Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 744,457 T→A 7.2% L72Q (CTG→CAG)  ybgK → putative allophanate hydrolase, subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913744,4570TA 7.2% 150.1 / 2.6 62L72Q (CTG→CAG) ybgKputative allophanate hydrolase, subunit 2
Reads supporting (aligned to +/- strand):  ref base T (32/24);  new base A (2/2);  total (34/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

TCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGT  >  NC_000913/744312‑744604
                                                                                                                                                 |                                                                                                                                                   
tCGGGTATCAGCCACTGCGGCGCACTGGAGATGCCCGCGTTACGCATTGCTAAGCTACTGGTGGGTAATGACTCCAATGGCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGAc                                                                                                                                                  <  1:708854/149‑1 (MQ=255)
    gTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGGTTGCTCTGACGGGt                                                                                                                                              <  1:838651/149‑1 (MQ=255)
      aTCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGc                                                                                                                                            <  2:345440/149‑1 (MQ=255)
       tCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGAGGGTAATGACGCCAATGCCCCCGCGCTGGAGATAACGCTCGGTCAGTTAACTGTGGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGcc                                                                                                                                           >  2:367831/1‑149 (MQ=255)
         aGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGCCGCCAATGCCCCCGCGCTGGAGATCCCGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCgg                                                                                                                                         >  2:255715/1‑149 (MQ=255)
         aGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCgg                                                                                                                                         >  2:506093/1‑149 (MQ=255)
         aGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCgg                                                                                                                                         >  2:259595/1‑149 (MQ=255)
          gCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGTGTGCCGGt                                                                                                                                        >  1:433213/1‑149 (MQ=255)
           ccACTGCGGCGCACTGGTTATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCACTGCCCCCGCGCTGGAGATCCCGCTCTGTCAGTTAACTGTTGAGTTCGATACTGGTGGGTGGTTTTCTCTGACGGGTGCCGGtt                                                                                                                                       >  2:987639/1‑149 (MQ=255)
            cACTGCGTCGGACTGTATATGCCGGCGTTTCGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCGCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGTTGGGTGGTTTGCTCTGACGGGTGCCTGTTg                                                                                                                                      <  1:650842/149‑1 (MQ=255)
                      cACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCgg                                                                                                                            >  1:841411/1‑149 (MQ=255)
                       aCTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGc                                                                                                                           <  1:510074/149‑1 (MQ=255)
                        cTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCt                                                                                                                          >  2:551813/1‑149 (MQ=255)
                                     cgTTACGCATTGCTAACCGACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGGCAGTTAACTGTTGAGTTCGAAACTGATGGGGGGTTTGCGCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGcc                                                                                                             <  2:298360/149‑1 (MQ=255)
                                       ttACGCATTCCTAACCTACTGGTGGTTAATGACGCCAATGCCCCCTCGCTGGAGATCACGCTCGGTCAGTTAACTGTTTAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCgg                                                                                                           <  1:923995/149‑2 (MQ=255)
                                           gCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTgg                                                                                                       >  1:552159/1‑149 (MQ=255)
                                                gCTAACCTACTGGTGGGTAATGACGCCAATGACCACGCGATGGAGATCACGCTCGGTCAATTAACTGTTGAGTTCGAAAATGATCGGTGGTTTGCTCTTACGGGTGCCAGTTGCGCTTCGAGGCTGGAAGATAATTCCGTCTGGACCgg                                                                                                  >  2:611277/1‑149 (MQ=255)
                                                 cTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAGGCGCGGCTGGATGATAATGCCGTCTGGACCGGc                                                                                                 >  2:628228/1‑149 (MQ=255)
                                                    aCCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCTGTCAGTTAACTGTTGAGTTCGAACCTGATGGGTGGTTTGCTCTGACGGCTGCCGGTTGCGAAGCGCGGCTCGATGATAATGCAGCATGGACCGGCTgg                                                                                              >  1:683039/1‑149 (MQ=255)
                                                    aCCTACTGGTGGGTAATAACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTgg                                                                                              <  1:539377/149‑1 (MQ=255)
                                                         cTGGCGGGTACTGAGCCCAATGCCCCCGCGGTGGAGCTCACGCGCGGTCAGTTAACTGTGGAGTTCGAAACTGATGGGAGGATTGCTCTGACGGGTGCCGGAGGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGAtt                                                                                         <  2:785304/149‑1 (MQ=255)
                                                         agggggggTAATGACGACAATGCCCCCGCGCTGGAGATCCCGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTTCTCTGACGGGAGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGAtt                                                                                         <  2:789543/144‑1 (MQ=255)
                                                           ggtggGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGc                                                                                       >  2:9317/1‑149 (MQ=255)
                                                           ggtggGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGc                                                                                       <  1:9317/149‑1 (MQ=255)
                                                              gggTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGGTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGa                                                                                    <  1:493128/149‑1 (MQ=255)
                                                              gggTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACAGAACGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCTTCTGGACCGGCTCGCGATTGCCGa                                                                                    >  2:110917/1‑149 (MQ=255)
                                                               gggaaTGACGCCTAGGCCCCCGCGGTGGAGATCACGCGCGGTCAGTTAACTGTGGAGTTCGATAGTGATGGGTGGTTTGCTCTGACGGGTGCCGTTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTTCCGAt                                                                                   <  1:110917/146‑1 (MQ=255)
                                                               ggTAATGACGCCAATGCCCCCGCGCTGGAGACCACGCGCGGTCCGTGAACGGTTGAGTTCGCAACTGATTGGCGGTGTGCTCTGACGGGTTCCGGTTTCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGAt                                                                                   <  2:318233/149‑1 (MQ=255)
                                                                        gCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGGTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCGGGACCGGCTGGCGAGTGCCGATGAAAGCAgg                                                                          >  2:949577/1‑149 (MQ=255)
                                                                         ccAATTCCCCCGGGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTATGCTCTGACTGGTGCCGTTTGCGAAGCTCGGCTGGATGATAATGCCGTCTGGACCGTCTGGCGATTGCCGATGAAAGCAGGc                                                                         <  1:503174/149‑1 (MQ=255)
                                                                         ccAATGCCCCCGCGCTGGAGATCCCGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                         <  1:255715/149‑1 (MQ=255)
                                                                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAAGTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                         >  2:945479/1‑149 (MQ=255)
                                                                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                         <  1:259595/149‑1 (MQ=255)
                                                                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                         <  1:506093/149‑1 (MQ=255)
                                                                            aTGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAg                                                                      >  2:97788/1‑149 (MQ=255)
                                                                            aTGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGAGAAGCGCGGGTGGTTGATAATGCCGTCTGGACCTGCTGGCGTTTGCCGATGAATCCAGGCCAg                                                                      >  1:62233/1‑149 (MQ=255)
                                                                                ccccGCGCTGGAGATCACGCTCGGTCAGTTATCTGTTGAGTTCGAAACTGATGGGTGGTTTGCTGTGACGGGTGCCGGTTGCGATGCGCGGCTGGATGATAATGCCGTCTGGACAGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGtt                                                                  >  2:270773/1‑149 (MQ=255)
                                                                                 cccGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGAGGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGttt                                                                 >  1:377394/1‑149 (MQ=255)
                                                                                 cccGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGAGGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGttt                                                                 >  1:381719/1‑149 (MQ=255)
                                                                                      gcTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGc                                                            <  2:841411/149‑1 (MQ=255)
                                                                                          gagtTCACGCTCGGTCAGTTAACTGTTGAGGGCGAAACTGATGCGTGGTTTTCTCTGACGGGTGCCGGTTGCGAAGCGCGGCGGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTaa                                                        <  1:922929/149‑1 (MQ=255)
                                                                                          gagaTCACGCTCGGGCAGTTAACTTTTGAGTTCGCAACTGTTGGCTGGTTTGCGCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTAAACGCtggc                                                        <  1:922193/149‑4 (MQ=255)
                                                                                                 cggtCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCg                                                 >  2:849900/4‑149 (MQ=255)
                                                                                                     cGGTCAGTTAACTGTTGAGTTCGAAACGGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGACGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTGAACGCGTAAACGCACgcagc                                             >  2:979362/1‑149 (MQ=255)
                                                                                                          acttAACTGTTGAGTTCGAAACTGATGGGTGTTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCCGCTGACGATTGCCGATGAAAGCAGGCCAGCGTTTAACCCTTAAACTCCCGCAGAACggg                                        >  2:678293/3‑149 (MQ=255)
                                                                                                          aGTTAACTGTTGCGTGCGAACCTGACGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACggg                                        <  2:381719/149‑1 (MQ=255)
                                                                                                          aGTTAAATGTTGAGTTCGAAACTGATGGGTGTTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACggg                                        <  2:377394/149‑1 (MQ=255)
                                                                                                            ttAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGACGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGCAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGAt                                      >  1:955243/1‑149 (MQ=255)
                                                                                                            ttAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGAt                                      >  2:467489/1‑149 (MQ=255)
                                                                                                                 tGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCa                                 >  2:397715/1‑149 (MQ=255)
                                                                                                                 tGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCa                                 >  1:878258/1‑149 (MQ=255)
                                                                                                                   ttGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGAT‑ATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACTCCAGCAGCACGGGATGCGCAGtt                              >  2:992225/1‑149 (MQ=255)
                                                                                                                      aGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTAt                            >  2:391878/1‑149 (MQ=255)
                                                                                                                       gatCGAAACTGATGCGGGGTTTGCTCGGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCGGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATc                           <  2:973791/147‑1 (MQ=255)
                                                                                                                                 gATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgc                 >  1:639840/1‑149 (MQ=255)
                                                                                                                                 gATGGGGGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgc                 <  1:642377/149‑1 (MQ=255)
                                                                                                                                  aTGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgcg                >  1:114507/1‑149 (MQ=255)
                                                                                                                                   tGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCgg               <  2:433213/149‑1 (MQ=255)
                                                                                                                                      gtggtTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGGCCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGggtg            >  2:262654/1‑149 (MQ=255)
                                                                                                                                      gaggtTTGCTCTGCCGGGTGCCGGGTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTAGCTGGCGGTCGCGggtg            <  2:873603/147‑1 (MQ=255)
                                                                                                                                       tggtTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGGTATCTGGCGGTCGCGggtgg           >  2:590897/1‑149 (MQ=255)
                                                                                                                                          gttGCCCCGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGCTCATGCCGGCTGGACCGGCTGTCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTAt        <  1:949577/148‑1 (MQ=255)
                                                                                                                                                ctGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGGCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGt  <  1:551813/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCGGGTATCAGCCACTGCGGCGCACTGGATATGCCCGCGTTACGCATTGCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGT  >  NC_000913/744312‑744604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑