Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,566,266 C→A 5.1% G207C (GGC→TGC)  eutA ← reactivating factor for ethanolamine ammonia lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,566,2660CA 5.1% 678.9 / 18.0 186G207C (GGC→TGC) eutAreactivating factor for ethanolamine ammonia lyase
Reads supporting (aligned to +/- strand):  ref base C (91/85);  new base A (2/8);  total (93/93)
Fisher's exact test for biased strand distribution p-value = 9.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

GGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566118‑2566414
                                                                                                                                                    |                                                                                                                                                    
ggCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     <  2:2791240/150‑1 (MQ=255)
ggCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCt                                                                                                                                                     <  2:526584/150‑1 (MQ=255)
 gCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTg                                                                                                                                                    <  2:3143139/150‑1 (MQ=255)
   gTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCa                                                                                                                                                  >  1:76162/1‑150 (MQ=255)
    tAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCAc                                                                                                                                                 <  2:1750368/150‑1 (MQ=255)
    tAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCAc                                                                                                                                                 <  2:1982079/150‑1 (MQ=255)
    tAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCAc                                                                                                                                                 <  1:2337936/150‑1 (MQ=255)
      acacCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCg                                                                                                                                               <  1:2443961/150‑1 (MQ=255)
        acCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGaa                                                                                                                                             <  1:1396829/150‑1 (MQ=255)
        acCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGaa                                                                                                                                             <  1:3836185/150‑1 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGACGccc                                                                                                                                         <  2:3263154/150‑3 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGACGccc                                                                                                                                         <  1:2656034/150‑3 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGACGccc                                                                                                                                         <  2:3303962/150‑3 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCAc                                                                                                                                         <  2:3017530/150‑1 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAATGCCGGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGACGccc                                                                                                                                         <  2:336149/150‑3 (MQ=255)
            gCGGGCAGCAAACCGGTTTGCATCAAGGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCTTCGGTGCAGGCACCGACGccc                                                                                                                                         <  1:150948/150‑3 (MQ=255)
                gcagcaAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCAt                                                                                                                                     >  1:3089181/1‑150 (MQ=255)
                  agcaAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATcc                                                                                                                                   >  1:3005935/1‑150 (MQ=255)
                     aaaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCcaca                                                                                                                                <  1:362637/150‑1 (MQ=255)
                     aaaCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCcaca                                                                                                                                <  1:1906120/150‑1 (MQ=255)
                       aCCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAat                                                                                                                              <  2:3781481/150‑1 (MQ=255)
                           gTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatcatc                                                                                                                          >  1:2048470/1‑150 (MQ=255)
                           gTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAatcatc                                                                                                                          <  2:1785460/150‑1 (MQ=255)
                            tttGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCt                                                                                                                         >  1:3209064/1‑150 (MQ=255)
                                cATCAATGCCTGCGCGAGCGGTGAGAGCGGTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGccc                                                                                                                     >  1:1356288/1‑150 (MQ=255)
                                 aTCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGcccc                                                                                                                    >  1:588350/1‑150 (MQ=255)
                                  tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCg                                                                                                                   >  1:334011/1‑150 (MQ=255)
                                  tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCg                                                                                                                   >  1:240499/1‑150 (MQ=255)
                                  tCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAAGCATCTGCCCCg                                                                                                                   >  1:4093992/1‑150 (MQ=255)
                                  tCAAAGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCg                                                                                                                   >  1:711053/1‑150 (MQ=255)
                                   cAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCgg                                                                                                                  <  1:4036327/150‑1 (MQ=255)
                                    aaTGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGt                                                                                                                 >  1:2181996/1‑150 (MQ=255)
                                     aTGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGtt                                                                                                                >  2:3268030/1‑150 (MQ=255)
                                      tGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGttt                                                                                                               <  2:1986014/150‑1 (MQ=255)
                                      tGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGttt                                                                                                               <  2:148595/150‑1 (MQ=255)
                                        ccTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             <  2:1706950/150‑1 (MQ=255)
                                        ccTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             >  1:1999572/1‑150 (MQ=255)
                                        ccTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTAt                                                                                                             <  2:1706784/150‑1 (MQ=255)
                                          tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGa                                                                                                           <  1:1242608/150‑1 (MQ=255)
                                          tGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGa                                                                                                           <  1:3387409/150‑1 (MQ=255)
                                            cgcgAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGc                                                                                                         <  2:1032079/150‑1 (MQ=255)
                                            cgcgAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGc                                                                                                         <  2:749404/150‑1 (MQ=255)
                                            cgcgAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCAGCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGGCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGc                                                                                                         <  2:2534113/150‑1 (MQ=255)
                                                 gCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    >  1:541417/1‑150 (MQ=255)
                                                 gCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  1:1567379/150‑1 (MQ=255)
                                                 gCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  2:4116029/150‑1 (MQ=255)
                                                 gCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTaaa                                                                                                    <  1:227279/150‑1 (MQ=255)
                                                  cGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAAc                                                                                                   >  1:653923/1‑150 (MQ=255)
                                                   ggTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAAcc                                                                                                  >  1:1903012/1‑150 (MQ=255)
                                                     tGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCAc                                                                                                >  1:29540/1‑150 (MQ=255)
                                                      gagaGCGTTCAGTCAATCACTTCGACAATCATTTCTGCCATCCGCCGGGTCACCTGCAACAGCTGCGCGCCGGTCAGCGAACGGGCGTGGGTGCCTGCACGGAATCACTCATCCACAATCATCTGCCACGGTTTTTGAGCGTAAACCAcg                                                                                               <  1:2244049/150‑1 (MQ=255)
                                                        gagCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCAcgcg                                                                                             >  1:482324/1‑150 (MQ=255)
                                                          gCGTTCCGTCAATCACATCGACAATCAGTTCTGCCATACGCCGGGTCACCTGCACCAGCTGCGCGCCGGTAAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCAACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGcc                                                                                           >  1:3065521/1‑150 (MQ=255)
                                                              tCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTg                                                                                       <  1:2846012/150‑1 (MQ=255)
                                                               ccGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTgg                                                                                      >  2:29218/1‑150 (MQ=255)
                                                                cGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGGTTATGAGCGTAAACCACGCGCCCGTGGc                                                                                     >  1:1103798/1‑150 (MQ=255)
                                                                 gTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCt                                                                                    >  1:193326/1‑150 (MQ=255)
                                                                    aaTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTc                                                                                 >  1:2047033/1‑150 (MQ=255)
                                                                    aaTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTAACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCAAGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTc                                                                                 >  2:1899679/1‑150 (MQ=255)
                                                                     aTCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCg                                                                                >  1:1702706/1‑150 (MQ=255)
                                                                        aCTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGtt                                                                             >  2:118932/1‑150 (MQ=255)
                                                                           tCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACACTCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTcc                                                                          <  1:2116099/150‑1 (MQ=255)
                                                                             gACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcag                                                                        <  1:3736481/150‑1 (MQ=255)
                                                                             gACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcag                                                                        >  1:3762049/1‑150 (MQ=255)
                                                                               cAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagca                                                                      <  2:821227/150‑1 (MQ=255)
                                                                               cAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagca                                                                      <  2:1358470/150‑1 (MQ=255)
                                                                               cAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagca                                                                      <  1:2210533/150‑1 (MQ=255)
                                                                               cAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagca                                                                      <  1:2766779/150‑1 (MQ=255)
                                                                                aaTCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTAATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCcagcag                                                                     <  2:3857325/150‑1 (MQ=255)
                                                                                 aTCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAgg                                                                    >  2:131228/1‑150 (MQ=255)
                                                                                  tCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGc                                                                   <  2:1651998/150‑1 (MQ=255)
                                                                                  tCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGc                                                                   <  2:354265/150‑1 (MQ=255)
                                                                                     gTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGac                                                                <  2:1683873/150‑1 (MQ=255)
                                                                                       tCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGacca                                                              <  2:306236/150‑1 (MQ=255)
                                                                                         tGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACTGGCTTCGGTGCCTGCTCCGAAGCTCTCATCCAAAATCACCTGCCCGCGTTTATGAGCGTAAACCACGCGCCCGTTGCTTTCTGTTTGCAGCATGCGaccacc                                                            <  2:4162199/150‑1 (MQ=255)
                                                                                           ccATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCgc                                                          >  1:188845/1‑149 (MQ=255)
                                                                                           ccATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTGTCCAGCAGGCGACCCCCGa                                                          >  1:3978727/1‑150 (MQ=255)
                                                                                             aTCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACg                                                        <  2:1082308/150‑1 (MQ=255)
                                                                                                 gccgGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGa                                                    >  1:1017899/1‑150 (MQ=255)
                                                                                                 gccgGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGa                                                    >  1:1018086/1‑150 (MQ=255)
                                                                                                    gggTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggc                                                 >  2:2588461/1‑150 (MQ=255)
                                                                                                     ggTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTGCCAGCAGGCGACCACCGACGTTGaggca                                                >  1:503929/1‑150 (MQ=255)
                                                                                                      gTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGaggcag                                               <  2:2293319/150‑1 (MQ=255)
                                                                                                         aCCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGGTGAggcaggca                                            >  2:276230/1‑150 (MQ=255)
                                                                                                           cTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGt                                          >  1:2713845/1‑150 (MQ=255)
                                                                                                                ccAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGc                                     >  1:1581886/1‑150 (MQ=255)
                                                                                                                    cTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGAt                                 >  1:3684596/1‑150 (MQ=255)
                                                                                                                          gcCGGTCAGCGAACGGGCGTCAGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTcc                           >  1:2164536/1‑150 (MQ=255)
                                                                                                                                 aGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCg                    >  2:2423825/1‑150 (MQ=255)
                                                                                                                                      aCGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAg               >  1:2396490/1‑150 (MQ=255)
                                                                                                                                        gggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAggg             <  2:3089181/150‑1 (MQ=255)
                                                                                                                                        gggCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAggg             >  2:529042/1‑150 (MQ=255)
                                                                                                                                          gCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCg           <  2:3342100/150‑1 (MQ=255)
                                                                                                                                          gCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCg           >  2:323253/1‑150 (MQ=255)
                                                                                                                                            ttCGGTGCAGGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTa         >  1:3931130/2‑150 (MQ=255)
                                                                                                                                            gTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCCCTGATTTTTCCGGCATCGAACAGGGCGTa         >  1:3484035/1‑150 (MQ=255)
                                                                                                                                             tCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAg        >  1:2991410/1‑150 (MQ=255)
                                                                                                                                                gTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTc     <  2:1231136/150‑1 (MQ=255)
                                                                                                                                                gTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTc     >  1:113863/1‑150 (MQ=255)
                                                                                                                                                   ccTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTcgcg  <  2:2169549/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGTAACACCTGCGGGCAGCAAACCGGTTTGCATCAATGCCTGCGCGAGCGGTGAGAGCGTTCCGTCAATCACTTCGACAATCAGTTCTGCCATCCGCCGGGTCACCTGCACCAGCTGCGCGCCGGTCAGCGAACGGGCGTCGGTGCCTGCACCGAAGCACTCATCCACAATCATCTGCCCCGGTTTATGAGCGTAAACCACGCGCCCGTGGCTGTCGGTTTCCAGCAGGCGACCACCGACGTTGAGGCAGGCAGTGCCGCTGATTTTTCCGGCATCGAACAGGGCGTAGTTCGCG  >  NC_000913/2566118‑2566414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: