New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3741341 =	120 (2.020)	3 (0.050)	3/266	NT	2.6%	coding (242/474 nt)	ysaA	putative hydrogenase, 4Fe‑4S ferredoxin‑type component
	?	NC_000913	3741376 =	112 (1.960)					coding (207/474 nt)	ysaA	putative hydrogenase, 4Fe‑4S ferredoxin‑type component
Rejected: Frequency below/above cutoff threshold.

GCGAATGTCTGCCCTGTTGACGCGATAAGCCGCGAACATGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3741384‑3741341 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcaGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACACTGCGCAAGCC  >  NC_000913/3741376‑3741523                                                                                                                                                                                                  GCGAATGTCTGCCCTGTTGACGCGATAAGCCGCGAACATGGGCAGACATTCGCGCACGGTGCAGCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAA                                             >  1:801368/1‑149      TGTCTGCCCTGTTGACGCGATAAGCCGCGAACATGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAAAAAACTCGTCTCGTGACAACGCAG                                        >  1:216547/1‑149          TGCCCTGTTGACGCGATAAGCCGCGAACATGGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGTTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGACTGGTGACAACGCAGCGCA                                    >  2:109311/1‑149                                        GGGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCAAGATGCGACACTGCGCA      >  1:844657/1‑149                                         GGCAGACATTCGCGCACGTTGCATCTTCACACTGATGACAGGCAACTGCCGTGGTCCAGCAGAGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACACTGCCCAA     >  1:345198/1‑149                                         GGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGCCAACGCAGCGCAATCCTGATTCTCATGATGCGACACTGCGCAA     >  2:172947/1‑149                                         GGCAGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCCATCCTGATTCTCATGATGCGACACTGCGCAA     >  1:565405/1‑149                                          GCAGACATTCGCGAACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCAAGCAGTGGTCTTTAATGACACGAATACGGGAAAAAAACTCGTCGGGTGACAACGCAGCGCAATCCTCATTCACATGAAGCGTCACAGCCCAAG    >  2:522638/1‑149                                            AGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGAAGCGACACTGCGCAAGCC  <  1:574928/149‑1                                                                                                                                                                                                  GCGAATGTCTGCCCTGTTGACGCGATAAGCCGCGAACATGGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3741384‑3741341 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggcaGACATTCGCGCACGGTGCATCTTCACACTGATGACAGGCTACTGCCGTGGTCCAGCAGTGGTCTTTAATGACACGAATACGGGAAATAAACTCGTCTGGTGACAACGCAGCGCAATCCTGATTCTCATGATGCGACACTGCGCAAGCC  >  NC_000913/3741376‑3741523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.