Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,201 C→G 6.9% intergenic (‑173/+257) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,2010CG 6.9% 896.3 / 4.4 302intergenic (‑173/+257)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base C (159/115);  new base G (2/25);  total (162/140)
Fisher's exact test for biased strand distribution p-value = 1.91e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

TGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCT  >  NC_000913/3392052‑3392349
                                                                                                                                                     |                                                                                                                                                    
tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCCGCCTACGGTTATGTTCAGGATTGTAGGCCTGATAAGACGCAAAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTCAACGCCAAAGCCGACCTAc                                                                                                                                                      >  1:2542118/1‑150 (MQ=18)
tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                                                                                      >  1:1530448/1‑150 (MQ=255)
tGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                                                                                                                      >  1:1942094/1‑150 (MQ=255)
  cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                                    >  1:1995992/1‑150 (MQ=255)
   aGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTAGTAGGCCTGAGACGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTAACCGACCTACggg                                                                                                                                                   >  2:2080641/1‑149 (MQ=18)
      agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                                                                <  2:1146726/150‑1 (MQ=255)
       gagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGAACAGCGTCGCATCAGGCAACGGATGTCGGATGCGGCGTAAACGCCTAATACGACCTACGGTTagg                                                                                                                                               >  2:1496904/1‑148 (MQ=255)
           aTTTTTTCGGGCGGCCGGTTGCGGGGTGACCGCCCAATCCAGCCTCGGGTTTTGTTCGGGTTTTTTGGCCTTATAAGACGCACCGCGTCGCAACCGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                                                                                                           <  1:3653343/150‑1 (MQ=17)
           aTTTTTTACGGGTGCCGGATGGGGCGTGACCGCCCTATCCCGCCTACGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTATGTTct                                                                                                                                           <  2:2129099/150‑2 (MQ=14)
              ttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGtt                                                                                                                                        <  2:187584/150‑1 (MQ=255)
               tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                                                       <  2:3113359/150‑1 (MQ=255)
                 tACGGCTGCCGGATGCGGCGTGAACGCCCTATCCGGCCTACGGTTATGTTCCGGTTAGTAGGCCTGATAAGACGAACAGCGTCGCAGATGGCAACGGCTGGCCGCTGCGGCGTAAACGCGAGCTCCGACCTCGGGGTAGGTTCCggtggt                                                                                                                                     >  2:2804830/1‑145 (MQ=18)
                 tACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCTACCTACGGTTATGTTCCGTTTGt                                                                                                                                     >  2:1548748/1‑150 (MQ=255)
                  ccGGGTGCCGGATGCGGCGTGACCGCCCTACCCTGCCTAGGGTTTTGTTCCGTTTTTTTGGCCTGATAAGACCCACCCCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                                    <  1:2528568/149‑1 (MQ=14)
                  aCGGCTGCCGGATGCGGCTTGAACGCCCTATCCAGCCTACGGTTATGTTCCGTTTTGTCGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                                    <  1:1678003/150‑1 (MQ=255)
                  aCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                                    <  2:3874768/150‑1 (MQ=255)
                   cGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAg                                                                                                                                   <  2:2262007/150‑1 (MQ=255)
                     ggtGCCGGTTGCGGGGTTACCGCCTTATCCAGCCTTGGTTTATGTTCCTTTTTGTATGCTTTTTATGACGAACCGCTTCGAATCAGCAAACGGCTGTCGGATGCGGCTTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                                                                                                                                 <  1:3996668/148‑1 (MQ=9)
                         ccGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGATGTTCGGTTTGTAGGCCTGa                                                                                                                             >  1:2838232/1‑150 (MQ=255)
                              tGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                                                                                                                        <  1:2352039/150‑1 (MQ=255)
                                  gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACCGAGGCGGCGTAAACGCCGTATCCGACCTACGGTGCTGTTCCGAATCTAGGCCTGAGAACACGca                                                                                                                    >  2:2388828/1‑150 (MQ=14)
                                    gTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                                                                                                  <  1:319155/150‑1 (MQ=255)
                                    gTGAACGCCCTATCAAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca                                                                                                                  <  1:3138736/150‑1 (MQ=255)
                                         cGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                                                                                                             >  1:3588584/1‑150 (MQ=255)
                                          gCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTATGCCAGATAAGACGCACAGCCTCg                                                                                                            >  2:2570959/1‑150 (MQ=255)
                                          gCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                                                                                                            >  2:4196544/1‑150 (MQ=255)
                                           cccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTCGATGCCTGTTAACACGCACACAGTCGc                                                                                                           >  2:3496985/1‑150 (MQ=14)
                                           cccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGGCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                           >  1:2495813/1‑150 (MQ=255)
                                            ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                                          >  2:2897556/1‑150 (MQ=255)
                                               acccACCCTCCGGTTATGTTCCGGTTGGACCCCCATAAAACACGCACAGCGACGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACCCACAGCGTCGCATCa                                                                                                       <  1:3789457/148‑1 (MQ=11)
                                                tCCAGCCTCCGTTTATGTTCCGGTTTGTAGGCCTGATACGACGCACTGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                      <  1:1889483/150‑1 (MQ=14)
                                                tCCAGCCTACGTTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                      <  2:3734155/150‑1 (MQ=255)
                                                tCCAGCCTACGGTTTTGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                      <  1:1519637/150‑1 (MQ=255)
                                                tCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATCAGACGCACAGCGTCGCATCAg                                                                                                      >  2:553510/1‑150 (MQ=255)
                                                tCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                      <  1:2029162/150‑1 (MQ=40)
                                                gccAGCCCTCGGTTATGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg                                                                                                      <  1:4135248/149‑1 (MQ=255)
                                                 ccAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAgg                                                                                                     <  2:2665975/150‑1 (MQ=40)
                                                   aGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGATCTAGGCCAGTTAACACGCACACCCTAGCGACAGGCa                                                                                                   >  2:1985762/1‑150 (MQ=11)
                                                      cTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACg                                                                                                <  2:650093/150‑1 (MQ=37)
                                                            ttATTTTCCGGTTTGTAGTCCTGATAAGACGCACAGCGTCGCATCTGTCAACGGCTGTGGGATGCGGGGTAAACGCCTTATCCGACCAAGGTTTTTTTACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                                                                          <  2:306797/150‑1 (MQ=11)
                                                            ttATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTTCGGTTATGTTCCGTTTGTAGGCGTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGcc                                                                                          >  1:391624/1‑150 (MQ=255)
                                                               tGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGCCGCATCCGGCAGCCGTTGCCTGa                                                                                       <  2:3831714/150‑1 (MQ=11)
                                                                gTTCGGTTTTGTTGGCCTTATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTAGGGTTTTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAt                                                                                      <  2:445528/150‑1 (MQ=11)
                                                                 ttCAGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCAGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                                     >  2:170768/1‑150 (MQ=18)
                                                                  tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                                    >  2:3522365/1‑150 (MQ=35)
                                                                  tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                                    >  1:810504/1‑150 (MQ=35)
                                                                  tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgc                                                                                    >  1:1752135/1‑150 (MQ=35)
                                                                    cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                                  >  2:1639750/1‑150 (MQ=33)
                                                                     ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                                 <  2:3666695/150‑1 (MQ=32)
                                                                     ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTGATCCGCCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                                 <  2:2104616/150‑1 (MQ=17)
                                                                     ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                                 >  1:952346/1‑150 (MQ=17)
                                                                     ggTTTGTAGGCCAGTTATTACGCCCAGGGTCGCATTTGGCACCGGGTGTGGTAGGCGGCGTAAACGCCTTAACCGACCTACGGTTATTTCCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                                 <  2:204327/150‑1 (MQ=11)
                                                                      gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCCACGGCTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTGATGTTCCGTGTGTAGGCCTGATAAGACGCACAGCGACGCATCAGGCAACGGCTGCCGGATgcggcg                                                                                >  2:4110146/1‑150 (MQ=11)
                                                                              gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc                                                                        >  1:1052249/1‑150 (MQ=34)
                                                                               ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                                       >  1:3531572/1‑150 (MQ=35)
                                                                               ccTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCt                                                                       >  1:3445938/1‑150 (MQ=35)
                                                                                cTGATAAGACGCCCAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCtt                                                                      <  2:1792898/150‑1 (MQ=25)
                                                                                 tGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCGGGGTCTAGGCCGGTTTAGACGCACAGAGTCGAATCAGGCACCGGCTGCCGGATGCGGCGTAAACGCCttg                                                                     >  2:329322/1‑149 (MQ=11)
                                                                                          gCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTa                                                            >  1:3829191/1‑150 (MQ=37)
                                                                                           cacaGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTAc                                                           >  1:2925772/1‑150 (MQ=37)
                                                                                             caGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGACGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                         >  1:1154040/1‑150 (MQ=37)
                                                                                                     cATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTc                                                 >  2:4190040/1‑150 (MQ=37)
                                                                                                      aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                >  2:944131/1‑150 (MQ=38)
                                                                                                      aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                                >  2:3030932/1‑150 (MQ=38)
                                                                                                      aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCAGACCTACGGTTATGTTcc                                                >  2:2781498/1‑150 (MQ=38)
                                                                                                         aGGCAACGGCTGTCGGAAGCGGCGTAAACCCCATATCCGACCAACGGTTATGTTCCGTTTGTAGGCCTGATAAGACCCACAGCGTCGCATCAGGCAACGGCTGCCGGAAGCGGCGAAAACGCCAAATCCGACCTACGGGTATGTTcagag                                             >  1:4107847/1‑146 (MQ=11)
                                                                                                          ggCAACGGCTGGCGGATGCGGCGTAAACGCCTTAGCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                            <  2:2905707/150‑1 (MQ=255)
                                                                                                           gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTg                                           >  2:2195777/1‑150 (MQ=255)
                                                                                                              aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAg                                        >  2:2460357/1‑150 (MQ=255)
                                                                                                                ggCTGTCGGATGCGGCGTAAACGCCATATCCGACCTACGGTTATGTTCCGTTTGTAGGCCAGATAAGACGCACAGCGGCGCATCATGCACCGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACATACGGTTATGTTCCGTTTGTAGGc                                      >  1:3275750/1‑150 (MQ=14)
                                                                                                                 gCTGTCGGATGGGGCGTAACGGCCTTAACCGACCTCGGTTTTTGTTCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                     <  2:3001285/150‑1 (MQ=14)
                                                                                                                  cTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                    <  2:604561/150‑1 (MQ=255)
                                                                                                                   tGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTg                                   >  1:2767726/1‑150 (MQ=255)
                                                                                                                   tGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGAGGTTCCGTTTGTTGGCCTg                                   >  2:336564/1‑150 (MQ=255)
                                                                                                                    gTCGGAGGGGGCGTAAACGCCTTATCCGACCTAGGGTTATGTTCGGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                  <  1:2950577/150‑1 (MQ=14)
                                                                                                                    gTCGGAGGCGGCGTAAACGCCTTACCCGACATAGGTTTATTTTCCGTTTTTCGGCCTTAAAAGACGCACAGCGTCGCAACAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                                  <  1:459387/150‑1 (MQ=14)
                                                                                                                     tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                 >  1:4228678/1‑150 (MQ=255)
                                                                                                                     tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                                 <  2:725328/150‑1 (MQ=255)
                                                                                                                      cGGATGCGGCTTAAACGCCTTAACCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                <  2:4072374/150‑1 (MQ=255)
                                                                                                                       ggATGGGGCGTAACCGCCTTATCCGCCCTACGGTTATGTTCCGTTTGTAGGCCTGATACGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTGATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                               <  2:1323424/150‑1 (MQ=14)
                                                                                                                       ggATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa                               >  2:483545/1‑150 (MQ=255)
                                                                                                                        gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAg                              >  1:78834/1‑150 (MQ=255)
                                                                                                                         ttGCGGCTTAACCGCCTTAGCCGCCCTCGGGTTTTGTTCCGTTTGTAGGCCTTATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                             <  1:3754145/149‑1 (MQ=14)
                                                                                                                           gcggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                           >  1:1378516/1‑150 (MQ=255)
                                                                                                                            gggcgTAACCGCCTTACCCGACCTAGGGTTATGTTCGGTTTGTTGGCCTTTTAAGACGCACCGCGTCGCAGCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                          <  1:918330/149‑1 (MQ=14)
                                                                                                                            cggagTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGCTTATGTTCCGTTTGTAGGCCTGATAAGACGc                          >  2:2336871/1‑150 (MQ=255)
                                                                                                                             ggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                         >  2:3944490/1‑150 (MQ=255)
                                                                                                                                   aaCGCCTTTGCCGACATAGGGTTTTGTTCGGTTTGTAGGCCTGATAAGACGCACAGGGTCGCATCAGGCAACGGCTGCAGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGt                   <  2:781998/150‑1 (MQ=14)
                                                                                                                                    aCGCCTTATCCGACCTCCGGTTATGTTCCGTTTGCCGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTc                  <  1:1780561/150‑1 (MQ=255)
                                                                                                                                     cGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCg                 >  1:1336064/1‑150 (MQ=255)
                                                                                                                                      gCCTTATCCGACCTACGGTTATGTTCTGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                >  1:2321891/1‑150 (MQ=255)
                                                                                                                                      gCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                >  1:471414/1‑150 (MQ=255)
                                                                                                                                       ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa               >  1:2141507/1‑150 (MQ=255)
                                                                                                                                        cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt              >  1:3354068/1‑150 (MQ=255)
                                                                                                                                          tacccGACCTACGGTTATGGACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa            <  2:1513731/147‑1 (MQ=255)
                                                                                                                                          tATCCGACCTACGTTTATGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa            <  2:868504/150‑1 (MQ=255)
                                                                                                                                           agccGACCTAGGTTTATGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAg           <  1:1229565/148‑1 (MQ=255)
                                                                                                                                               gACCTACGTTTTTTTTCCGTTTGTTGGCCTGTTAAGACGCACAGGGTGGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCaa       <  1:3451703/150‑1 (MQ=11)
                                                                                                                                                aCCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAAc      >  2:139/1‑150 (MQ=37)
                                                                                                                                                  cTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACgg    >  2:614602/1‑150 (MQ=35)
                                                                                                                                                     |                                                                                                                                                    
TGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCT  >  NC_000913/3392052‑3392349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: