Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,368,221 T→C 5.8% L132P (CTG→CCG) ‡ yhcG → DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,368,2210TC 5.8% 589.4 / 19.5 190L132P (CTG→CCG) ‡yhcGDUF1016 family protein in the PD‑(D/E)XK nuclease superfamily
Reads supporting (aligned to +/- strand):  ref base T (86/89);  new base C (7/4);  total (97/93)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-02

Only 100 of 193 total aligned reads displayed.

TCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTT  >  NC_000913/3368073‑3368370
                                                                                                                                                    |                                                                                                                                                     
tctGCAAAAAACTTACGCCAAATGAGGCTTTTTTACATCTTTTTTCAACATTTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCTTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTg                                                                                                                                                      <  1:1229684/150‑1 (MQ=255)
  tGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCGCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTc                                                                                                                                                    <  1:1843757/150‑1 (MQ=255)
   gCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGGCTGCAGACTTCCCATCGGCAAAGATTTGGCAGACGCTGTCt                                                                                                                                                   >  1:3633262/1‑150 (MQ=255)
    cAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTg                                                                                                                                                  >  2:464534/1‑150 (MQ=255)
     aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                                 >  1:4003793/1‑150 (MQ=255)
     aaaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGc                                                                                                                                                 >  2:2677838/1‑150 (MQ=255)
      aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGcc                                                                                                                                                >  2:3091626/1‑150 (MQ=255)
      aaaaaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGcc                                                                                                                                                <  2:2547657/150‑1 (MQ=255)
         aaCTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACAGCGTCTGCCaaa                                                                                                                                             <  2:3662003/150‑1 (MQ=255)
                gCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcc                                                                                                                                      >  2:3214823/1‑150 (MQ=255)
                    attGAGGCTTTTTTACCTCTTTTTTCAACTTGTTGAAATTCACCAGACATTGTGTGGCGAATTAACACCATTGGTAATCCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAATATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCtt                                                                                                                                  <  1:2127266/148‑1 (MQ=255)
                      tGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGc                                                                                                                                <  2:2787349/150‑1 (MQ=255)
                          gattttttACCTCTTTTTTCAACATGTTGAAATACACCCGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCATAATTCCCAGCGGCAACGTTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                                                            <  1:3945715/148‑1 (MQ=255)
                          gCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAg                                                                                                                            >  1:2457802/1‑150 (MQ=255)
                            ttttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATGGGGAATTCCGCAGACACCGTCTGCAGAATGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGt                                                                                                                          <  1:581413/150‑1 (MQ=255)
                             tttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTc                                                                                                                         >  1:1056013/1‑150 (MQ=255)
                              ttttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTcc                                                                                                                        <  2:4115596/150‑1 (MQ=255)
                               tttACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCCTCTGCAGAATTCCCATCGGCAAAGATTTTGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCa                                                                                                                       >  1:3954973/1‑150 (MQ=255)
                                  aCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCt                                                                                                                    <  1:282970/150‑1 (MQ=255)
                                     tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACg                                                                                                                 >  1:1598818/1‑150 (MQ=255)
                                     tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                                 >  2:3198702/1‑150 (MQ=255)
                                     tctTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACg                                                                                                                 >  1:278307/1‑150 (MQ=255)
                                           ttCAACCTGTGGAAATTCACCAGACACTGTCTGGCGAATTAACACCTTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGtt                                                                                                           <  1:1259386/150‑1 (MQ=255)
                                             cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCCCCTACGTCCGTTTa                                                                                                         <  1:250955/150‑1 (MQ=255)
                                             cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTa                                                                                                         <  1:250965/150‑1 (MQ=255)
                                             cAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCGTGCCCAGGTCCACCTACGTCCGTTTa                                                                                                         >  1:358063/1‑150 (MQ=255)
                                              aaCATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTAc                                                                                                        >  1:3046384/1‑150 (MQ=255)
                                                   gTTGAAATTCACCATACAATGTCTGGCGAATTACCACCATTGGGACTTCCGCAGACACCGTCTGCAGAATTCCAATCGGCAAAGTTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                   <  1:1355913/150‑1 (MQ=255)
                                                   gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTCCGTCCGTTTACTTTcc                                                                                                   >  2:1880403/1‑150 (MQ=255)
                                                   gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                   <  1:745892/150‑1 (MQ=255)
                                                   gTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTcc                                                                                                   <  2:1317480/150‑1 (MQ=255)
                                                     tGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGGCCGTTTACTTTCCGt                                                                                                 >  1:495880/1‑150 (MQ=255)
                                                         aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                             <  1:836689/150‑1 (MQ=255)
                                                         aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaa                                                                                             <  1:2862896/150‑1 (MQ=255)
                                                         aTTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCGGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTGTCCGTTaaa                                                                                             >  2:618499/1‑150 (MQ=255)
                                                           tCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTaaaaa                                                                                           >  2:563879/1‑150 (MQ=255)
                                                             gccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                         >  2:809313/2‑150 (MQ=255)
                                                             aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                         <  2:2896118/150‑1 (MQ=255)
                                                             aCCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACg                                                                                         <  2:2896168/150‑1 (MQ=255)
                                                              ccAGACATTGTCTGGTGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGc                                                                                        >  2:2158260/1‑150 (MQ=255)
                                                              ccAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGTAGCTGTCTGCCAAATCTTTCGCGTTGCCCAGGTCCACCTTAGTGCGTTTACTTTGCGTTAAAATCgg                                                                                        >  2:3283672/1‑149 (MQ=255)
                                                                 gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGa                                                                                     <  2:4155176/150‑1 (MQ=255)
                                                                 gACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCATGCCCAGGTCCACCAACGGCCGTTTACTTTCCGTTAAAAACGCTGa                                                                                     >  1:1996726/1‑150 (MQ=255)
                                                                      tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                                <  1:3091626/150‑1 (MQ=255)
                                                                      tGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGccc                                                                                >  2:2006999/1‑150 (MQ=255)
                                                                       gTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCAGTCTGCCAAATCATTACCCTTGCCCAGGTCCCCCTACCGCCGTGTACTTTCCGGTAAAAACGCTGACGCCCg                                                                               >  1:1437355/1‑150 (MQ=255)
                                                                          tGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGAAGCTGTCAGCCAAATCTTTCCCCTTGCCCAGGTCCACCAACGTCCGTTTACTTTCCGTTAAAAACGCTGTCGCCCGccg                                                                            >  1:3569053/1‑148 (MQ=255)
                                                                                aTTAACACCATTGGGAAGTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTa                                                                      <  2:495808/150‑1 (MQ=255)
                                                                                   aaCACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGGCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGa                                                                   >  2:2058338/1‑150 (MQ=255)
                                                                                     cacCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaa                                                                 <  2:314698/150‑1 (MQ=255)
                                                                                       ccATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTTCTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGaaaaa                                                               <  2:3149663/150‑1 (MQ=255)
                                                                                         aTTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAaga                                                             >  2:2509916/1‑150 (MQ=255)
                                                                                            gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc                                                          <  2:1522376/150‑1 (MQ=255)
                                                                                            gggAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGAc                                                          <  1:917749/150‑1 (MQ=255)
                                                                                                aTTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTc                                                      <  2:2048912/150‑1 (MQ=255)
                                                                                                 ttCTGCAGACGGTGTCTGCGGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCGTTTTTGTAAAAGAGACGCTcc                                                     >  1:2868968/1‑150 (MQ=255)
                                                                                                 ttCCGCAGACACCGTCTGCAGAATTCCCATCGGCAATGATTTGGCAGACGCTGGCTGCCAAATCTTTACCCATGCCCAGGTCCACCTACGTCCGGTTACTTTCCGGTAAAAACGCGGACGCCCCCCGCTTTTTTGAAAAAGTGAAGCTcc                                                     >  1:3100269/1‑150 (MQ=255)
                                                                                                     gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                                 <  1:2677838/150‑1 (MQ=255)
                                                                                                     gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCtg                                                 <  2:4003793/150‑1 (MQ=255)
                                                                                                     gCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATATTTACCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGGTAAAAACGCTGACGCCCGCAGCGTTTATGAAAAAGAGACGcgccgcgg                                                 >  1:255849/1‑148 (MQ=255)
                                                                                                       aGACACCGTCTGCAGAAGGCCCATCGGCCCAGCTTGGGCAGCCGCTGTCTGCCAAATCTTTCCCCTTGCCCCGGTCCACCTACGTCCGTTTACTGTCCCTTACAAACGCTGACGCCCGCAGCGTTTATGAAAAAGAGACGCTCCGCtgtg                                               <  1:865819/150‑1 (MQ=255)
                                                                                                        gACAGCGTCTGCAGAATTCCCATCGGCAAAGATTTGGATGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                              <  2:2535512/150‑1 (MQ=255)
                                                                                                        gACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                              <  2:3533663/150‑1 (MQ=255)
                                                                                                        gACACCGTCTGCAGAATGCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTgg                                              <  2:3906075/150‑1 (MQ=255)
                                                                                                         acacCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGc                                             <  2:3325687/150‑1 (MQ=255)
                                                                                                           acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg                                           >  2:1484027/1‑150 (MQ=255)
                                                                                                           acCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTg                                           <  1:2724215/150‑1 (MQ=255)
                                                                                                             cGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGt                                         <  1:2785311/150‑1 (MQ=255)
                                                                                                                 tGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGt                                     <  1:2011667/150‑1 (MQ=255)
                                                                                                                  gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                                    <  1:2189662/150‑1 (MQ=255)
                                                                                                                  gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGGCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                                    <  1:3376262/150‑1 (MQ=255)
                                                                                                                  gCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCATTCCCCTTCCCCAGCTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGtt                                    <  1:467877/150‑1 (MQ=255)
                                                                                                                   cAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTc                                   <  2:3633262/150‑1 (MQ=255)
                                                                                                                    aGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCg                                  >  2:3060824/1‑150 (MQ=255)
                                                                                                                     gAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGc                                 <  2:2989817/150‑1 (MQ=255)
                                                                                                                       aTTCCCCTCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATGTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                               <  1:3651943/150‑1 (MQ=255)
                                                                                                                       aTTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCa                               <  1:3214705/150‑1 (MQ=255)
                                                                                                                            cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                          >  2:3351461/1‑150 (MQ=255)
                                                                                                                            cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                          >  2:104449/1‑150 (MQ=255)
                                                                                                                            cATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTag                          <  2:2451947/150‑1 (MQ=255)
                                                                                                                                  cAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGc                    >  2:3158424/1‑150 (MQ=255)
                                                                                                                                  cAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGc                    >  2:1077196/1‑150 (MQ=255)
                                                                                                                                   aaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAACGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCa                   >  2:3334482/1‑150 (MQ=255)
                                                                                                                                   aaaGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCGGTGGCTGGTCTGGTCGCCAGCTAGAGCGGCa                   >  2:2017899/1‑150 (MQ=255)
                                                                                                                                       ctttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt               <  1:281625/149‑1 (MQ=255)
                                                                                                                                       aTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAGAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt               <  1:2760132/150‑1 (MQ=255)
                                                                                                                                       aTTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAAtt               >  2:2879806/1‑150 (MQ=255)
                                                                                                                                        tttGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg              >  2:4266671/1‑150 (MQ=255)
                                                                                                                                        tttGGCAGACAGCGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTg              >  1:597906/1‑150 (MQ=255)
                                                                                                                                          tGGCAGACAGCGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCg            >  2:3857091/1‑150 (MQ=255)
                                                                                                                                           ggCAGACAGCGTCTGCCAAATCTTTGCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTGTTCTGACAACGAGACGCTCCGCGGTGGCTGGGCGGTTCGCCAGCTAGAGCGGCACATTGCgc           >  2:2682749/1‑149 (MQ=255)
                                                                                                                                            gCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCAGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAc          <  2:1928646/150‑1 (MQ=255)
                                                                                                                                             cAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAcc         >  1:1006671/1‑150 (MQ=255)
                                                                                                                                             cAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAcc         >  1:1006668/1‑150 (MQ=255)
                                                                                                                                              aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc        >  2:3470117/1‑150 (MQ=255)
                                                                                                                                              aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc        >  2:2550418/1‑150 (MQ=255)
                                                                                                                                              aGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGAccc        >  1:298895/1‑150 (MQ=255)
                                                                                                                                                  gCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGtt    <  1:3829909/150‑1 (MQ=255)
                                                                                                                                                    tGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGtttt  >  2:2199143/1‑150 (MQ=255)
                                                                                                                                                    tGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGtttt  >  2:2187636/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCCTTGCCCAGGTCCACCTACGTCCGTTTACTTTCCGTTAAAAACGCTGACGCCCGCAGCTTTTATGAAAAAGAGACGCTCCGCTGTGGCTGGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTT  >  NC_000913/3368073‑3368370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: