Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 5.8% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 5.8% 452.6 / 3.2 171intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (54/103);  new base G (8/6);  total (62/109)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504708‑3505004
                                                                                                                                                   |                                                                                                                                                     
cAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTc                                                                                                                                                     <  2:2639304/150‑1 (MQ=255)
 aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  1:1056922/1‑150 (MQ=255)
 aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  2:2217560/1‑150 (MQ=255)
  aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                   >  1:3906204/1‑150 (MQ=255)
  aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTacc                                                                                                                                                   >  1:2274013/1‑150 (MQ=255)
   aCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTgccc                                                                                                                                                  >  2:1453836/1‑150 (MQ=255)
       gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  2:865335/150‑1 (MQ=255)
       gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  2:1862991/150‑1 (MQ=255)
       gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  1:2529015/150‑1 (MQ=255)
        tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                             <  1:153283/150‑1 (MQ=255)
        tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                             <  2:2911758/150‑1 (MQ=255)
        tGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAcc                                                                                                                                             <  2:2911841/150‑1 (MQ=255)
         gCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAccc                                                                                                                                            >  1:3148721/1‑150 (MQ=255)
         gCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCAccc                                                                                                                                            >  2:1480120/1‑150 (MQ=255)
          cACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCAccca                                                                                                                                           >  2:760602/1‑149 (MQ=255)
             gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTAcccaaacccaaaa                                                                                                                                        >  2:3981791/1‑149 (MQ=255)
             gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGACCGCACCCaaaa                                                                                                                                        >  2:3981796/1‑149 (MQ=39)
                 ccGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctc                                                                                                                                    <  2:2341779/150‑1 (MQ=255)
                 ccGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCCCCCGAAcccac                                                                                                                                    >  2:3432480/1‑148 (MQ=255)
                 ccGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCAAAacccc                                                                                                                                    >  1:2527338/1‑148 (MQ=255)
                  cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctct                                                                                                                                   >  2:2469751/1‑150 (MQ=255)
                  cGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCctct                                                                                                                                   >  1:632303/1‑150 (MQ=255)
                   gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAcccacac                                                                                                                                  >  1:446808/1‑146 (MQ=255)
                     aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGACGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                <  1:1381839/150‑1 (MQ=255)
                       tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACTccccc                                                                                                                              >  1:3823025/1‑149 (MQ=255)
                         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACAACCAAAAACACTACCCaaa                                                                                                                            >  1:1903272/1‑150 (MQ=38)
                         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  2:1077123/150‑1 (MQ=255)
                         ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  1:3381015/150‑1 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCTGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  2:1011444/150‑1 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  2:1822516/150‑1 (MQ=255)
                          tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  1:1191295/150‑1 (MQ=255)
                              ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                       <  1:2194678/150‑1 (MQ=255)
                                gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:1155583/150‑1 (MQ=255)
                                 cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAg                                                                                                                    >  2:3535101/1‑150 (MQ=255)
                                  gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                   <  1:1698524/150‑1 (MQ=255)
                                   ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCCACCCGAAACCACAACCCAAAGGGGCGgggg                                                                                                                  >  1:1879177/1‑150 (MQ=38)
                                   ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGGGCCCACACCCGCCCCCCCGCCCCCCAGGGGCGCggg                                                                                                                  >  1:1879104/1‑150 (MQ=37)
                                     aaTCGCATCCCCTTACCTCTTGATAAGCAAAGACCGTAACCGTAGGCCGGATAAGATGCGCCAGCCTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACCTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  2:4054985/150‑1 (MQ=38)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATTAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  2:3909581/150‑1 (MQ=255)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCTTAGCCTCTCCCCAAAGGGGCggggggt                                                                                                                >  2:1398819/1‑149 (MQ=255)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCAAAACCACAACCCCAAGGGGGGAggggc                                                                                                                >  1:3738009/1‑149 (MQ=38)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  1:4058248/150‑1 (MQ=255)
                                     aaTCGCATCACCTTAACTATTGATAATAAAAGACAGTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCAGGCGATGCTGGCGCGTTGATTTTTACATACCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                <  2:3210770/150‑1 (MQ=39)
                                          cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCGCACACCCAAAGGGCGCGGGGGcccgg                                                                                                           >  1:1112881/1‑147 (MQ=37)
                                           aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTc                                                                                                          <  1:3988048/150‑1 (MQ=255)
                                            tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCa                                                                                                         <  2:1155454/150‑1 (MQ=255)
                                            tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:963268/150‑1 (MQ=255)
                                              aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCGAACCCACTACCCAAAGGGGCGCGGGGGcccggcagt                                                                                                       >  2:1625104/1‑143 (MQ=38)
                                               ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                      <  2:1212338/150‑1 (MQ=255)
                                                      tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAAACCACACCCCAAACGGGCGCGGGGCCCCGTCAGTACCGaaat                                                                                               >  2:3684797/1‑148 (MQ=37)
                                                      tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAAtt                                                                                               >  1:2758273/1‑150 (MQ=255)
                                                           ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                          <  1:3134290/150‑1 (MQ=255)
                                                               taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                      >  2:3162842/1‑150 (MQ=255)
                                                               taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCGTGAc                                                                                      >  2:1042056/1‑150 (MQ=255)
                                                               taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGTTCCCCAAAACCAAACCCACAACCCCAAGGGGGGGCGGGCCCGGGAAGTAACGAATTTATCTTgta                                                                                      >  1:3390784/1‑148 (MQ=19)
                                                                aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                     <  2:3906204/150‑1 (MQ=255)
                                                                aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACg                                                                                     <  2:3658580/150‑1 (MQ=255)
                                                                aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACACCCCAAACGGGCGAGGGGACCCGTCAGTAAGGAATTTAGCATGACg                                                                                     >  1:3896081/1‑150 (MQ=38)
                                                                   aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAAACCACAACCCAAAAGGGCGAGGGGACCCGTGAGTACCGGAATTAGGGTTACGGTg                                                                                  >  2:1524729/1‑150 (MQ=25)
                                                                   aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAACCGAACCCACTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCGTGACGGTg                                                                                  >  2:3532160/1‑150 (MQ=39)
                                                                    gACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAACCCTCACCCCCAAGGGGCGAGGGGACCGTTAAGTACCGAAATTAGCTTGAAGGTGc                                                                                 >  2:2015937/1‑150 (MQ=38)
                                                                       aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGGCCCGCACCCTAAAAAACGCCCCCAAGGGGCGAGGGGCCCGGGCAGTTCGGACTTGATCTTGGCGGttcgag                                                                              >  1:543933/1‑145 (MQ=21)
                                                                        ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  2:3388048/150‑1 (MQ=255)
                                                                          aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  1:1817924/150‑1 (MQ=255)
                                                                          aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  2:1534181/150‑1 (MQ=255)
                                                                           aCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAg                                                                          <  1:144993/150‑1 (MQ=255)
                                                                             cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                        <  2:1297384/150‑1 (MQ=255)
                                                                               tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  1:258139/150‑1 (MQ=255)
                                                                               tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  1:362471/150‑1 (MQ=255)
                                                                                      gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                               <  2:359205/150‑1 (MQ=255)
                                                                                      gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAAACCACTCCCCAAAGGGGCGCGGGGACCGGTCAGTACCGAAGTTAGCGTGACGGTGCTATCCAGCATTaaaaaaa                                                               >  2:980756/1‑146 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGCGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:2464095/150‑1 (MQ=255)
                                                                                       aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  1:2129030/150‑1 (MQ=255)
                                                                                        tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTCCGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:1527891/150‑1 (MQ=255)
                                                                                           gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  1:64860/150‑1 (MQ=255)
                                                                                           gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  2:3574648/150‑1 (MQ=255)
                                                                                           gATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  1:3165472/150‑1 (MQ=255)
                                                                                           gATGCGCCAGCATCGCAACCGGCGATGCAGGCGCGAAGAAATTTACATCCCCAACCTTCCCCACACCCTAACCCTCACCCCAAAGGCGCGAGGGGACCGTTCACTACGGAATTTAGCTTCACGGTGCTATCCAGCATTAAATACTGGCaa                                                          <  2:3872113/150‑1 (MQ=37)
                                                                                                  cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                   <  1:2242535/150‑1 (MQ=255)
                                                                                                   aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  2:3039784/150‑1 (MQ=255)
                                                                                                         gcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                            <  2:546044/150‑1 (MQ=255)
                                                                                                              atgCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCACCATTAAAAAATGGCAATACTCTCACGCAAAATCCg                                       >  2:666387/3‑150 (MQ=255)
                                                                                                               ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAACCCTCTCCCCCAAGGGGCGAGGGGCCCCGTCAGTAAGGAAATTAGCTTTACGGTGCTATACCGCATAAAAAACTGGCAAAACTCTCACCCAAAAtaccc                                      >  2:2991518/1‑146 (MQ=255)
                                                                                                                gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                     >  2:3174593/1‑150 (MQ=255)
                                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCTAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  2:2393502/150‑1 (MQ=255)
                                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  1:1210870/150‑1 (MQ=255)
                                                                                                                       tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  2:3883964/150‑1 (MQ=255)
                                                                                                                            cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                         <  2:307715/150‑1 (MQ=255)
                                                                                                                                   ttttACATCCCGTACGTTACCCACACCCTAACCCACTCCCCCAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTTACGGTGCTATCAAGCAATAAAAAATGGCAAAAAACTCACGCAAAAACCGCAGCACCCTCTCGTCACCGCt                  >  2:435980/1‑150 (MQ=255)
                                                                                                                                           cccGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg          <  2:4036802/150‑1 (MQ=255)
                                                                                                                                           cccGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCg          <  2:778476/150‑1 (MQ=255)
                                                                                                                                           cccGGACGTTCCCCACCCCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACAGTCTCTTCACCGCTGCGATTCg          <  2:2983256/150‑1 (MQ=255)
                                                                                                                                            ccGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg         <  2:3079823/150‑1 (MQ=255)
                                                                                                                                              gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt       <  2:3448214/150‑1 (MQ=255)
                                                                                                                                               tACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGtt      <  2:173572/150‑1 (MQ=255)
                                                                                                                                                  gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  1:1708790/150‑1 (MQ=255)
                                                                                                                                                  gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  1:3340751/150‑1 (MQ=255)
                                                                                                                                                  gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   >  2:716003/1‑150 (MQ=255)
                                                                                                                                                  gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   <  2:1436822/150‑1 (MQ=255)
                                                                                                                                                   ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg  >  2:1362837/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504708‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: