Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,311,056 | T→A | 28.6% | H160L (CAC→CTC) | apbE ← | putative thiamine‑synthetic flavin transferase lipoprotein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,311,056 | 0 | T | A | 28.6% | 27.6 / 15.1 | 21 | H160L (CAC→CTC) | apbE | putative thiamine‑synthetic flavin transferase lipoprotein |
Reads supporting (aligned to +/- strand): ref base T (9/6); new base A (3/3); total (12/9) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 |
CGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTCGCGCCAATGCGCAGC > NC_000913/2310918‑2311205 | cGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCg > 2:19967/1‑150 (MQ=255) tGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatc > 2:165904/1‑150 (MQ=255) tGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatc > 2:165902/1‑150 (MQ=255) caacAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTATTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatcgcatc < 2:116617/147‑1 (MQ=255) cATCAAGCGTGCCAAATCATCCGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTcatcgcatc < 2:116614/150‑1 (MQ=255) tCAAGCGTGCCAATTGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGa > 1:196854/1‑150 (MQ=255) aaGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATc > 1:205517/1‑150 (MQ=255) aaGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATc > 1:22512/1‑150 (MQ=255) aaGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATc > 1:102279/1‑150 (MQ=255) aaTGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGc > 1:134255/1‑150 (MQ=255) ggAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGcc > 2:102303/1‑150 (MQ=255) ggAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGcc > 2:74110/1‑150 (MQ=255) ggAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGcc > 2:63738/1‑150 (MQ=255) gaTCGACATATAACTCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGc < 1:140747/150‑1 (MQ=255) tCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGccc < 2:58907/150‑1 (MQ=255) gACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCca < 1:227055/150‑1 (MQ=255) atTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCa < 1:170035/150‑1 (MQ=255) ttgatGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTc < 2:102279/150‑1 (MQ=255) ttgatGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTc < 2:205517/150‑1 (MQ=255) ttgatGACCGTCAGGAGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTc < 2:22512/150‑1 (MQ=255) tCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTCGCGCCAATgc > 2:87505/1‑150 (MQ=255) tGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTCGCGCCAATGCGCAGc > 1:156399/1‑150 (MQ=255) tGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTCGCGCCAATGCGCAGc > 1:156388/1‑150 (MQ=255) tGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCAACGCGCCATCGGTCTTCGCGCCAATGCGCAGc > 1:174534/1‑150 (MQ=255) | CGGGAAATCCCTTCCTGCTCCATCAAGCGTGCCAAATGATCGGCCGCATAGCCTTCGCCGACGGTGGAGAGATCGACATATAAATCCGGCAGGTCTTTTTGCAGATATTGCTGATGCGACTGATTGATGACCGTCAGGTGCTGTAAGCCGGTTTTGGCTTTCATCGCATCGATCTGTTCCTGGCTCGGAATTTGAACCGGCTGTTGTTCCGGGCCAAAGCCCCACAGATTCACCAGCGGCCCGACGGTTATATCCATCGCGCCATCGGTCTTCGCGCCAATGCGCAGC > NC_000913/2310918‑2311205 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |