Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 744,507 G→A 6.8% G89S (GGC→AGC)  ybgK → putative allophanate hydrolase, subunit 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913744,5070GA 6.8% 145.1 / 2.6 62G89S (GGC→AGC) ybgKputative allophanate hydrolase, subunit 2
Reads supporting (aligned to +/- strand):  ref base G (33/22);  new base A (2/2);  total (37/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.26e-01

GCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGG  >  NC_000913/744360‑744652
                                                                                                                                                   |                                                                                                                                                 
gCTAACCTACTGGTGGGTAATGACGCCAATGACCACGCGATGGAGATCACGCTCGGTCAATTAACTGTTGAGTTCGAAAATGATCGGTGGTTTGCTCTTACGGGTGCCAGTTGCGCTTCGAGGCTGGAAGATAATTCCGTCTGGACCgg                                                                                                                                                  >  2:611277/1‑149 (MQ=255)
 cTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAGGCGCGGCTGGATGATAATGCCGTCTGGACCGGc                                                                                                                                                 >  2:628228/1‑149 (MQ=255)
    aCCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCTGTCAGTTAACTGTTGAGTTCGAACCTGATGGGTGGTTTGCTCTGACGGCTGCCGGTTGCGAAGCGCGGCTCGATGATAATGCAGCATGGACCGGCTgg                                                                                                                                              >  1:683039/1‑149 (MQ=255)
    aCCTACTGGTGGGTAATAACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTgg                                                                                                                                              <  1:539377/149‑1 (MQ=255)
         cTGGCGGGTACTGAGCCCAATGCCCCCGCGGTGGAGCTCACGCGCGGTCAGTTAACTGTGGAGTTCGAAACTGATGGGAGGATTGCTCTGACGGGTGCCGGAGGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGAtt                                                                                                                                         <  2:785304/149‑1 (MQ=255)
         agggggggTAATGACGACAATGCCCCCGCGCTGGAGATCCCGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTTCTCTGACGGGAGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGAtt                                                                                                                                         <  2:789543/144‑1 (MQ=255)
           ggtggGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGc                                                                                                                                       <  1:9317/149‑1 (MQ=255)
           ggtggGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGc                                                                                                                                       >  2:9317/1‑149 (MQ=255)
              gggTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGGTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGa                                                                                                                                    <  1:493128/149‑1 (MQ=255)
              gggTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACAGAACGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCTTCTGGACCGGCTCGCGATTGCCGa                                                                                                                                    >  2:110917/1‑149 (MQ=255)
               gggaaTGACGCCTAGGCCCCCGCGGTGGAGATCACGCGCGGTCAGTTAACTGTGGAGTTCGATAGTGATGGGTGGTTTGCTCTGACGGGTGCCGTTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTTCCGAt                                                                                                                                   <  1:110917/146‑1 (MQ=255)
               ggTAATGACGCCAATGCCCCCGCGCTGGAGACCACGCGCGGTCCGTGAACGGTTGAGTTCGCAACTGATTGGCGGTGTGCTCTGACGGGTTCCGGTTTCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGAt                                                                                                                                   <  2:318233/149‑1 (MQ=255)
                        gCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGGTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCGGGACCGGCTGGCGAGTGCCGATGAAAGCAgg                                                                                                                          >  2:949577/1‑149 (MQ=255)
                         ccAATTCCCCCGGGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTATGCTCTGACTGGTGCCGTTTGCGAAGCTCGGCTGGATGATAATGCCGTCTGGACCGTCTGGCGATTGCCGATGAAAGCAGGc                                                                                                                         <  1:503174/149‑1 (MQ=255)
                         ccAATGCCCCCGCGCTGGAGATCCCGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                                                                         <  1:255715/149‑1 (MQ=255)
                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAAGTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                                                                         >  2:945479/1‑149 (MQ=255)
                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                                                                         <  1:506093/149‑1 (MQ=255)
                         ccAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGc                                                                                                                         <  1:259595/149‑1 (MQ=255)
                            aTGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAg                                                                                                                      >  2:97788/1‑149 (MQ=255)
                            aTGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGAGAAGCGCGGGTGGTTGATAATGCCGTCTGGACCTGCTGGCGTTTGCCGATGAATCCAGGCCAg                                                                                                                      >  1:62233/1‑149 (MQ=255)
                                ccccGCGCTGGAGATCACGCTCGGTCAGTTATCTGTTGAGTTCGAAACTGATGGGTGGTTTGCTGTGACGGGTGCCGGTTGCGATGCGCGGCTGGATGATAATGCCGTCTGGACAGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGtt                                                                                                                  >  2:270773/1‑149 (MQ=255)
                                 cccGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGAGGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGttt                                                                                                                 >  1:381719/1‑149 (MQ=255)
                                 cccGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGAGGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGttt                                                                                                                 >  1:377394/1‑149 (MQ=255)
                                      gcTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGc                                                                                                            <  2:841411/149‑1 (MQ=255)
                                          gagtTCACGCTCGGTCAGTTAACTGTTGAGGGCGAAACTGATGCGTGGTTTTCTCTGACGGGTGCCGGTTGCGAAGCGCGGCGGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTaa                                                                                                        <  1:922929/149‑1 (MQ=255)
                                          gagaTCACGCTCGGGCAGTTAACTTTTGAGTTCGCAACTGTTGGCTGGTTTGCGCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTAAACGCtggc                                                                                                        <  1:922193/149‑4 (MQ=255)
                                                 cggtCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCg                                                                                                 >  2:849900/4‑149 (MQ=255)
                                                     cGGTCAGTTAACTGTTGAGTTCGAAACGGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGACGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTGAACGCGTAAACGCACgcagc                                                                                             >  2:979362/1‑149 (MQ=255)
                                                          acttAACTGTTGAGTTCGAAACTGATGGGTGTTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCCGCTGACGATTGCCGATGAAAGCAGGCCAGCGTTTAACCCTTAAACTCCCGCAGAACggg                                                                                        >  2:678293/3‑149 (MQ=255)
                                                          aGTTAACTGTTGCGTGCGAACCTGACGGGTGGTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACggg                                                                                        <  2:381719/149‑1 (MQ=255)
                                                          aGTTAAATGTTGAGTTCGAAACTGATGGGTGTTTTGCTCAGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCAGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACggg                                                                                        <  2:377394/149‑1 (MQ=255)
                                                            ttAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGACGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGCAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGAt                                                                                      >  1:955243/1‑149 (MQ=255)
                                                            ttAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGAt                                                                                      >  2:467489/1‑149 (MQ=255)
                                                                 tGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCa                                                                                 >  1:878258/1‑149 (MQ=255)
                                                                 tGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCa                                                                                 >  2:397715/1‑149 (MQ=255)
                                                                   ttGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGAT‑ATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACTCCAGCAGCACGGGATGCGCAGtt                                                                              >  2:992225/1‑149 (MQ=255)
                                                                      aGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTAt                                                                            >  2:391878/1‑149 (MQ=255)
                                                                       gatCGAAACTGATGCGGGGTTTGCTCGGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCGGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATc                                                                           <  2:973791/147‑1 (MQ=255)
                                                                                 gATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgc                                                                 >  1:639840/1‑149 (MQ=255)
                                                                                 gATGGGGGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgc                                                                 <  1:642377/149‑1 (MQ=255)
                                                                                  aTGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTcgcg                                                                >  1:114507/1‑149 (MQ=255)
                                                                                   tGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCgg                                                               <  2:433213/149‑1 (MQ=255)
                                                                                      gtggtTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGGCCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGggtg                                                            >  2:262654/1‑149 (MQ=255)
                                                                                      gaggtTTGCTCTGCCGGGTGCCGGGTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTAGCTGGCGGTCGCGggtg                                                            <  2:873603/147‑1 (MQ=255)
                                                                                       tggtTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGGTATCTGGCGGTCGCGggtgg                                                           >  2:590897/1‑149 (MQ=255)
                                                                                          gttGCCCCGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGCTCATGCCGGCTGGACCGGCTGTCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTAt                                                        <  1:949577/148‑1 (MQ=255)
                                                                                                ctGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGGCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGt                                                  <  1:551813/149‑1 (MQ=255)
                                                                                                  gACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTc                                                >  2:413679/1‑149 (MQ=255)
                                                                                                       gTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCCGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTTTCTGGCGGTCGCGGGTGGTAGTGATGTTccgccg                                           >  1:208831/1‑149 (MQ=255)
                                                                                                           cGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGACTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCCGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGaaa                                       >  1:355828/1‑149 (MQ=255)
                                                                                                             gTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATg                                     >  2:715414/1‑149 (MQ=255)
                                                                                                                   aaGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACTCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCa                               >  2:288122/1‑149 (MQ=255)
                                                                                                                     tcgcgGCTGGATGATACTGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATg                             <  2:251635/148‑1 (MQ=255)
                                                                                                                             ggATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGCTGCGCAGTTATCTGGCGGGCGCGGGTGGTTTTGATGTTCCGCCGGTAATGGGCTCATGCCGCCCCg                     >  2:773196/1‑149 (MQ=255)
                                                                                                                              gatgatAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGCTGCGCAGGTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCCCCgc                    >  2:742048/1‑148 (MQ=255)
                                                                                                                                 tacaaTGACGGCTGGACCTGCTGGCCATTCACGATGAAAGCCGGCAAGCGATTAACGCTATCACGCCCGCAGCCCGGGAGGCGCAGTTTTCTGGCGGTCGCGGGTGGTATTGCTGTTCCGCCGGTAATGGGCTCATGCAGCACCGAtct                 <  2:576910/146‑1 (MQ=255)
                                                                                                                                   tAATGCCGTATGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTGGCGGGTGGTATTGATGTTCCGCCGGTATTGGGCTCATGCAGCACCGATCTCa               >  2:929404/1‑149 (MQ=255)
                                                                                                                                    actGCCGTCTGGACCGGCTGGCGAATGCCGATGAAAGCAGGCCAACGTTTAACGCTTTAACGCCCGCAGCACGGGATGCGCAGATATCTGTCGGTCGCCGGTGGTATTTATGTTACGCCGGTAATGGGCTCAAGCAGCACCGATCTCaa              >  2:693455/3‑149 (MQ=255)
                                                                                                                                           tCTGGACCGGCTGGCTATTGACGCTGATAGCAGGCCAGCGGTTACGGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGGGGGATTGATGTTCCGCCGGTAAAGGGCTGATGGAGCACCGATCTCACAGTggtg       <  1:611277/149‑3 (MQ=255)
                                                                                                                                               gACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCCGCACCGATCTCAAAGTGGGGATTg   >  2:448555/1‑149 (MQ=255)
                                                                                                                                               gACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTg   >  1:530427/1‑149 (MQ=255)
                                                                                                                                                aCCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTgg  >  1:416948/1‑149 (MQ=255)
                                                                                                                                                aCCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCAGGTAATGGGCTCATGCAGCACCGATCTCAAAGGGGGGTTTgg  >  1:821778/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCTAACCTACTGGTGGGTAATGACGCCAATGCCCCCGCGCTGGAGATCACGCTCGGTCAGTTAACTGTTGAGTTCGAAACTGATGGGTGGTTTGCTCTGACGGGTGCCGGTTGCGAAGCGCGGCTGGATGATAATGCCGTCTGGACCGGCTGGCGATTGCCGATGAAAGCAGGCCAGCGTTTAACGCTTAAACGCCCGCAGCACGGGATGCGCAGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGG  >  NC_000913/744360‑744652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑