Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 10.4% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA10.4% 312.9 / 24.9 130noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (59/53);  new base A (7/6);  total (69/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 130 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACA  >  NC_000913/1287001‑1287295
                                                                                                                                                    |                                                                                                                                                   
ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    <  2:537029/150‑1 (MQ=255)
 tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAg                                                                                                                                                   <  1:3653215/150‑1 (MQ=255)
  ccccGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                                  <  2:2316857/150‑1 (MQ=255)
   cccGCAGGGGCAGAATTTTTGCTTGCGGATTCTCTTGATAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                 <  1:1826103/150‑1 (MQ=14)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                                >  1:1617605/1‑150 (MQ=255)
      gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTATCGCGGGCGCACGacgcgga                                                                                                                                              >  2:3691926/1‑144 (MQ=14)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGt                                                                                                                                       >  1:3195542/1‑150 (MQ=255)
                 atatTTGAGTGCGGATTCGCGTGAGAGTTCCGGGCCTTTTGACCGTGAGGGGGGTGGGGGCAGGATTACTCCGCGCGGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                   <  2:3820431/150‑1 (MQ=14)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                                >  2:107811/1‑150 (MQ=255)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTCGTCGAAGCt                                                                                                                                >  2:2457450/1‑150 (MQ=255)
                     ttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                               >  1:2382922/1‑150 (MQ=255)
                        aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                            >  2:124294/1‑150 (MQ=255)
                           gCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTTTCGCTCGAATAGAAACTTTGTCGAAGGTTATCaaa                                                                                                                         >  2:1937117/1‑148 (MQ=11)
                             ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCt                                                                                                                       >  2:89891/1‑150 (MQ=255)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAATGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCTCTCAAGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCATTccccc                                                                                                                 >  2:3962206/1‑149 (MQ=14)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCGAGCAGGCGCTCCAGTCGAAACTAGGTCGAAGCGTCTCAAACATccccc                                                                                                                 >  2:426223/1‑149 (MQ=14)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCTCGCGGGCGCACGAGTCGAAACTTGGTAGAAGCTTCTCATCCTTCCCCGCa                                                                                                               >  1:556929/1‑150 (MQ=14)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGACGCTTCTCATCCTTCCCCGCag                                                                                                              >  2:2611459/1‑149 (MQ=255)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                              <  1:2082937/150‑1 (MQ=255)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCACGCTGGCGCTCGAGTCGAACCATGGTCGAAGCGTCTCAACCATccccccct                                                                                                              >  1:3453146/1‑146 (MQ=14)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTAAACCTTACCCCCaa                                                                                                              >  1:4017062/1‑149 (MQ=14)
                                       gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTGGCGCTCGAGTCGATCCGTGGTCGAACCGACTCAACCTTCCCCGCaag                                                                                                             >  2:3636857/1‑148 (MQ=14)
                                        agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCGCACAAGCTGGCGCGCGAGACGAACCAAGGTCGAAGCATCTAAACCATACCCGCAAgg                                                                                                            >  1:3473459/1‑150 (MQ=14)
                                         gagTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                           <  2:3543181/150‑1 (MQ=255)
                                              cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGACTCGAACCTTTGTCGAAGCTTCTCAACCATCCCCGCAAGGGCCGaa                                                                                                      >  2:1570939/1‑150 (MQ=11)
                                               aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACACTCGCAGGCGCGCGAGGCGAAACATGGTCGTAGCGTCTCAACCATACCCCCAAGGGCAGAat                                                                                                     >  1:1215344/1‑150 (MQ=11)
                                                gggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCTCACTCGCTAGCCCGCGAAGGGCAACTTGGTAGTATCATCTAATACTTGCCCGCATGGGCCGaaaa                                                                                                    >  1:1054482/1‑148 (MQ=2)
                                                   aCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                 <  1:3323292/150‑1 (MQ=38)
                                                   aCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                 <  1:3323194/150‑1 (MQ=38)
                                                    cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    >  2:1645568/1‑98 (MQ=33)
                                                    cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    <  1:1645568/98‑1 (MQ=33)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCAACAGTAGAACCTTGGTCGAAACATCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                               >  2:570343/1‑150 (MQ=11)
                                                       ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt                                                                                             <  2:311251/150‑1 (MQ=39)
                                                              tGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCACGCGGGCGCTCGAGTAGGACCAAGGTCGAAGCATCACATACATCCCCCCAGGGGCCGAATATTTAAATTCGGGtt                                                                                      >  1:2471985/1‑150 (MQ=11)
                                                                  ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCAGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCggt                                                                                 >  2:4181085/1‑148 (MQ=255)
                                                                   gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTTCGGAGACGTTg                                                                                >  2:1806481/1‑150 (MQ=255)
                                                                   ggggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                <  1:2433230/148‑1 (MQ=255)
                                                                     ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                              >  2:1330261/1‑150 (MQ=39)
                                                                       tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                         <  1:2745201/106‑1 (MQ=32)
                                                                       tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                         <  1:518458/106‑1 (MQ=32)
                                                                       tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                         >  2:518452/1‑106 (MQ=32)
                                                                       tggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTCGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc                                                                                                                         >  2:2745201/1‑106 (MQ=17)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGGTTGGGagt                                                                           >  2:3395126/1‑148 (MQ=11)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                 <  1:1826104/80‑1 (MQ=34)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                                 >  2:1826104/1‑80 (MQ=34)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                       <  1:1134489/150‑1 (MQ=255)
                                                                             aaGGATTACACAGCGCTCCGCGCTTCGCCCTTCGCGTCGTAGCCTGCGGCAACGCTCTCTCGCTTGCGCTCTAGTCGAACCTTCGTAGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                      <  1:1363367/150‑1 (MQ=14)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                    <  1:3675920/70‑1 (MQ=33)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGGTGGGGAGTTCAGGGAc                                                                  >  2:1134489/1‑150 (MQ=11)
                                                                                    aCTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                               <  1:1262537/150‑1 (MQ=255)
                                                                                    aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                               <  1:685053/150‑1 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                              <  1:2313701/84‑1 (MQ=33)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtc                                                                                                                              >  2:2313701/1‑84 (MQ=33)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTAATTTCGGATTGGTTGGGGAGTTCAGGGACTTTTGaa                                                           >  2:2242890/1‑150 (MQ=255)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:3926087/150‑1 (MQ=255)
                                                                                         gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:1718432/150‑1 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                         <  2:1215344/150‑1 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                         <  1:1451778/150‑1 (MQ=255)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                      <  2:3071416/150‑1 (MQ=255)
                                                                                                gcgcGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtgg                                                   <  1:2409534/150‑1 (MQ=255)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTGGGAAGTTCAGGGTCTTTTGAAAGTGAtggtggt                                               >  2:3213619/1‑150 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGTtggtggggg                                             >  2:534022/1‑150 (MQ=255)
                                                                                                         ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGGAGATTTTATTGCGGATTCGTTGGGTAGGTCCGGGACTGTTGAAAGTGGTGGTGGTggggg                                          >  2:786539/1‑150 (MQ=14)
                                                                                                           gtcgggtcgggGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGATGCTTGGTCGAAGCTTCTCATCCTTCCCCGGATGGGCAGAATATTTTATTGCGGATTCGTTGGGCAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGaa                                        <  1:728429/149‑1 (MQ=14)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           <  1:786679/109‑1 (MQ=255)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           >  2:786679/1‑109 (MQ=255)
                                                                                                                 gcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATAGCGGATTCGTGGGGAAGTTCAGGGACTTTTGCAAGTGTTGGTGGTGGGGGGAGGATTc                                  >  1:468540/2‑150 (MQ=17)
                                                                                                                  cGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCg                                 >  1:3105783/1‑150 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                      <  1:2691144/112‑1 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                      >  2:2691144/1‑112 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                               <  1:3538743/150‑1 (MQ=255)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                              <  1:1330261/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCTGGGTCGAAGCTTCTGATCCTTCCCCGCCGGGGCAGAATAGGTAATTGCGGAGTCGTGGGGAAGTGCAGGGCCGTTTGAACGGGAGGGGGGTGGGGGAAGGATTCGACCCtgc                          >  2:66703/1‑148 (MQ=17)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                          <  2:3305208/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAATTGCGGATTCGTTGGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                          >  1:2600337/1‑150 (MQ=255)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:1890668/116‑1 (MQ=255)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          >  2:1890668/1‑116 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGTACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                       <  1:426223/150‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                       <  1:3962206/150‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAAAATTTAATTGCGGATTAGTTGGGAAGTTAAGGGACTTTTGGAAGTGATGGTGGTGGGGGGAGGATTCGGACCTTAGaa                       >  1:2798744/1‑150 (MQ=17)
                                                                                                                                aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                   >  2:506779/1‑150 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                 >  2:472524/1‑150 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           >  2:662722/1‑92 (MQ=16)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           <  1:662722/92‑1 (MQ=16)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           >  2:662728/1‑92 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           <  1:3004678/92‑1 (MQ=255)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                           >  2:3004678/1‑92 (MQ=255)
                                                                                                                                  gCTCTCTCACTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                 >  2:3125005/1‑150 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          >  2:2191633/1‑107 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:2191633/107‑1 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:2191757/107‑1 (MQ=255)
                                                                                                                                    tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          >  2:2191757/1‑107 (MQ=255)
                                                                                                                                      tctcGCTGCCGCTCGAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg             <  2:495906/150‑1 (MQ=255)
                                                                                                                                         cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa          <  2:3473459/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTTGGCACTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGTAGGATTCGAAACATCGAAGTCGATGAAGGCAGa        >  1:1178023/1‑150 (MQ=17)
                                                                                                                                            tGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGCCTCTGTAATTGCGGATTCGGGGGGAAGGTCCCGGACTGGTGCACGGGATGGTGGTGGGGGAAGGAGTCGCCCCTTCGAAGGCGATGACGGCAGAt       >  2:3498885/1‑150 (MQ=16)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATATTTAATTGCGGTTTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc   <  1:4101242/150‑1 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc   <  1:247896/150‑1 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTCGTCGAAGCTTCTCAACCTTCCCCGCCTGGGCAGAATCTTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc   <  1:3826488/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa  <  2:4180216/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACA  >  NC_000913/1287001‑1287295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: