Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	45,367	C→T	23.5%	S396S (AGC→AGT)	fixC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	45,367	0	C	T	23.5%	30.4 / 5.8	17	S396S (AGC→AGT)	fixC	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (4/9);  new base T (2/2);  total (6/11)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACTG  >  NC_000913/45218‑45495                                                                                                                                                      |                                                                                                                                 gAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAgc                                                                                                                                  >  2:170026/1‑150 (MQ=255)      tCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGTGCGcc                                                                                                                             >  2:241145/1‑150 (MQ=255)      tCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGTGCGcc                                                                                                                             >  2:241147/1‑150 (MQ=255)                           tCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAAt                                                                                                        <  1:246990/150‑1 (MQ=255)                               cTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAAtcctc                                                                                                    <  1:66377/150‑1 (MQ=255)                                     gATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCAc                                                                                              >  2:28933/1‑150 (MQ=255)                                                 tACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTg                                                                                  >  2:249742/1‑150 (MQ=255)                                                         acgaCCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTtcat                                                                          <  1:167887/147‑1 (MQ=255)                                                                            aaCCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGc                                                       <  2:32753/150‑1 (MQ=255)                                                                                    cATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAgg                                               >  1:61693/1‑150 (MQ=255)                                                                                                    ccGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGa                               <  2:66587/150‑1 (MQ=255)                                                                                                           tGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTcc                        <  2:151804/150‑1 (MQ=255)                                                                                                                       tgGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGa            <  2:44530/150‑1 (MQ=255)                                                                                                                        ggCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGAc           <  2:202848/150‑1 (MQ=255)                                                                                                                           gcgTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTc        >  2:17656/1‑150 (MQ=255)                                                                                                                                aCCTGTTCACCATTGATGGCAGTGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACt   <  1:241147/150‑1 (MQ=255)                                                                                                                                aCCTGTTCACCATTGATGGCAGTGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACt   <  1:241145/150‑1 (MQ=255)                                                                                                                                 ccTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACTg  <  1:249742/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                 GAATATCGTCAGCATCTTGAGAGTGGTCCGCTGCGCGATATGCGTATGTACCAGAAACTACCGGCGTTCCTTGATAACCCACGCATGTTTAGCGGCTACCCGGAGCTGGCGGTGGGTGTGGCGCGTGACCTGTTCACCATTGATGGCAGCGCGCCGGAACTGATGCGCAAGAAAATCCTCCGCCACGGCAAGAAAGTGGGCTTCATCAATCTAATCAAGGATGGCATGAAAGGAGTGACCGTTTTATGACTTCTCCCGTCAATGTGGACGTCAAACTG  >  NC_000913/45218‑45495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑