Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,958,329	C→T	26.7%	A186T (GCA→ACA)	ptrA ←	protease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,958,329	0	C	T	26.7%	21.5 / 6.0	15	A186T (GCA→ACA)	ptrA	protease III
Reads supporting (aligned to +/- strand):  ref base C (7/4);  new base T (2/2);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCT  >  NC_000913/2958219‑2958459                                                                                                               |                                                                                                                                   gTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACgcgc                                                                                             <  2:227626/150‑1 (MQ=255)            tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAtt                                                                                  >  2:40913/1‑150 (MQ=255)               gcgcCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCCTCCCGTCACGCGTACGCGCCATGGTTAATTCAg                                                                               >  2:6123/1‑150 (MQ=255)               gcgcCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAg                                                                               >  2:71602/1‑150 (MQ=255)                cgcCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGc                                                                              <  2:202685/150‑1 (MQ=255)                          aCCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGTGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCa                                                                    >  2:22564/1‑150 (MQ=255)                          aCCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGTGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCa                                                                    >  2:31361/1‑150 (MQ=255)                                                      tttCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                                        >  1:158323/1‑150 (MQ=255)                                                                accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGATAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAgg                              >  2:158/1‑150 (MQ=255)                                                                 ccaccaGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGt                             <  2:245011/150‑1 (MQ=255)                                                                                    cGGGGTGTGCCGGGTTAATGGTTTCTGTGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCa          <  1:22564/150‑1 (MQ=255)                                                                                    cGGGGTGTGCCGGGTTAATGGTTTCTGTGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCa          <  1:31361/150‑1 (MQ=255)                                                                                      gggTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAgg        >  1:161358/1‑150 (MQ=255)                                                                                            gCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCGTCGGCCAGGCGGTCt  <  2:28654/150‑1 (MQ=255)                                                                                            gCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCt  >  1:94502/1‑150 (MQ=255)                                                                                                               |                                                                                                                                   GTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCATCGGCCAGGCGGTCT  >  NC_000913/2958219‑2958459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑