| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,451,483 | G→A | 17.4% | P142P (CCG→CCA) | ytfR → | putative sugar ABC transporter ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,451,483 | 0 | G | A | 17.4% | 55.3 / 5.4 | 23 | P142P (CCG→CCA) | ytfR | putative sugar ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base G (7/12); new base A (2/2); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTACTGTTTGACCTGATGCGTC > NC_000913/4451365‑4451625 | aTAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCaaa < 1:97438/150‑1 (MQ=255)aTAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCaaa < 1:97432/150‑1 (MQ=255) tCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAAtcgt > 2:170160/1‑150 (MQ=255) cgAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGc < 1:168706/150‑1 (MQ=255) aaCGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCACTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGAtc > 1:179210/1‑150 (MQ=255) aaCGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCACTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGAtc > 1:179211/1‑150 (MQ=255) ccTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCaa > 1:155526/1‑150 (MQ=255) ccTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCaa > 1:88292/1‑150 (MQ=255) cTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCaaa < 1:116583/150‑1 (MQ=255) cTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCaaa < 1:240856/150‑1 (MQ=255) cAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCt < 1:115616/150‑1 (MQ=255) agagATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGa > 1:29769/1‑150 (MQ=255) aTGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGaa < 2:232717/150‑1 (MQ=255) aGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCg < 1:11955/150‑1 (MQ=255) gcCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGt < 1:217844/150‑1 (MQ=255) aTCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGACGt > 1:232013/1‑150 (MQ=255) aTCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGt > 1:232019/1‑150 (MQ=255) ttACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGa > 1:223866/1‑150 (MQ=255) aCGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGt < 1:109002/150‑1 (MQ=255) ggTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTa < 1:113927/150‑1 (MQ=255) ccTCGACGTGCGCGAACCACTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTACTGTTTGa < 2:179211/150‑1 (MQ=255) ccTCGACGTGCGCGAACCACTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTACTGTTTGa < 2:179210/150‑1 (MQ=255) gcgAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTACTGTTTGACCTGATGCGTc < 2:33852/150‑1 (MQ=255) | ATAATCTATTTATAGGCCGCGAACCCAAACGCTTCGGCCTTCTACGCCGCAAAGAGATGGAAAAGCGCGCCACCGAACTGATGGCATCTTACGGTTTCTCCCTCGACGTGCGCGAACCGCTCAACCGCTTTTCAGTCGCGATGCAGCAAATCGTCGCTATTTGTCGGGCTATCGATCTCTCTGCCAAAGTGCTGATCCTCGATGAACCCACCGCCAGTCTCGACACCCAGGAAGTGGAGTTACTGTTTGACCTGATGCGTC > NC_000913/4451365‑4451625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |