Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,768 T→C 6.7% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7680TC 6.7% 411.3 / 10.2 132intergenic (+34/+14)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (80/42);  new base C (4/5);  total (84/48)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

GATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG  >  NC_000913/1864621‑1864916
                                                                                                                                                    |                                                                                                                                                    
gatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATcc                                                                                                                                                    <  2:1783154/150‑1 (MQ=255)
gatgGATACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCATAAACGCAAAAAGTGACCAAAGAGAAACCTTCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATcc                                                                                                                                                    <  1:408836/150‑1 (MQ=255)
 atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGTGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                                   <  2:284348/150‑1 (MQ=255)
 atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                                   >  2:2172779/1‑150 (MQ=255)
 atgGCTACAAAACATTTGTGTGTGTAGAAACGGCTTACGCTGCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCACTGCATCCg                                                                                                                                                   >  2:3492237/1‑150 (MQ=255)
  tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                                  <  2:21352/150‑1 (MQ=255)
    gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                                <  2:3185287/150‑1 (MQ=255)
    gCTACAAAACAGTTGTTGGTGTAGAAACGGCTGACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                                <  2:3185325/150‑1 (MQ=255)
     cTACACAACCTGTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcac                                                                                                                                               <  2:11652/150‑1 (MQ=255)
      tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAGAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                              >  2:399550/1‑150 (MQ=255)
      tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCCTTCGCGTTGCGAAACGTTAAGTTACGTTAATGTTGTGGGCCGGGTGCAATGCCTCCGGcccc                                                                                                                                              >  2:3755291/1‑147 (MQ=255)
      tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTCCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                              >  2:767066/1‑150 (MQ=255)
              aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCGGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                                      <  2:398700/150‑1 (MQ=255)
               tttgtttgTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                                                                                     >  2:149199/1‑150 (MQ=255)
               tttgtttgTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                                                                                     >  2:148718/1‑150 (MQ=255)
                  gtttgaGTACAAACGGCTTACGCTTCACAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCAGGCGCAAACCATTCGCGTTGCGAAACGTTAATTTACCTTAAAGATGTGTGCCGGGTGCAAAGCATCCGCCCCACAACCTCACAcc                                                                                                                                  >  2:1302872/1‑150 (MQ=255)
                   tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAccc                                                                                                                                 >  2:2126842/1‑149 (MQ=255)
                   tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                                 >  1:2437968/1‑150 (MQ=255)
                       tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATc                                                                                                                             >  2:432460/1‑150 (MQ=255)
                         tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                           <  2:2287007/150‑1 (MQ=255)
                         tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCa                                                                                                                           >  1:1805037/1‑150 (MQ=255)
                           gAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgc                                                                                                                         >  1:3848717/1‑150 (MQ=255)
                              aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                                      >  1:2440069/1‑150 (MQ=255)
                                   ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGGCACACAACATCACACCAAATCCAGCGCAGtttt                                                                                                                 >  2:2134684/1‑150 (MQ=255)
                                         ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCCGTTTTTCCttt                                                                                                           >  1:1166667/1‑150 (MQ=255)
                                          tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtttt                                                                                                          >  2:144559/1‑150 (MQ=255)
                                          tCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCCGCCCAGTTTTTACtttt                                                                                                          >  1:1106828/1‑150 (MQ=255)
                                             gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGt                                                                                                       >  1:2714328/1‑150 (MQ=255)
                                               aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATGCGGCACACAACATCACACCATATGCAGCGCAGTTTTTGCTTTTGGTGc                                                                                                     >  1:2259969/1‑150 (MQ=255)
                                                aCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGcc                                                                                                    <  2:1018194/150‑1 (MQ=255)
                                                 cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                   >  2:3726382/1‑150 (MQ=255)
                                                 cGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCg                                                                                                   >  2:4136151/1‑150 (MQ=255)
                                                    aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                                >  2:1796208/1‑150 (MQ=255)
                                                    aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                                >  2:3219114/1‑150 (MQ=255)
                                                    aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGTTGGTGCCGGat                                                                                                >  2:2424269/1‑150 (MQ=255)
                                                            aCCAACGAGAACCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTa                                                                                        <  2:3692697/150‑1 (MQ=255)
                                                               aaaGACAACCCTGCACTTCGGGCGCTATCGTTGGGGGTTGCTAACGTTTATTTTCGTTTTTTGTGGTGTGCGGGGTGAACGGCACCCGCCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                                     <  1:765249/150‑1 (MQ=255)
                                                                aaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                    >  2:272109/1‑150 (MQ=255)
                                                                aaGAGAAACCTGCACATCGGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACTTTAATGTTGTGTGCCGGGTGCAATGCATCCGCCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                                    <  1:2819446/150‑1 (MQ=255)
                                                                   agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                                 >  1:3460725/1‑150 (MQ=255)
                                                                        ccTTCACCTCTGGCGCAATCCATTCGCGTTGGGAAACTTTAATTTTCGTTATTTTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                            <  2:6328/150‑1 (MQ=255)
                                                                        ccTGCACATCTGGCGCTATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGGAATGGATGCGGCACACAACATCACACCATTTCCAGCGCAGTTTTTGCTTTTGGTGGCGGTTATGCCTTATCCAGCATAGCt                                                                            >  2:3017049/1‑150 (MQ=255)
                                                                        ccTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCt                                                                            <  1:3128041/150‑1 (MQ=255)
                                                                         cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGGGCCATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTa                                                                           >  2:3306757/1‑150 (MQ=255)
                                                                          tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTaa                                                                          <  2:3535889/150‑1 (MQ=255)
                                                                          tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTaa                                                                          >  2:2691848/1‑150 (MQ=255)
                                                                          tGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGACTTATACAGCATAGCTaa                                                                          >  1:3410086/1‑150 (MQ=255)
                                                                           gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                         >  2:3983064/1‑150 (MQ=255)
                                                                           gCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAt                                                                         >  2:2561909/1‑150 (MQ=255)
                                                                            cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCCCACAACAACACACCCTATCCCGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCGTATCCAGCCAAGCTAAtt                                                                        >  1:2082171/1‑150 (MQ=255)
                                                                            cacaTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGGGCCGGATATGCCTTATCCAGCCTAGCTAAtt                                                                        >  2:1350558/1‑150 (MQ=255)
                                                                             acaTCTGGCGAAATCCATTCGCGTTGCGAAACTTTAATTTACGTTATTGTTGTGTGCCGGGTGAAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTc                                                                       <  1:1218324/150‑1 (MQ=255)
                                                                              caTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTACTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCGTAGCTAATTcc                                                                      <  2:3716667/150‑1 (MQ=255)
                                                                              caTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCGTAGCTAATTcc                                                                      <  2:3717207/150‑1 (MQ=255)
                                                                               aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACTCAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                                     >  2:296180/1‑150 (MQ=255)
                                                                               aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                                     >  2:296257/1‑150 (MQ=255)
                                                                                tCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                                    <  2:3793918/150‑1 (MQ=255)
                                                                                 cTGGCGAAATCCTTTCGGGTTGCGAAACTTTATTTTACTTTATTTTTGTGGGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                                   <  1:1849856/150‑1 (MQ=255)
                                                                                    gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGATGTGTTCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                                >  2:2834469/1‑150 (MQ=255)
                                                                                        aaTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                            >  2:3423686/1‑150 (MQ=255)
                                                                                        aaTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGGGCAATGCATACGGCCCACAACAACACACCAAATCCAGCGCACGTTTTGCATTTGGTGGCGGGTATGGCGTATACAGCCGAGCTAATTCCGCTgtagaac                                                            >  1:4232909/1‑149 (MQ=255)
                                                                                         aTCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGATGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAg                                                           >  2:3566241/1‑150 (MQ=255)
                                                                                           ccATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTGATTCCGCTGAAGAAAGtt                                                         >  2:1773020/1‑150 (MQ=255)
                                                                                             aTTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCCGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGGTTTTCCTTTTGGTGCCGGATATGCCGTATCCACACTATCTAAATCCGCTGAAGAAAGTTCg                                                       >  2:1185134/1‑150 (MQ=255)
                                                                                              ttCGCGTTGCTAAACTTTAATTTACTTTATTGTTGTGTGCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                                      <  1:399550/150‑1 (MQ=255)
                                                                                                      gCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGc                                              <  1:358139/150‑1 (MQ=255)
                                                                                                       cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                             <  1:1026579/150‑1 (MQ=255)
                                                                                                       cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                             >  2:201069/1‑150 (MQ=255)
                                                                                                       cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACCAGCGCAGTTTTTACTTTTGGTGCCGGAGATGCCTTATCCAGCATAGCTAATTCCGCGGAAGAAAGTTCGACCTCAAGca                                             >  2:3660967/1‑150 (MQ=255)
                                                                                                       cGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGCTTTTGGTGCCGGATATGCCTTATCCAGCAAAGCTAATTCCGCTGAAGACAGTTCGACCTCAAGca                                             >  2:3660966/1‑150 (MQ=255)
                                                                                                             gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCg                                       >  2:1237947/1‑150 (MQ=255)
                                                                                                              ttAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGc                                      <  2:651872/150‑1 (MQ=255)
                                                                                                                 aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACCACATCACACCATATCCAGCGCAGTTTTTCCGTGTGGTGCCGGCTATGCCTGATCCCGCATAGCGAAGTCCGCTGGCGCGCGTTCGGCCGCAGGCCCAGCCggctg                                   >  2:2946467/1‑146 (MQ=255)
                                                                                                                 aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                                   <  1:4204835/150‑1 (MQ=255)
                                                                                                                 aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                                   >  2:797474/1‑150 (MQ=255)
                                                                                                                 aTTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                                   <  1:4204891/150‑1 (MQ=255)
                                                                                                                  tttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGGTATGCCTTATCCAGCATAGCTAATTCCGCTGAAGACAGTTCGACCTCAAGCACAGCCGCAttt                                  >  2:3534568/1‑150 (MQ=255)
                                                                                                                       gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgttg                             >  2:1298274/1‑150 (MQ=255)
                                                                                                                       gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATTCCGTATCCAGCATAGCTAATTCCGCTGAAGTAAGTTCGACCTCAAGCACAGCCGCGTTttgttg                             >  2:2276628/1‑150 (MQ=255)
                                                                                                                       gTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTGGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGGTCGACCTCAAGCACAGCCGCATTttggtg                             >  2:661645/1‑150 (MQ=255)
                                                                                                                          tattgttgtGTGCCGGGTGCATTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                           <  2:3984255/147‑1 (MQ=255)
                                                                                                                          tAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                           <  1:1056666/150‑1 (MQ=255)
                                                                                                                          tAATGTTGTGGGCCGGGTGCAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                           <  1:1452149/150‑1 (MQ=255)
                                                                                                                            aTGTTGTGTGGCGGATGGATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTGGTTTTGGTGGCGGTTATGGCTTTTGCAGGATAGGTAATTCCGGTGTAGTAAGTTCGTCCTGAAGGACAGCCGCATTTTGTTggtga                         >  2:695245/1‑147 (MQ=255)
                                                                                                                                  tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTACGTTTGGTGCCCGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa                   >  1:2962755/1‑150 (MQ=255)
                                                                                                                                    tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt                 >  1:610697/1‑150 (MQ=255)
                                                                                                                                     gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGGTGGACATGGGCAATc                >  2:2233390/1‑150 (MQ=255)
                                                                                                                                      ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg               >  2:3796226/1‑150 (MQ=255)
                                                                                                                                        gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg             >  1:3923140/1‑150 (MQ=255)
                                                                                                                                        gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg             <  2:2230970/150‑1 (MQ=255)
                                                                                                                                         ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg            <  1:669358/150‑1 (MQ=255)
                                                                                                                                          gggCAAGGCCTCCGGCACACAACATCACACCATCTCCAGCGCAGTTTGTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc           <  1:1631561/148‑1 (MQ=255)
                                                                                                                                          gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc           <  1:3273238/150‑1 (MQ=255)
                                                                                                                                          gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc           <  1:100330/150‑1 (MQ=255)
                                                                                                                                              aaTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCt       >  1:1936179/1‑150 (MQ=255)
                                                                                                                                               aTGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtt      >  1:579415/1‑150 (MQ=255)
                                                                                                                                                tGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCttt     <  1:526186/150‑1 (MQ=255)
                                                                                                                                                 gCATCCGGCACACAACATCACACCATCTCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtttt    <  1:2402437/150‑1 (MQ=255)
                                                                                                                                                 gCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCtttt    >  1:3088245/1‑150 (MQ=255)
                                                                                                                                                  cATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTg   >  2:33092/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGCCGCTTTTGG  >  NC_000913/1864621‑1864916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: