BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,876,923	C→T	6.4%	C5C (TGC→TGT)	yeaO →	DUF488 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,876,923	0	C	T	6.4%	625.7 / 21.3	186	C5C (TGC→TGT)	yeaO	DUF488 family protein
Reads supporting (aligned to +/- strand): ref base C (88/86); new base T (6/6); total (94/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 186 total aligned reads displayed.

CACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTT  >  NC_000913/1876775‑1877069

cACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCa                                                                                                                                                   >  1:3649522/1‑150 (MQ=255)

cACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCa                                                                                                                                                   >  1:2392907/1‑150 (MQ=255)

cACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCa                                                                                                                                                   >  1:2234202/1‑150 (MQ=255)

  cTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTaaa                                                                                                                                                 >  2:242826/1‑150 (MQ=255)

   ttATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAATGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAATGGGAGAACAAACACGATGAATATTCAGTGCAAAc                                                                                                                                                >  1:2605654/1‑150 (MQ=255)

     aTGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAAcgc                                                                                                                                              >  2:2856929/1‑150 (MQ=255)

        ggTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGtt                                                                                                                                           >  2:2824736/1‑150 (MQ=255)

        ggTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGtt                                                                                                                                           >  2:2824729/1‑150 (MQ=255)

         gtgtTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGttt                                                                                                                                          <  1:3209098/150‑1 (MQ=255)

           gtTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTAt                                                                                                                                        >  2:3422880/1‑150 (MQ=255)

            ttGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATg                                                                                                                                       <  2:3828757/150‑1 (MQ=255)

               ccATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGTGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTATCAAATCATGCGTGATAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATc                                                                                                                                    <  1:2094273/150‑1 (MQ=255)

               ccATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATc                                                                                                                                    >  2:1018750/1‑150 (MQ=255)

               ccATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATc                                                                                                                                    <  1:2836111/150‑1 (MQ=255)

               ccATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATc                                                                                                                                    <  1:2460152/150‑1 (MQ=255)

                cATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATcc                                                                                                                                   >  1:66305/1‑150 (MQ=255)

                 aTACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCg                                                                                                                                  >  2:1337281/1‑150 (MQ=255)

                  tACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCgg                                                                                                                                 >  1:1894123/1‑150 (MQ=255)

                  tACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCgg                                                                                                                                 <  2:1780516/150‑1 (MQ=255)

                  tACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCgg                                                                                                                                 <  1:658788/150‑1 (MQ=255)

                   aCTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGc                                                                                                                                <  1:763434/150‑1 (MQ=255)

                   aCTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGc                                                                                                                                <  2:1862213/150‑1 (MQ=255)

                    cTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCt                                                                                                                               <  1:3488062/150‑1 (MQ=255)

                    cTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCt                                                                                                                               <  1:13032/150‑1 (MQ=255)

                         actactGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCACACGCGTTTATGATCCGGCTGAACa                                                                                                                          >  2:210903/1‑150 (MQ=255)

                           tactGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACaga                                                                                                                        >  2:430815/1‑150 (MQ=255)

                           tactGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACaga                                                                                                                        >  2:2052924/1‑150 (MQ=255)

                                aTGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGAt                                                                                                                   <  2:2550456/150‑1 (MQ=255)

                                 tGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGATTATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATg                                                                                                                  <  2:1319286/150‑1 (MQ=255)

                                 tGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATg                                                                                                                  <  1:334773/150‑1 (MQ=255)

                                  ggCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg                                                                                                                 >  2:3418247/1‑150 (MQ=255)

                                  ggCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATAACCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATgg                                                                                                                 >  1:188615/1‑150 (MQ=255)

                                   gCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGt                                                                                                                >  2:1689671/1‑150 (MQ=255)

                                      aTTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTAt                                                                                                             <  1:3585100/150‑1 (MQ=255)

                                        tttCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCg                                                                                                           <  1:2705668/150‑1 (MQ=255)

                                          tCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCa                                                                                                         >  2:4266731/1‑150 (MQ=255)

                                            ggACATTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATa                                                                                                       <  2:1628253/150‑1 (MQ=255)

                                             gACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCAAGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATAc                                                                                                      >  1:585827/1‑150 (MQ=255)

                                                 ttGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGt                                                                                                  >  2:1211823/1‑150 (MQ=255)

                                                     gcCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGAc                                                                                              <  1:2725830/150‑1 (MQ=255)

                                                      ccGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGAcc                                                                                             >  1:1056357/1‑150 (MQ=255)

                                                      ccGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGAcc                                                                                             >  1:1056203/1‑150 (MQ=255)

                                                       cGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCg                                                                                            >  2:643436/1‑150 (MQ=255)

                                                        gggAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGc                                                                                           <  1:2024422/150‑1 (MQ=255)

                                                           agagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACATATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCctc                                                                                        <  2:3942145/150‑1 (MQ=255)

                                                            gagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCctct                                                                                       <  1:4021837/150‑1 (MQ=255)

                                                            gagaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCctct                                                                                       <  1:1874401/150‑1 (MQ=255)

                                                             agaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTg                                                                                      <  1:632000/150‑1 (MQ=255)

                                                             agaCAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTg                                                                                      >  1:1708322/1‑150 (MQ=255)

                                                                 aaaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcg                                                                                  >  1:2680400/1‑150 (MQ=255)

                                                                 aaaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcg                                                                                  >  1:3455492/1‑150 (MQ=255)

                                                                  aaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgc                                                                                 >  1:1886936/1‑150 (MQ=255)

                                                                  aaGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgc                                                                                 >  2:3072205/1‑150 (MQ=255)

                                                                     aaaTTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgcgcg                                                                              >  2:2159158/1‑150 (MQ=255)

                                                                     aaaTTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCcgcgcg                                                                              >  2:2159159/1‑150 (MQ=255)

                                                                        ttCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTAAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTa                                                                           <  1:713168/150‑1 (MQ=255)

                                                                         tCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTAt                                                                          <  1:964671/150‑1 (MQ=255)

                                                                         tCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTAt                                                                          >  2:4182301/1‑150 (MQ=255)

                                                                           gCTAATATCCGGTGCTATAGTGACGTATCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCa                                                                        >  1:533191/1‑150 (MQ=255)

                                                                             tAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaa                                                                      <  1:2074502/150‑1 (MQ=255)

                                                                                tatCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCaaaaaa                                                                   <  2:885243/150‑1 (MQ=255)

                                                                                     ggTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGTAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGa                                                              >  1:2520731/1‑150 (MQ=255)

                                                                                     ggTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGa                                                              >  1:3045423/1‑150 (MQ=255)

                                                                                      gTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGAt                                                             <  2:3200464/150‑1 (MQ=255)

                                                                                                 aCGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtga                                                  <  2:3663939/150‑1 (MQ=255)

                                                                                                  cGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtgat                                                 <  1:820166/150‑1 (MQ=255)

                                                                                                   gTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTtgatg                                                >  2:2993231/1‑150 (MQ=255)

                                                                                                     aaCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAg                                              >  1:297859/1‑150 (MQ=255)

                                                                                                     aaCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAg                                              >  2:1720728/1‑150 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             <  2:1787516/150‑1 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             <  1:1557496/150‑1 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             <  1:1981186/150‑1 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             >  1:3884795/1‑150 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             <  1:2885476/150‑1 (MQ=255)

                                                                                                      aCAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGt                                             <  1:698695/150‑1 (MQ=255)

                                                                                                       cAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTg                                            <  2:637924/150‑1 (MQ=255)

                                                                                                       cAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTg                                            <  2:814343/150‑1 (MQ=255)

                                                                                                        aaaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTgg                                           >  1:559989/1‑150 (MQ=255)

                                                                                                        aaaTCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTgg                                           >  1:2711628/1‑150 (MQ=255)

                                                                                                            cATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATa                                       <  2:4072300/150‑1 (MQ=255)

                                                                                                             aTGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATaa                                      <  1:4274842/150‑1 (MQ=255)

                                                                                                               gCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAg                                    >  1:2427047/1‑150 (MQ=255)

                                                                                                                     aaGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCa                              >  2:4190327/1‑150 (MQ=255)

                                                                                                                       gggAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACg                            <  1:4182301/150‑1 (MQ=255)

                                                                                                                         gagaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAATCCGATTTAGCCCTTGACGAGTGGGATAAAGAAATAACGcc                          >  2:3514952/1‑150 (MQ=255)

                                                                                                                         gagaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGcc                          >  1:3507479/1‑150 (MQ=255)

                                                                                                                          agaACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCg                         >  2:3981573/1‑150 (MQ=255)

                                                                                                                                      aTGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGTAATCACGCCGTCAACGGAACTg             >  2:2473164/1‑150 (MQ=255)

                                                                                                                                       tGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGTATAAAGAAATCACGCCGTCAACGGAACTgc            <  1:212058/150‑1 (MQ=255)

                                                                                                                                       tGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgc            <  1:3758848/150‑1 (MQ=255)

                                                                                                                                       tGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAAACACGCCGTCAACGGAACTgc            <  2:2527149/150‑1 (MQ=255)

                                                                                                                                        gAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgcg           <  2:1031763/150‑1 (MQ=255)

                                                                                                                                        gAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTgcg           <  2:1719481/150‑1 (MQ=255)

                                                                                                                                          atatTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCa         >  1:4070145/1‑150 (MQ=255)

                                                                                                                                          atatTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCa         >  1:1150847/1‑150 (MQ=255)

                                                                                                                                          atatTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCa         >  1:4195523/1‑150 (MQ=255)

                                                                                                                                            atTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCaaa       <  2:269040/150‑1 (MQ=255)

                                                                                                                                            atTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCaaa       <  1:3380236/150‑1 (MQ=255)

                                                                                                                                             ttCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAg      >  1:2194779/1‑150 (MQ=255)

                                                                                                                                                 gTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCtt  >  1:2940135/1‑150 (MQ=255)

CACTTATGGGTGTTGCCATACTTTCACTACTGATGGCGATTTTCGGACTTTGCGCCGGGAGAGACAAAGAAATTCGCTAATATCCGGTGCTATAGTGACGTAACAAATCATGCGTGAAAGGGAGAACAAACACGATGAATATTCAGTGCAAACGCGTTTATGATCCGGCTGAACAGAGCGATGGTTATCGCATACTGGTCGACCGCCTCTGGCCGCGCGGTATCAAAAAAACCGATTTAGCCCTTGATGAGTGGGATAAAGAAATCACGCCGTCAACGGAACTGCGCAAAGCCTT  >  NC_000913/1876775‑1877069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`