New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4127164 =	57 (0.960)	3 (0.050)	3/266	NT	4.9%	coding (152/213 nt)	rpmE	50S ribosomal subunit protein L31
	?	NC_000913	4127222 =	61 (1.070)					coding (210/213 nt)	rpmE	50S ribosomal subunit protein L31
Rejected: Frequency below/above cutoff threshold.

GCCAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGTGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACGGCCACCGGTAGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4127315‑4127164 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcaaATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTT  >  NC_000913/4127222‑4127368                                                                                                                                                                                                                                                                                                             GCCAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGGGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACGGCCACCGGTAG                                                                                                                                                        >  1:44590/1‑149  CCAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGTGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACTGCCACCGGTAGa                                                                                                                                                       >  2:711623/1‑148  CCAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGTGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACGGCCACCGGTAGC                                                                                                                                                       >  2:722760/1‑149   CAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGTGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGAATGTTGAAGCGGTCAACACGGCCACCGGTAGCA                                                                                                                                                      >  2:698165/1‑149                                                                                       GGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACGGCCACCGGTAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGC                                                                  >  1:1026520/1‑149                                                                                           TTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACATGGCCACCGGTAGCAAATAAGACCCGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATT                                                              >  2:478353/1‑149                                                                                                        GGGATGTTGAAACGCTTGTTGAAGCGGTCAACA‑‑TCC‑CGGGCAGCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGT                                              >  2:351895/1‑149                                                                                                                                                     GCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGT    >  1:739416/1‑149                                                                                                                                                      CAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTT   >  2:415115/1‑149                                                                                                                                                      CAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGGGGCGCATCGAAAACGGCTTGTGGCGAGACAACCGTT   >  1:1020571/1‑149                                                                                                                                                       AAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTT  <  1:325454/149‑1                                                                                                                                                                                                                                                                                                             GCCAAAGAATGGCCGCTGTTTTCGTGGTAATACGACCAAAATTCAGGCACAAAAAAAGCGCCGTGCGGCGCTTTTTTCGGAAATCCGGTCTTATTTGCTGCCCGGGATGTTGAAACGCTTGTTGAAGCGGTCAACACGGCCACCGGTAGCAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4127315‑4127164 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑agcaaATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGAATTTTGGTCGTATTACCACGAAAACAGCGGCCATTCTTTGGCGACCGTGGTGAGTTGTGGCGCATCGAAAACGGCTTGTGGCGAGACCACCGTTT  >  NC_000913/4127222‑4127368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.