Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,220 C→G 5.8% L132V (CTG→GTG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2200CG 5.8% 670.9 / 27.9 190L132V (CTG→GTG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base C (90/89);  new base G (7/4);  total (97/93)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-01

Only 100 of 191 total aligned reads displayed.

TCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTT  >  NC_000913/3368071‑3368368
                                                                                                                                                     |                                                                                                                                                    
  tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                                                                    <  1:477189/150‑1 (MQ=255)
  tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACATCTTTTTTCAACATTTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCTTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                                                                    <  1:1229684/150‑1 (MQ=255)
    tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCGCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                  <  1:1843757/150‑1 (MQ=255)
      cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                                                                                >  1:3886595/1‑150 (MQ=255)
      cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                                                                                >  2:464534/1‑150 (MQ=255)
       aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                               >  2:2677838/1‑150 (MQ=255)
        aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                                              >  1:2244119/1‑150 (MQ=255)
        aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGcc                                                                                                                                              <  2:2547658/150‑1 (MQ=255)
        aaaaaCGTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGcc                                                                                                                                              <  2:2547653/150‑1 (MQ=255)
           aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaaa                                                                                                                                           <  2:1881314/150‑1 (MQ=255)
                  gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                                                                    >  2:3214823/1‑150 (MQ=255)
                      attGAGGCTTTTTTACCTCTTTTTTCAACTTGTTGAAATTCACCAGACATTGTGTGGCGAATTAACACCATTGGTAATCCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCtt                                                                                                                                <  1:2127266/148‑1 (MQ=255)
                        tGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGc                                                                                                                              <  2:2787349/150‑1 (MQ=255)
                            gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCCGACAATGTCGGGCGAATAAACACCATGGGTAATTCCGCAGACACCTTCTGAAGAATTCCCATCGGCAAAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                                                          <  1:3945775/150‑1 (MQ=255)
                             cTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTGTCCCCTTGCCCAgg                                                                                                                         >  1:629994/1‑150 (MQ=255)
                              ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATGGGGAATTCCGCAGACACCGTCTGCAGAATGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                                                        <  1:581413/150‑1 (MQ=255)
                               tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                                                       >  1:1056013/1‑150 (MQ=255)
                                ttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                                                                      <  2:4115596/150‑1 (MQ=255)
                                       tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACg                                                                                                               >  1:1598818/1‑150 (MQ=255)
                                       tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                               >  1:278307/1‑150 (MQ=255)
                                       tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                               >  2:3198702/1‑150 (MQ=255)
                                        ctTTTTTCAACATGTTGAAATTCACCATACAAAGTCTGGCGAAAAAACACCATAGGGAATTCCGCAGACACCCTCAGCAGAATACCCATCCGCAAAGATTTGGCAGAAGCTCTCAGCCAAATCTAACCCCAAGCCCAGGACCACCTACgc                                                                                                              >  1:3996876/1‑149 (MQ=255)
                                        ctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGt                                                                                                              >  1:3065357/1‑150 (MQ=255)
                                             ttCAACCTGTGGAAATTCACCAGACACTGTCTGGCGAATTAACACCTTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGtt                                                                                                         <  1:1259386/150‑1 (MQ=255)
                                              tCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTGTGCCCAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGttt                                                                                                        >  1:3042369/1‑150 (MQ=255)
                                               cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                                                                       <  1:250965/150‑1 (MQ=255)
                                               cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTa                                                                                                       >  1:358063/1‑150 (MQ=255)
                                                     gTTGAAATTCACCATACAATGTCTGGCGAATTACCACCATTGGGACTTCCGCAGACACCGTCTGCAGAATTCCAATCGGCAAAGTTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                 <  1:1355913/150‑1 (MQ=255)
                                                     gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                 <  2:1317489/150‑1 (MQ=255)
                                                     gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                 <  2:1317450/150‑1 (MQ=255)
                                                     gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                 <  2:2343618/150‑1 (MQ=255)
                                                        gAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGtt                                                                                              >  1:2823863/1‑150 (MQ=255)
                                                           aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                           <  1:836689/150‑1 (MQ=255)
                                                           aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                           <  1:2862841/150‑1 (MQ=255)
                                                           aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTGTCCGTTaaa                                                                                           >  2:618499/1‑150 (MQ=255)
                                                             tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaa                                                                                         >  2:563879/1‑150 (MQ=255)
                                                               gccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                       >  2:809313/2‑150 (MQ=255)
                                                               gccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                       >  2:2555333/2‑150 (MQ=255)
                                                               aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                       <  2:2896118/150‑1 (MQ=255)
                                                                ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGTAGCTGTCTGCCAAATCTTTCGCGTTGCCCAGGTCCACCTTAGTGCGTTTACTTTGCGTTAAAATCgg                                                                                      >  2:3283672/1‑149 (MQ=255)
                                                                ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                      >  2:2515850/1‑150 (MQ=255)
                                                                 cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                                     <  2:3640096/150‑1 (MQ=255)
                                                                 cAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCt                                                                                     <  2:2481553/150‑1 (MQ=255)
                                                                   gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                                                   <  2:4155176/150‑1 (MQ=255)
                                                                   gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCAACGGCCGTTTACTTTCCGTTAAAAACGCTGa                                                                                   >  1:1996726/1‑150 (MQ=255)
                                                                        tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                              >  2:2006999/1‑150 (MQ=255)
                                                                         gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCAGTCTGCCAAATCATTACCCTTGCCCAGGTCCCCCTACCGCCGTGTACTTTCCGGTAAAAACGCTGACGCCCg                                                                             >  1:1437355/1‑150 (MQ=255)
                                                                            tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAg                                                                          >  2:1066992/1‑150 (MQ=255)
                                                                            tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAAGCTGTCAGCCAAATCTTTCCCCTTGCCCAGGTCCACCAACGTCCGTTTACTTTCCGTTAAAAACGCTGTCGCCCGccg                                                                          >  1:3569053/1‑148 (MQ=255)
                                                                              gCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGGCGCTGGGTGCCAAATCTTTGCCCTTGCCCCGGGCCACCAACGTCCGTTTACTTTGCGTTAAAAAAGCGGGCGCCCGccggt                                                                        >  1:3608141/1‑146 (MQ=255)
                                                                                  aTTAACACCATTGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                                                    <  2:495808/150‑1 (MQ=255)
                                                                                   ttAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTAt                                                                   <  1:822234/150‑1 (MQ=255)
                                                                                       cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                               <  2:314489/150‑1 (MQ=255)
                                                                                        acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaa                                                              <  2:3081923/150‑1 (MQ=255)
                                                                                        acCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAAAATTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTGAAAAAAGCGGCCGCCCGCAGCTTTTATGaaaa                                                              >  1:3731544/1‑150 (MQ=255)
                                                                                         ccATTGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                                                             <  2:3149657/150‑1 (MQ=255)
                                                                                              gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc                                                        <  1:917749/150‑1 (MQ=255)
                                                                                              gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc                                                        <  2:1522367/150‑1 (MQ=255)
                                                                                                  aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTc                                                    <  2:2048912/150‑1 (MQ=255)
                                                                                                   ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCTACGTCCGGTTACATTCCGGTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTcc                                                   >  1:2868967/1‑150 (MQ=255)
                                                                                                   ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGGCTGCCAAATCTTTACCCATGCCCAGGTCCACCTACGTCCGGTTACTTTCCGGTAAAAACGCGGACGCCCCCCGCTTTTTTGAAAAAGTGAAGCTcc                                                   >  1:3100269/1‑150 (MQ=255)
                                                                                                      cGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGCGACGCTCCGCt                                                >  2:3629781/1‑150 (MQ=255)
                                                                                                       gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                               <  1:2677838/150‑1 (MQ=255)
                                                                                                       gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                               <  2:4003793/150‑1 (MQ=255)
                                                                                                       gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGCAGCGTTTATGAAAAAGAGACGcgccgcgg                                               >  1:255849/1‑148 (MQ=255)
                                                                                                         aGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTGCGTTTTCTTTGGGTTAAAAAGGCTGTCGCCCGCTGGTTTTTTGTTTAAGAGTGGCTCCGCtgtg                                             >  2:1949674/1‑150 (MQ=255)
                                                                                                         aGACACCGTCTGCAGAAGGCCCATCGGCCCAGCTTGGGCAGCCGCTGTCTGCCAAATCTTTCCCCTTGCCCCGGTCCACCTACGTCCGTTTACTGTCCCTTACAAACGCTGACGCCCGCAGCGTTTATGAAAAAGAGACGCTCCGCtgtg                                             <  1:865819/150‑1 (MQ=255)
                                                                                                          gACACCGTCTGCAGAATGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                            <  2:3906075/150‑1 (MQ=255)
                                                                                                           acacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGc                                           <  2:3325687/150‑1 (MQ=255)
                                                                                                            cacCGTCTGCAGAATTCCCATCGGCAAAGATTGGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCt                                          <  2:3851958/150‑1 (MQ=255)
                                                                                                             acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg                                         <  1:2724215/150‑1 (MQ=255)
                                                                                                               cGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                                       <  1:2785311/150‑1 (MQ=255)
                                                                                                                   tGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt                                   <  1:2011667/150‑1 (MQ=255)
                                                                                                                    gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                                  <  1:2165665/150‑1 (MQ=255)
                                                                                                                    gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                                  <  1:3376262/150‑1 (MQ=255)
                                                                                                                         aTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCATATCTTTCCCCTTGCCCAGGTCCTCCTACGTCCGTTTACTTTCCGTTAAATACGCTGACGCCCGCAGCTTTTTTGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                             <  1:3651926/150‑1 (MQ=255)
                                                                                                                         aTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                             <  1:3214705/150‑1 (MQ=255)
                                                                                                                             ccATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCGTTTAGGGAAAAGAGACGCTCCGCTGTGGCTGGTCGGTTCGCCAGCTa                         >  2:3151185/1‑150 (MQ=255)
                                                                                                                              cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                        >  2:3351461/1‑150 (MQ=255)
                                                                                                                              cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                        >  2:104399/1‑150 (MQ=255)
                                                                                                                              cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                        >  2:2089895/1‑150 (MQ=255)
                                                                                                                                    cAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGc                  >  2:3158424/1‑150 (MQ=255)
                                                                                                                                     aaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAACGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCa                 >  2:3334482/1‑150 (MQ=255)
                                                                                                                                     aaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCGGTGGCTGGTCTGGTCGCCAGCTAGAGCGGCa                 >  2:2017899/1‑150 (MQ=255)
                                                                                                                                      aaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCaa                >  2:2987730/1‑150 (MQ=255)
                                                                                                                                        caTTTGGCAGACGCTGTCTCCCAAATCTTTCCCCTTGCCCACGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCCTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCaaac              <  1:2936425/149‑2 (MQ=255)
                                                                                                                                         ctttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt             <  1:281625/149‑1 (MQ=255)
                                                                                                                                         aTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAGAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt             <  1:2760132/150‑1 (MQ=255)
                                                                                                                                         aTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt             >  2:2879806/1‑150 (MQ=255)
                                                                                                                                          tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg            >  2:4266671/1‑150 (MQ=255)
                                                                                                                                          tttGGCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg            >  1:2498856/1‑150 (MQ=255)
                                                                                                                                          ctcgccAGACGCTGACCGCCAAAACTGACCCCTCGCCCCGGTCCACCGACGTCCGATCACCTTCCCTCAAACACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAATg            <  2:2174486/145‑1 (MQ=255)
                                                                                                                                            tGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg          <  1:4092501/150‑1 (MQ=255)
                                                                                                                                            tGGCAGACAGCGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg          >  2:1722937/1‑150 (MQ=255)
                                                                                                                                            tGGCAGACAGCGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg          >  2:3857091/1‑150 (MQ=255)
                                                                                                                                             ggCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTCTGACAACGAGACGCTCCGCGGTGGCTGGGCGGTTCGCCAGCTAGAGCGGCACATTGCgc         >  2:2682749/1‑149 (MQ=255)
                                                                                                                                               cAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAcc       >  1:1006671/1‑150 (MQ=255)
                                                                                                                                               cAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAcc       >  1:1006668/1‑150 (MQ=255)
                                                                                                                                                aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc      >  2:3470117/1‑150 (MQ=255)
                                                                                                                                                  aCGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAg    >  2:305741/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTT  >  NC_000913/3368071‑3368368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: