| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 664,812 | C→T | 18.1% | G127R (GGG→AGG) | rlpA ← | septal ring protein, suppressor of prc, minor lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 664,812 | 0 | C | T | 18.1% | 50.4 / 4.1 | 22 | G127R (GGG→AGG) | rlpA | septal ring protein, suppressor of prc, minor lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (10/8); new base T (2/2); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCC > NC_000913/664680‑664951 | caaTAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGa < 2:58204/150‑1 (MQ=255) aCGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTggg > 2:107922/1‑150 (MQ=255) ttGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGa > 2:115428/1‑150 (MQ=255) ttgttTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGtt < 2:194126/150‑1 (MQ=255) tttGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGa > 1:193751/1‑150 (MQ=255) tttGACGTGTTAAGACGGTCAGCTGCCGAGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATACTGGCGTAGCTGGGGATCGGACGCGTTGGa > 2:188256/1‑150 (MQ=255) gtgtTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCt > 1:190588/1‑150 (MQ=255) gtgtTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCt > 1:80437/1‑150 (MQ=255) gtgtTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTAGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGACGCGTTGGATGGGCt > 1:190579/1‑150 (MQ=255) tgtTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTg < 2:248338/150‑1 (MQ=255) tCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCTGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCtgt > 2:51472/1‑150 (MQ=255) tCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCTGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCtgt > 2:147704/1‑150 (MQ=255) aaTAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCaga < 1:146837/150‑1 (MQ=255) aaTAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCaga < 1:146840/150‑1 (MQ=255) tAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAgg < 1:135229/150‑1 (MQ=255) tAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAgg < 1:135221/150‑1 (MQ=255) ccGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCg > 1:222049/1‑150 (MQ=255) cgATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGca < 2:222049/150‑1 (MQ=255) aTTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATa > 1:48576/1‑150 (MQ=255) cacGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAg > 2:67128/1‑150 (MQ=255) aTCATTCGCCTGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTccc < 1:51472/150‑1 (MQ=255) aTCATTCGCCTGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTccc < 1:147704/150‑1 (MQ=255) | CAATAATCGGATCGATACGAACTTTGGTGTTGTTTGACGTGTTAAGACGGTCAGCTGCCGCGCGAGAAAGTGAAATAACGCGGTCGTTGCCGTAAGGGCCGCGATCATTAATGCGCACCACGATCATTCGCCCGTTAGCCAGGTTAGTGATTCTGGCGTAGCTGGGGATCGGAAGCGTTGGATGGGCTGCCGTCAGCTGTGTCGGATCGAATGCTTCGCCAGAGGCCGTCAGGTTACTGCCGGGTTCGGCATCATAGATTGCCGCCAGTCCC > NC_000913/664680‑664951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |