Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,832,057	G→A	21.0%	A78V (GCG→GTG)	norR ←	anaerobic nitric oxide reductase DNA‑binding transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,832,057	0	G	A	21.0%	38.1 / 5.5	19	A78V (GCG→GTG)	norR	anaerobic nitric oxide reductase DNA‑binding transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base A (2/2);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCAGGTTTTGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGT  >  NC_000913/2831915‑2832202                                                                                                                                               |                                                                                                                                                  gATCAGGTTTTGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAg                                                                                                                                            >  1:137138/1‑150 (MQ=255)  aTCAGGTTTTGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGc                                                                                                                                           >  2:244110/1‑150 (MQ=255)        ttttGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCACTTCCAGCCGTGGa                                                                                                                                     >  1:91824/1‑150 (MQ=255)        ttttGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCACTTCCAGCCGTGGa                                                                                                                                     >  1:91849/1‑150 (MQ=255)           tGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGc                                                                                                                                  <  1:48029/150‑1 (MQ=255)              ccGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCt                                                                                                                               >  2:108410/1‑150 (MQ=255)                                            tGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATcc                                                                                                 <  1:20729/150‑1 (MQ=255)                                                    cAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAg                                                                                         <  2:165247/150‑1 (MQ=255)                                                    cAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAg                                                                                         <  2:235836/150‑1 (MQ=255)                                                                                            ggCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCACGCGGACTAAACTGCCGCGAATCGTaa                                                 >  2:79204/1‑150 (MQ=255)                                                                                                           gCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCCGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGACGCGTCACGTAGCAACGCAGAc                                  >  2:213969/1‑150 (MQ=255)                                                                                                                        ccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa                     <  2:38475/150‑1 (MQ=255)                                                                                                                        ccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa                     <  2:95116/150‑1 (MQ=255)                                                                                                                        ccacATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa                     <  2:95137/150‑1 (MQ=255)                                                                                                                        ccacATCCCCGGCGCGGGCAATCACTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa                     <  2:91824/150‑1 (MQ=255)                                                                                                                        ccacATCCCCGGCGCGGGCAATCACTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCa                     <  2:91849/150‑1 (MQ=255)                                                                                                                         cacaTCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAg                    >  1:211431/1‑150 (MQ=255)                                                                                                                                           aaTCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggt  >  1:62976/1‑150 (MQ=255)                                                                                                                                           aaTCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCgtggt  >  1:49361/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                  GATCAGGTTTTGCCCGGCAAACAATGGCAGACCAACGCAGGCGTGAACCTTCAGACTCTCCTGCCCAGGAATCAAACCGTCATAGGGATCGGGCAATTCGCTGTCTGCGGGAAAGCGCACCACATCCCCGGCGCGGGCAATCGCTTCCAGCCGTGGATGCCCTTCCAGCGCAAAGCGTCTACCGAGTACATCCTTTGCCAGACCGTCGATGGCAAGCGGAATAAACTGCCGCGAATCGTAACGTAGCAACGCAGACGCATCGCACTCCAGCACCTGACGTAGCGTGGT  >  NC_000913/2831915‑2832202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑