| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,000,354 | G→A | 26.7% | D4N (GAT→AAT) | xdhA → | xanthine dehydrogenase, molybdenum binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,000,354 | 0 | G | A | 26.7% | 21.9 / 6.3 | 15 | D4N (GAT→AAT) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (8/3); new base A (2/2); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGA > NC_000913/3000213‑3000488 | cccTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATATTTTCCATAAGAAACAAAAGTTAAAAAAGAGGGGATTTCTATTGAAGCGCGGGAAGCAACCGCGACCGGTGAATCATGCATGCGCGTCGGTGCCAtt > 1:156959/1‑150 (MQ=255) tAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTCAGGTCACCGGGCGGGCACGatat > 2:147629/1‑150 (MQ=255) tAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACAGCTACGTGTGAATCATGCATGCGCGTCGATGACATTGCTAAGGTCACCGGGCGGGCACGatat > 2:72998/1‑150 (MQ=255) ccTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGACGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGag > 1:243065/1‑149 (MQ=255) ccATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATgtgt < 2:74231/150‑1 (MQ=255) aTAAGAACCAAATGTTCAAATCGAGGGGATATCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTa > 1:194950/1‑150 (MQ=255) aaCCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGa < 1:2299/150‑1 (MQ=255) aGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGc > 1:168102/1‑150 (MQ=255) aGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGc > 1:97829/1‑150 (MQ=255) gcgGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGt < 2:156959/150‑1 (MQ=255) aaGCAACCGCTACGGGTGAATCATGCATGCGCGTCAATGCCATTGCTAAGGTCACCGGGCGGGCAc > 1:158032/1‑66 (MQ=255) aaGCAACCGCTACGGGTGAATCATGCATGCGCGTCAATGCCATTGCTAAGGTCACCGGGCGGGCAc > 1:249916/1‑66 (MQ=255) aaGCAACCGCTACGGGTGAATCATGCATGCGCGTCAATGCCATTGCTAAGGTCACCGGGCGGGCAc < 2:158032/66‑1 (MQ=255) aaGCAACCGCTACGGGTGAATCATGCATGCGCGTCAATGCCATTGCTAAGGTCACCGGGCGGGCAc < 2:249916/66‑1 (MQ=255) tCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGa > 1:209808/1‑150 (MQ=255) | CCCTAACGCTTATCGTCGTTTCTGGCGTAAATCTTGCCTGCTTAGACTAAATCTTTGCCATAAGAACCAAATGTTCAAATCGAGGGGATTTCTATGGAAGCGCGGGAAGCAACCGCTACGGGTGAATCATGCATGCGCGTCGATGCCATTGCTAAGGTCACCGGGCGGGCACGATATACTGACGATTATGTTATGGCGGGCATGTGTTATGCGAAATATGTACGTAGCCCTATCGCACATGGTTATGCCGTAAGTATTAATGATGAACAAGCCAGA > NC_000913/3000213‑3000488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |