MQ=255)
                                                      cATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCGTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCt                                                                                                >  2:172623/1‑150 (MQ=255)
                                                          tCAGCGAACGTCGCCGTGTCGCCATCTGTCTGTTGCTTTTTGCGGTGGGTGCATTTTTGCGTTAGTTTTCCCCGAAACGGGGGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCgg                                                                                            <  1:90356/150‑1 (MQ=255)
                                                          tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCgg                                                                                            <  2:942601/150‑1 (MQ=255)
                                                          tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCgg                                                                                            <  2:942663/150‑1 (MQ=255)
                                                           ctgCGAAGGTCGCTGTGTCGCCATCCGTCTGTCGCTGTTTGCCGTCGGTGCATTGATGCGTGTGCTTTCCCCGCAACGTGGGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGt                                                                                           <  2:1026382/148‑1 (MQ=255)
                                                           cggctaaCGTCGCCGTGGCGGCATCTGTGTGTTTCTGTTTGCCGGCGGTGCATTGATGCGTGAGGTTTTCCCGCATTGTGCTCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGt                                                                                           <  2:1026366/145‑1 (MQ=255)
                                                              cGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGAt                                                                                        >  2:716830/1‑150 (MQ=255)
                                                                    tCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAATGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcg                                                                                  >  1:973578/1‑150 (MQ=255)
                                                                      gctgtgtCGCCATCTGTGTGTGGCTGTTTGCCGTGGTTGCATTTTTGCGTTAGATTTACCCGCAATGTGCGCTGGTGCTTTGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggc                                                                                <  2:762740/147‑1 (MQ=255)
                                                                       cAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggcg                                                                               <  2:1261314/150‑1 (MQ=255)
                                                                       cAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggcg                                                                               >  2:956841/1‑150 (MQ=255)
                                                                              gCCATCAGTCTGTTGCTGTTTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcag                                                                        <  2:1363898/150‑1 (MQ=255)
                                                                                cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagca                                                                      >  1:1057252/1‑150 (MQ=255)
                                                                                cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagca                                                                      >  1:562318/1‑150 (MQ=255)
                                                                                cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCAGTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagca                                                                      >  2:1105307/1‑150 (MQ=255)
                                                                                cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTCGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagca                                                                      >  2:139992/1‑150 (MQ=255)
                                                                                cATCAGTCTGTTGCTGATTGCAGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCGTGGTGGGGGGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGGGTcagcc                                                                      >  1:453723/1‑149 (MQ=255)
                                                                                 aTCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACGGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagcag                                                                     >  1:1321471/1‑150 (MQ=255)
                                                                                 aTCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCATAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagcag                                                                     >  1:1598289/1‑150 (MQ=255)
                                                                                     gTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCa                                                                 >  2:1506341/1‑150 (MQ=255)
                                                                                     gTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCa                                                                 >  1:349399/1‑150 (MQ=255)
                                                                                     gTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGAGTAAACGGCGGTTTCAGCAGCGCa                                                                 >  2:1506348/1‑150 (MQ=255)
                                                                                      tCTGTTGCTTTTTGCCGTGGGTGATTGGATGCGTGAGCTTCACCCCAAATGGGCGCTGCTGCTTAGCCGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCAc                                                                <  1:892107/150‑1 (MQ=255)
                                                                                      tCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCAc                                                                >  2:234010/1‑150 (MQ=255)
                                                                                        tGTTGCTGTTTGCCGTCGGTGCATTGTTGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGc                                                              <  2:1219692/150‑1 (MQ=255)
                                                                                          tggCTGATTGCCGTCGGTGCATTGAGGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTCGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCa                                                            <  2:383210/148‑1 (MQ=255)
                                                                                           tGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCAc                                                           >  1:1194411/1‑150 (MQ=255)
                                                                                            gCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                                                          >  1:237119/1‑150 (MQ=255)
                                                                                            gCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                                                          >  1:1104709/1‑150 (MQ=255)
                                                                                            gCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCAGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                                                          >  1:1107220/1‑150 (MQ=255)
                                                                                             cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTg                                                         >  2:407632/1‑150 (MQ=255)
                                                                                             cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTg                                                         >  1:1361865/1‑150 (MQ=255)
                                                                                             cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTg                                                         >  2:212168/1‑150 (MQ=255)
                                                                                                aTTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTg                                                      >  1:1020962/1‑150 (MQ=255)
                                                                                                      gTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATgggg                                                >  2:1702516/1‑150 (MQ=255)
                                                                                                      gTCGGTGCATTGATGCGTGAGCTTTACCAGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTAGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATgggg                                                >  1:622732/1‑150 (MQ=255)
                                                                                                         ggTGCATTGTTGGGTGAGCTTTACCCGCAAAGTGGGGTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtg                                             <  1:787584/150‑1 (MQ=255)
                                                                                                         ggTGAATTGATGCGTGTGTTTTACCCCCAACGGGCGCTGCTGATTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtg                                             <  1:621164/150‑1 (MQ=255)
                                                                                                          gTGCATTGATGCGTGAGCTTTACCCGCAACGTGCGCTGCTGCTTAGCCGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgt                                            <  1:678759/150‑1 (MQ=255)
                                                                                                          gTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCCCTGGTGATGGGGCtgt                                            >  1:308611/1‑150 (MQ=255)
                                                                                                          gTGCATTGATGCGTGAGCTTAACCCGCAAAGTGCGCTGCTGCTTAGCCGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgt                                            <  1:678744/150‑1 (MQ=255)
                                                                                                             cATTGTTGCGTGAGCTTTACCCGCAACGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTATACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgtcag                                         <  2:1624447/150‑4 (MQ=255)
                                                                                                             cATTGTTGCGTGAGATTTACCCGCAATGTGCGCTGCGGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgtcag                                         <  2:1624615/150‑4 (MQ=255)
                                                                                                              aTTGAGGCGTGCGCTTTCCCCGCAAAGGGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTc                                        <  1:1334467/150‑1 (MQ=255)
                                                                                                                  aTGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCcgcg                                    <  2:558125/150‑1 (MQ=255)
                                                                                                                    gCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGc                                  >  2:330946/1‑150 (MQ=255)
                                                                                                                         aGCTTTACCCGCAATGTGCGCTGCTGCTTTGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGa                             <  1:1614530/150‑1 (MQ=255)
                                                                                                                         aGCTTTACCCGCAATGTGCGCTGCTGCTTAGCTGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGa                             <  1:1144172/150‑1 (MQ=255)
                                                                                                                          gCTTTACCCGCAAAGTGCGGTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGAt                            <  2:786318/150‑1 (MQ=255)
                                                                                                                          gCTTTACCCGCAAAGTGCGCTGATGCTTATCAGCGCACTGCGTGGTGGGGTGGGGATCGGCAAACATCACGCGGTGTTTCCTTAGGTGATTAAAAGGCGGTTTAAGCAGCGCACGCCACGGGTGATGGGGCTGTGGTCCGCGGCTCAGAt                            >  1:1613485/1‑150 (MQ=255)
                                                                                                                             ttACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATggg                         >  1:1000464/1‑150 (MQ=255)
                                                                                                                              tACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGggc                        <  2:1399127/150‑1 (MQ=255)
                                                                                                                              tACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGggc                        <  2:1399132/150‑1 (MQ=255)
                                                                                                                                accaagcaGTGCGCTGCTAAGCAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGggcgg                      >  2:1710171/8‑150 (MQ=255)
                                                                                                                                  cGCAAAGGGCGCTGCTGCGTAGCAGCGCACTGCTTGGGGGGGTGGGGATCGGCATCCTTCAGGCGGTGATGCCTTCGGTGATGAAACGGCGGGTTCAGCAGCGCACGCCACTGGTGCTGGGGCTGTGGTCCGCGGCGCTGAGGGgcggcg                    >  2:1074907/1‑150 (MQ=255)
                                                                                                                                   gCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGcggcgg                   <  1:275345/150‑1 (MQ=255)
                                                                                                                                         tGCGCTGCTAAGCAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCt             >  2:119021/1‑150 (MQ=255)
                                                                                                                                           cgcTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTg           >  2:1704810/1‑150 (MQ=255)
                                                                                                                                           cgcTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTg           >  2:1704747/1‑150 (MQ=255)
                                                                                                                                           cgcTGCGGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTg           <  1:1309256/150‑1 (MQ=255)
                                                                                                                                             ctgctgctTAGCTGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGt         <  1:1051084/150‑1 (MQ=255)
                                                                                                                                              tgctgctTAGCAGCGCACTGCTTGGGGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTg        >  2:8836/1‑150 (MQ=255)
                                                                                                                                              tgctgctTAGCAGCGCACTGCGTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTg        >  2:8841/1‑150 (MQ=255)
                                                                                                                                                 tgctTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccg     <  1:716882/150‑1 (MQ=255)
                                                                                                                                                 tgctTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccg     <  1:1425874/150‑1 (MQ=255)
                                                                                                                                                 tgctTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccg     <  2:1666053/150‑1 (MQ=255)
                                                                                                                                                 tgctTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccg     <  1:498158/150‑1 (MQ=255)
                                                                                                                                                  gctTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccgc    <  2:1020962/150‑1 (MQ=255)
                                                                                                                                                    ttAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCa  >  2:1686556/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGCCGGTGGTTACCATGGGCGGGCTGGCGCTGGCCGGAAGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCA  >  NC_000913/360118‑360415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: