Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,821,424 G→C 53.3% G196G (GGC→GGG)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,821,4240GC53.3% 35.1 / 217.6 122G196G (GGC→GGG) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (26/31);  new base C (20/45);  total (46/76)
Fisher's exact test for biased strand distribution p-value = 9.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CAGCAATACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCC  >  NC_000913/2821276‑2821569
                                                                                                                                                    |                                                                                                                                                 
cagcaATACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGc                                                                                                                                                  <  1:743364/150‑1 (MQ=255)
 cgcaATACGCTCCAGACCCCGACCGGGATCTACCGACGGCGTCGGCAGCGGGTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCACTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGccc                                                                                                                                                 <  2:4282201/149‑1 (MQ=255)
 agcaATACGCTCCAGACCCATACCGGTATCTACAGCCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGccc                                                                                                                                                 <  2:4282149/150‑1 (MQ=255)
 agcaATACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGccc                                                                                                                                                 <  2:2247398/150‑1 (MQ=255)
  gcaATACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccc                                                                                                                                                <  2:773747/150‑1 (MQ=255)
   ccaTACGCCCCCGCCCCCCACCGGCACCCACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGcccc                                                                                                                                               <  1:2672376/148‑1 (MQ=255)
   caATACGCTCCAGACCCATCCCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCcccc                                                                                                                                               <  2:3257495/150‑1 (MQ=255)
    aaTACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGcccccc                                                                                                                                              <  2:107511/150‑1 (MQ=255)
        cGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGATGACGATGTTCCAGATCTCAATGTAGCGGTCGCCTTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCaa                                                                                                                                          <  1:797345/150‑1 (MQ=255)
           tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAAtg                                                                                                                                       >  2:518330/1‑150 (MQ=255)
           tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAAtg                                                                                                                                       >  2:518266/1‑150 (MQ=255)
           tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtg                                                                                                                                       >  2:3274908/1‑150 (MQ=255)
            ccAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt                                                                                                                                      <  2:2030943/150‑1 (MQ=255)
            ccAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAAtgt                                                                                                                                      <  1:1318164/150‑1 (MQ=255)
             cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAAtgtg                                                                                                                                     >  2:1976902/1‑150 (MQ=255)
                aCCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTc                                                                                                                                  >  2:1960094/1‑150 (MQ=255)
                aCCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTc                                                                                                                                  <  1:2233540/150‑1 (MQ=255)
                  ccATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGc                                                                                                                                >  2:2607145/1‑150 (MQ=255)
                  ccATACCGGTATCTACAGACGGCTTCGGCAGCGGTACCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTACCCGGAGGGCCCCCCCAAATGTGGTCGc                                                                                                                                >  2:2607315/1‑150 (MQ=255)
                    aTACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCg                                                                                                                              >  2:606648/1‑150 (MQ=255)
                    aTACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCg                                                                                                                              >  2:2096861/1‑150 (MQ=255)
                       ccGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGa                                                                                                                           <  1:3457559/150‑1 (MQ=255)
                        cGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCTGGAGGGCCCCCCCAAATGTGGTCGCCGt                                                                                                                             >  1:308146/1‑147 (MQ=255)
                        cGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCTGGAGGGCCCCCCCAAATGTGGTCGCCGt                                                                                                                             >  1:308064/1‑147 (MQ=255)
                        cGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCTGGAGGGCCCCCCCAAATGTGGTCGCCGt                                                                                                                             <  2:308064/147‑1 (MQ=255)
                        cGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCTGGAGGGCCCCCCCAAATGTGGTCGCCGt                                                                                                                             <  2:308146/147‑1 (MQ=255)
                        cGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGAt                                                                                                                          >  2:2778733/1‑150 (MQ=255)
                           tATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt                                                                                                                       <  1:67094/150‑1 (MQ=255)
                           tATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGt                                                                                                                       <  1:66631/150‑1 (MQ=255)
                            aTCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTa                                                                                                                      <  1:2805279/150‑1 (MQ=255)
                             tCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                                     <  1:1979599/150‑1 (MQ=255)
                               tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                                                                                   <  2:3673715/150‑1 (MQ=255)
                                  aGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGACCTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGGGATCGGAGAAGAt                                                                                                                >  2:1015741/1‑150 (MQ=255)
                                  aGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                                                                                >  2:760502/1‑150 (MQ=255)
                                  aGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                                                                                >  2:2462653/1‑150 (MQ=255)
                                  aGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAt                                                                                                                >  2:4023993/1‑150 (MQ=255)
                                      ggCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCg                                                                                                            <  1:362600/150‑1 (MQ=255)
                                       gCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCgg                                                                                                           <  2:2718756/150‑1 (MQ=255)
                                         ttCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTg                                                                                                         >  2:2799805/1‑150 (MQ=255)
                                           cGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                                                                                       <  1:3574288/150‑1 (MQ=255)
                                             gCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTCGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACg                                                                                                     >  2:3389368/1‑150 (MQ=255)
                                               agaggTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGAGGTCGCAGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCTTAGAAGTTTTCTGTGCAAggg                                                                                                   >  2:3768530/4‑150 (MQ=255)
                                                gCGGGTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                                                                                  <  1:4219546/150‑1 (MQ=255)
                                                gCGGGTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGc                                                                                                  <  1:2440590/150‑1 (MQ=255)
                                                    ttCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                              >  1:1552053/1‑150 (MQ=255)
                                                    ttCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                              <  1:3337438/150‑1 (MQ=255)
                                                    ttCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                              <  1:2155608/150‑1 (MQ=255)
                                                         tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                                         <  2:587891/150‑1 (MQ=255)
                                                         tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                                         >  2:2712341/1‑150 (MQ=255)
                                                         tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                                         <  2:1237811/150‑1 (MQ=255)
                                                           gTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCa                                                                                       <  2:394659/150‑1 (MQ=255)
                                                            tGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGTAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                                      <  2:271734/150‑1 (MQ=255)
                                                            tGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                                      <  2:271756/150‑1 (MQ=255)
                                                             gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgt                                                                                     <  1:548394/150‑1 (MQ=255)
                                                             gCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgt                                                                                     <  1:4244901/150‑1 (MQ=255)
                                                                       tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATc                                                                           <  1:1937888/150‑1 (MQ=255)
                                                                       tGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGA‑GGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                                                                          >  2:688812/1‑150 (MQ=255)
                                                                        ggCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                                                                          >  2:4108196/1‑150 (MQ=255)
                                                                        ggCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCt                                                                          >  2:2311352/1‑150 (MQ=255)
                                                                            gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCa                                                                      <  2:777515/150‑1 (MQ=255)
                                                                            gTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCa                                                                      <  2:143324/150‑1 (MQ=255)
                                                                               gAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                                   <  2:2152278/150‑1 (MQ=255)
                                                                                   tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTg                                                               <  1:2462653/150‑1 (MQ=255)
                                                                                    gCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGt                                                              <  1:476528/150‑1 (MQ=255)
                                                                                    gCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGA‑GGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc                                                             <  1:3299611/150‑1 (MQ=255)
                                                                                     cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc                                                             <  1:2722501/150‑1 (MQ=255)
                                                                                     cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc                                                             <  1:1960094/150‑1 (MQ=255)
                                                                                      aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa                                                            <  1:1272621/150‑1 (MQ=255)
                                                                                      aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa                                                            <  1:1961865/150‑1 (MQ=255)
                                                                                      aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCa                                                            <  1:135472/150‑1 (MQ=255)
                                                                                           gACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCACATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGc                                                       <  1:3245076/150‑1 (MQ=255)
                                                                                                   tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                               >  2:1405265/1‑150 (MQ=255)
                                                                                                     cAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCa                                             >  1:3864318/1‑150 (MQ=255)
                                                                                                      aGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCAc                                            >  2:547081/1‑150 (MQ=255)
                                                                                                        aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTag                                                                                                                     <  1:45831/75‑1 (MQ=255)
                                                                                                        aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                                         >  1:411302/1‑103 (MQ=255)
                                                                                                        aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                                         <  2:411302/103‑1 (MQ=255)
                                                                                                          ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttt                                        >  1:4283216/1‑150 (MQ=255)
                                                                                                          ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTGCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttt                                        <  1:1586755/150‑1 (MQ=255)
                                                                                                           tcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTg                                       <  1:4023993/150‑1 (MQ=255)
                                                                                                            cAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt                                      <  2:148095/150‑1 (MQ=255)
                                                                                                            cAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGt                                      <  1:3147341/150‑1 (MQ=255)
                                                                                                            cAATGTAGCGGTCGCCGACTACTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCttttt                                      <  1:2442389/150‑3 (MQ=255)
                                                                                                                     ggTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGa                             <  1:1926941/150‑1 (MQ=255)
                                                                                                                       tCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg                           <  1:1029179/150‑1 (MQ=255)
                                                                                                                       tCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATg                           <  1:90359/150‑1 (MQ=255)
                                                                                                                             tcttctTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaata                     <  1:3369297/150‑1 (MQ=255)
                                                                                                                              cttcttCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaataa                    <  2:2069825/150‑1 (MQ=255)
                                                                                                                               ttcttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaataat                   >  2:1528843/1‑150 (MQ=255)
                                                                                                                                  ttcCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACg                >  2:4223531/1‑150 (MQ=255)
                                                                                                                                      gggCTTCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcg            >  2:524264/1‑150 (MQ=255)
                                                                                                                                         cTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgc         <  1:606648/150‑1 (MQ=255)
                                                                                                                                          ttCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg        >  1:2093035/1‑150 (MQ=255)
                                                                                                                                          ttCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg        >  1:2092917/1‑150 (MQ=255)
                                                                                                                                          ttCCCGGAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTcgcgcg        >  2:3618085/1‑150 (MQ=255)
                                                                                                                                              cGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGAt    <  2:2159176/150‑1 (MQ=255)
                                                                                                                                               ggAGGCCCCCCCCAACTGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATc   <  1:4052881/150‑1 (MQ=255)
                                                                                                                                               ggAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATc   <  1:4052767/150‑1 (MQ=255)
                                                                                                                                                gAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATcc  <  2:3265544/150‑1 (MQ=255)
                                                                                                                                                gAGGCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATcc  <  1:944685/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGCAATACGCTCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGGCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTCGCGCGGGATCC  >  NC_000913/2821276‑2821569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑