| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,344,839 | C→A | 25.0% | intergenic (+49/+90) | melB → / ← yjdF | melibiose:sodium symporter/DUF2238 family inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,344,839 | 0 | C | A | 25.0% | 26.6 / 7.8 | 16 | intergenic (+49/+90) | melB/yjdF | melibiose:sodium symporter/DUF2238 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (4/8); new base A (2/2); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
ATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGG > NC_000913/4344714‑4344962 | aTAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGa < 2:219709/150‑1 (MQ=255)aTAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGa < 2:235156/150‑1 (MQ=255) cGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGAc > 2:224307/1‑150 (MQ=255) cATGCTGATATTCCGGTCGGTGCCGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGACTTc > 1:185277/1‑150 (MQ=255) tGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGACACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCAcgcc > 2:183718/1‑150 (MQ=255) tGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGACACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCAcgcc > 2:183750/1‑150 (MQ=255) gAAAGCCTGACGTGGACACTAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCCTCAACACCGCGCAAggggtt > 2:235155/1‑145 (MQ=255) gTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGACACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATc < 1:183718/150‑1 (MQ=255) gTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGACACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATc < 1:183750/150‑1 (MQ=255) tGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCa < 2:104881/150‑1 (MQ=255) gACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAg < 1:141548/150‑1 (MQ=255) gACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAg < 1:15381/150‑1 (MQ=255) gACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAg < 1:15387/150‑1 (MQ=255) gACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAg < 1:229678/150‑1 (MQ=255) cGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGt > 2:105851/1‑150 (MQ=255) tGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTgg < 2:64450/150‑1 (MQ=255) | ATAAATATCGCAAAGTACCGCCCGAGCCTGTTCATGCTGATATTCCGGTCGGTGCAGTGAGTGATGTGAAAGCCTGACGTGGACAATAGCTAACGAAAATGGCCTGATGCGATATGTTTATCAGGCCACGTCTTAATGTAATGCTTTGGATTTGCAAATTTTGTAGACCGGATAAGGAATTCACGCCGCATCCGGCATCAACAAAGCGCAAGTTGTTATCCGGTTATCAAGCCAAAGCGCCGTAGCTGG > NC_000913/4344714‑4344962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |