Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 619,410 T→C 5.9% T264A (ACG→GCG)  fepC ← ferrienterobactin ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913619,4100TC 5.9% 726.6 / 20.4 220T264A (ACG→GCG) fepCferrienterobactin ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (97/109);  new base C (8/5);  total (106/114)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

GGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAAT  >  NC_000913/619261‑619557
                                                                                                                                                     |                                                                                                                                                   
gggTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGt                                                                                                                                                     <  2:3807412/150‑1 (MQ=255)
gggTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGt                                                                                                                                                     <  1:3481769/150‑1 (MQ=255)
 ggTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTCTTTGCGGTTGAAGGTGCCGta                                                                                                                                                    >  2:2308118/1‑149 (MQ=255)
  gTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGTAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTc                                                                                                                                                   <  2:4114591/150‑1 (MQ=255)
       gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                              >  1:3983972/1‑150 (MQ=255)
       gCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCg                                                                                                                                              >  1:3870070/1‑150 (MQ=255)
         gATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAg                                                                                                                                            <  1:2016000/150‑1 (MQ=255)
             attGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGc                                                                                                                                        <  2:12876/150‑1 (MQ=255)
              ttGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGca                                                                                                                                       <  1:1300236/150‑1 (MQ=255)
              ttGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGca                                                                                                                                       <  1:1687351/150‑1 (MQ=255)
              ttGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGca                                                                                                                                       <  1:2022582/150‑1 (MQ=255)
              ttGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGca                                                                                                                                       <  2:507806/150‑1 (MQ=255)
               tgtgATCCTTTGCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATTATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGGACGAACGGcac                                                                                                                                      <  1:2995377/150‑1 (MQ=255)
               tgtgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTTTGCGATGGCATCCATAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcac                                                                                                                                      <  1:2401869/150‑1 (MQ=255)
                 tgATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGcacca                                                                                                                                    >  1:400118/1‑150 (MQ=255)
                    tCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACaa                                                                                                                                 >  2:1352710/1‑150 (MQ=255)
                        ttCCGAGTTGATATGCGGTTTGGTGGCTTGTGGTGGGGTGCTGTTGCGCTATGCTATGGCATCAAGAAAACAGTTTGCCATGATGGCAGAACAATTAGTTTATGTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgctg                                                                                                                             <  2:3677893/150‑3 (MQ=255)
                         tCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCTCTATGCGATGGCATCAAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgcgtc                                                                                                                            <  2:4063259/150‑3 (MQ=255)
                         tCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAgcggc                                                                                                                            <  2:3720137/150‑1 (MQ=255)
                           cgcgTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGt                                                                                                                          <  2:1491785/150‑1 (MQ=255)
                              gTTGATCGGCGGGTTGGTGGCTTGTGGTGGCGTGCTGTTGCGATATGCGATGGCATCCATAAAACAGGATGCCATGATGGCAGACAACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTcc                                                                                                                       <  1:4007105/150‑1 (MQ=255)
                               ttGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCg                                                                                                                      >  1:549001/1‑150 (MQ=255)
                                tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGTCATCCAGAAAACAGGATGCAATGATGGCAGACCACTTAGTTTAACTTTTTGCGGTTGAAGGTGCCGTTCGTCCTAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                     <  1:2941246/150‑1 (MQ=255)
                                tGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTTGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCgg                                                                                                                     <  1:1757224/150‑1 (MQ=255)
                                   tCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCaa                                                                                                                  <  2:224977/150‑1 (MQ=255)
                                   tCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCaa                                                                                                                  <  2:2014619/150‑1 (MQ=255)
                                        gggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGa                                                                                                             <  1:498955/150‑1 (MQ=255)
                                         ggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACCGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGAt                                                                                                            <  1:2797854/150‑1 (MQ=255)
                                         ggATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGAt                                                                                                            <  1:487601/150‑1 (MQ=255)
                                          gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                           <  1:3408026/150‑1 (MQ=255)
                                          gATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATc                                                                                                           <  2:2740724/150‑1 (MQ=255)
                                            tggtggCTTGTGGTGGCGTGCTGTTGCGCTTTGCGATGGCATCCAGAAAACAGGATGCATTGTTGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGt                                                                                                         <  2:2509602/150‑1 (MQ=255)
                                            tggtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGt                                                                                                         <  2:20585/150‑1 (MQ=255)
                                              gtggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCa                                                                                                       >  2:552528/1‑150 (MQ=255)
                                               tggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGTATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                      <  1:3029994/150‑1 (MQ=255)
                                               tggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                      <  2:2298434/150‑1 (MQ=255)
                                               tggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCaa                                                                                                      <  1:881700/150‑1 (MQ=255)
                                                ggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAAt                                                                                                     <  2:2455877/150‑1 (MQ=255)
                                                ggCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAAt                                                                                                     <  2:3326411/150‑1 (MQ=255)
                                                   ttGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGAt                                                                                                  >  1:1990095/1‑150 (MQ=255)
                                                    tgtgGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATc                                                                                                 <  1:1071811/150‑1 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCAt                                                                                               >  1:606658/1‑150 (MQ=255)
                                                      tggtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGCTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATTATCAt                                                                                               >  1:115583/1‑150 (MQ=255)
                                                        gtggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGc                                                                                             >  1:327401/1‑150 (MQ=255)
                                                          ggCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAg                                                                                           >  1:804915/1‑150 (MQ=255)
                                                            cGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAgcg                                                                                         >  2:510968/1‑150 (MQ=255)
                                                                 tGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGAc                                                                                    <  1:3290207/150‑1 (MQ=255)
                                                                 tGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGAc                                                                                    <  2:1967601/150‑1 (MQ=255)
                                                                   ttGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCa                                                                                  >  2:6853/1‑150 (MQ=255)
                                                                     gcgcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATa                                                                                <  1:840213/150‑1 (MQ=255)
                                                                      cgcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATaa                                                                               <  1:238141/150‑1 (MQ=255)
                                                                       gcTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATaaa                                                                              >  2:4198018/1‑150 (MQ=255)
                                                                        cTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAAt                                                                             <  2:1846548/150‑1 (MQ=255)
                                                                         tATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATg                                                                            <  1:3347905/150‑1 (MQ=255)
                                                                         tATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATg                                                                            >  1:656287/1‑150 (MQ=255)
                                                                         tATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATg                                                                            <  1:3176392/150‑1 (MQ=255)
                                                                           tGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATCAATgcg                                                                          >  1:2491357/1‑150 (MQ=255)
                                                                            gCGATGGCATCCTGAAAACAGGATGCCTTGTTGGCAGACCTCTTAGTTTATCTATTTGCGGTTGAAGGTGCCGTTCGTCCGCGCGGGACCTGAAGGGGCGTTGCGGCTACCGGATCGTGTATGTTCATGCTGCGCAGTCCTTAAATgcgg                                                                         <  2:1782909/150‑2 (MQ=255)
                                                                            gCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcgc                                                                         <  2:1525945/150‑1 (MQ=255)
                                                                            gCGAGGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGCGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATgcgc                                                                         <  2:1394692/150‑1 (MQ=255)
                                                                                tGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCaa                                                                     >  1:2787310/1‑150 (MQ=255)
                                                                                tGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCaa                                                                     <  2:190282/150‑1 (MQ=255)
                                                                                   cttCCATAAAACTGTTTGCATTGATGGCTGACCATTTTGTTTAAGTTTTTGGGGTTGATGGTGCGGCTGGTCCGAGGGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCa                                                                  <  2:3450770/148‑1 (MQ=255)
                                                                                    aTCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAg                                                                 >  2:3005303/1‑150 (MQ=255)
                                                                                    aTCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAg                                                                 <  1:3116498/150‑1 (MQ=255)
                                                                                    aTCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAg                                                                 >  2:856391/1‑150 (MQ=255)
                                                                                        aGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCCTAAATGCGCTCAATCAGTTca                                                             >  1:377153/1‑150 (MQ=255)
                                                                                        aGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTca                                                             <  2:3870070/150‑1 (MQ=255)
                                                                                           aaaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagca                                                          >  1:2786219/1‑150 (MQ=255)
                                                                                            aaCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTcagcag                                                         >  1:2630371/1‑150 (MQ=255)
                                                                                             aCAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGt                                                        <  2:607945/150‑1 (MQ=255)
                                                                                                  aTGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACaa                                                   <  2:404989/150‑1 (MQ=255)
                                                                                                   tGCCATGATGGCAGACCACTTAGTTTAACTATGTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAt                                                  <  2:2188592/150‑1 (MQ=255)
                                                                                                      cATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAAtctc                                               >  1:2075913/1‑150 (MQ=255)
                                                                                                       atgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTcc                                              >  2:3614378/1‑150 (MQ=255)
                                                                                                        tgatgGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCACTGAGCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCt                                             >  2:2694248/1‑150 (MQ=255)
                                                                                                                gACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCt                                     >  2:507140/1‑150 (MQ=255)
                                                                                                                gACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCt                                     >  2:2371898/1‑150 (MQ=255)
                                                                                                                      ttAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                               >  1:3933076/1‑150 (MQ=255)
                                                                                                                      ttAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                               <  2:2174811/150‑1 (MQ=255)
                                                                                                                      ttAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                               >  1:1803441/1‑150 (MQ=255)
                                                                                                                      ttAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                               <  1:1420956/150‑1 (MQ=255)
                                                                                                                      ttAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGa                               <  1:1779629/150‑1 (MQ=255)
                                                                                                                       tAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAg                              <  1:1313200/150‑1 (MQ=255)
                                                                                                                       tAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAg                              <  1:4207037/150‑1 (MQ=255)
                                                                                                                          tttAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGcaa                           >  2:307781/1‑150 (MQ=255)
                                                                                                                                tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                     >  2:3786883/1‑150 (MQ=255)
                                                                                                                                tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                     >  1:1614189/1‑150 (MQ=255)
                                                                                                                                tATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAttt                     >  1:1525963/1‑150 (MQ=255)
                                                                                                                                 aTTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAAtttt                    <  1:1564164/150‑1 (MQ=255)
                                                                                                                                  tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttc                   <  2:3983972/150‑1 (MQ=255)
                                                                                                                                  tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttc                   >  2:3100601/1‑150 (MQ=255)
                                                                                                                                  tttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttc                   <  1:2654948/150‑1 (MQ=255)
                                                                                                                                   ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGTCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                  <  1:2415508/150‑1 (MQ=255)
                                                                                                                                   ttGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcc                  <  1:1835771/150‑1 (MQ=255)
                                                                                                                                       ggTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttc              <  2:2923415/150‑1 (MQ=255)
                                                                                                                                        gTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttcc             >  2:2969501/1‑150 (MQ=255)
                                                                                                                                         ttGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTttcccttccc            >  1:2577597/1‑150 (MQ=255)
                                                                                                                                              ggTGCCGCTCGGACGAACGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaat       >  2:320281/1‑150 (MQ=255)
                                                                                                                                                  ccGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCgcaatgcaa   <  1:1208129/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGGTAAGGCGATTATTGTGATCCTTTCCGCGTTGATCGGCGGGATGGTGGCTTGTGGTGGCGTGCTGTTGCGCTATGCGATGGCATCCAGAAAACAGGATGCCATGATGGCAGACCACTTAGTTTAACTATTTGCGGTTGAAGGTGCCGTTCGTCCGAGCGGCACCACAAGCGGCGTTCCGGCAACTGGATCGTCAATGATCATGCAGCGCAGACCATAAATGCGCTCAATCAGTTCAGCAGTGACAATCTCCTTCGGCGCTCCCTGAGCAACAATTTTCCCTTCCCGCAATGCAAT  >  NC_000913/619261‑619557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: