Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 21.8% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA21.8% 413.8 / 30.2 205intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (58/92);  new base A (34/17);  total (96/109)
Fisher's exact test for biased strand distribution p-value = 6.20e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383
                                                                                                                                                    |                                                                                                                                                     
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      <  1:3154352/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                     <  2:830542/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                     <  2:767282/150‑1 (MQ=255)
  ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                    <  2:1606020/150‑1 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                   <  2:2008243/150‑1 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                 <  2:1899901/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  2:2082225/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               >  2:693861/1‑150 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATg                                                                                                                                            >  2:2267455/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGaaaa                                                                                                                                        >  1:1467554/1‑149 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      >  2:2384599/1‑150 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      >  1:898689/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAattat                                                                                                                                     >  1:721704/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATCTTTCAAAATATAATTAAattat                                                                                                                                     >  1:4110278/1‑150 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATGAAattat                                                                                                                                     >  2:1880020/1‑150 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    >  2:2780775/1‑150 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   >  2:3195758/1‑150 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  1:1557717/150‑1 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  1:3873197/150‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAAAGAAATTAtttt                                                                                                                                  >  1:4158712/1‑149 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAGCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                >  2:360519/1‑150 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  2:601380/150‑1 (MQ=255)
                        tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                              <  2:3224863/150‑1 (MQ=255)
                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  2:898689/150‑1 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCAGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTa                                                                                                                            >  1:935300/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAAGAAAATATATTTTTTTaaa                                                                                                                          >  1:3688375/1‑150 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAatat                                                                                                                       >  2:4177492/1‑150 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAAAATTAAAATATTTTTTTTTAAataa                                                                                                                       >  1:1109615/1‑149 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTAAAATATTTTTTTTTAAatatat                                                                                                                     >  1:3961318/1‑148 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  1:2609439/150‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAAATATTTTTTTTTAAataaag                                                                                                                     >  2:1923292/1‑147 (MQ=255)
                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                 <  2:4266768/150‑1 (MQ=255)
                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                               <  1:435542/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  2:810135/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  1:3195758/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  2:3228482/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  2:1724748/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGTGATAAAGAt                                                                                                           >  1:369529/1‑150 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAAAAAAGAAAATAATTGTTTTTAAAAAACGAGAGAAccaa                                                                                                           >  2:756727/1‑147 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       >  2:995373/1‑150 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATcaaa                                                                                                       >  1:543972/1‑148 (MQ=255)
                                                    aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  >  2:539448/1‑150 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACg                                                                                                 >  2:2705353/1‑150 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGAc                                                                                               >  1:2472071/1‑150 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                              >  2:338551/1‑150 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTTAAAATAGAGATAACGATAACAAAAAAGACaa                                                                                             >  2:3368719/1‑150 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAATATGaa                                                                                       >  2:1182841/1‑150 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAAAAAATTTTTTTTAAATATCGGTATAAAGATAACAAAAAAAACAAAATTaa                                                                                       >  2:2528625/1‑150 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAAAGAAATTATTTTTTTTTAAATATAGAGATAAAGATAAAAAAAACGAAAaaatttaa                                                                                      >  2:3063817/1‑146 (MQ=255)
                                                                    aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                  <  2:1563817/150‑1 (MQ=255)
                                                                        ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTTAATATAGTGATAAAGATAACAAAAAAGACAATAATAAAattattag                                                                              >  2:1325544/1‑148 (MQ=255)
                                                                         cgcgCTGTGCGTTGTACAGCATCTGCTTGGCCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                             <  2:3958607/150‑1 (MQ=255)
                                                                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTGAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAAAGAAAAAAATAAAATTATTAgaggtg                                                                         >  1:2126738/1‑148 (MQ=255)
                                                                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                         <  2:1651248/150‑1 (MQ=255)
                                                                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAAATAAAATATTTTTTTTTTAAAATAGAGATAAACAAAACAAAAAAGACAAAAATAAAAATATTAgaggag                                                                         >  1:5807/1‑150 (MQ=255)
                                                                              tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAAAGACAATATGAAAATTATTAGAGGAGt                                                                        >  2:2669873/1‑150 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  2:3903497/150‑1 (MQ=255)
                                                                                  cGTTGTTCAGCAACTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTACAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  2:287881/150‑1 (MQ=255)
                                                                                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTTAAAATAAAAATAAAAAATTTTTTTTTTAATATATAGATAAAGAAAAAAAAAAAAACAAAAATAAAAATATTATAGGAGTGAAAGGCaa                                                               >  1:1866785/1‑150 (MQ=255)
                                                                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATTAAGCTAAACAAAACCACAAAATGAAAATTATTAGGGGGGTGTAAGGGaaaaaa                                                           >  1:2686766/1‑150 (MQ=255)
                                                                                             ttCTGCTTTGTCGATACCCTTTAATTTTTCATATCGTGTGTTTTGTTTTCTTTTACAAAATATATTTAATTTTTTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:1185484/149‑1 (MQ=255)
                                                                                             attttcTTTGTCGATACCACTTACTTTTTAATATCCTGTCGTTTTTTTTCTTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATTTCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:3566598/145‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:1943640/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         >  2:3869945/1‑150 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:839512/150‑1 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:1391930/150‑1 (MQ=255)
                                                                                                tGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:683830/150‑1 (MQ=255)
                                                                                                tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:2514861/150‑1 (MQ=255)
                                                                                                  cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                    >  2:3179762/1‑150 (MQ=255)
                                                                                                         tacaCCACGAAATTTTACATACCCTGTCTTTTGTTTTCTATTAAAAAATATATTTAAATTTTTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGTGTTAAAGGCAAAAAAACGGCCTCCCGATa                                             <  1:3104657/147‑1 (MQ=255)
                                                                                                         gATACCACTTACTTTTTCATATCCTGTCGTTTTTTTTCTTTTTAAAAATATATTGAATTTTTTTTTTTTTAAATTTCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  1:631186/150‑1 (MQ=255)
                                                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:3204383/150‑1 (MQ=255)
                                                                                                              cACTTACATTTTCATATCCTTTCTTTTTTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACTATCACAAAAACGAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:2468329/150‑1 (MQ=255)
                                                                                                              cACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  2:1109615/150‑1 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATTAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGaa                                        >  2:435526/1‑150 (MQ=255)
                                                                                                                  tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAAATATTTGTTTTTAAAAATAGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGTAAGGCCAAAAAACGGCCTCCCGAAAGGGAAGCCg                                    >  2:493914/1‑150 (MQ=255)
                                                                                                                    cATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:22389/150‑1 (MQ=255)
                                                                                                                    atttttCATATCCTTTGTTTTTTTTTCTTTTTAAAAATTTATTTAAATTTTTTTTTTTTAAATTTCTATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:995952/148‑1 (MQ=255)
                                                                                                                    aaTTTTCATATCCTGTCTTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACTAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:995201/149‑1 (MQ=255)
                                                                                                                         taatatCCTGTCTTTTTTTTTCTATTTAAAAATATAATTATATTTTTTTTTTTTAAATATCTATATAACGATAAAAAAAACTACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:1325544/148‑1 (MQ=255)
                                                                                                                              tCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCACGATAGGGAAGCCGTAGCAAAGTgcg                        >  2:1763093/1‑150 (MQ=255)
                                                                                                                               ccTGTCTTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:2406107/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTTTTTTCGATTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      <  1:3783454/150‑1 (MQ=255)
                                                                                                                                 tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcgg                     >  2:2547510/1‑149 (MQ=255)
                                                                                                                                    cGTTTGTTTTCGATTTCAAATTATATTGAAATTTTTTGTTTTTAATTATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGACAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAACGTGCGCGTGtt                  <  1:2747865/150‑1 (MQ=255)
                                                                                                                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              <  2:935300/150‑1 (MQ=255)
                                                                                                                                        tgtttTCGAGTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACATAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              <  2:2807337/150‑1 (MQ=255)
                                                                                                                                         gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:539448/150‑1 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  2:3146193/1‑150 (MQ=255)
                                                                                                                                           tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  1:3380937/1‑150 (MQ=255)
                                                                                                                                           tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  1:2056126/1‑150 (MQ=255)
                                                                                                                                             tCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:1156560/150‑1 (MQ=255)
                                                                                                                                             tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:2120262/150‑1 (MQ=255)
                                                                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg        >  1:1587022/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:3299189/1‑150 (MQ=255)
                                                                                                                                               gATTACAAAATATAATTAATTTATTTGTTTTTAAATATCGAGATAACGATAACAAACTCGACAATATTAAAATTATTCGTGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCTGa       <  1:151712/150‑1 (MQ=255)
                                                                                                                                                  ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc    >  1:3070043/1‑150 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:1445252/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:2954703/1‑150 (MQ=255)
                                                                                                                                                    cAAAATAAAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  1:3528863/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: