Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,691,762 G→A 6.3% P1261L (CCG→CTG)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,7620GA 6.3% 435.3 / 12.5 127P1261L (CCG→CTG) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (73/46);  new base A (4/4);  total (77/50)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 130 total aligned reads displayed.

CTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCA  >  NC_000913/2691614‑2691909
                                                                                                                                                    |                                                                                                                                                   
cTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCgg                                                                                                                                                    >  2:1087473/1‑150 (MQ=255)
 ttAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCggg                                                                                                                                                   >  1:883342/1‑150 (MQ=255)
    aTGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGc                                                                                                                                                >  2:1707421/1‑150 (MQ=255)
    aTGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGc                                                                                                                                                >  2:1707400/1‑150 (MQ=255)
    aTGCCGCATCCGCGATGCAAAACCAATGGGCGGAAGACTTACCACAACTGCTTACGCGCATTGCGGCAAATGCCCATCCATGGGCCAACCACGCCCCAGATTACCGGATGCCAGGAGTTCCTGACAGTACGGAAAACACGTTCCggggcc                                                                                                                                                >  2:1394922/1‑147 (MQ=255)
       ccgccgccGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAGTGCGGAAAATGCGCCTCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGCGGTGCTGACAGTACGGACAACACGTTCCGGGTGCGGc                                                                                                                                             >  2:980265/7‑150 (MQ=255)
       ccGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGc                                                                                                                                             >  2:879653/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGca                                                                                                                                            >  2:165289/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGca                                                                                                                                            >  1:1050631/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGca                                                                                                                                            >  1:1624030/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGca                                                                                                                                            >  1:1050596/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGca                                                                                                                                            >  1:1050644/1‑150 (MQ=255)
        cGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCAGTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTGTCCGGATGCCAGGAGGTGCTGACAGTACGGACAACACGTTCCGGGTGCGGca                                                                                                                                            >  2:924009/1‑150 (MQ=255)
           aTCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGcatca                                                                                                                                         >  2:1035953/1‑150 (MQ=255)
              cGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATaa                                                                                                                                      >  1:572885/1‑150 (MQ=255)
              cGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATaa                                                                                                                                      >  1:38125/1‑150 (MQ=255)
               gggCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAAt                                                                                                                                     >  2:96544/1‑150 (MQ=255)
               gggCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAAt                                                                                                                                     >  2:96450/1‑150 (MQ=255)
                    gCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGa                                                                                                                                <  1:1451542/150‑1 (MQ=255)
                     cAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGAc                                                                                                                               >  1:1198362/1‑150 (MQ=255)
                     cAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGAc                                                                                                                               >  2:1695138/1‑150 (MQ=255)
                       aaaCCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTc                                                                                                                             <  2:425066/150‑1 (MQ=255)
                       aaaCCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTc                                                                                                                             >  1:1632664/1‑150 (MQ=255)
                           cAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGAcc                                                                                                                         >  2:54245/1‑150 (MQ=255)
                           cAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGAcc                                                                                                                         >  1:1223175/1‑150 (MQ=255)
                           cAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGAcc                                                                                                                         >  1:1521685/1‑150 (MQ=255)
                                ggCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACttt                                                                                                                    <  2:1349063/150‑1 (MQ=255)
                                 gCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTc                                                                                                                   >  1:992290/1‑150 (MQ=255)
                                  cTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCAcggtca                                                                                                                  >  2:965951/1‑145 (MQ=255)
                                  cTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCa                                                                                                                  >  2:1123890/1‑150 (MQ=255)
                                   tGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAg                                                                                                                 >  2:394604/1‑150 (MQ=255)
                                   tGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAg                                                                                                                 >  2:211134/1‑150 (MQ=255)
                                       gacTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCAAGTATTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGAGGCATCATAATGGTGACTCGACCACTTTCAGTCGt                                                                                                             >  2:237656/1‑150 (MQ=255)
                                         cTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGa                                                                                                           <  2:742425/150‑1 (MQ=255)
                                              cccAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGcc                                                                                                      >  1:1238429/1‑150 (MQ=255)
                                                cAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGt                                                                                                    >  2:902917/1‑150 (MQ=255)
                                                  aCTGCTTACGCGCAGTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTaa                                                                                                  <  1:227541/150‑1 (MQ=255)
                                                   cTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAAt                                                                                                 <  1:1153796/150‑1 (MQ=255)
                                                    tGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATa                                                                                                >  2:81135/1‑150 (MQ=255)
                                                    tGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATa                                                                                                >  2:1223641/1‑150 (MQ=255)
                                                    tGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATa                                                                                                >  2:81019/1‑150 (MQ=255)
                                                                ttGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGaa                                                                                    <  2:1029622/150‑1 (MQ=255)
                                                                ttGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGaa                                                                                    <  2:43751/150‑1 (MQ=255)
                                                                ttGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGaa                                                                                    <  1:528457/150‑1 (MQ=255)
                                                                 tGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAAc                                                                                   >  2:430250/1‑150 (MQ=255)
                                                                 tGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAAc                                                                                   >  2:430237/1‑150 (MQ=255)
                                                                 tGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAAc                                                                                   >  2:430234/1‑150 (MQ=255)
                                                                     gAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGtt                                                                               >  2:1196916/1‑150 (MQ=255)
                                                                             gcATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAg                                                                       >  2:432573/1‑150 (MQ=255)
                                                                             gcATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAg                                                                       >  1:1420018/1‑150 (MQ=255)
                                                                             gcATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAg                                                                       >  2:1448810/1‑150 (MQ=255)
                                                                               aTCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGcc                                                                     <  2:351764/150‑1 (MQ=255)
                                                                                   aTGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGt                                                                 <  1:926770/150‑1 (MQ=255)
                                                                                   aTGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGt                                                                 >  1:1083106/1‑150 (MQ=255)
                                                                                       gCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGt                                                             <  2:1624030/150‑1 (MQ=255)
                                                                                       gCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGt                                                             <  2:1050644/150‑1 (MQ=255)
                                                                                       gCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCAGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGt                                                             <  2:1050631/150‑1 (MQ=255)
                                                                                        ccATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGtt                                                            >  2:58089/1‑150 (MQ=255)
                                                                                         cATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGttt                                                           >  2:213139/1‑150 (MQ=255)
                                                                                         cATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGttt                                                           >  2:778930/1‑150 (MQ=255)
                                                                                           tCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCa                                                         <  1:204260/150‑1 (MQ=255)
                                                                                             cTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGt                                                       <  1:257169/150‑1 (MQ=255)
                                                                                                 ccccAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACt                                                   >  1:943786/1‑150 (MQ=255)
                                                                                                   ccAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACttt                                                 >  2:305553/1‑150 (MQ=255)
                                                                                                     aGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATTCCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGc                                               <  1:996371/150‑1 (MQ=255)
                                                                                                     aGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGc                                               <  1:587956/150‑1 (MQ=255)
                                                                                                     aGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGc                                               <  1:587940/150‑1 (MQ=255)
                                                                                                       ttttCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCg                                             <  1:780145/150‑1 (MQ=255)
                                                                                                       tggtCCGGATGCCAGGCGGTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAAGGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCg                                             <  2:664931/147‑1 (MQ=255)
                                                                                                         ttCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGaa                                           <  2:992290/150‑1 (MQ=255)
                                                                                                           ccGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGt                                         <  2:757740/150‑1 (MQ=255)
                                                                                                            cGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGtt                                        >  2:1610300/1‑150 (MQ=255)
                                                                                                            cGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGtt                                        >  2:1057906/1‑150 (MQ=255)
                                                                                                              gATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTAt                                      <  1:286182/150‑1 (MQ=255)
                                                                                                              gATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTAt                                      <  1:331928/150‑1 (MQ=255)
                                                                                                              gATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTAt                                      <  1:447453/150‑1 (MQ=255)
                                                                                                               aTGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATc                                     <  2:873161/150‑1 (MQ=255)
                                                                                                                tGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCg                                    <  2:445188/150‑1 (MQ=255)
                                                                                                                 gCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGa                                   <  2:886568/150‑1 (MQ=255)
                                                                                                                 gCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGa                                   <  2:1346204/150‑1 (MQ=255)
                                                                                                                   cAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACa                                 <  2:1545614/150‑1 (MQ=255)
                                                                                                                   cAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACa                                 >  2:1447907/1‑150 (MQ=255)
                                                                                                                   cAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGACGAAGTTATCGACa                                 >  1:91419/1‑150 (MQ=255)
                                                                                                                      gAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGCGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAg                              >  2:908023/1‑150 (MQ=255)
                                                                                                                      gAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAAAGGTGACTCGACCACATTAAGTCGTGACTGCCGTAAAACCGTTAGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTAAGTGACATTTCCGAAGTTATAGACATAg                              >  1:162769/1‑150 (MQ=255)
                                                                                                                           gCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAg                         >  1:1514981/1‑150 (MQ=255)
                                                                                                                             tGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTg                       >  2:761181/1‑150 (MQ=255)
                                                                                                                             tGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTg                       >  2:378313/1‑150 (MQ=255)
                                                                                                                             tGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTg                       >  2:378290/1‑150 (MQ=255)
                                                                                                                                 aGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACACAGCGCAGTGccac                   <  1:472272/150‑1 (MQ=255)
                                                                                                                                 aGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACACAGCGCAGTGccac                   <  2:899290/150‑1 (MQ=255)
                                                                                                                                  gTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTCTCGACATAGCGCAGTGccacc                  >  2:264531/1‑150 (MQ=255)
                                                                                                                                  gTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGccacc                  <  1:1271895/150‑1 (MQ=255)
                                                                                                                                   tACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGccacca                 >  2:981414/1‑150 (MQ=255)
                                                                                                                                   tACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGccacca                 >  2:166369/1‑150 (MQ=255)
                                                                                                                                    aCGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAg                >  1:145351/1‑150 (MQ=255)
                                                                                                                                        aaaaCACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCt            >  1:705180/1‑150 (MQ=255)
                                                                                                                                             acGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCt       <  2:1238429/150‑1 (MQ=255)
                                                                                                                                                ttCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTc    >  1:351410/1‑150 (MQ=255)
                                                                                                                                                ttCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTc    >  2:1440033/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTAATGCCGCATCCGGGCTGCAAAACCAATGGGCTGACGACTTACCCCAACTGCTTACGCGCATTGCGGAAAATGCGCATCCATGGGCCATCCTCGCCCCAGTTTTCCGGATGCCAGGAGTTGCTGACAGTACGGAAAACACGTTCCGGGTGCGGCATCATAATGGTGACTCGACCACTTTCAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCA  >  NC_000913/2691614‑2691909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑