Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 580,727 G→T 6.3% intergenic (‑282/‑107) ylcI ← / → nohD DUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,7270GT 6.3% 606.3 / 3.8 187intergenic (‑282/‑107)ylcI/nohDDUF3950 family protein, DLP12 prophage/DLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base G (102/65);  new base T (2/18);  total (104/83)
Fisher's exact test for biased strand distribution p-value = 1.42e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

GTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580579‑580876
                                                                                                                                                    |                                                                                                                                                     
  tgtgACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAGATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtat                                                                                                                                                    <  2:952100/150‑1 (MQ=255)
    tgACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtattt                                                                                                                                                  >  1:836264/1‑150 (MQ=255)
    tgACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtattt                                                                                                                                                  >  2:747992/1‑150 (MQ=255)
    tgACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAAATTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtattt                                                                                                                                                  <  2:1314229/150‑1 (MQ=255)
       ccacaaaatgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttatt                                                                                                                                               >  1:2547081/8‑150 (MQ=255)
       cATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttatt                                                                                                                                               <  2:480593/150‑1 (MQ=255)
        aTGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTatttattt                                                                                                                                              >  2:1213949/1‑150 (MQ=255)
         tGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTAtttattta                                                                                                                                             <  1:948715/150‑1 (MQ=255)
          gTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTGGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTGGTAACTTAATGTAtttatttat                                                                                                                                            >  1:1099631/1‑149 (MQ=255)
           tAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaaa                                                                                                                                           <  2:3898968/150‑1 (MQ=255)
            aGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTaaaa                                                                                                                                          <  2:2511838/150‑1 (MQ=255)
             gatgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAAt                                                                                                                                         >  1:1707972/1‑150 (MQ=255)
               tgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAc                                                                                                                                       <  1:2067748/150‑1 (MQ=255)
               tgatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAc                                                                                                                                       <  2:2946297/150‑1 (MQ=255)
                gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      >  1:572821/1‑150 (MQ=255)
                gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      <  1:4170840/150‑1 (MQ=255)
                gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      <  1:4165618/150‑1 (MQ=255)
                gatAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAcc                                                                                                                                      >  2:2987125/1‑150 (MQ=255)
                 atAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccc                                                                                                                                     <  2:1680754/150‑1 (MQ=255)
                 atAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAccc                                                                                                                                     <  2:2781216/150‑1 (MQ=255)
                     ttattaTCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTg                                                                                                                                 <  2:2984134/150‑1 (MQ=255)
                       attatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaa                                                                                                                               >  1:1923735/1‑150 (MQ=255)
                        ttatCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGaaa                                                                                                                              >  1:931616/1‑150 (MQ=255)
                           tCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaaga                                                                                                                           <  2:2660136/150‑1 (MQ=255)
                           tCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaaga                                                                                                                           >  2:1279563/1‑150 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:3174253/150‑1 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:1710055/150‑1 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  2:3662453/150‑1 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:1288403/150‑1 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:692895/150‑1 (MQ=255)
                             aTTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaa                                                                                                                         <  1:4252441/150‑1 (MQ=255)
                              ttttGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaag                                                                                                                        >  1:504597/1‑150 (MQ=255)
                              ttttGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAaaagaaag                                                                                                                        <  1:1839042/150‑1 (MQ=255)
                                      gTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTCTTTAAAATACCCCCTGAAAAGAAAGGAAACGAc                                                                                                                <  2:563656/150‑1 (MQ=255)
                                       tCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttat                                                                                                                                                >  2:200026/1‑117 (MQ=255)
                                       tCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGtatttat                                                                                                                                                <  1:200026/117‑1 (MQ=255)
                                          tttCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGt                                                                                                            >  1:2903165/1‑150 (MQ=39)
                                          tttCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTAGTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGt                                                                                                            >  2:950240/1‑150 (MQ=255)
                                               ngCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTCTTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGa                                                                                                       >  1:1606133/2‑150 (MQ=255)
                                                    tCCGACTGGTTCCGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAATTTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGCAAACGACAGGTGCTGAAAGCg                                                                                                  <  2:1324175/150‑1 (MQ=255)
                                                    tCCGACAGGTTACGGGGCGGCGACCTCGGGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCg                                                                                                  <  2:253621/150‑1 (MQ=255)
                                                      cGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAg                                                                                                >  2:422969/1‑150 (MQ=39)
                                                      cGACAGGTTACGGGGCGGAGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTTTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAATGAAACGACAGGTGCTGAAAGCGAg                                                                                                >  2:694026/1‑150 (MQ=255)
                                                       gACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGc                                                                                               >  2:1768174/1‑150 (MQ=39)
                                                       gACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGc                                                                                               >  2:1581462/1‑150 (MQ=39)
                                                       gACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGc                                                                                               >  2:3544741/1‑150 (MQ=39)
                                                             ttACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTg                                                                                         >  1:2867615/1‑150 (MQ=40)
                                                               ccGGGGCGGCGACCTCGGGGTTTTTCTCTTTTTATTAAATTTTTCCGGTTTATGGTTTTTCGTTTCTTCTTCGTCTTAAATTATTTTATTTATTAAAAATCCCCCCTTAAAACAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGc                                                                                       <  2:1529044/149‑1 (MQ=255)
                                                                      ggcgCCCTCGCGGGTTTGCGCTATTTATGAAAATTTTCCGGTTTCAGGTGTTGCCGTTCTTCTTCGTCGTACCTTAATGTATTTATTTAAAATACCCCCTGAAACGACAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTc                                                                                <  2:4159541/150‑1 (MQ=14)
                                                                        cgACCTCGGGGGTTTTCGATTTTTATGAAAATTTTCCGGTTTATGGTGTTTCCGTTCTTCTTCGTCGTAACTTATTTTATTTATTTAAAATACCCCCTCAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGt                                                                              <  2:836264/150‑1 (MQ=14)
                                                                          aCCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGttt                                                                            >  2:833850/1‑150 (MQ=255)
                                                                             tCGGGGGTTTTCGCTATTTTTTAAATTTTGCCGGTTTAAGGTGTTTCGGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTCAACATAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         <  2:1507719/150‑1 (MQ=14)
                                                                             tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         >  2:540380/1‑150 (MQ=255)
                                                                             tCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTAGTCGTAACTTAATTTATTTATTTAAAATAACCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCATTTTTGCCTCTGTCGTTTACt                                                                         >  1:1176772/1‑150 (MQ=14)
                                                                             ccgcgGGTTTTCGCTATTTTTGAAAATTTTCCTGTTTATGGTGTTTCGGTTCTCCTTCGACGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCt                                                                         <  2:3318921/149‑1 (MQ=14)
                                                                               gcgGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCttt                                                                       >  2:2413284/1‑150 (MQ=255)
                                                                                cgGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtc                                                                      >  1:3522110/1‑150 (MQ=255)
                                                                                 gggTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtct                                                                     >  2:3761448/1‑150 (MQ=255)
                                                                                 gggTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGACTCTGTCGTTTCCtttat                                                                     >  2:1956250/1‑148 (MQ=255)
                                                                                  ttttttCTCTTTTTTTTAAAATTTGCGGGTTTATGGTTTTACGGTTCTTCTTCTTCTTAAATTATTTTATTAATTTAAAAAACCCCCTCAAAACAACGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTtctc                                                                    <  1:2824773/148‑1 (MQ=9)
                                                                                   gTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTctct                                                                   >  1:4039805/1‑150 (MQ=255)
                                                                                   gTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTctct                                                                   >  1:2714387/1‑150 (MQ=255)
                                                                                     tttCGCTATTTATTAAAATTTTCCGTTTGATGGTGTTTCCGTTCTTCTTCGACGTAAATTAATGTATTTATTTAAAATACCCCCTTAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                 <  2:405444/150‑1 (MQ=14)
                                                                                     tttCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGt                                                                 >  1:2191399/1‑150 (MQ=255)
                                                                                       tctatttttATTAATATTTTGTGTTTTATGGTTTTCCTTTTTTTCTTCTTCTAAAATTATTTTATTTATTAAAAAAACCCCCTCAAAACAACGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGttt                                                               <  1:4058486/144‑1 (MQ=9)
                                                                                        cGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATAAACCCTTAAAAGAAAGTAAAAGACAGGTGATGAAATCGAGCTTTTTGGACTCTGTCGTTTCCTTTATCTGtttt                                                              >  1:833440/1‑150 (MQ=14)
                                                                                         gCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGttttt                                                             >  1:1367230/1‑150 (MQ=255)
                                                                                            aTTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTc                                                          <  2:1384972/150‑1 (MQ=255)
                                                                                             tttATTAAATTTTTCCTTTTTATGGTTTTTCCTTTCTTCTTGTTCGTAAGTTAATTTATTTATTTAAATTACCCCCTTAAAATAAATCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTcc                                                         <  2:4135394/150‑1 (MQ=14)
                                                                                             tttATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTcc                                                         >  1:621879/1‑150 (MQ=255)
                                                                                                aTGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTg                                                      <  2:2409482/150‑1 (MQ=255)
                                                                                                aTGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTg                                                      >  2:2623072/1‑150 (MQ=255)
                                                                                                 tGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTgg                                                     >  1:3477928/1‑150 (MQ=255)
                                                                                                    attttttCCGGTTTTTGTTTTTTCGTTTATTATTCTTCTTAATTTTTTTTATTTATTTAAAATACCCCATTAAAATAAAGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAt                                                  <  1:747992/147‑1 (MQ=9)
                                                                                                       ttttCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAAtgtg                                               <  2:1780187/150‑1 (MQ=255)
                                                                                                          tCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGATGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCaa                                            >  2:2130581/1‑150 (MQ=255)
                                                                                                           ccGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAAt                                           >  2:429778/1‑150 (MQ=255)
                                                                                                             gttttATGGTTTTTCCTTTCTTATTCTTCTTAAATTAATTTATTTATTAAAAAAACCCCCTTAAAATAACGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATgg                                         <  1:1555997/148‑1 (MQ=14)
                                                                                                             ggTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATgg                                         >  2:2866978/1‑150 (MQ=255)
                                                                                                              gTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCGTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGa                                        >  1:1969048/1‑150 (MQ=255)
                                                                                                               tttAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTTTTTATTTAATATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGaa                                       <  2:1086903/150‑1 (MQ=255)
                                                                                                                    ggTGTTTCCGTTCTTCTTGGTCGTAACTTAATTTTTTTATTTAAAATCCCCCCGGAAAAGAACGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaa                                  <  2:1619652/150‑1 (MQ=14)
                                                                                                                     gtttttCCGTTATTCTTCGTCTTAAATTATTTTATTTATTTAAAATACCCCCTGAAAACAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaac                                 <  2:1523261/147‑1 (MQ=14)
                                                                                                                     gtgtTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTcaac                                 <  2:709126/150‑1 (MQ=255)
                                                                                                                          tCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCATTCTCTGTTTTTGTCCCTGGAATGTGCAATGGAAGTCAACaaaaa                            >  2:3595557/1‑150 (MQ=255)
                                                                                                                           ccGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAag                           >  1:2478974/1‑150 (MQ=255)
                                                                                                                              ttcttcttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagcag                        >  1:655146/1‑150 (MQ=255)
                                                                                                                               tcttcttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAagcagc                       >  1:2392626/1‑150 (MQ=255)
                                                                                                                                    ttcGTCTTAATTTATTTTATTTATTTAAAACCCCCCCTCAAAATAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggct                  <  2:1823800/150‑1 (MQ=14)
                                                                                                                                    ttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggct                  >  1:3536431/1‑150 (MQ=255)
                                                                                                                                    ttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggct                  >  2:2610371/1‑150 (MQ=255)
                                                                                                                                    ttcGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggct                  <  2:610683/150‑1 (MQ=255)
                                                                                                                                    ttcGGCGTAAATAATTTTATTTATCTAAAATACCCCCTGAACACAAAGCAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggct                  <  2:2059308/150‑1 (MQ=14)
                                                                                                                                     tgttctaaaCTTAATGTATTTATTAAAAACACCCCATTAAAAGAATGTAAACGACTGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggctg                 <  1:2652949/143‑1 (MQ=14)
                                                                                                                                     acgtcttAAATAATTGTATTTATTAAAAATACCCCCTCAAAATAAAGCACACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAgctggctg                 <  1:2652979/149‑1 (MQ=14)
                                                                                                                                       gtcgtAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGAc               >  1:3405492/1‑150 (MQ=255)
                                                                                                                                            aaattAATTTTTTTATTAAAAATACCCCCTCAAAACAAAGTAAACGACAGGTGCTGAAAGCGAGCTTTTGGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAtttt          <  1:3868048/147‑1 (MQ=11)
                                                                                                                                            aaCTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACAtttt          >  1:3914860/1‑150 (MQ=255)
                                                                                                                                               ttAATGTATTTATTTAAAATACCCCATCTAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCgg       <  2:3260139/150‑1 (MQ=255)
                                                                                                                                                    gTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGa  <  2:247422/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTTGTGACATGTAGATGATAATTATTATCATTTTGTGGGTCCTTTCCGGCGATCCGACAGGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGTGTTTCCGTTCTTCTTCGTCGTAACTTAATGTATTTATTTAAAATACCCCCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGTGCAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGA  >  NC_000913/580579‑580876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: