Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 7.1% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 7.1% 521.0 / 23.0 199intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (120/61);  new base T (7/7);  total (130/69)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 205 total aligned reads displayed.

ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3269997‑3270292
                                                                                                                                                  |                                                                                                                                                     
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                  <  2:2788218/150‑1 (MQ=37)
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                  <  1:1233684/150‑1 (MQ=37)
   gacgtaTAAGCCGCTTTCTTTTGTTTATTGTGTGTTGGACATTCATTCATCTTTCTTCCCCTCAAAAATCTATAACCCGCCGAAGCGGGTTTTTTCGTAAACGATGTTATACTGAACGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                                                                                 <  1:1090806/145‑1 (MQ=11)
    aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGGAAAGCGTAGTgg                                                                                                                                                >  2:353725/1‑150 (MQ=255)
        aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                                                                                                                            <  2:1453043/150‑1 (MQ=37)
           aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtca                                                                                                                                         <  1:337056/150‑1 (MQ=37)
            gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGtcat                                                                                                                                        >  2:96006/1‑150 (MQ=37)
             ccGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATCTTTATTCCCCACCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAACCCGGTGAAAAAGACCGAAAATCCGCGTTAGTTTGGGGATAGCGTCGTGGACAGtcatt                                                                                                                                       >  1:3209401/1‑150 (MQ=11)
                        tgtgtttaGTGTCGTCGACAGACTCTCTACTTTCTGCCACTCCAAAATCAAAAACCCGCCGTAGCGGGTTTTTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGc                                                                                                                            <  1:2833695/144‑1 (MQ=11)
                            tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCt                                                                                                                        <  1:1498448/150‑1 (MQ=37)
                                  gtCGTGGAAAGTCTTTCATCTTTATGCCACTCCAAAATCAAAAACCCGCCGCAGCGGGTTTTTACGTAAAGCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                  <  2:3057514/150‑1 (MQ=11)
                                   tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCAGTGGGGTAGAGCGTCGGGGCAAGTCCTTCAACGTTCAGCCCCGACaaaac                                                                                                                 >  2:370388/1‑149 (MQ=11)
                                      tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCCAAAGCaa                                                                                                              >  1:1607691/1‑150 (MQ=255)
                                       ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCATTCTTCACCTACAAAAGCaaa                                                                                                             >  2:2299303/1‑150 (MQ=11)
                                        gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaa                                                                                                            >  1:3262025/1‑150 (MQ=37)
                                        gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCaaaa                                                                                                            >  2:1511613/1‑150 (MQ=37)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTc                                                                                                                               <  2:3316340/128‑1 (MQ=38)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                         >  1:3209935/1‑150 (MQ=37)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAAcc                                                                                                         >  1:402660/1‑150 (MQ=37)
                                              cattcttATTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAACAGCAAAAACccgc                                                                                                      <  2:4182289/150‑1 (MQ=11)
                                              cattctaCTTTCTGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                                                                      <  2:2157117/150‑1 (MQ=255)
                                                ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                    >  1:1763734/1‑150 (MQ=37)
                                                ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                    >  1:217326/1‑150 (MQ=37)
                                                ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGGGGGGGCTAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCGCCAAAAGCAAAAACccgccg                                                                                                    >  2:1121656/1‑150 (MQ=11)
                                                 tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATGTTTCTGCCCCTCCAAAAGCAAAAAAccgccta                                                                                                   >  2:1731672/1‑148 (MQ=255)
                                                  catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCt                                                                                                                              <  1:568233/122‑1 (MQ=38)
                                                  catCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGACGAGGACAGTCATTCATCTTTCGGCCCCACCAAAAGCCAAAACCCGCCGaa                                                                                                  >  2:640689/1‑150 (MQ=11)
                                                     cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTGTCTGTGGGGGATAGCGTCGGGGCCAGGCCTGCATCGTTCTGCCCCACCAAAAGCACAAACCCGCCGCAGCg                                                                                               >  2:2961517/1‑150 (MQ=11)
                                                      tttCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              <  2:1781429/150‑1 (MQ=12)
                                                          tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                          >  2:2407593/1‑150 (MQ=37)
                                                           gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                         >  1:3822886/1‑150 (MQ=37)
                                                            ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCTTCATCTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                        >  2:580760/1‑150 (MQ=255)
                                                            ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                        >  1:183163/1‑150 (MQ=37)
                                                            ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttgt                                                                                        >  1:2915113/1‑148 (MQ=37)
                                                            ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGgtttt                                                                                        >  1:2864258/1‑150 (MQ=37)
                                                            ccccACCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                        <  1:4050561/150‑1 (MQ=11)
                                                             cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                               <  1:713025/94‑1 (MQ=40)
                                                             cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                       >  2:3676681/1‑150 (MQ=37)
                                                             cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                               >  2:713025/1‑94 (MQ=18)
                                                              ccTCCAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGTTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      <  1:1819842/150‑1 (MQ=11)
                                                              ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                      >  1:3532475/1‑150 (MQ=37)
                                                               cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                                                                     >  1:3351275/1‑150 (MQ=37)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                    >  1:3392864/1‑150 (MQ=37)
                                                                   aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTaaa                                                                                 >  2:2116737/1‑150 (MQ=37)
                                                                       gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTTCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                             >  2:2848600/1‑150 (MQ=25)
                                                                        cAAAAACCCTCCGAAGCGGGTTATTACGAAAACCTGGTGAATCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                            <  2:3906264/150‑1 (MQ=11)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATAATTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAATCAgg                                                                            >  2:3132730/1‑150 (MQ=11)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTAGAGCGTCGTGGACAGTCATTCATCTTTAGGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTAATACGGAAATCAgg                                                                            >  2:2859834/1‑150 (MQ=11)
                                                                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                           >  1:2555963/1‑150 (MQ=37)
                                                                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                           >  1:3906455/1‑150 (MQ=37)
                                                                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGt                                                                           >  2:2696880/1‑150 (MQ=37)
                                                                         aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGGGTTACGTAAATCAGGt                                                                           >  2:1540873/1‑150 (MQ=255)
                                                                           aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                                                                         >  1:998032/1‑150 (MQ=37)
                                                                           aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                                                                         >  2:212460/1‑150 (MQ=37)
                                                                             aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                       <  1:2592218/150‑1 (MQ=37)
                                                                                 gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATATCGTCGTGGAAAGTAAATAATCTTTCTGCCCCACCAAAAGCAAAAAACCGACCAAGAGGGTTTTTAAGTAAATCAGGTGAAACTGa                                                                   >  2:456293/1‑150 (MQ=11)
                                                                                 gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGTGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                   >  1:3417315/1‑150 (MQ=37)
                                                                                  ccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGTGAAACTGAc                                                                  >  2:3137240/1‑150 (MQ=37)
                                                                                     aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGTCAGTCAACGTTCTGCCCCTCCAAAAGCAAAATCCCGCCGACGCGGGTGTTTACGTAAAGCAGGTGAAACGGACCgc                                                               >  2:1268929/1‑149 (MQ=11)
                                                                                        cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                            >  2:101779/1‑150 (MQ=37)
                                                                                           gTTTTTACGTACATCAGGTTACACTGACCGATAAGCCGCTTTCTTTTGGGTATTGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                         <  2:2748813/150‑1 (MQ=11)
                                                                                              tttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCggg                                                      <  1:4258939/150‑1 (MQ=18)
                                                                                               ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt                                                     >  2:2914586/1‑150 (MQ=39)
                                                                                                tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt                                                    >  1:2418940/1‑150 (MQ=40)
                                                                                                   gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTACATCAGGTGAAACTGACCGATAATCCGGGTTCTg                                                 >  1:2284704/1‑150 (MQ=255)
                                                                                                    tAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                                >  2:2307611/1‑150 (MQ=255)
                                                                                                     acatcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  1:935821/146‑1 (MQ=255)
                                                                                                     aaaaCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  2:1682402/150‑1 (MQ=255)
                                                                                                        ccAGTTGAAACTGTCCGTTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg                                            <  1:1742975/150‑1 (MQ=255)
                                                                                                         cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAACCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                           >  1:2001055/1‑150 (MQ=255)
                                                                                                          aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTAGTggt                                          >  1:638599/1‑149 (MQ=255)
                                                                                                             taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTgtggtgtaaag                                       >  2:2994514/3‑145 (MQ=11)
                                                                                                             tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGGCATTCATATTTCTGCCCCGCCAAAAGCAAAAACCCCCCCCAGCGGGGTTTTTAGTAAAACAGGTGGAACAGACCGATAAGCCCGGTTCTGTCGGGGGCAg                                       >  1:851939/1‑150 (MQ=14)
                                                                                                                aaatGACCGATTAGACGCTTTCTTTTTGTTTTAGTGTAGTGTACAGTCTTTCATCTTTATGCCCCTCCAAAAGAAATAACCCTCCTAAGCGTTTTTTTACGTAAATCAGGTGAAACTGACAGATATGCAGGGTTCTGTCGTGTACAGtca                                    <  2:845457/147‑1 (MQ=255)
                                                                                                                     aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGAACGATAAGCCGGGTTCTGTCGTGGACAGtcattaat                               >  2:2196308/1‑150 (MQ=14)
                                                                                                                      ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                              <  1:3747769/150‑1 (MQ=255)
                                                                                                                      ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                              <  1:1927255/150‑1 (MQ=255)
                                                                                                                        gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  2:2353387/150‑1 (MQ=255)
                                                                                                                        gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                            <  1:2165100/150‑1 (MQ=255)
                                                                                                                        gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGACCGAAAAGCCGGGTTCTGTCGTGGACAGTCATTCAtata                            >  2:1665305/1‑150 (MQ=255)
                                                                                                                        gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCCTTCATCTa                            >  2:3729153/1‑150 (MQ=17)
                                                                                                                         cgcaGCCGCTATCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                           <  2:4073312/147‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  1:1511681/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  2:3209935/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAACAAGGCCa                       >  2:2623316/1‑150 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAATCAAAAACCCCCCGAAGCGGGTTTTTACGTAAATAAGGTGAAAAAGACCGATAAGCCGGGGTCTTTAGTGGACAGTCATTCATAGAGGGCAg                      >  1:3128583/1‑150 (MQ=14)
                                                                                                                                gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTAGTGGACAGTCAATCATCTAGTCCATCa                    >  2:806890/1‑150 (MQ=11)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAAATGCCCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAACTAGGCCCGCa                    >  1:1913016/1‑150 (MQ=17)
                                                                                                                                  tttctttTGTTTATAGCGACGTGGCCATTCTTTCATGATTCTGCTGCACCCAGATCATAATGGCGCCGTAGCGTTTTATTACGTACATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                  <  1:2463605/150‑1 (MQ=16)
                                                                                                                                  tttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                  <  2:3747846/150‑1 (MQ=255)
                                                                                                                                  tttctttTGAGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAATTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCCTTCATCTAGGCCAGCaaa                  >  1:3077766/1‑149 (MQ=255)
                                                                                                                                   ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                 <  1:806893/150‑1 (MQ=255)
                                                                                                                                       tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGGTCTGTCGTGGTCAGTCATTCATCTAGGCCCGCACTCGCTc             >  1:3668557/1‑150 (MQ=18)
                                                                                                                                          gggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt          <  1:2407593/150‑1 (MQ=255)
                                                                                                                                          gggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACt          >  2:2268943/1‑150 (MQ=255)
                                                                                                                                          gggTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCGCCCAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGGTCTGTGGTGGACAGTCATTCATCTAGGCCAGCAAACGCTCAca          >  1:798918/1‑149 (MQ=18)
                                                                                                                                            gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg        >  2:2025879/1‑150 (MQ=255)
                                                                                                                                            gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg        >  2:3347130/1‑150 (MQ=255)
                                                                                                                                               taGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACCGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc     <  1:2961517/150‑1 (MQ=255)
                                                                                                                                                  cgtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGCACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAag  >  2:23307/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3269997‑3270292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: