| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,591,925 | C→T | 17.4% | G134D (GGC→GAC) | ugpB ← | sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,591,925 | 0 | C | T | 17.4% | 53.2 / 5.5 | 23 | G134D (GGC→GAC) | ugpB | sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (5/14); new base T (2/2); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
TGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAAC > NC_000913/3591776‑3592067 | tGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTATTCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGc < 1:69661/150‑1 (MQ=255) gAGGCTTTCAGTTTAGCGGCATAGTCCGCCACATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATATAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGtt > 1:117199/1‑150 (MQ=255) ggCTTTGAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGtttt < 1:175595/150‑1 (MQ=255) ggCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGtttt < 1:242086/150‑1 (MQ=255) tttCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCt < 2:165193/150‑1 (MQ=255) tttCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCt < 2:131861/150‑1 (MQ=255) tCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGagt > 2:120734/1‑150 (MQ=255) cAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGt < 1:40829/150‑1 (MQ=255) gATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCg < 1:57915/150‑1 (MQ=255) gATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCg < 2:77822/150‑1 (MQ=255) gATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCg < 2:77820/150‑1 (MQ=255) aTCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCgg < 2:24608/150‑1 (MQ=255) tttCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGa < 2:47717/150‑1 (MQ=255) ctgcTGTACCTGGTCTAATCCTTCTTTCTTGAAGGCGTCTTTGTTGTAATTGAGTACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTATCCTGAAACCGTCGGCACAAACTTCGACTCATc < 2:208376/148‑1 (MQ=255) cggcTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATc < 2:208377/150‑1 (MQ=255) cTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGaa < 1:8355/150‑1 (MQ=255) tttCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTaaa > 1:202484/1‑150 (MQ=255) tttCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTaaa > 1:202481/1‑150 (MQ=255) gAATGGCTGGGAGAGTAAGTGGTCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGt < 2:86952/107‑1 (MQ=255) gAATGGCTGGGAGAGTAAGTGGTCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGt < 2:86978/107‑1 (MQ=255) gAATGGCTGGGAGAGTAAGTGGTCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGt > 1:86978/1‑107 (MQ=255) gAATGGCTGGGAGAGTAAGTGGTCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGt > 1:86952/1‑107 (MQ=255) gagaGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCa < 1:79162/150‑1 (MQ=255) tAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCAGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAAc > 1:228855/1‑150 (MQ=255) | TGCCGGAGGCTTTCAGTTTCGCGGCATAGTCCGCCAGATCCTGCCAGGTTTTCGGCGGCTGTTCCGGGTCTAATCCTGCTTTCTTGAAGGCGTCTTTGTTGTAATAGAGAACGGGGGTCGAGCTGTTGAATGGCTGGGAGAGTAAGTGGCCCGTTTTGCTGTCGGAGTAGTAACCTGAAACCGTCGGCACAAACTGCGACTCATCGAACTGAATCCCTGCCTCTTTAAACACGTCATACACCGGTTTAATGGCTTTCGACGCCATCATGGTGGCGGTGCCAACTTCATAAAC > NC_000913/3591776‑3592067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |