| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,150,140 | G→A | 17.4% | G42G (GGC→GGT) | eptC ← | LPS heptose I phosphoethanolamine transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,150,140 | 0 | G | A | 17.4% | 52.3 / 5.5 | 23 | G42G (GGC→GGT) | eptC | LPS heptose I phosphoethanolamine transferase |
| Reads supporting (aligned to +/- strand): ref base G (4/15); new base A (2/2); total (6/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
AGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGAATGCATAGTA > NC_000913/4149992‑4150269 | aGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGTCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGc < 1:185707/150‑1 (MQ=255) aTGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTACCa > 2:62237/1‑150 (MQ=255) aTGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATAACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTACCa > 2:245383/1‑150 (MQ=255) tagCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCa < 2:228128/150‑1 (MQ=255) tagCACAGCGCCGCCAGAGAGGCCGCCCATAGAACCACGACGATTACTGCGGCAATAATTTTAATCCTCTTCGGAAAGAGGAATACCGGGATCAACCACAGCTAACTGAATTACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCa < 2:103525/150‑1 (MQ=255) ccAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAaataa > 2:200348/1‑150 (MQ=255) ccAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAaataa > 2:228117/1‑150 (MQ=255) agagGCCGCACATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGATAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGc < 1:196321/150‑1 (MQ=255) cATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCaga < 1:77016/150‑1 (MQ=255) ccGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAAc < 1:166855/150‑1 (MQ=255) ccGATTACTGCGGCAATAATTTTAATCCGCGTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAAc < 1:100871/150‑1 (MQ=255) aaTCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTAAAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAAcc > 2:50980/1‑150 (MQ=255) aCCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAaga < 1:119247/150‑1 (MQ=255) aaCCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGcc > 2:95158/1‑150 (MQ=255) aCTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCtgt < 2:22453/150‑1 (MQ=255) aCTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCtgt < 2:22450/150‑1 (MQ=255) aCTGAATAACAGCGAGTCGCGAATGCCGTTAGTACCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCtgt < 1:62237/150‑1 (MQ=255) aCTGAATAACAGCGAGTCGCGAATGCCGTTAGTACCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCtgt < 1:245383/150‑1 (MQ=255) cTGAATAACCGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCtgtg < 2:39115/150‑1 (MQ=255) aaTAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGaa < 1:187416/150‑1 (MQ=255) gAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGAATGCATAGTa < 2:159008/150‑1 (MQ=255) gAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGAATGCATAGTa < 2:159007/150‑1 (MQ=255) gAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGAATGCATAGTa < 2:33052/150‑1 (MQ=255) | AGATGACGTAGTAGCACAGCGCCGCCAGAGAGGCCGCCCATAGCACCACGCCGATTACTGCGGCAATAATTTTAATCCGCTTCGGAAAGAGGAATACCGGGATCAACCACAGCGAACTGAATAACAGCGAGTCGCGAATGCCGTTAGTGCCACTATAACCACTGATGTAAATAATGGCCTGTAGCAGAGTAGAGAAAAACCAAAAGTAGAGCAGTGCCCAACCCAGGGCTTTCCAGCTAAAAAGAGGTTTAGCCTGGACTTCTGTGGAATGCATAGTA > NC_000913/4149992‑4150269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |