Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,118 T→G 5.7% noncoding (72/88 nt) omrA ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,1180TG 5.7% 860.8 / 27.7 288noncoding (72/88 nt)omrAsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (127/142);  new base G (7/12);  total (134/154)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CGCGATACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGT  >  NC_000913/2975969‑2976266
                                                                                                                                                      |                                                                                                                                                     
cgcgaTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGTGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCAt                                                                                                                                                       <  1:3896313/150‑1 (MQ=255)
 gcgaTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATc                                                                                                                                                      >  1:2623289/1‑150 (MQ=255)
 gcgaTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATc                                                                                                                                                      >  2:449411/1‑150 (MQ=255)
 gcgaTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAAACTGCGCATc                                                                                                                                                      >  2:2544195/1‑150 (MQ=255)
   gaTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCcg                                                                                                                                                    >  1:1625406/1‑150 (MQ=255)
    aTACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCcgc                                                                                                                                                   <  2:1294057/150‑1 (MQ=255)
        aaaaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGATTGGTGGGAGATTACACGATATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAg                                                                                                                                               <  1:3270944/150‑1 (MQ=255)
        aaaaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATTGATTGAGATTAAACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGAGAAAAAAAACCTGCGCTTCCGCGCAg                                                                                                                                               >  2:3217953/1‑150 (MQ=255)
         aaaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAgg                                                                                                                                              >  1:3245075/1‑150 (MQ=255)
         aaaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAgg                                                                                                                                              >  2:1089929/1‑150 (MQ=255)
         aaaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAgg                                                                                                                                              <  2:1374867/150‑1 (MQ=255)
         aaaCACGGCACAAATTTCGAAAAAAGATAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAgg                                                                                                                                              <  1:1541117/150‑1 (MQ=255)
          aaCACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGt                                                                                                                                             >  1:1154908/1‑150 (MQ=255)
            ctctgCACAAATTTCTAAAAAAGATAAATATCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCTATTTTAGGTAGATTACACGATATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCGGATGCGCAGGttt                                                                                                                                           <  1:2257182/146‑2 (MQ=255)
            cacgtaacaAATTTCTAAAAAAGCTAAAAAGAATACCAACTCCCTTTGCTCTGATTCAGTAAACGGTATTGGTGGGAGATTACACTAGATAAATACCGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCGGATGCGCAGGttt                                                                                                                                           <  1:4157506/144‑2 (MQ=255)
            cacGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTg                                                                                                                                           <  2:2819034/150‑1 (MQ=255)
             acGGCACACATTTCGAAAAAAGCGAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCAGCCGCGCCGGTTgg                                                                                                                                          >  2:1238941/1‑150 (MQ=255)
              cGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCGGATGCGCAGGttttt                                                                                                                                         <  2:3118360/150‑4 (MQ=255)
              cGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGt                                                                                                                                         <  2:3567578/150‑1 (MQ=255)
              cGGCAAAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCATTAAAAGCGAATGGAGGGTGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTATGTGCGAAAAAAAACCTGCGCGGATGCGCAGGttttt                                                                                                                                         <  2:1379574/150‑4 (MQ=255)
                 caaaaaTTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCa                                                                                                                                      <  1:3011556/147‑1 (MQ=255)
                  acaAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCaa                                                                                                                                     <  2:785258/150‑1 (MQ=255)
                      aTTTCGAAAAAATCTAAAAAGCATTCCAACTCCCTTTGATCTTATTCAGTAAAAGATAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAgaga                                                                                                                                 <  1:3410360/150‑1 (MQ=255)
                       tttCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGAc                                                                                                                                >  1:1385594/1‑150 (MQ=255)
                         tCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCATTAAAAGCGAATGGAGGTAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAg                                                                                                                             <  1:3375374/150‑1 (MQ=255)
                           gAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGAGACAGTAAATTAGGTGCGAAAAAAAAACTGCGCATCCGCGCAGGTTGGTGCAAgagaaacgg                                                                                                                            >  2:2987281/1‑145 (MQ=255)
                             aaaaaGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTa                                                                                                                         >  1:473342/1‑150 (MQ=255)
                              aaaaGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACATGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTAc                                                                                                                        >  2:1502593/1‑150 (MQ=255)
                                   cTAAAAATCATTCCAACTCCCTTTGCTCTGATTCTGTAAAAGCGATTGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAaga                                                                                                                   <  1:831592/150‑1 (MQ=255)
                                    tAAAACGCATTCCAACTCCCTTTGCTCTGATTCATTAAAAGCGAATGGTGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAagag                                                                                                                  <  1:1787759/150‑1 (MQ=255)
                                    tAAAAAGCATTCCAACTCCCTTTGCTCTGATTCTGTAAAAGCGAATGGTGGGTGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAagag                                                                                                                  <  1:844580/150‑1 (MQ=255)
                                    tAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAagag                                                                                                                  >  1:1231254/1‑150 (MQ=255)
                                    tAAAAAGCATTCCAACTCCCTTTGCTATGATTAAGTAAAAGCGAATGGAGGGAGATTACACGATATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAagag                                                                                                                  <  1:2024168/150‑1 (MQ=255)
                                    tAAAAAGAATACCAACTCCCTTTGCTCTTATTCAGTAAAAGCGATTGGTGGTATTTTACACGATATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAagag                                                                                                                  <  1:859848/150‑1 (MQ=255)
                                      aaaaGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCg                                                                                                                >  1:3136661/1‑150 (MQ=255)
                                      aaaaGCATTCCAACTCCCTTTGCTATGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCACGAGACAGGGTACGAAGAGCg                                                                                                                >  1:2271309/1‑150 (MQ=255)
                                        caGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTCACTTAGGTGCGAAAAAAAACCTGCGCATCCGCGCGGGTGGGGGCACGAGACAGGGTACGAAGCGCGtc                                                                                                              >  2:2459828/2‑149 (MQ=255)
                                         aGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTAc                                                                                                             >  1:875093/1‑150 (MQ=255)
                                          gCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTAcc                                                                                                            <  1:3348072/150‑1 (MQ=255)
                                             ttCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGaa                                                                                                         >  1:3721521/1‑150 (MQ=255)
                                               ccAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGTGGTAGATTACACGAGATAAAGACCGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCCTCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGaata                                                                                                       <  1:366357/150‑1 (MQ=255)
                                               ccAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGaata                                                                                                       <  1:4279115/150‑1 (MQ=255)
                                                     cccTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCa                                                                                                 <  2:2777454/150‑1 (MQ=255)
                                                       cTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCAcc                                                                                               <  1:2822555/150‑1 (MQ=255)
                                                       cTTTGCTCTGAGTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCAcc                                                                                               <  2:1911317/150‑1 (MQ=255)
                                                        tttGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACca                                                                                              >  1:952109/1‑150 (MQ=255)
                                                           gCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACcaatc                                                                                           <  1:2347222/150‑1 (MQ=255)
                                                             tctGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACcaatcaa                                                                                         >  1:66773/1‑150 (MQ=255)
                                                             tctGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACcaatcaa                                                                                         >  2:515026/1‑150 (MQ=255)
                                                              ctGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACcaatcaat                                                                                        <  2:566002/150‑1 (MQ=255)
                                                                gATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGGCAATTAGGTGCGAAAAAAAACCTGCGCAGCCGCGCAGGATGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATCATCTCACCCATCAATAc                                                                                      >  2:3910597/1‑150 (MQ=255)
                                                                   tCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACctc                                                                                   >  1:4062980/1‑150 (MQ=255)
                                                                      gTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTgg                                                                                >  1:647427/1‑150 (MQ=255)
                                                                      gTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTgg                                                                                <  2:3861178/150‑1 (MQ=255)
                                                                      gTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATGAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTgg                                                                                <  2:3263253/150‑1 (MQ=255)
                                                                       tAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTggg                                                                               >  1:2532269/1‑150 (MQ=255)
                                                                         aatgCGAATGGAGGGAGATTACACGAGATAAACAACGCGAGCGACAGTATATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTTCCGAATAATCTCACCAATCAATACCTCTGGGAt                                                                             <  2:2719474/147‑1 (MQ=255)
                                                                           aGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCt                                                                           <  2:315697/150‑1 (MQ=255)
                                                                            gCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCtt                                                                          <  1:2954874/150‑1 (MQ=255)
                                                                                aTGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACATGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGAt                                                                       <  2:4055946/150‑1 (MQ=255)
                                                                                   gagggagATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATtgtg                                                                   <  2:1793204/150‑1 (MQ=255)
                                                                                    agggagATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTgg                                                                  <  1:525248/150‑1 (MQ=255)
                                                                                     gggagATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGt                                                                 <  2:1645672/150‑1 (MQ=255)
                                                                                      ggagATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTc                                                                >  2:4233592/1‑150 (MQ=255)
                                                                                        agaTTACACGAGATAACGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTg                                                              <  1:1912324/150‑1 (MQ=255)
                                                                                           ttACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCac                                                           >  2:904959/1‑150 (MQ=255)
                                                                                             acacGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCacga                                                         >  2:1163778/1‑150 (MQ=255)
                                                                                               acGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTgg                                                                  >  2:2304347/1‑139 (MQ=255)
                                                                                                  agaTAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGAct                                                    <  1:265010/150‑1 (MQ=255)
                                                                                                  agaTAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGAct                                                    <  2:3136665/150‑1 (MQ=255)
                                                                                                      aaaGACCGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATCCCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTc                                                <  2:1833901/150‑1 (MQ=255)
                                                                                                        aGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGTATCTTGATTGTGGTCTGCACGACGACTCTTCGc                                              >  2:65029/1‑150 (MQ=255)
                                                                                                           aCGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCAGCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAg                                           <  2:415421/150‑1 (MQ=255)
                                                                                                             gcgagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCg                                         >  2:2656504/1‑150 (MQ=255)
                                                                                                             gcgagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCg                                         >  2:1344466/1‑150 (MQ=255)
                                                                                                               gagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCgag                                       <  2:1736611/150‑1 (MQ=255)
                                                                                                               gagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCgag                                       >  1:1060951/1‑150 (MQ=255)
                                                                                                               gagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCgag                                       >  1:1060946/1‑150 (MQ=255)
                                                                                                               gagcgaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCgag                                       >  2:3790162/1‑150 (MQ=255)
                                                                                                                  taaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGaaa                                    >  2:4031603/3‑150 (MQ=255)
                                                                                                                   gaCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGaaaa                                   <  1:3564862/150‑1 (MQ=255)
                                                                                                                    aCAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAAc                                  <  2:1395216/150‑1 (MQ=255)
                                                                                                                      aGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGc                                >  1:2227332/1‑150 (MQ=255)
                                                                                                                      aGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGc                                >  1:3192169/1‑150 (MQ=255)
                                                                                                                      aGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGc                                >  2:884568/1‑150 (MQ=255)
                                                                                                                           aTTAGGTGCGAAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAg                            >  1:2230479/1‑150 (MQ=255)
                                                                                                                            ttAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGa                          <  2:3820282/150‑1 (MQ=255)
                                                                                                                             tAGGTGCGAAAAAAAACCTGCGCAGCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGaa                         <  2:2043181/150‑1 (MQ=255)
                                                                                                                                 tGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGaa                     <  1:289340/150‑1 (MQ=255)
                                                                                                                                 tGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGaa                     <  1:1413044/150‑1 (MQ=255)
                                                                                                                                 tGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGaa                     <  2:2190674/150‑1 (MQ=255)
                                                                                                                                   cGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAgg                   <  2:1625388/150‑1 (MQ=255)
                                                                                                                                    gAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAggg                  >  2:2603407/1‑150 (MQ=255)
                                                                                                                                           caaCCTGCGCGGATGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGc            >  2:1014634/2‑150 (MQ=255)
                                                                                                                                             aCCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCaa          <  1:2671891/150‑1 (MQ=255)
                                                                                                                                             aCCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCaa          <  1:58982/150‑1 (MQ=255)
                                                                                                                                                   gcATCCGCGCAGGTTGGTGCTAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGgt    <  2:2365117/150‑1 (MQ=255)
                                                                                                                                                   gcATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGgt    <  1:1276915/150‑1 (MQ=255)
                                                                                                                                                    cATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGgtg   >  1:1827612/1‑150 (MQ=255)
                                                                                                                                                    cATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGgtg   >  1:1827594/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGCGATACAAAACACGGCACAAATTTCGAAAAAAGCTAAAAAGCATTCCAACTCCCTTTGCTCTGATTCAGTAAAAGCGAATGGAGGGAGATTACACGAGATAAAGAACGCGAGCGACAGTAAATTAGGTGCGAAAAAAAACCTGCGCATCCGCGCAGGTTGGTGCAAGAGACAGGGTACGAAGAGCGTACCGAATAATCTCACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGT  >  NC_000913/2975969‑2976266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: